Flower parts of lily: 3 sepals, 3 petals, 6 anthers each with 4 pollen chambers, and ovary with compound pistil (3 carpels) Angiosperm Life Cycle © Kwantlen.ca/Horticulture Microscope slide of lily flower bud c.s.: 3 sepals, 3 petals, 6 stamens, 1 ovary Angiosperm Life Cycle © Kwantlen.ca/Horticulture 300 um 600 um 100 um 80 um Anther chamber close-up in early stages of development showing diploid (2n) microsporocytes which undergo meiosis to form four genetically distinct haploid nuclei (1n) Angiosperm Life Cycle © Kwantlen.ca/Horticulture Mature pollen from the lily: exine and one aperture (monocot) Angiosperm Life Cycle © Kwantlen.ca/Horticulture 10 um 500 um Style (longitudinal section) with germinating pollen grains on the stigma Angiosperm Life Cycle © Kwantlen.ca/Horticulture Ovary and Ovule close-ups showing two stages of meiosis which will result in four genetically distinct haploid nuclei. Three nuclei will degenerate. The remaining nuclei will undergo mitosis (occurs three times) to form 8 haploid nuclei (genetially identical) in an “embryo sac” Angiosperm Life Cycle © Kwantlen.ca/Horticulture 1 2 3 4 5 6 7 1. Megasporocyte (diploid) 2. Meiosis telephase I 3. Meiosis telephase II (four megaspores – all genetically distinct) 4. One functional megaspore (n) remains, three other degenerate 5. Megaspore divides by mitosis 6. Nuclei divide again by mitosis 7. Mitosis takes place once more resulting in 8 nuclei that move to different parts of the embryo sac cell (and may later become cells themselves): the two central cell nuclei will be fertilized by one sperm and fuse to form 3N endosperm nuclei (double fertilization); the egg cell will be fertilized to become the zygote and later the embryo; the two synergids (on either side of egg) will degenerate as will the three antipodals at the other end of the embryo sac Embryo sac development in Clematis (typical of most dicots) Angiosperm Life Cycle © Kwantlen.ca/Horticulture Dicot flower model with simple pistil: ovary, ovule, pollen tube, embryo sac (double fertilization: two central cell nuclei will be fertilized by one sperm and fuse to form 3N endosperm nuclei, the egg cell will be fertilized to become the zygote and later the embryo; the two synergids on either side of egg will degenerate as will the three antipodals at the other end of the embryo sac) Angiosperm Life Cycle © Kwantlen.ca/Horticulture Dicot flower model with compound pistil: stigma, style, pollen tube, ovary, ovule Angiosperm Life Cycle © Kwantlen.ca/Horticulture Immature seed l.s. with embryo (arrow) surrounded by endosperm tissue (most angiosperm plants form 3n endosperm) Angiosperm Life Cycle © Kwantlen.ca/Horticulture Seeds (mature ovules) inside mature capsules of lily. The seed coat derived from the integuments. The capsule pericarp is the ripened ovary wall. Angiosperm Life Cycle © Kwantlen.ca/Horticulture Self test #1: What are the arrows pointing at? Angiosperm Life Cycle © Kwantlen.ca/Horticulture