BIO 208 Master Syll

Central Michigan University
College of Science and Technology
Credit (Mode)
Bulletin Description
The biology of microorganisms: viruses, bacteria, actinomycetes, fungi, algae, and
BIO 101 or BIO 105
Rationale for Course Level
This course is a beginning level microbiology course dependent upon a general
knowledge of biology.
Textbooks and Other Materials To Be Furnished by the Student
Textbook: Tortora, G.J., B.R. Funke, and C.L. Case. 2001. Microbiology. An
Introduction. 7th ed. Benjamin/Cummings Publishing Co., Inc. Redwood City, CA.
Laboratory Manual: Course Pack.
Lens paper (not Bibulous paper), microscope slides with cover slips, colored pencils,
Sharpie marker (black or blue ink).
Optional: Alexander, S.K. and D. Strete. 2001. Microbiology A Photographic Atlas for
the Laboratory. Benjamin/Cummings Publishing Co., Inc. Redwood City, CA.
Special Requirements of the Course
General Methodology Used in Conducting the Course
Two 50 minute lectures and two 80 minute laboratories per week.
Course Objectives
After completing this course the student should be able to:
1. Demonstrate the correct use of a compound light microscope and be able to identify
basic morphologies of bacterial, fungi, algae, and protozoa.
2. Prepare stains of various types of microorganisms.
3. Describe colony morphology and growth characteristics of bacterial and fungal
cultures on various types of media. Provide the rationale for aseptic technique and be
able to transfer bacteria from one form of culture media to another.
4. Distinguish between selective and differential media and provide an application for
the use of various types of media.
5. Identify and describe various means of controlling microbial growth.
6. Perform basic molecular biology techniques related to microbiology.
7. Conduct basic testing procedures used in water and food quality analysis.
8. Discuss ways in which microbiology is involved in everyday life.
VIII. Course Outline
BIO 208 MICROBIOLOGY - Tentative Lecture Schedule
W 1/10
M 1/15
W 1/17
M 1/22
W 1/24
M 1/29
W 1/31
M 2/5
W 2/7
M 2/12
W 2/14
M 2/19
W 2/21
M 2/26
W 2/28
M 3/12
W 3/14
M 3/19
W 3/21
M 3/26
W 3/28
M 4/2
W 4/4
M 4/9
W 4/11
M 4/16
W 4/18
M 4/23
W 4/25
M 4/30
Lecture Topic
Unit 1. The Microbial World and You
CH 1: What Are Microbes? What is Microbiology?
CH 1: How Did Microbiology Become a Science?
Classification of Microbes / CH 3: How Do We See Microbes?
CH 4: Anatomy of Prokaryotic Cells
CH 4: Origin of Eukaryotic Cells / End Material For Test 1
Unit 2. How Do Microbes Grow?
CH 6: Characteristics of Microbial Growth
CH 6: Effects of the Physical Environment on Microbial Growth
CH 7: Control of Microbial Growth / End Material for Test 2
Unit 3. What Enables Microbes to Thrive?
CH 5: General characteristics of metabolism, enzymes, redox
CH 5: How to Get Carbon and Energy from Organic Chemicals;
Carbohydrate catabolism
CH 5: Fermentation / Aerobic Respiration
CH 5: Anaerobic Respiration / Autotrophy
CH 5: Using Energy - Anabolism / End Material for Test 3
Spring Break
Unit 4. Microbial Genetics and Viruses
CH 8: Nucleic Acids / How Prokaryotes Replicate DNA
CH 8: Converting DNA into RNA and Proteins
CH 8: Gene Regulation in Prokaryotes/ Genetic Transfer
CH 13: Viruses - General Characteristics and Bacteriophages
CH 13: Viruses - Animal Viruses
Animal viruses continued / End Material for Test 4
Unit 5. Survey - Role of Microbes in Health and Disease
CH 11: Bacteria, Protozoa, Fungi - introduction
CH 27: Environmental Microbiology
CH 28: Industrial Microbiology
CH 21: Important microbial diseases of the skin; CH 22: nervous
system; CH 23: cardiovascular and lymphatic systems
CH 24: Important microbial diseases of the respiratory system
CH 26: Important diseases of the urinary tract and STDs
CH 25: Important microbial diseases of the digestive system; food
poisoning / The threat of biological warfare
Where is Microbiology Going in the 21st Century?
Final Exam - Comprehensive 10:00-11:50 Brooks 176
Reading in Text
pp. 2-3, 13-22
pp. 3-13; 411-412
pp. 277-285 / pp. 56-68
pp. 77-80; 90-98
pp.82-90; 69-73
pp. 99-108
pp. 140-145; 170-179
CH 7: entire
pp. 113-123
pp. 123-125
pp. 133-135/125-132
pp. 130-132/137-140
pp. 145-149
pp. 48-51/211-215
pp. 215-220
pp. 220-226/233-242
pp. 371-386
pp. 386-397
CH 11: entire (skim);pp.331-341; 349354
CH 27: entire
CH 28: entire
BIO 208 MICROBIOLOGY - Tentative Laboratory Schedule
W, R 10, 11
M, T 15,16
W, R 17,18
M, T 22,23
W, R 24,25
M, T 29, 30
W, R 31,1
M, T 5,6
W, R 7,8
M, T 12,13
W, R 14,15
M, T 19,20
W, R 21,22
M, T 26,27
W, R 28,29
M, T 12, 13
W, R 14, 15
M, T 19,20
W, R 21,22
M, T 26,27
W, R 28,29
M, T 2,3
W, R 4,5
M, T 9,10
W, R 11,12
M, T 16,17
W, R 18,19
M, T 23, 24
W, R 25, 26
M April 30
Laboratory Check-in. Unit 1. Microbes are Everywhere - Start Ex. 1.
Finish Ex. 1. Making Media - Start Ex. 2.
Finish Ex. 2 and collect report pages for Exercises 1 and 2. Observing Microbes - Start and complete Ex.
Quiz 1 Labs 1-4. Collect Ex. 3. Aseptic Technique and Simple Staining - Start & complete Ex. 4.
Collect Ex. 4. Gram Staining - Start Ex. 5.
Collect Ex. 5. Acid fast and Structural Stains - Start and complete Ex. 6. TAs  Ex. 6 but will not collect.
All graded exercises will be returned. End Material For Test 1 (covers labs 1-6).
Unit 2. Growing Bacteria - Start Ex. 7.
Complete and collect Ex. 7.
Control of Microbial Growth - Start Ex. 8.
Quiz 2 Labs 7 and 8. Complete and collect Ex. 8. End Material for Test 2 (covers labs 7 and 8).
Unit 3. Carbohydrate Catabolism - Start
Complete and collect Carbohydrate Catabolism. Fermentation - Start - Inoculate Enterotube II only - 1
per group.
Continue Fermentation - Interpret Enterotube II, Inoculate IMViC test media. Respiration - Start.
Complete and collect Fermentation. Complete and collect Respiration. Autotrophy - Start Ex. 19.
Complete Ex. 19. TAs  Ex. 19 but will not collect. All graded exercises returned. End Material For
Test 3 (covers labs 8, Catabolism, Respiration, and 19).
Unit 4. Isolation of DNA - Start and complete Ex. 11. Electrophoresis of DNA - Start Ex. 12 (pour gels).
Complete Ex. 12.
Collect Ex. 11 and 12 (1 report page). Transfer of Genetic Information - Start Ex. 13.
Complete and collect Ex. 13. Bacteriophage - Start Ex. 14.
Quiz 3 Labs 11-14. Complete and collect Ex. 14 (returned W,R). All other graded exercises returned.
End Material for Test 4 (covers Labs 11-14).
Unit 5. Representative bacteria, fungi, and protozoa - Start Ex. 15 and 20.
Complete Ex. 15 and 20. Microbes in Soil and Water - Start Ex. 16.
Continue Ex. 16, collect Part A. Collect Ex. 15 and 20.
Continue Ex. 16. Food Microbiology - Start Ex. 17.
Quiz 4 Labs 15-16. Complete and collect Ex. 16 Part B. Continue Ex. 17. Microbes of the Skin - Start
Continue Ex. 18, Microbes of the Respiratory Tract
Complete and collect Ex. 17.Complete and collect Ex.18. Start Urinalysis and Microbes of STDs - Ex. 10
Complete Ex. 10.
Collect Ex. 10. Laboratory check-out. All graded exercises can be picked up at your TAs office Friday
April 27.
FINAL EXAM - COMPREHENSIVE. 10:00- 11:50 am in Brooks 176
Best 3 out of 4 tests - 55%
Final comprehensive exam - 10%
Laboratory Grade - 35%
Introductory General Microbiology Textbooks:
Alcomo (2001). Fundamentals of Microbiology. 6th ed. Jones and Bartlett, Boston,
Atlas (1997). Principles of Microbiology. 2nd ed. Wm. C. Brown, Chicago,IL.
Nester et al. (2001). Microbiology: A Human Perspective. 3rd ed. McGraw Hill,
Madison, WI.
Talaro and Talaro (1999). Foundations in Microbiology. 3rd ed. McGraw Hill, Madison,
Advanced General Microbiology Textbooks:
Madigan et al. (2000). Brock Biology of Microorganisms. 9th ed. Prentice Hall. Upper
Saddle River, NJ.
Prescott, et al. (2001). Microbiology 5th ed.
Salyers and Whitt. (2001) Microbiology: Diversity, Disease, and the Environment.
Fitzgerald Science Press, Bethesda, MD.
Medical Microbiology References:
Bailey and Scott's Diagnostic Microbiology. Betty A. Forbes. 1998. QR67.B37 1998
Cellular microbiology: Bacteria-host interactions in health and disease. Brian Henderson, et
al. 1999.
Compact American Medical Dictionary. 1998. R121.C665 1998.
Harrison's Principles of Internal Medicine. 1994. RC46.H333 1994.
Medical Microbiology. Patrick R. Murray. 1998. QR46.M4683 1998.
Microbiology. T. Stuart Walker. 1998. QR46.W245 1998.
Microbiology in Clinical Practice. D.C. Shanson. 1999. QR46.S478 1999.
The American Heritage Stedman's Medical Dictionary. 1995. R121.A53 1995.
Zinsser Microbiology. Wolfgang K. Joklik, ed. 1992. QR46.26 1992
Environmental Microbiology Textbooks:
Atlas and Bartha. (1998). Microbial Ecology. 4th ed. Benjamin Cummings. New
York, NY.
Maier et al (1999). Environmental Microbiology. Academic Press. Boston, MA.
Mitchell. (1992). Environmental Microbiology. Wiley-Liss. New York, NY
Specialty Publications:
Holt, J.G.; Krieg, N.R.; Sneath, P.H.A.; Staley, J.T.; Williams, S.T. (1995) Bergey's
Manual of Determinative Bacteriology, 9th Edition, Williams and Wilkins, Baltimore.
Murray, P.R.; Baron, E.J.; Pfaller, M.A.; Tenover, F.C.; Yolken, R.H. (1995) Manual of
Clinical Microbiology, 6th Ed. ASM Press, Washington, DC.
Internet Sites:
American Society for Microbiology.
Centers for Disease Control and Prevention (CDC).
CDC Morbidity and Mortality Weekly Report.
World Health Organization.
Advances in Microbial Ecology
Applied and Environmental Microbiology
ASM News
Environmental Science and Technology
FEMS Microbial Ecology
Journal of Clinical Microbiology Reviews
Limnology and Oceanography
Microbial Ecology
Microbiological Reviews
Scientific American
Intimate Strangers: Unseen Life on Earth.
Popular Publications:
The Coming Plague: Newly Emerging Diseases in a World out of Balance by Laurie
Garrett. 1994. Penguin Books USA Inc. New York. 750 pages. $14.95 paperback.
ISBN 0 14 02.5091 3. Also available in hardback.
The Hot Zone by Richard Preston. 1994. Anchor Books Doubleday. New York. 422
pages. ISBN 0-385-47956-5.
Breakout: The Evolving Threat of Drug-Resistant Disease by Marc Lappe. 1995. Sierra
Club Books. San Francisco. 255 pages. ISBN 0-87156-382-7.
When Plague Strikes by James Cross Giblin. 1995. Harper Collins Publishers Inc. New
York. 212 pages. ISBN 0-06-446195-5.
Syllabus prepared by
Elizabeth W. Alm
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