CONTENTS
01
CONTENTS
FOOD
SHEET NO. TITLE
PAGE
SHEET NO. TITLE
PAGE
F001
Separation of Water-soluble Vitamins - 1
05
F069
Separation of Acesulfame K
25
F002
Separation of Water-soluble Vitamins - 2
05
F080
Separation of Aspartame and Acesulfame K in breath mint
25
F067
Separation of Vitamins
06
F049
Separation of Saccharin & Sorbic Acid
25
F005
Separation of Fat-soluble Vitamins
06
F076
Separation of Cyclamic acid
25
F070
Separation of Vitamin C
07
F051
Separation of Pyrazines
26
F003
Separation of Vitamin D2, D3
07
F089
Separation of Ubiquinone 9,10
26
F057
Separation of Tocopherols
07
F090
Separation of DL-Thioctic acid
26
F058
Separation of Vitamin C and E
08
F091
Separation of Benzoyl Peroxide in Flour
26
F097
Separation of Vitamin B12 in Health food
08
F053
Separation of Caffeine and Catechins
27
F071
Separation of Carotenoids
08
F054
Separation of Commercial Tea Drink
27
F004
Separation of β-Carotene in Broccoli
09
F055
Separation of Catechins
28
F059
Separation of β-Carotene
09
F056
Separation of Catechins, Caffeine, Chlorophyll a
28
F007
Separation of Synthetic Antibiotics (Quinolone Derivatives)-1
09
F063
Separation of Allyl isothiocyanate
29
F008
Separation of Synthetic Antibiotics (Quinolone Derivatives)-2
10
F064
Separation of Quassins
29
F009
Separation of Synthetic Antibiotics (Sulfa drugs)
10
F065
Separation of Capsaicins
29
F010
Separation of Synthetic Antibiotics (Furan Derivatives)
11
F082
Separation of Curcumin
29
F011
Separation of Synthetic Antibiotics (Protozoicides)
11
F083
Separation of Curcumin in a commercial drink by LC/MS
30
F012
Separation of Synthetic Antibiotics (Protozoicide & Quinolone)
11
F078
Separation of Rutin and Quercetin
30
F013
Separation of 18 Synthetic Antibiotics
12
F099
Separation of Apigenin
30
F026
Separation of Carbadox
13
F100
Separation of 5,6-Benzoflavone
30
F027
Separation of a Metabolite of Carbadox
13
F101
Separation of 7,8-Benzoflavone
31
F028
Separation of Sulfadimidine
13
F102
Separation of Biochanin A
31
F006
Separation of Tetracycline Antibiotics
13
F103
Separation of Chrysin
31
F014
Separation of Drugs for Animals -1
14
F104
Separation of Diosmin
31
F015
Separation of Drugs for Animals -2
14
F105
Separation of Flavonol
32
F019
Analysis of Oxytetracyclne in Pork
14
F106
Separation of Hesperetin
32
F016
Analysis of α-Trenbolone in Pork
15
F107
Separation of Hesperidin
32
F017
Analysis of β-Trenbolone in Pork
15
F108
Separation of Isoquercitrin
32
F021
Analysis of Zeranol in Pork
15
F109
Separation of Kaempferol
33
F018
Analysis of lvermectin in Pork
15
F110
Separation of Luteolin
33
F020
Analysis of Closantel in Pork
16
F111
Separation of Naringenin
33
F022
Analysis of Flubendazole in Pork
16
F112
Separation of Naringin
33
F029
Separation of Thiabendazole
16
F113
Separation of Rhoifolin
34
F030
Separation of a Metabolite of Thiabendazole
16
F114
Separation of 4,2',4'-Trihydroxychalcone
34
F023
Separation of Albendazole
17
F066
Separation of Oligosaccharide
34
F024
Separation of a Metabolite of Albendazole
17
F068
Separation of Carbohydrates
34
F025
Separation of Isomethamidium
17
F032
Separation of di-Carbonic Acids - 1
35
F031
Separation of Avoparcins
17
F092
Separation of di-Carbonic Acids - 2
35
F044
Separation of Preservatives - 1
18
F035
Separation of Organic Acids - 1
35
F047
Separation of Preservatives - 2
18
F093
Separation of Organic Acids – 2
35
F075
Separation of Preservatives - 3
18
F036
Separation of Organrc Acids - 3
36
F043
Separation of Preservatives - 4
18
F094
Separation of Organic Acids - 4
36
F074
Separation of Preservatives - 5
19
F098
Separation of Organic Acids - 5
36
F077
Separation of Preservatives - 6
19
F062
Separation of Formic acid and Tartaric acid
36
F061
Separation of Preservatives and Saccharin
20
F033
Separation of Oxalic Acid and Nitric Acid in Spinach
37
F042
Separation of Antioxidants - 1
20
F034
Separation of Aromatic Acids -1
37
F073
Separation of Antioxidants - 2
20
F096
Separation of Aromatic Acids -2
37
F048
Separation of Ascorbic Acid & Erythorbic Acid
21
F095
Separation of Aromatic Acids -3
37
F045
Separation of anti-Fungal Preservatives - 1
21
F084
Separation of Malachite green and Leucomalachite green
38
F046
Separation of anti-Fungal Preservatives - 2
21
F085
Separation of Food Dyes - 1
21
Separation of Malachite green and Leucomalachite green by
LC/MS
38
F037
F038
Separation of Food Dyes - 2
22
F086
Separation of Sudan
38
F039
Separation of Food Dyes - 3
22
F087
Separation of Sudan by LC/MS
39
F040
Separation of Food Dyes - 4
23
F052
Separation of Aflatoxin G1, G2, B1, B2
39
F041
Separation of Food Dyes - 5
23
F081
Separation of Fumonisins
40
F072
Separation of Food Dyes - 6
24
F088
Separation of Melamine
40
F060
Separation of Anthocyanins
24
F079
Separation of Putrefactive amines
40
F050
Separation of Aspartame in a Beverage
24
CONTENTS
01
CONTENTS
ENVIRONMENT
SHEET NO. TITLE
PAGE
E001
Separation of N-Methylcarbamate derivatives- 1
E002
Separatiori of N-Methylcarbamate derivatives- 2
43
43
E003
Separatron of Oxine-Copper and Asulam
44
E004
Separation of Oxine-Copper and Thiram
44
E005
Separation of Simazine, Thiram and Thiobencarb
44
E006
Separation of Thiram, Iprodione and Bensulide
44
E007
Separation of Thiram
45
E008
Separation of Thiophanate-methyl and its metabolite
45
E009
Separation of Fenitrothion and its metabolite
45
45
E011
Separation of Agricultural Chemicals (Germicides)
E012
Separation of Agricultural Chemicals (Pesticides)
46
E013
Separation of Agricultural Chemicals (Herbicides)- 1
46
E014
Separation of Agricultural Chemicals (Herbicides)- 2
46
E015
Separation of 5 Agricultural Chemicals - 1
46
E016
Separation of 5 Agricultural Chemicals - 2
47
E028
Separation of 5 Agricultural Chemicals - 3
47
E029
Separation of 5 Agricultural Chemicals - 4
47
E034
Separation of 5 Agricultural Chemicals - 5
47
48
E017
Separation of 6 Agricultural Chemicals
E018
Separation of 9 Agricultural Chemicals - 1
48
E030
Separation of 12 Agricultural Chemicals
48
E035
Separation of 9 Agricultural Chemicals - 2
49
E036
Separation of 3 Agricultural Chemicals
49
E045
Separation of Pesticides standard solution 24【A golf course
pesticide】
50
E019
Separation of Intermediates of Agricultural Chemicals
(halogenized Benzoic acids)
50
E027
Separation of Carbofuran
50
E032
Separation of Acephate
51
E033
Separation of Imazalil in Concentrated Lemonade Juice
51
E040
Separation of Pesticides standard solution 28
51
E041
Separation of 3 Agricultural Chemicals
51
E042
Separation of Carbaryl
52
E043
Separation of Diquat
52
E044
Separation of Iminoctadine triacetate
52
E039
Separation of LAS
52
E010
Separation of 15 Synthetic Antibiotics
53
53
E020
Separation of Benzo[a]pyrene
E023
Separation of Microsystins
53
E046
Separation of Patulin
54
E024
Separation of Bisphenol A -1
54
E025
Separation of Bisphenol A -2
54
E026
Separation of Trace Level Bisphenol A
54
E031
Separation of 4-Vinylguaiacol
55
E021
Separation of DNPH-Aldehydes-1
55
E022
Separation of DNPH-Aldehydes-2
55
E037
Separation of DNPH-Aldehydes-3
56
E038
Separation of DNPH-Aldehydes-4
56
Concerning Mightysil RP-18 GP series
All columns with the "GP" title include the batch certificate.
The guaranteed content is as follows.
1. The physical properties of the packing materials, and carbon content after bonding
2. Analytical results and Test-chromatograms of a separation with our own standard of test-mixtures
Difference in entitling "GP"or not is only including a batch certificate or not.
In addition, they are made with the same packing material.
02 CONTENTS
FOOD 03
04 FOOD
F001
Separation of Water-soluble Vitamins - 1
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 20mM Phosphoric acid including 25mM of Sodium
hexanesulfonate = 10/ 90
Flow Rate
1.0mL/min.
Detection
UV210nm
Temperature
40°C
Sample Vol.
5μL
Samples
1. Nicotinic acid
50μg/mL
2. Nicotinamide
50μg/mL
0.16AUFS
3. Riboflavin phosphate sodium salt
50μg/mL
4. Pyridoxine hydrochloride (Vitamin B6)
200μg/mL
5. Riboflavin (Vitamin B2)
150μg/mL
6. Folic acid (Vitamin B9)
50mg/mL
7. Thiamin hydrochloride(Vitamin B1)
150μg/mL
F002
Separation of Water-soluble Vitamins - 2
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 20mM Phosphoric acid including 25mM of Sodium
hexanesulfonate = 10/ 90
Flow Rate
1.0mL/min.
Detection
UV210nm
Temperature
40°C
Sample Vol.
5μL
Samples
1. Pantothenic acid calcium salt
0.08AUFS
160μg/mL
2. Nicotinamide
20μg/mL
3. Pyridoxine hydrochloride (Vitamin B6)
40μg/mL
4. Riboflavin(Vitamin B2)
80μg/mL
FOOD 05
F067
Separation of Vitamins
Column
Mightysil RP-18GP Aqua 250-4.6mmØ 5μm
Eluent
0.1% Phosphoric acid containing 10mM Sodium hexanesulfonate/
Acetonitrile = 90/10
Flow Rate
1.0mL/min.
Detection
UV210nm
Temperature
35°C
Sample Vol.
5μL
Samples
1. Nicotinic acid
0.05mg/mL
2. Pantothenic acid Calcium salt
0.3 mg/mL
3. Nicotinamide
0.05mg/mL
4. Riboflavin phosphate Sodium salt
0.2 mg/mL
5. Pyridoxine hydrochloride(VitaminB6)
0.05mg/mL
6. Riboflavin (VitaminB2)
0.15mg/mL
7. Folic acid (VitaminB9)
0.05mg/mL
8. Thiamine hydrochloride
0.15mg/mL
F005
Separation of Fat-soluble Vitamins
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
Acetonitrile/ Methanol = 60/40
Flow Rate
1.0mL/min.
Detection
UV280nm
Temperature
40ºC
Sample Vol.
5μL
Samples
06 FOOD
0.16AUFS
1. Menadione (Vitamin K3)
0.25mg/mL
2. Retinol acetate (Vitamin A acetate)
0.09mg/mL
3. Ergocalciferol (Vitamin D2)
0.10mg/mL
4. Cholecalciferol (Vitamin D3)
0.10mg/mL
5. Tocopherol (Vitamin E)
0.75mg/mL
6. Tocopherol acetate (Vitamin E acetate)
1.00mg/mL
7. Phylloquinone (Vitamin K1)
0.50mg/mL
F070
F003
Separation of Vitamin C
Separation of Vitamin D2, D3
Column
Mightysil RP-18 250-4.6mmØ 5μm
Column
Mightysil RP-18GP Aqua 150-4.6mmØ 5μm
Eluent
Acetonitrile/ Water = 95/ 5
Eluent
1% Methaphosphoric acid
Flow Rate
1.0mL/min.
Flow Rate
1.0mL/min.
Detection
UV210nm
Detection
UV242nm
Temperature
Room Temperature
Temperature
Ambient
Sample Vol.
5μL
Sample Vol.
5μL
Samples
Samples
1. L-Ascorbic acid
50mg/mL
0.08AUFS
1. Ergocalciferol (Vitamin D2)
250μg/mL
2. Cholecalciferol (Vitamin D3)
250μg/mL
F057
Separation of Tocopherols
Column
Mightysil Si60 150-4.6mmØ 5μm
Eluent
Hexane/ Dioxane/ Isopropylalcohol = 97.5/ 2/ 0.5
Flow Rate
1.0mL/min.
Detection
Fluorescence 323nm (Excitation 296nm)
Temperature
Ambient
Sample Vol.
5μL
Samples
1. α-Tocopherol
1μg/mL
2. 2,2,5,7,8-Pentamethylhydroxychroman
1μg/mL
3. β-Tocopherol
1μg/mL
4. γ-Tocopherol
1μg/mL
5. δ-Tocopherol
1μg/mL
FOOD 07
F058
F097
Separation of Vitamin C and E
Column
Mightysil RP-18GP 250-4.6mmØ 5μm
Eluent
A: Methanol/ 20mM Phosphoric acid = 1/ 99
Separation of Vitamin B12 in Health food
B : Methanol/ Acetonitrile = 40/60
Gradient, Flow rate :
0~10min.:
A100%, 1.0mL/min
Column
Mightysil RP-18GP Aqua 150-4.6mmØ 5μm
10.1~30min.: B100%, 1.5mL/min
Eluent
Acetonitrile / 50mM Ammonium acetate = 10/90
Detection
UV254nm
Flow Rate
1.0mL/min.
Temperature
40°C
Detection
VIS550nm
Sample Vol.
10μL
Temperature
40°C
Samples
1. L-Ascorbic acid (Vitamin C)
0.2mg/mL
Sample Vol.
20μL
2. Tocopherol (Vitamin E)
0.2mg/mL
Samples
Cyanocobalamin(Vitamin B12)
F071
Separation of Carotenoids
Column
Mightysil RP-18(L)GP 150-4.6mmØ 5μm
Eluent
Acetonitrile/ Methanol = 50/50
Flow rate
1.0mL/min.
Detection
UV460nm
Temperature
40°C
Sample Vol.
10μL
Samples
1. Lutein
2. β-Cryptoxanthin
08 FOOD
4mg/mL
2mg/mL
3. Lycopene
20mg/mL
4. β-Carotene
20mg/mL
F004
F059
Separation of β-Carotene in Broccoli
Separation of β-Carotene
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
Chloroform/ Methanol = 20/80
Column
Mightysil RP-18GP 250-4.6mmØ 5μm
Flow Rate
1.0mL/min.
Eluent
Ethanol 100%
Detection
453nm
Flow Rate
1.0mL/min.
Temperature
Room Temperature
Detection
UV-VIS 453nm
Sample Vol.
10μL
Temperature
40°C
Sample
1. β-Carotene
Sample Vol.
10μL
Samples
1. β-Carotene
0.005AUFS
Standard: 0.5μg/mL
Actual: Extraction in acetone
2μg/mL
F007
Separation of Synthetic Antibiotics (Quinolone Derivatives) - 1
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 50mM Sodium dihydrogenphosphate buffer (pH2.5*)
= 35/ 65, including 3.5mM of Sodium dodecyl sulfate
[*pH adjust : H3PO4]
Flow Rate
0.6mL/min.
Detection
UV320nm
Temperature
25°C
Sample Vol.
10μL (50ng)
Samples
1. Oxolinic acid
2. Nalidixic acid
3. Flumequine
4. Ofloxacin
5. Benofloxacin
6. Danofloxacin
7. Piromidic acid
8. Enrofloxacin
0.04AUFS
9. Orbifloxacin
Each 5μg/mL
FOOD 09
F008
Separation of Synthetic Antibiotics (Quinolone Derivatives) - 2
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 50mM Sodium dihydrogenphosphate buffer (pH 2.4*)
including 2.5mM of Sodium heptanesulfonate = 20/ 80
[*pH adjust : conc.H3PO4]
Flow Rate
0.6mL/min.
Detection
Fluorescence 445nm ( Excitation 295nm)
Temperature
40°C
Sample Vol.
5μL
Samples
1. Danofloxacin mesylate
2. Enrofloxacin
F009
Separation of Synthetic Antibiotics (Sulfa drugs)
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 25mM Sodium dihydrogenphosphate= 25/75
Flow Rate
0.5mL/min.
Detection
UV265nm
Temperature
40°C
Sample Vol.
5μL
Samples
10 FOOD
0.04AUFS
1. Sulfisomidine
5μg/mL
3. Sulfamerazine
10μg/mL
2. Sulfathiazole
10μg/mL
4. Sulfamethoxypyridazine 10μg/mL
5. Sulfamonomethoxine 10μg/mL
6. Sulfachlorpyridazine
10μg/mL
7. Sulfamethoxazole 10μg/mL
8. Sulfadimethoxine
10μg/mL
F010
F011
Separation of Synthetic Antibiotics (Furan Derivatives)
Separation of Synthetic Antibiotics (Protozoicides)
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 25mM Sodium dihydrogenphosphate= 50/50
Column
Mightysil RP-18 150-4.6mmØ 5μm
Flow Rate
0.5mL/min.
Eluent
Acetonitrile/ 25mM Sodium dihydrogenphosphate= 35/65
Detection
UV265nm
Flow Rate
0.5mL/min.
Temperature
40°C
Detection
UV265nm
Sample Vol.
5μL
Temperature
40°C
Samples
1. Amprolium
Sample Vol.
5μL
Samples
1. Nitro furazone
10μg/mL
3. Ethopabate
5μg/mL
2. Furazolidone
10μg/mL
4. Nicarbazin
65μg/mL
0.04AUFS
0.04AUFS
2. Pyrimethamine
15μg/mL
5μg/mL
F012
Separation of Synthetic Antibiotics (Protozoicide & Quinolone)
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 10mM Potassium dihydrogenphosphate including
5mM of Sodium heptanesulfonate = 20/80
Flow Rate
1.0mL/min.
Detection
UV265nm
Temperature
40°C
Sample Vol.
5μL
Samples
1. Amprolium
0.04AUFS
2. Oxolinic acid
13.75μg/mL
3.75μg/mL
FOOD 11
F013
Separation of 18 Synthetic Antibiotics
Column
Mightysil RP-18 250-4.6mmØ 5μm
Eluent
Acetonitrile/ 25mM Potassium dihydrogenphosphate
= 15/ 85 (Eluent A), = 70/30 (Eluent B)
Flow Rate
0.5mL/min.
Detection
UV265nm
Temperature
40°C
Sample Vol.
10μL
Samples
ODX: Olaquindox
1μg/mL each
SMR: Sulfamerazine SMM: Sulfamonomethoxine PYR: Pyrimethamine
0.04AUFS
OMP: Ormethoprim
OXA: Oxolinic acid
CLP: Clopidol
CDX: Carbadox
DFZ: Difurazone
FZD: Furazolidone
MRT: Morantel citrate
NAA: Nalidixic acid
TMP: Trimethoprim
SDM: Sulfadimethoxine
PMA: Piromidic acid
SDD: Sulfadimidine
SQX: Sulfaquinoxaline
NCZ: Nicarbazin
Separation of 18 Synthetic Antibiotics
12 FOOD
F026
F027
Separation of Carbadox
Separation of a Metabolite of Carbadox
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 10mM Potassium dihydrogenphosphate buffer
(pH2.5*)= 1/ 4 [*pH adjust : conc.H3PO4]
1.0mL/min.
Flow Rate
0.3mL/min.
UV310nm
Detection
UV320nm
Temperature
40°C
Temperature
40°C
Sample Vol.
2μL
Sample Vol.
5μL
Sample
1. Carbadox
Sample
1. 2-Quinoxalinecarboxylic acid
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 25mM Potassium dihydrogenphosphate= 1/9
Flow Rate
Detection
10μg/mL
F028
50μg/mL
F006
Separation of Sulfadimidine
Separation of Tetracycline Antibiotics
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 50mM Phosphate buffer(pH2.5) = 25/75
Flow Rate
1.0mL/min.
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Detection
UV350nm
Eluent
Acetonitrile/ 25mM Sodium dihydrogenphosphate = 3/17
Temperature
25°C
Flow Rate
1.0mL/min.
Sample Vol.
10μL (25ng)
Detection
UV268nm
Samples
1. Oxytetracycline
Temperature
40°C
Sample Vol.
20μL
Sample
1. Sulfadimidine
0.04AUFS
2. Tetracycline
3. Chlortetracycline
0.5μg/mL
4. Doxycycline
FOOD 13
F014
Separation of Drugs for Animals – 1
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
A: Methanol/ 25mM of potassium dihydrogenphosphate = 20/80
B: Methanol/ 25mM of potassium dihydrogenphosphate = 95/5
Linear gradient from 100% of A to 100% of B for 25 min.
Flow Rate
1.0mL/min.
Detection
UV254nm
Temperature
40°C
Sample Vol.
20μL
Samples
1. Oxytetracycline
50μg/mL
2. Flubendazole
5μg/mL
3. β-Trenbolone
20μg/mL
4. α-Trenbolone
20μg/mL
5. Zeranol
20μg/mL
6. Closantel
10μg/mL
7. Ivermectin
20μg/mL
F015
F019
Separation of Drugs for Animals – 2
Analysis of Oxytetracycline in Pork
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
Acetonitrile/ Phosphate buffer pH3.0 = 15/85
Flow Rate
0.5mL/min.
Detection
UV285nm
Column
Mightysil RP-18 150-6.0mmØ 5μm
Temperature
40°C
Eluent
1M of Imidazole buffer(pH7.2)/ Methanol = 4/1
Sample Vol.
5μL
Flow Rate
1.0mL/min.
Samples
1. 5-Hydroxythiabendazole
5μg/mL
Detection
Fluorescence 520nm (Excitation 380nm)
2. 5-Propylsulfonyl-1H-benzimidazole-2-amine
5μg/mL
Temperature
40°C
15μg/mL
Sample Vol.
20μL
Sample
0.1ppm of Oxytetracycline was added to pork
0.03AUFS
3. 2-Quinoxalinecarboxylic acid
4. Sulfadimidine
14 FOOD
7μg/mL
F016
F017
Analysis of α-Trenbolone in Pork
Analysis of β-Trenbolone in Pork
Column
Mightysil RP-18 150-4.6mmØ 5μm
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
Acetonitrile/ Water = 5/6
Eluent
Acetonitrile/ Water = 5/6
Flow Rate
0.5mL/min.
Flow Rate
0.5mL/min.
Detection
UV340nm
Detection
UV340nm
Temperature
40°C
Temperature
40°C
Sample Vol.
100μL
Sample Vol.
100μL
Sample
0.01ppm of α-Trenbolone was added to pork
Sample
0.02ppm of β-Trenbolone was added to pork
F021
F018
Analysis of Zeranol in Pork
Analysis of Ivermectin in Pork
Column
Mightysil RP-18 150-4.6mmØ 5mm
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
Acetonitrile/ Water = 5/6
Eluent
Methanol/ Water = 95/5
Flow Rate
0.5mL/min.
Flow Rate
1.5mL/min.
Detection
UV230nm
Detection
Fluorescence 425nm (Excitation 380nm)
Temperature
40°C
Temperature
40°C
Sample Vol.
100μL
Sample Vol.
20μL
Sample
Actual pork (muscles)
Sample
0.02ppm of Ivermectin was added to pork
FOOD 15
F020
F022
Analysis of Closantel in Pork
Analysis of Flubendazole in Pork
Column
Mightysil RP-18 150-6.0mmØ 5μm
Eluent
Methanol/ 20mM Sodium dihydrogenphosphate buffer(pH3.3*) =
7/ 2 [*pH adjust : H3PO4]
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
Acetonitrile/ Water = 7/13
Flow Rate
1.5mL/min.
Flow Rate
1.0mL/min.
Detection
UV369nm
Detection
UV313nm
Temperature
50°C
Temperature
40°C
Sample Vol.
20μL
Sample Vol.
20μL
Sample
1ppm of Closantel was added to pork
Sample
0.01ppm of Flubendazole was added to pork
F029
F030
Separation of Thiabendazole
Separation of a Metabolite of Thiabendazole
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 25mM Sodium dihydrogenphosphate = 1/9
Eluent
Acetonitrile/ 25mM Sodium dihydrogenphosphate = 1/9
Flow Rate
1.5mL/min.
Flow Rate
0.5mL/min.
Detection
Fluorescence 370nm (Excitation 300nm)
Detection
UV298nm
Temperature
40°C
Temperature
40°C
Sample Vol.
1μL
Sample Vol.
100μL
Sample
1. Thiabendazole
Sample
1. 5-Hydroxythiabendazole
16 FOOD
0.25μg/mL
0.05μg/mL
F023
F024
Separation of Albendazole
Separation of a Metabolite of Albendazole
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 25mM Sodium dihydrogenphosphate = 4/6
Eluent
Acetonitrile/ 25mM Sodium dihydrogenphosphate = 3/17
Flow Rate
1.0mL/min.
Flow Rate
1.0mL/min.
Detection
UV220nm
Detection
Fluorescence 320nm (Excitation 280nm) 0.14AUFS
Temperature
40°C
Temperature
40°C
Sample Vol.
20μL
Sample Vol.
1μL
Sample
1. Albendazole
Sample
1. 5-Propylsulfonyl-1H-benzimidazole-2-amine
0.35AUFS
0.01mg/mL
F025
0.5μg/mL
F031
Separation of Isomethamidium
Separation of Avoparcins
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Column
Mightysil RP-18GP 250-4.6mmØ 5μm
Eluent
Acetonitrile/ 30mM Citric acid including 5mM of Sodium
heptanesulfonic acid = 3/7
Eluent
Acetonitrile/ 2.5% Acetate buffer(pH4.0*) including 10mM of
Sodium heptanesulfonate = 11.5/ 88.5 [*pH adjust : NH4OH]
Flow Rate
1.0mL/min.
Flow Rate
1.0mL/min.
Detection
UV380nm
Detection
UV280nm
Temperature
40°C
Temperature
Room Temperature (22°C)
Sample Vol.
15μL
Sample Vol.
20μL
Sample
1. Isometamidium
Sample
Control material including α-Avoparcin and β-Avoparcin
0.1μg/mL
FOOD 17
F044
F047
Separation of Preservatives - 1
Separation of Presevatives -2
Column
Mightysil RP-18 150-4.6mmØ 5μm
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 20mM Phosphate buffer(pH4.6) = 1/2
Eluent
Acetonitrile/ 20mM Buffers pH4.6 = 1/2
Flow Rate
0.5mL/min.
Flow Rate
0.5mL/min.
Detection
UV225nm
Detection
UV225nm
Temperature
Room Temperature
Temperature
Room Temperature
Sample Vol.
20μL
Sample Vol.
20μL
Samples
1. Benzoic acid
20μg/mL
Samples
1. Benzoic acid
20μg/mL
2. Sorbic acid
20μg/mL
2. Sorbic acid
20μg/mL
3. Dehydro acetic acid
20μg/mL
3. Dehydroacetic acid
20μg/mL
1.28AUFS
F075
0.32AUFS
F043
Separation of Presevatives - 3
Separation of Preservatives - 4
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
5mM Citric acid buffer*/ Acetonitrile/ Methanol = 70/ 20/10
[*0.7g/L Citric acid monohydrate + 0.6g/L Trisodium citrate
dehydrate]
Eluent
Methanol/ Water = 1/1
Flow Rate
1.0mL/min.
Detection
UV254nm
Flow Rate
1.0mL/min.
Temperature
40°C
Detection
UV230nm
Sample Vol.
10μL
Temperature
40°C
Samples
1. Methyl p-hydroxybenzoate
1μg/mL
Sample Vol.
10μL
2. Ethyl p-hydroxybenzoate
1μg/mL
Samples
1. Benzoic acid
100μg/mL
3. Isopropyl p-hydroxybenzoate
1μg/mL
2. Sorbic acid
100μg/mL
4. n-Propyl p-hydroxybenzoate
1μg/mL
3. Salicylic acid
100μg/mL
5. Isobutyl p-hydroxybenzoate
1μg/mL
4. Dehydroacetic acid
100μg/mL
6. n-Butyl p-hydroxybenzoate
1μg/mL
18 FOOD
F074
Separation of Preservatives - 5
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
Methanol/ 5mM Citric acid buffer* = 50/50
[*0.7g/L Citric acid monohydrate + 0.6g/L Trisodium citrate
dihydrate]
Flow Rate
1.0mL/min.
Detection
UV270nm
Temperature
40°C
Sample Vol.
20μL
Samples
1. Methyl p-hydroxybenzoate
4.0μg/mL
2. Ethyl p-hydroxybenzoate
4.0μg/mL
3. Isopropyl p-hydroxybenzoate
4.0μg/mL
4. Propyl p-hydroxybenzoate
4.0μg/mL
5. Isobutyl p-hydroxybenzoate
4.0μg/mL
6. Butyl p-hydroxybenzoate
4.0μg/mL
F077
Separation of Presevatives - 6
Column
Mightysil RP-8GP 150-4.6mmØ 5μm
Eluent
Ethanol/ 50mM Sodium dihydrogen phosphate dihydrate = 34/
66, including 2mM of Cetyltrimethylammonium bromide (pH4.41*)
[*pH adjust : 10% Phosphoric acid, after mixing the components of
eluent.]
Flow Rate
1.0mL/min.
Detection
UV240nm
Temperature
45°C
Sample Vol.
5μL
Samples
1. Ethyl p-hydroxybenzoate
0.1mg/mL
2. Sorbic acid
0.1mg/mL
3. Benzoic acid
0.1mg/mL
4. Isopropyl p-hydroxybenzoate
0.1mg/mL
5. Propyl p-hydroxybenzoate
0.1mg/mL
6. Saccharin
0.1mg/mL
7. Isobutyl p-hydroxybenzoate
0.1mg/mL
8. Butyl p-hydroxybenzoate
0.1mg/mL
0.32AUFS
FOOD 19
F061
F042
Separation of Preservatives and Saccharin
Separation of Antioxidants
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
0.1% Pottasium phosphate buffer*/ Acetonitrile = 95/5
[*0.1% Pottasium dihydrogen phosphate / 0.1% Dipotassium
hydrogen phosphate = 1/1]
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
Methanol/ Water = 8/2
Flow Rate
0.7mL/min.
Flow Rate
1.0mL/min.
Detection
UV225nm
Detection
UV280nm
Temperature
40°C
Temperature
Room Temperature
Sample Vol.
10μL
Sample Vol.
10μL
Samples
1. Benzoic acid
10μg/mL
Samples
1. Butylhydroquinone (BHQ)
100μg/mL
2. Sorbic acid
10μg/mL
2. Butylhydroxyanisole (BHA)
100μg/mL
3. Saccharin
10μg/mL
3. Butyl hydroxytoluene (BHT)
100μg/mL
F073
Separation of Antioxdants - 2
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
A: Acetonitrile/ Methanol = 50/50
B: 0.5% Acetic acid
Gradient (A%) :0~25min. : A45%~100% Linear,
25.1~30min.: A 100%, 30.1~50min.: A45%
Flow Rate
1.0mL/min.
Detection
UV280nm
Temperature
40°C
Sample Vol.
10μL
Samples
1. Propyl gallate (PG)
40μg/mL
2. Trihydroxybutyrophenone (THBP)
40μg/mL
3. Butyl hydroquinone (BHQ)
40μg/mL
4. Nordihydroguaiaretic acid (NDGA)
40μg/mL
5. Butyl hydroxyanisole (BHA)
40μg/mL
6. Hydroxy methyl butyl phenol (HMBP)
40μg/mL
7. Octyl gallate (OG)
40μg/mL
8. Butyl hydroxytoluene (BHT)
40μg/mL
9. Dodecyl gallate (DG)
40μg/mL
20 FOOD
0.16AUFS
F048
F045
Separation of Ascorbic Acid & Erythorbic Acid
Separation of anti-Fungal Preservatives - 1
Column
Mightysil RP-18 150-4.6mmØ 5μm
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
Methanol/ Water = 75/25
Eluent
50mM Disodium hydrogen phosphate buffer(pH6.8*) including
5mM of Tetrabutylammonium hydrogensulfate
Flow Rate
1.0mL/min.
Detection
UV230nm
Flow Rate
1.0mL/min.
Temperature
40°C
Detection
UV254nm
Sample Vol.
3μL
Temperature
40°C
Samples
1. Thiabendazole (TBZ)
Sample Vol.
1μL
Samples
1. Ascorbic acid
2. Erythorbic acid
0.16AUFS
160μg/mL
2. o-Phenylphenol (OPP)
40μg/mL
1mg/mL
3. Imazalil (IMZ)
70μg/mL
1mg/mL
4. Biphenyl (BP)
50μg/mL
F046
F037
Separation of anti-Fungal Preservatives - 2
Separation of Food Dyes - 1
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 0.05% Potassium phosphate buffer* = 6/4
[*0.05% Potassium dihydrogen phosphate/0.05% Dipotassium
hydrogen phosphate = 1/1]
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
A: Methanol/ 10mM Ammonium Dihydrogenphosphate = 1/9
B: Methanol/ 10mM Ammonium Dihydrogenphosphate = 8/2
Linear gradient from 100% of A to 100% of B for 25min.
Flow Rate
0.65mL/min.
Detection
Fluorescence 325nm (Excitation 280nm)
Flow Rate
1.0mL/min.
Temperature
40°C
Detection
UV254nm
Sample Vol.
5μL
Temperature
Room Temperature
Samples
1. Thiabendazole (TBZ)
1μg/mL
Sample Vol.
10μL
2. o-Phenylphenol (OPP)
1μg/mL
Samples
1. Tartrazine (Yellow-4)
40μg/mL
3. Biphenyl (BP)
1μg/mL
2. Sunset Yellow FCF (Yellow-5)
60μg/mL
0.16AUFS
FOOD 21
F038
Separation of Food Dyes - 2
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
A: Methanol/ 10mM Ammonium Dihydrogenphosphate = 1/9
B: Methanol/ 10mM Ammonium Dihydrogenphosphate = 8/2
Linear gradient from 100% of A to 100% of B for 25min.
Flow Rate
1.0mL/min.
Detection
UV254nm
Temperature
Room Temperature
Sample Vol.
20μL
Samples
1. Indigocarmine (Blue-2)
40μg/mL
2. Fast Green FCF (Green-3)
30μg/mL
3. Brilliant Blue FCF (Blue-1)
30μg/mL
0.16AUFS (A), UV612nm
0.64AUFS (B)
F039
Separation of Food Dyes - 3
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
A: Methanol/ 10mM Ammonium Dihydrogenphosphate = 1/9
B: Methanol/ 10mM Ammonium Dihydrogenphosphate = 8/2
Linear gradient from 100% of A to 100% of B for 25min., then
keeping 100% of B.
Flow Rate
1.0mL/min.
Detection
UV254nm
Temperature
Room Temperature
Sample Vol.
10μL
Samples
22 FOOD
0.16AUFS
1. Amaranth (R2)
40μg/mL
2. New Coccine (R102) 40μg/mL
3. Acid Red (R106)
40μg/mL
4. Eosin Y
40μg/mL
5. Erythrosine (R3)
40μg/mL
6. Phloxine (R104)
40μg/mL
7. Rose Bengal (R105)
60μg/mL
F040
Separation of Food Dyes - 4
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
A: Methanol/ 10mM Ammonium Dihydrogenphosphate = 1/9
B: Methanol/ 10mM Ammonium Dihydrogenphosphate = 8/2
Linear gradient from 100% of A to 100% of B for 25min., then
keeping 100% of B.
Flow Rate
1.0mL/min.
Detection
UV254nm
Temperature
Room Temperature
Sample Vol.
20μL
Samples
0.16AUFS
1. Tartrazine (Y4)
20μg/mL
2. Amaranth (R2)
3. Indigocarmine (B2)
30μg/mL
4. New Coccine (R102) 20μg/mL
5. Sunset Yellow FCF (Y5)
30μg/mL
6. Fast Green FCF (G3) 50μg/mL
7. Brilliant Blue FCF (B1)
50μg/mL
8. Acid Red (R106)
20μg/mL
9. Eosin Y
20μg/mL
10. Erythrosine (R3)
20μg/mL
50μg/mL
12. Rose Bengal (R105) 30μg/mL
11. Phloxine (R104)
20μg/mL
F041
Separation of Food Dyes - 5
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
A: Methanol/ 10mM Ammonium Dihydrogenphosphate = 1/9
B: Methanol/ 10mM Ammonium Dihydrogenphosphate = 8/2
Linear gradient from 100% of A to 100% of B for 25min.
Flow Rate
1.0mL/min.
Detection
UV428nm (0-10min.); UV630nm (10-20min.);
UV254nm (20-30min.) 0.08AUFS
Temperature
Room Temperature
Sample Vol.
20μL
Samples
1. Tartrazine Aluminium Lake
50μg/mL
2. Brilliant Blue Aluminium Lake
50μg/mL
3. Erythrosine Aluminium Lake
50μg/mL
FOOD 23
F072
Separation of Food Dyes - 6
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
A: Methanol/ Acetonitrile/ Water = 3/3/4 Containing Ammonium
acetate solution(5g/100mL)
B: Ammonium acetate solution(5g/100mL)
Gradient : 0~30min. : A5%-A100% Linear,
30~36min.: A100%, 36.1~50min.: A5%
Flow Rate
1.4mL/min.
Detection
UV254nm
Temperature
40°C
Sample Vol.
20μL
Samples
1. Tartrazine (Y4)
2. Amaranth (R2)
3. Indigocarmine (B2)
4. New Coccine (R102)
5. Sunset Yellow FCF (Y5)
6. Allura Red AC (R40)
7. Fast Green FCF (G3)
9. Eosin
8. Brilliant Blue FCF (B1)
10. Erythrosine (R3)
11. Acid Red (R106)
12. Phloxine (R104)
13. Rose Bengal (R105)
(Each Sample: 20μg/mL)
F060
F050
Separation of Anthocyanins
Separation of Aspartame in a Beverage
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
Methanol/ 33mM Phosphate buffer(pH4.9*) = 3/7
[*pH adjust : H3PO4]
Column
Mightysil RP-18GP 250-4.6mmØ 5μm
Eluent
A : 0.5% Trifluoroacetic acid
B : Trifluoroacetic acid/ Acetonitrile = 50/50
Flow Rate
1.0mL/min.
Detection
UV210nm
Flow Rate
1.0mL/min.
Temperature
Room Temperature
Detection
UV-VIS 520nm
Sample Vol.
2μL
Temperature
40°C
Sample
Actual beverage:
Sample Vol.
10μL
Samples
1. Acylanthocyanin
1mg/mL
2. Caffeine
2. Anthocyanin
3mg/mL
3. Aspartame
24 FOOD
0.16AUFS
1. Benzoic acid
F069
F080
Separation of Acesulfame K
Separation of Aspartame and Acesulfame K in breath mint
Column
Mightysil RP-18 GP 150-4.6mmØ 5μm
Eluent
Methanol/ 10mM Potassium dihydrogenphosphate(pH4.0*)
including 10mM of Tetra-n-propylammonium hydroxide = 25/75
[*pH adjust : H3PO4]
Flow Rate
1.0mL/min.
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Detection
DAD (200 〜 400nm)
Eluent
Acetonitrile/ 10mM Tetrabutylammonium
dihydrogenphosphate = 40/60
Temperature
40°C
Sample Vol.
10μL
Flow Rate
1.0mL/min.
Samples
1. Aspartame
Detection
UV227nm
Temperature
40°C
Sample Vol.
20μL
Samples
1. Acesulfame K
0.01mg/mL
2. Ethyl-p-hydroxybenzoate
0.01mg/mL
2. Acesulfame K
Each 0.1mg/mL in Water
Commercial breath mint:
F049
5 tablets of breath mint dissolved to 10mL of Water and filtered
with 0.2μm PVDF membrane.
F076
Separation of Saccharin & Sorbic Acid
Separation of Cyclamic acid
Column
Mightysil RP-18GP 250-4.6mmØ 5μm
Eluent
Acetonitrile/ 0.1% Potassium phosphate buffer* = 5/95
[*0.1% Potassium dihydrogen phosphate/ 0.1% Dipotassium
hydrogen phosphate = 1/1 ]
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Flow Rate
0.8mL/min.
Eluent
Methanol/ Water = 80/20
Detection
UV230nm
Flow Rate
1.0mL/min.
Temperature
40°C
Detection
UV314nm
Sample Vol.
10μL
Temperature
40°C
Samples
1. Saccharin
10μg/mL
Sample Vol.
20μL
2. Sorbic acid
10μg/mL
Samples
1. Cyclamic acid
20μg/mL
FOOD 25
F051
F089
Separation of Pyrazines
Separation of Ubiquinone 9, 10
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 10mM Potassium dihydrogenphosphate = 3/7
Flow Rate
0.15mL/min.
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Detection
UV273nm
Eluent
Methanol/ Ethanol = 13/7
Temperature
Room Temperature
Flow Rate
2.0mL/min.
Sample Vol.
5μL
Detection
UV275nm
Samples
1. Pyrazine
Temperature
35°C
2. 2-Methylpyrazine
Sample Vol.
5μL
3. 2,5-Dimethylpyrazine
Samples
1. Ubiquinone 9 (Coenzyme Q9)
0.32AUFS
4. 2,3,5-Trimethylpyrazine
2. Ubiquinone 10 (Coenzyme Q10)
5. 2,3,5,6-Tetramethylpyrazine
Total of 500ppm
F090
F091
Separation of DL-Thioctic acid
Separation of Benzoyl Peroxide in Flour
Column
Mightysil RP-18GP 250-4.6mmØ 5μm
Eluent
Methanol/ Acetonitrile/ 0.005mol/L Phosphate Buffer
(pH3.08*)=1160/180/920[*pH adjust : dil.H3PO4]
Column
Mightysil RP-18GP 250-4.6mmØ　5μm
Flow Rate
1.2mL/min.
Eluent
Acetonitrile / Water = 55/45
Detection
UV215nm
Flow Rate
1.0mL/min.
Temperature
35°C
Detection
UV235nm
Sample Vol.
20μL
Temperature
40°C
Samples
DL-Thioctic acid 1mg/mL
Sample Vol.
20μL
( 0.005mol/L Phosphate buffer pH3.5/Acetonitrile=1/1.)
Preparation
The Acetonitrile is added to flour , and filtered
26 FOOD
F053
Separation of Caffeine and Catechins
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
A: 0.1% Phosphoric acid including 5% of
N,N-Dimethylformamide and 0.1% of Acetonitrile
B: Acetonitrile
Flow Rate
1.0mL/min.
Detection
UV280nm
Temperature
43°C
Sample Vol.
5μL (Standards), 3μL (Actual sample): Extracted with mixture of
Acetonitrile and Water
Samples
1. Caffeine
0.10mg/mL in STD.
2. Epigallocatechin
0.05mg/mL in STD.
3. Catechin
0.05mg/mL in STD
4. Epicatechin
0.05mg/mL in STD.
5. Epigallocatechin Gallate
0.05mg/mL in STD
6. Epicatechin Gallate
0.05mg/mL in STD.
0.08AUFS
F054
Separation of Commercial Tea drink
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
A : Acetonitrile/ Water/ Phosphoric acid = 10/ 400/ 0.2
B : Methanol/ Acetonitrile/ Water/ Phosphoric acid =
200/ 10/ 400/ 0.2
A 100% : 0~3min., A100%~A0%(B100%) : 3~25min.,
B 100% : 25~26min.
Flow Rate
1.0mL/min.
Detection
UV230nm
Temperature
40°C
Sample Vol.
10μL (Commercial green tea drink)
Samples
1. Gallic acid
2. Theogallin
3. Catechin gallate
4. Epigallocatechin
5. Catechin
6. Caffeine
7. Epigallocatechin gallate
9. Gallocatechin gallate
8. Epicatechin
10. Epicatechin gallate
11. Catechin gallate
FOOD 27
F055
Separation of Catechins
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
A: 0.1% Phosphoric acid containing 0.1% Acetonitrile and 5% N,NDimethylformamide
B : Acetonitrile
Flow Rate
1.0mL/min.
Detection
UV280nm
Temperature
40°C
Sample Vol.
5μL
Samples
1. Caffeine
2. Epigallocatechin
3. Catechin
4. Epicatechin
5. Epigallocatechin gallate
6. Gallocatechin gallate
7. Epicatechin gallate
8. Catechin gallate
F056
Separation of Catechins, Caffeine, Chlorophyll A
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
A: 1% Phosphoric acid, B : Ethanol
Time (min.) 0
0.1
A
95
90
B
5
10
17
80
20
Flow Rate
1.0mL/min.
Detection
0~21min. : UV280nm, 21~35min.: UV430nm
Temperature
40°C
Sample Vol.
5μL
Samples
1. Epigallocatechin
3. Caffeine
20
5
95
20μg/mL 2. Catechin
4μg/mL 4. Epicatechin
35
5
95
20μg/mL
20μg/mL
5. Epigallocatechin gallate 10μg/mL 6. Gallocatechin gallate 6μg/mL
28 FOOD
7. Epicatechin gallate
8μg/mL 8. Catechin gallate
9. Chlorophyll a
6μg/mL
6μg/mL
F063
F064
Separation of Allyl isothiocyanate
Separation of Quassins
Column
Mightysil RP-8GP 250-4.6mmØ 5μm
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
Acetonitrile/ Water = 70/30
Eluent
Acetonitrile/ Water = 35/75
Flow Rate
0.8 mL/min.
Flow Rate
1.0mL/min.
Detection
UV240nm
Detection
UV254nm
Temperature
40°C
Temperature
30°C
Sample Vol.
10μL
Sample Vol.
10μL
Samples
1. Allyl isothiocyanate
0.4mg/mL
Samples
1.2. : Quassin
100μg/mL
2. Fenethylcyanate
0.3mg/mL
3. p-Methylacetophenon
12.5μg/mL
F065
F082
Separation of Capsaicins
Separation of Curcumin
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
Acetonitrile/20mM Formic acid = 45/55
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Flow rate
1.0mL/min.
Eluent
Acetonitrile/ Water/ Acetic acid = 43/ 57/ 1
Detection
UV425nm
Flow Rate
1.2 mL/min.
Temperature
40°C
Detection
UV280nm
Sample Vol.
5μL
Temperature
45°C
Samples
Curcumin
Sample Vol.
15μL
Samples
1. Capsaicin
2. Dihydrocapsaicin
0.1mg/mL
Commercial drink
160μg/mL
80μg/mL
The sample solution filtered with PVDF filter, after having diluted
the commercial drink to 3 times with Acetonitrile.
FOOD 29
F083
F078
Separation of Curcumin in a commercial drink by LC/MS
Separation of Rutin and Quercetin
Column
Mightysil RP-18GP 150-2.0mmØ 5μm
Eluent
Acetonitrile/20mM Formic acid = 45/55
Column
Mightysil RP-18GP 250-4.6mmØ 5μm
Flow Rate
0.2mL/min.
Eluent
2.5% Acetic acid/ Methanol/ Acetonitorile = 70/ 10/ 20
Detection
MS ESI-
Flow Rate
1.0mL/min.
Temperature
40°C
Detection
UV350nm
Sample Vol.
5μL
Temperature
30°C
Samples
Commercial drink
Sample Vol.
10μL
The sample solution filtered with PVDF filter, after having diluted
the commercial drink to 3 times with Acetonitrile.
Samples
1. Rutin
0.8mg/mL
2. Quercetin
0.4mg/mL
F099
F100
Separation of Apigenin
Separation of 5,6-Benzoflavone
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 25mM Potassium dihydrogenphosphate buffer(pH2.4*)
= 30/70 [*pH adjust : H3PO4]
Eluent
Acetonitrile/ 25mM Potassium dihydrogenphosphate buffer(pH2.4*)
= 60/40 [*pH adjust : H3PO4]
Flow rate
1.0mL/min.
Flow Rate
1.0mL/min.
Detection
UV340nm
Detection
UV270nm
Temperature
40ºC
Temperature
40ºC
Sample Vol.
50μL
Sample Vol.
10μL
Samples
1. Apigenin
Sample
1. 5,6-Benzoflavone
30 FOOD
0.02mg/mL
0.2mg/mL
F101
F102
Separation of 7,8-Benzoflavone
Separation of Biochanin A
Columns
Mightysil RP-18GP 150-4.6mmØ 5μm
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 25mM Potassium dihydrogenphosphate buffer(pH2.4*)
= 55/45 [*pH adjust : H3PO4]
Eluent
Acetonitrile/ 25mM Potassium dihydrogenphosphate buffer(pH2.4*)
= 50/50 [*pH adjust : H3PO4]
Flow Rate
1.0mL/min.
Flow Rate
1.0mL/min.
Detection
UV280nm
Detection
UV260nm
Temperature
40ºC
Temperature
40ºC
Sample Vol.
10μL
Sample Vol.
5μL
Sample
1. 7,8-Benzoflavone
Sample
1. Biochanin A
0.2mg/mL
F103
0.5mg/mL
F104
Separation of Chrysin
Separation of Diosmin
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 25mM Potassium dihydrogenphosphate buffer(pH2.4*)
= 40/60 [*pH adjust : H3PO4]
Eluent
Acetonitrile/ 25mM Potassium dihydrogenphosphate buffer(pH7.0*)
= 20/80 [*pH adjust : H3PO4]
Flow Rate
1.0mL/min.
Flow Rate
1.0mL/min.
Detection
UV265nm
Detection
UV254nm
Temperature
40ºC
Temperature
40ºC
Sample Vol.
5μL
Sample Vol.
5μL
Sample
1. Chrysin
Sample
1. Diosmin
0.1mg/mL
0.1mg/mL
FOOD 31
F105
F106
Separation of Flavonol
Separation of Hesperetin
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 25mM Potassium dihydrogenphosphate buffer(pH7.0*)
= 50/50 [*pH adjust : H3PO4]
Eluent
Acetonitrile/ 25mM Potassium dihydrogenphosphate buffer(pH7.0*)
= 30/70 [*pH adjust : H3PO4]
Flow Rate
1.0mL/min.
Flow Rate
1.0mL/min.
Detection
UV240nm
Detection
UV285nm
Temperature
40ºC
Temperature
40ºC
Sample Vol.
10μL
Sample Vol.
5μL
Sample
1. Flavonol
Sample
1. Hesperetin
0.2mg/mL
F107
0.5mg/mL
F108
Separation of Hesperidin
Separation of Isoquercitrin
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 25mM Potassium dihydrogenphosphate buffer(pH7.0*)
= 20/80 [*pH adjust : H3PO4]
Eluent
Acetonitrile/ 25mM Potassium dihydrogenphosphate buffer(pH7.0*)
= 20/80 [*pH adjust : H3PO4]
Flow Rate
1.0mL/min.
Flow Rate
1.0mL/min.
Detection
UV285nm
Detection
UV254nm
Temperature
40ºC
Temperature
40ºC
Sample Vol.
10μL
Sample Vol.
5μL
Sample
1. Hesperidin
Sample
1. Isoquercitrin
32 FOOD
0.1mg/mL
0.5mg/mL
F109
F110
Separation of Kaempferol
Separation of Luteolin
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 25mM Potassium dihydrogenphosphate buffer(pH7.0*)
= 30/70 [*pH adjust : H3PO4]
Eluent
Acetonitrile/ 25mM Potassium dihydrogenphosphate buffer(pH7.0*)
= 25/75 [*pH adjust : H3PO4]
Flow Rate
1.0mL/min.
Flow Rate
1.0mL/min.
Detection
UV360nm
Detection
UV345nm
Temperature
40ºC
Temperature
40ºC
Sample Vol.
20μL
Sample Vol.
20μL
Sample
1.Kaempferol
Sample
1.Luteolin
0.2mg/mL
F111
0.1mg/mL
F112
Separation of Naringenin
Separation of Naringin
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 25mM Potassium dihydrogenphosphate buffer(pH7.0*)
= 30/70 [*pH adjust : H3PO4]
Eluent
Acetonitrile/ 25mM Potassium dihydrogenphosphate buffer(pH7.0*)
= 20/80 [*pH adjust : H3PO4]
Flow Rate
1.0mL/min.
Flow Rate
1.0mL/min.
Detection
UV290nm
Detection
UV280nm
Temperature
40ºC
Temperature
40ºC
Sample Vol.
20μL
Sample Vol.
10μL
Sample
1.Naringenin
Sample
1. Naringin
0.2mg/mL
0.05mg/mL
FOOD 33
F113
F114
Separation of Rhoifolin
Separation of Trihydroxychalcone
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 25mM Potassium dihydrogenphosphate buffer(pH2.4*)
= 20/80 [*pH adjust : H3PO4]
Eluent
Acetonitrile/ 25mM Potassium dihydrogenphosphate buffer(pH2.4*)
= 35/65 [*pH adjust : H3PO4]
Flow Rate
1.0mL/min.
Flow Rate
1.0mL/min.
Detection
UV330nm
Detection
UV370nm
Temperature
40ºC
Temperature
40ºC
Sample Vol.
5μL
Sample Vol.
10μL
Sample
1. Rhoifolin
Sample
1. 4,2,4-Trihydroxychalcone
0.03mg/mL
F066
0.5mg/mL
F068
Separation of Oligosaccharide
Separation of Carbohydrates
Column
Mightysil RP-18GP Aqua 250-4.6mmØ 5μm
Eluent
100% Water
Flow rate
0.25mL/min.
Detection
RI
Temperature
35°C
Column
Mightysil RP-18GP Aqua 250-4.6mmØ 5μm
Sample Vol.
5μL
Eluent
100% Water
Samples
1. Glucose
Flow rate
0.25mL/min.
2. Maltose
Detection
RI
3. Maltotriose
Temperature
35°C
4. Maltotetraose
Sample Vol.
5μL
5. Maltopentaose
Samples
1. Glucose
1.0mg/mL
2. Sucrose
1.0mg/mL
3. Raffinose
1.0mg/mL
6. Maltohexaose
7. Maltoheptaose
34 FOOD
Each 1.0mg/mL
F032
F092
Separation of di-Carbonic Acids-1
Separation of di-Carbonic Acids-2
Column
Mightysil RP-18 150-4.6mmØ 5μm
Column
Mightysil RP-18GP Aqua 250-4.6mmØ 5μm
Eluent
Acetonitrile/ 20mM Phosphoric acid = 10/90
Eluent
Acetonitrile/20mM Phosphoric acid = 10/90
Flow Rate
1.0mL/min.
Flow Rate
1.0mL / min.
Detection
UV210nm
Detection
UV210nm
Temperature
Room Temperature
Temperature
Room Temperature
Sample Vol.
20μL
Sample Vol.
20μL
Samples
1. Malonic acid
150μg/mL
Samples
1. Malonic acid
150μg/mL
2. Succinic acid
200μg/mL
2. Succinic acid
200μg/mL
3. Glutaric acid
200μg/mL
3. Glutaric acid
200μg/mL
4. Adipic acid
200μg/mL
4. Adipic acid
200μg/mL
5. Pimelic acid
250μg/mL
5. Pimelic acid
250μg/mL
0.04AUFS
F035
0.04AUFS
F093
Separation of Organic Acids - 1
Separation of Organic Acids – 2
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
50mM Ammonium dihydrogenphosphate buffer (pH2.0*)
[*pH adjust : conc.H3PO4]
Column
Mightysil RP-18GP Aqua 250-4.6mmØ 5μm
Eluent
Ammonium dihydrogenphosphate buffer(pH2.0)
Flow Rate
1.0mL/min.
Flow Rate
1.0mL / min.
Detection
UV200nm
Detection
UV200nm
Temperature
Room Temperature
Temperature
Room Temperature
Sample Vol.
5μL
Sample Vol.
5μL
Samples
1. Oxalic acid
0.1mg/mL
Samples
1. Oxalic acid
0.1mg/mL
2. Glycolic acid
2.0mg/mL
2. Glycolic acid
2.0mg/mL
3. Malic acid
2.0mg/mL
3. Malic acid
2.0mg/mL
4. Lactic acid
3.0mg/mL
4. Lactic acid
3.0mg/mL
5. Citric acid
2.0mg/mL
5. Citric acid
2.0mg/mL
0.08AUFS
0.08AUFS
FOOD 35
F036
F094
Separation of Organic Acids - 3
Separation of Organic Acids - 4
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
50mM Ammonium dihydrogenphosphate buffer(pH2.0*)
[*pH adjust : conc.H3PO4]
Column
Mightysil RP-18GP Aqua 250-4.6mmØ 5μm
Eluent
Ammonium dihydrogenphosphate buffer(pH2.0)
Flow Rate
1.0mL/min.
Flow Rate
1.0mL / min.
Detection
UV200nm
Detection
UV200nm
Temperature
Room Temperature
Temperature
Room Temperature
Sample Vol.
5μL
Sample Vol.
5μL
Samples
1. Oxalic acid
20μg/mL
5. Maleic acid
75μg/mL
Samples
1. Oxalic acid
20μg/mL
5. Maleic acid
75μg/mL
2. Tartaric acid
300μg/mL
6. Citric acid
400μg/mL
2. Tartaric acid
300μg/mL
6. Citric acid
400μg/mL
3. Malic acid
350μg/mL
7. Fumaric acid
75μg/mL
3. Malic acid
350μg/mL
7. Fumaric acid
75μg/mL
4. Malonic acid
500μg/mL
8. Acrylic acid
75μg/mL
4. Malonic acid
500μg/mL
8. Acrylic acid
75μg/mL
0.08AUFS
F098
0.08AUFS
F062
Separation of Organic Acids -5
Separation of Formic acid and Tartaric acid
Column
Mightysil RP-18GP Aqua 150-4.6mmØ 3μm
Eluent
20mM Ammonium dihydrogenphosphate buffer(pH2.48)
Flow Rate
1.0mL/min.
Column
Mightysil RP-18GP 250-4.6mmØ 5μm
Detection
UV210nm
Eluent
Temperature
35°C
25mM Ammonium phosphate buffer (pH6.8) including 5mM of
Tetrabutylammonium hydrogen sulphate
Sample Vol.
20μL
Flow Rate
1.0mL/min.
Samples
1. Glycolic acid
100μg/mL
6. Maleic acid
Detection
UV210nm
2. Malic acid
100μg/mL
7. Citric acid
100μg/mL
Temperature
40°C
36 FOOD
1μg/mL
0.08AUFS
3. Malonic acid
100μg/mL
8. Fumaric acid
0.7μg/mL
Sample Vol.
10μL
4. Lactic acid
100μg/mL
9. Succinic acid
100μg/mL
Samples
1. Formic acid
0.7mg/mL
5. Acetic acid
100μg/mL
2. Tartaric acid
0.3mg/mL
10.Acrylic acid
1μg/mL
F033
F034
Separation of Oxalic Acid and Nitric Acid in Spinach
Column
Separation of Aromatic Acids-1
Mightysil RP-18 150-4.6mmØ 5μm
Column
Mightysil RP-18 150-4.6mmØ 5μm
5mM Tetrabutylammonium hydrogen sulfate in 50mM of
Diammonium hydrogenphosphate buffer(pH6.8*)
[*pH adjust : H3PO4]
Eluent
Acetonitrile/ 20mM phosphoric acid = 2/ 8
Flow Rate
1.0mL/min.
Detection
UV254nm
Flow Rate
1.0mL/min.
Temperature
Room Temperature
Detection
UV210nm
Sample Vol.
10μL
Temperature
Room Temperature
Samples
1. Gallic acid
Sample Vol.
20μL
2. Protocatechuic acid
Actual spinach, extracted 5g of spinach solution was diluted with
250mL of water and filtered
(1.Oxalic acid 2.Nitric acid )
3. Phthalic acid
100μg/mL
4. Gentisic acid
100μg/mL
5. Salicylic acid
200μg/mL
Eluent
Sample
0.04AUFS
F096
0.32AUFS
40μg/mL
20μg/mL
F095
Separation of Aromatic Acids -2
Separation of Aromatic Acids -3
Column
Mightysil RP-18GP Aqua 250-4.6mmØ 5μm
Column
Mightysil RP-18GP Aqua 250-4.6mmØ 5μm
Eluent
Acetonitrile/ 20mM phosphoric acid = 2/8
Eluent
Acetonitrile/ 20mM phosphoric acid = 1.5/8.5
Flow Rate
1.0mL/min.
Flow Rate
1.0mL/min.
Detection
UV254nm
Detection
UV254nm
Temperature
Room Temperature
Temperature
Room Temperature
Sample Vol.
10μL
Sample Vol.
10μL
Samples
1. Gallic acid
40μg/mL
Samples
1. Gallic acid
2. Protocatechuic acid 20μg/mL
20μg/mL
0.32AUFS
0.32AUFS
2. Protocatechuic acid 20μg/mL
40μg/mL
20μg/mL
3. Phthalic acid
100μg/mL
3. Gentisic acid
100μg/mL
4. Gentisic acid
100μg/mL
4. Phthalic acid
100μg/mL
5. Salicylic acid
200μg/mL
5. Salicylic acid
200μg/mL
FOOD 37
F084
F085
Separation of Malachite green and Leucomalachite green
Separation of Malachite green and Leucomalachite green by LC/MS
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Column
Mightysil RP-18MS 150-2.0mmØ 5μm
Eluent
A: 0.01mol/L Ammonium formate
B: Acetonitrile
Eluent
A: 0.01mol/L Ammonium formate
B: Acetonitrile
Flow Rate
2.0mL/min.
Flow Rate
0.2mL/min.
Detection
DAD: 580nm (0-13min.), 265nm (13-45min.)
Detection
MS ESI+
Temperature
40°C
Temperature
40°C
Sample Vol.
10μL
Sample Vol.
2μL
Samples
1. Malachite green
0.05mg/mL
Samples
1. Malachite green
0.05mg/mL
2. Leucomalachite green
0.05mg/mL
2. Leucomalachite green
0.05mg/mL
F086
Separation of Sudan
Column
Mightysil RP-18GP 250-4.6mmØ 5μm
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
A : Acetonitrile/ Methanol=85/15
B : Acetonitrile/ 0.1(v/v%)Formic acid=90/10
Flow Rate
1.0mL/min.
Detection
UV500nm
Temperature
40°C
Sample Vol.
10μL
Samples
1. Para red
2. Sudan Ⅰ
3. Sudan Ⅱ
4. Sudan Ⅲ
5. Sudan Ⅳ
38 FOOD
Each 5μg/mL
F087
Separation of Sudan by LC/MS
Column
Mightysil RP-18MS 150-2.0mmØ 5μm
Eluent
Acetonitrile/ 0.1%(v/v)Formic acid =
Flow Rate
0.2mL / min.
Detection
MS ESI+
Temperature
40°C
Sample Vol.
10μL
Samples
1. Para red
2. Sudan Ⅰ
3. Sudan Ⅱ
4. Sudan Ⅲ
5. Sudan Ⅳ
Each 5μg/mL
F052
Separation of Aflatoxin G1, G2, B1, B2
Column
Mightysil RP-18 250-4.6mmØ 5μm
Eluent
Methanol/ Water = 1/1
Flow Rate
0.75mL/min.
Detection
Fluorescence 450nm (Excitation 365nm)
Temperature
Room Temperature
Sample Vol.
20μL
Samples
Mixture of samples were pre-treated with trifluoroacetic acid in
order to convert G1 and B1 to their derivatives
1. Aflatoxin G1 derivative
20ng/mL
2. Aflatoxin B1 derivative
20ng/mL
3. Aflatoxin G2
20ng/mL
4. Aflatoxin B2
20ng/mL
FOOD 39
F081
Separation of Fumonisins
Column
Mightysil RP-18 MS 150-2.0mmØ 5μm
Eluent
A:Acetonitrile
B:0.1%Formic acid
Linear gradient:
0min A25% → 5min A50% → 8min A50% → 10min A25% → 25min A25%
Flow Rate
0.2mL/min.
Detection
MS ESI+
Temperature
40°C
Sample Vol.
2μL
Samples
1. Fumonisin B1
2. Fumonisin B3
3. Fumonisin B2 Each 50ppb in Acetonitrile/Water= 50/ 50
F088
F079
Separation of Melamine
Separation of Rutrefactive amines
Column
Mightysil RP-18GP 250-4.6mmØ 5μm
Mightysil RP-8 GP 250-4.6mmØ 5μm
Eluent
Acetonitorile /Water = 60/40
Buffer(pH 3.0)*/Acetonitrile=85/15
[*10 mM Citric acid + 10 mM Sodium octane sulfonate, pH adjust :
NaOH]
Flow Rate
1.0mL/min.
Detection
Fluorescence 525nm (Excitation 325nm)
Temperature
40°C
Flow Rate
1.0mL/min.
Sample Vol.
5μL
Detection
UV240nm
Samples
1. Putrescine
Temperature
40°C
2. Cadaverine
Sample Vol.
10μL
3. Histamine
250μg/mL
Samples
1. Ammelide
4. Tyramine
50μg/mL
2. Ammeline
5. Spermidine
10μg/mL
Column
Eluent
3. Melamine
40 FOOD
Each 0.1mg/mL
I.S : 1,6-Hexanediamine
10μg/mL
10μg/mL
ENVIRONMENT
41
42 ENVIRONMENT
E001
Separation of N-Methylcarbamate derivatives-1
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
A: Water, B: Acetonitrile
Flow Rate
1.0mL/min.
Detection
Fluorescence 445nm
(Excitation 339nm)
Temperature
40°C
Sample Vol.
10μL
Samples
1. Aldicarb sulfoxide
Condition of post labeling
Reaction Coil
5m-0.5mmØ
Teflon
2. Oxamyl
Hydrolyzed by 0.05M NaOH
solution
3. Methomyl
Reagents
Mixture of 10mg/L
o-phthalaldehyde
in 50mM of Na2B4O7
solution
and 50μL/L of
Mercaptoethanol
8. Ethiofencarb
Flow Rate
0.5mL/min.
9. Isoprocarb
Reaction
Temperature
80°C
4. Ethiofencarb sulfoxide
5. Ethiofencarb sulfone
6. Carbofuran-3-keto
7. Carbofuran
10. Methiocarb
E002
Separation of N-Methylcarbamate derivatives-2
Column
Mightysil RP-18 250-4.6mmØ 5μm
Eluent
A: Water B: Tetrahydrofuran C: Acetonitrile
Flow Rate
2.0mL/min.
Detection
Fluorescence 445nm
(Excitation 339nm)
Temperature
40°C
Sample Vol.
10μL
Samples
1. Aldicarb sulfone
Condition of post labeling
Reaction Coil
5m-0.5mmØ
Teflon
Hydrolyzed by 0.05M NaOH
solution
Reagents
Mixture of 10mg/L
o-phthalaldehyde
in 50mM of Na2B4O7
solution
and 50μL/L of
Mercaptoethanol
6. Bendiocarb
Flow Rate
0.3mL/min.
7. Carbaryl
Reaction
Temperature
80°C
2. Ethiofencarb sulfone
3. Aldicarb
4. Propoxur
5. Carbofuran
ENVIRONMENT
43
E003
E004
Separation of Oxine-Copper and Asulam
Separation of Oxine-Copper and Thiram
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 20mM Phoshoric acid = 5/95
Flow Rate
1.0mL/min.
Detection
UV250nm
Temperature
Room Temperature
Sample Vol.
10μL
Samples
1. Oxine-Copper
0.08μg/mL
2. Asulam
0.40μg/mL
0.005AUFS
E005
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 20mM Ammonium acetate buffer (pH3.80*)
[*pH adjust : conc.Acetic acid] = 25/75
Flow Rate
1.0mL/min.
Detection
UV250nm
Temperature
40°C
Sample Vol.
10μL
Samples
1. Oxine-Copper
3μg/mL
2. Thiram
7μg/mL
0.02AUFS
E006
Separation of Simazine, Thiram and Thiobencarb
Separation of Thiram, Iprodione and Bensulide
Column
Mightysil RP-18 150-4.6mmØ 5μm
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 20mM Phosphate buffer(pH3.0)= 55/45
Eluent
Acetonitrile/ Water = 60/40
Flow Rate
1.0mL/min.
Flow Rate
0.8mL/min.
Detection
UV230nm
Detection
UV230nm
Temperature
30°C
Temperature
Room Temperature
Sample Vol.
20μL
Sample Vol.
10μL
Samples
1. Simazine
1μg/mL
Samples
1. Thiram
2. Thiram
1μg/mL
2. Iprodione
10μg/mL
3. Thiobencarb
1μg/mL
3. Bensulide
10μg/mL
44 ENVIRONMENT
0.16AUFS
0.04AUFS
4μg/mL
E007
E008
Separation of Thiram
Separation of Thiophanate-methyl and its metabolite
Column
Mightysil RP-18 150-4.6mmØ 5μm
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
Acetonitrile/ Water = 50/50
Eluent
Methanol/ 10mM Potassium dihydrogenphosphate = 1/1
Flow Rate
1.0mL/min.
Flow Rate
0.8mL/min.
Detection
UV280nm
Detection
UV278nm
Temperature
40°C
Temperature
40°C
Sample Vol.
10μL
Samples
1. Methyl-2-benzimidazole carbamate
Sample
1. Thiram
0.005AUFS
2. Thiophanate-methyl
0.4μg/mL
E009
E011
Separation of Fenitrothion and its metabolite
Separation of Agricultural Chemicals (Germicides)
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 10mM Potassium dihydrogenphosphate (pH3.5) = 55/45
Flow Rate
1.0mL/min.
Column
Mightysil RP-18 150-4.6mmØ 5μm
Detection
UV254nm
Eluent
Methanol/ Water = 60/40
Temperature
Room Temperature
Flow Rate
1.0mL/min.
Sample Vol.
10μL
Detection
UV254nm
Samples
1. Thiophanate-methyl
Temperature
40°C
Sample Vol.
50μL
Samples
1. Nitrocresol
500μg/mL
4. Triflumizole
25μg/mL
2. Fenitrothion
500μg/mL
5. Pencycuron
100μg/mL
0.16AUFS
2. Thiram
3. Diclomezine
25μg/mL
100μg/mL
50μg/mL
ENVIRONMENT
45
E012
E013
Separation of Agricultural Chemicals (Pesticides)
Separation of Agricultural Chemicals (Herbicides)-1
Column
Mightysil RP-18 150-4.6mmØ 5μm
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 10mM Potassium dihydrogenphosphate (pH3.5) = 65/35
Eluent
Acetonitrile/ Water = 75/25
Flow Rate
1.0mL/min.
Flow Rate
1.0mL/min.
Detection
UV225nm
Detection
UV254nm
Temperature
Room Temperature
Temperature
Room Temperature
Sample Vol.
12μL
Sample Vol.
10μL
Samples
1. Dicamba
20μg/mL
Samples
1. Imidacloprid
25μg/mL
2. Bensulfuron-methyl
30μg/mL
2. Fenobucarb
100μg/mL
3. Mefenacet
3. Hexythiazox
300μg/mL
4. Bromobutide
150μg/mL
4. Fluvalinate
60μg/mL
5. Pyributicarb
50μg/mL
0.16AUFS
E014
0.16AUFS
10μg/mL
E015
Separation of Agricultural Chemicals (Herbicides)-2
Separation of 5 Agricultural Chemicals -1
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
A: Acetonitrile/10mM Potassium dihydrogenphosphate (pH2.2) = 5/95
B: Acetonitrile/10mM Potassium dihydrogenphosphate (pH2.2) = 80/20
Linear gradient from 100% of A to 100% of B for 30 min.
Flow Rate
1.0mL/min.
Detection
UV240nm
Temperature
Room Temperature
Sample Vol.
3μL
Samples
1. Oxine-Copper
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
Acetonitrile/ Water = 55/45
Flow Rate
1.0mL/min.
Detection
UV254nm
Temperature
Room Temperature
Sample Vol.
7.5μL
Samples
1. Diuron
25μg/mL
2. Propanil
25μg/mL
2. Asulam
75μg/mL
3. Thiram
0.16AUFS
3. Dymron (Daimuron)
0.04AUFS
40μg/mL
100μg/mL
15μg/mL
4. Chlorpropham
250μg/mL
4. Iprodione
100μg/mL
5. Pyrazolate
125μg/mL
5. Bensulide
200μg/mL
46 ENVIRONMENT
E016
E028
Separation of 5 Agricultural Chemicals -2
Separation of 5 Agricultural Chemicals - 3
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Column
Mightysil RP-18 (L) GP 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 10mM Ammonium acetate buffer(pH3.3*)= 7/ 13 [*pH
adjust : conc.Acetic acid]
Eluent
Acetonitrile/ 10mM Pottasium dihydrogen phosphate Buffer
(pH3.80*) = 1/3
[*pH adjust : H3PO4]
Flow Rate
1.0mL/min.
Detection
UV270nm(0-3min.), UV240nm(3-5.5min.), UV230nm(5.5-14min.)
Flow rate
1.0mL/min.
Temperature
40°C
Temperature
40°C
Sample Vol.
5μL
Sample Vol.
20μL
Samples
1. Asulam
10μg/mL
Samples
1. Asulam
270nm, 1μg/mL
2. Oxine-Copper
10μg/mL
Detection
2. Oxine-Copper
240nm, 1μg/mL
3. Triclopyr
10μg/mL
3. Mecoprop
230nm, 1μg/mL
4. Thiram
10μg/mL
4. Triclopyr
230nm, 1μg/mL
5. Mecoprop
10μg/mL
5. Thiram
270nm, 1μg/mL
E029
E034
Separation of 5 Agricultural Chemicals – 4
Separation of 5 Agricultural Chemicals – 5
Column
Mightysil RP-18 (L) GP 150-4.6mmØ 5μm
Eluent
Acetonitrile / Potassium Phosphate Buffer* = 50/50
[*1.02g of Potassium dihydrogenphosphate was dissolved in 300mL
water, then adjusted pH to 3.5 with H3PO4]
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
A; Water, B; Acetonitrile C; Methanol
Flow rate
1.0mL/min.
Flow Rate
1.0mL/min.
Detection
UV230nm
Detection
UV270nm
Temperature
40°C
Temperature
40°C
Sample Vol.
10μL
Sample Vol.
40μL
Samples
1. Oxine-Copper
5μg/mL
Samples
1. Imidacloprid
0.1μg/mL
Detection
2. Mecoprop
5μg/mL
2. Benomyl
0.1μg/mL
3. Benomyl
1μg/mL
3. Thiophanate-methyl
0.1μg/mL
4. Iprodione
5μg/mL
4. Carbaryl
0.1μg/mL
5. Pencycuron
5μg/mL
5. Linuron
0.1μg/mL
ENVIRONMENT
47
E017
E018
Separation of 6 Agricultural Chemicals
Separation of 9 Agricultural Chemicals
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
Acetonitrile Methanol/ 50mM Potassium dihydrogenphosphate
(pH3.2) = 43/ 9/ 48
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
A: Acetonitrile/ 10mM Potassium dihydrogenphosphate (pH2.2) = 5/95
B: Acetonitrile/ 10mM Potassium dihydrogenphosphate (pH2.2) = 80/20
Linear gradient from 100% of A to 100% of B for 30 min.
Flow Rate
1.0mL/min.
Detection
270nm(0-2.2min.), 240nm(2.2-10min.), 230nm(10-22min.),
220nm(22-27min.), 240nm(27min.-)
Flow Rate
1.0mL/min.
Temperature
40°C
Detection
UV240nm
Sample Vol.
5μL
Temperature
Room Temperature
Samples
1. Asulam
10μg/mL
6. Chlorothalonil
10μg/mL
Sample Vol.
10μL
2. Oxine-Copper
10μg/mL
7. Iprodione
10μg/mL
Samples
1. Oxine-Copper
3. Triclopyr
10μg/mL
8. Bensulide
10μg/mL
9. Pencycuron
10μg/mL
0.16AUFS
2. Asulam
3. Simazine
20μg/mL
4. Thiram
20μg/mL
120μg/mL
5. Iprodione
120μg/mL
4. Thiram
10μg/mL
20μg/mL
6. Bensulide
240μg/mL
5. Mecoprop
10μg/mL
E030
Separation of 12 Agricultural Chemicals
Column
Mightysil RP-18 (L) GP 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 10mM Pottasium dihydrogen phosphate Buffer
(pH3.80*) = 1/3
[*pH adjust : H3PO4]
Flow rate
1.0mL/min.
Detection
0~6min.: 270nm, 6~10.9min.: 240nm, 10.9~24min.: 230nm,
24min.~: 270nm
Temperature
35°C
Sample Vol.
10μL
Samples
1. Asulam
2. Oxine-Copper
Detection
3. Simazine
4. Mecoprop
5. Thiram
6. Siduron
7. Propyzamide
8. Iprodione
9. Chlorothalonil
10. Pencycuron
11. Bensulide
12. Butamifos
Each 10μg/mL
48 ENVIRONMENT
E035
Separation of 9 Agricultural Chemicals – 2
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
A; Water, B; Acetonitrile
Eluent
A; Water, B; Acetonitrile
Flow Rate
1.0mL/min.
Flow Rate
1.0mL/min.
Detection
UV230nm
Detection
UV230nm
Temperature
40°C
Temperature
40°C
Sample Vol.
10μL
Sample Vol.
10μL
Samples
1. hiabendazole
Samples
1. hiabendazole
2. Triadimenol
2. Triadimenol
3. Diflubenzuron
4. Bitertanol
3. Diflubenzuron
5. Pyrazoxyfen
6. Triflumizole metabolite
4. Bitertanol
7. Pyrazolate
8. Teflubenzuron
5. Pyrazoxyfen
9. Chlorfluazuron
Each 25μg/mL
6. Triflumizole metabolite
E036
Separation of 3 Agricultural Chemicals
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
A; 10mM Potassium dihydrogenphosphate(pH3.50*)
[pH adjust :H3PO4]
B; Acetonitrile
Flow Rate
1.0mL/min.
Detection
UV254nm
Temperature
40°C
Sample Vol.
10μL
Sample
1. Destosylpyrazolate
2.5μg/mL
2. Dithianon
2.5μg/mL
3. Triflumizole
2.5μg/mL
0.02AUFS
ENVIRONMENT
49
E045
Separation of Pesticides standard solution 24 [A golf course pesticide]
Column
Mightysil RP-18PA 150-4.6mmØ 5μm
Eluent
Acetonitrile/25mM Phosphate Buffer(pH3.3)=7/13
Flow Rate
1.0mL/min.
Detection
UV270nm (0~2.5min), UV240nm (2.5~5.0min)
UV230nm (5.0~13.0min), UV240nm (13.0~16.0min)
UV255nm (16.0~19.5min), UV245nm (19.5~25.0min)
UV235nm (25.0~35.0min)
Temperature
40°C
Sample Vol.
5μL
Samples
34023-96 Pesticides standard solution 24
1. Asulam
2. Oxine-Copper
3. Triclopyr
4. Thiram
5. Mecoprop
6. Flazasulfuron
7. Siduron
8. Halosulfuron-methyl
9. Azoxystrobin
Each 10ppm
E019
F027
Separation of Intermediates of Agricultural Chemicals
(halogenized Benzoic acids)
Column
Mightysil RP-18 250-4.6mmØ 5μm
Eluent
Acetonitrile/ 0.1% Trifluoroacetic acid = 40/60
Flow Rate
1.0mL/min.
Detection
UV254nm
Temperature
Room Temperature
Sample Vol.
10μL
Separation of Carbofuran
0.256AUFS
Samples
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
1mg/mL each
Eluent
Acetonitrile/ Water = 30/70
Flow rate
1.0mL/min.
Detection
Fluorescence 307nm (Excitation 279nm)
Temperature
40°C
Sample Vol.
50μL
Sample
1. Carbofuran
50 ENVIRONMENT
0.1μg/mL
E032
E033
Separation of Acephate
Separation of Imazalil in Concentrated Lemonade Juice
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Eluent
Acetonitrile/ Water = 5/95
Eluent
Methanol/ 20mM Disodium hydrogen phosphate dihydrate = 3/1
Flow Rate
1.0mL/min.
Flow Rate
1.0mL/min.
Detection
UV214nm
Detection
UV230nm
Temperature
40°C
Temperature
40°C
Sample Vol.
20μL
Sample Vol.
20μL
Sample
1. Acephate
Sample
1. Imazalil
1mg/mL
E040
5mg/mL inLemonade Juice
E041
Separation of Pesticides standard solution 28
Separation of 3 Agricultural Chemicals
Column
Mightysil RP-18PA 150-4.6mmØ 5μm
Eluent
2-Propanol/ Water = 30/70
Flow Rate
1.0mL/min.
Detection
FL Ex : 339nm
Temperature
40°C
Em : 455nm
Column
Mightysil RP-18PA 250-4.6mmØ 5μm
Eluent
Acetonitrile/ 50mM Potassium dihydrogenphosphate(pH3.3*) = 55/
45[*pH adjust : H3PO4]
Flow Rate
1.0mL/min.
Detection
UV270nm Asulam
Post-column reaction sysytem
Reagent1: 50mM NaOH soln. 0.7mL/min
Reaction coil 0.5mmID×5m, 80°C
UV230nm thiophanate-methyl, siduron, iprodion
Reagent2: 6mM o-Phtalaldehyde
Temperature
40°C
30mM 2-Mercaptoethanol
Sample Vol.
20μL
50mM Sodium Tetraborate
Samples
34027-96 Pesticides standard solution 28
1. Asulam
in Methanol/ Water= 1/ 100 0.7mL/min
50ppm
2. Thiophanate-methyl
100ppm
3. Siduron
100ppm
4. Iprodione
50ppm
Cf. Complementary items (for water quality management) ; attached method 9
Sample Vol.
1μL
Samples
1. Methomyl
2. Carbofuran
3. Carbaryl
Each 10ppm
Cf. Complementary items(for water quality management) ; attached method 13
ENVIRONMENT
51
E042
E043
Separation of Carbaryl
Separation of Diquat
Column
Mightysil RP-18PA 150-4.6mmØ 5μm
Eluent
13.5mL/L Phosphoric acid
+ 3.0g/L Sodium 1-pentanesulfonate
+ 10mL/L Diethylamine in water
Column
Mightysil RP-18PA 150-4.6mmØ 5μm
Eluent
Acetonitrile/ Water = 30/70
Flow Rate
1.0mL/min.
Flow Rate
1.0mL/min.
Detection
Fluorescence 307nm (Excitation 279nm)
Detection
UV313nm
Temperature
40°C
Temperature
40°C
Sample Vol.
20μL
Sample Vol.
20μL
Samples
1. Carbaryl
Samples
1. Diquat
1.0ppm
1.0ppm
Cf. Complementary items(for water quality management) ; attached method 10
Cf. Complementary items(for water quality management) ; attached method 11
E044
E039
Separation of LAS
Separation of Iminoctadine triacetate
Column
Mightysil RP-18PA 150-4.6mmØ 5μm
Eluent
Acetonitrile/ 100mM Sodium perchlorate
+0.05% Phosphoric acid in Water =5/17
Column
Mightysil RP-18GP 250-4.6mmØ 5μm
Eluent
0.1M Sodium perchlorate/ Acetonitrile= 65/3
Flow Rate
0.7mL/min.
Flow Rate
1.0mL/min.
Detection
FL Ex 395nm, Em 500nm
Detection
UV225nm
Temperature
40°C
Temperature
40°C
Sample Vol.
10μL
Samples
24289-96 LAS standard stock solution 1000ppm (C10-14 total)
Post-column reaction sysytem
Reagent1: 500mM NaOH soln. 0.2mL/min
Reaction coil 0.5mmID×5m, 80°C
n-Decylbenzenesulfonic acid sodium salt (C10)
Reagent2: 3g/L Ninhydrin soln. 0.2mL/min
Sample Vol.
20μL
Samples
1. Iminoctadine triacetate
n-Undecylbenzenesulfonic acid sodium salt (C11)
n-Dodecylbenzenesulfonic acid sodium salt (C12)
1.0ppm
Cf. Complementary items(for water quality management) ; attached method 15
52 ENVIRONMENT
n-Tridecylbenzenesulfonic acid sodium salt (C13)
n-Tetradecylbenzenesulfonic acid sodium salt (C14)
E010
Separation of 15 Synthetic Antibiotics
Column
Mightysil RP-18GP 250-4.6mmØ 5μm
Eluent
A: Methanol/ 25mM Potassium dihydrogenphosphate = 15/85
B: Methanol/ 25mM Potassium dihydrogenphosphate = 70/30
Flow Rate
0.5mL/min.
Detection
Maximum wavelength detected by photodiode-array
Temperature
40°C
Sample Vol.
20μL
Samples
QCA: 2-Quinoxaline
CDX: Carbadox
PYR: Pyrimethamine
SMR: Sulfamerazine
MRT: Morantel citrate
DFZ: Difurazone
FZD: Furazolidone
SDM: Sulfadimethoxine NAA: Nalidixic acid
SDD: Sulfadimidine
SQX: Sulfaquinoxaline
PMA: Piromidic acid
SMM: Sulfamonomethoxine
OXA: Oxolinic acid
NCZ: Nicarbazin
carboxylic acid
E020
E023
Separation of Benzo[a]pyrene
Separation of Microsystins
Column
Mightysil RP-18 150-4.6mmØ 5μm
Eluent
Methanol/ 50mM Phosphate Buffer(pH3.0) = 58/42
Column
Mightysil RP-18 150-4.6mmØ 5μm
Flow Rate
1.0mL/min.
Eluent
Acetonitrile/ Water = 75/25
Detection
UV240nm
Flow Rate
1.0mL/min.
Temperature
Room Temperture
Detection
UV254nm
Sample Vol.
20μL
Temperature
40°C
Samples
1. Microcystin RR
3.33μg/mL
Sample Vol.
3μL
2. Microcystin YR
3.33μg/mL
Sample
1. Benzo[a]pyrene
3. Microcystin LR
3.33μg/mL
2mg/mL
ENVIRONMENT
53
E046
E024
Separation of Patulin
Separation of Bisphenol A -1
Column
Mightysil RP-18GP 250-4.6mmØ 5μm
Eluent
A: Acetonitrile/ Water = 7/3
B: Acetonitrile
Linear gradient from 100% of A to 100% of B for 33min.
Column
Mightysil RP-18PA 250-4.6mmØ 5μm
Eluent
Acetonitrile/0.082% Sodium perchlorate =3/97
Flow Rate
1.0mL/min.
Flow Rate
1.0mL/min.
Detection
UV276nm
Detection
UV217nm
Temperature
40°C
Temperature
40°C
Sample Vol.
50μL
Sample Vol.
20μL
Samples
1. Patulin
Sample
1. Bisphenol A 0.625ppm (Assayed value)
0.05ppm
E025
E026
Separation of Bisphenol A –2
Separation of Trace Level Bisphenol A
Column
Mightysil RP-18GP 150-4.6mmØ 5μm
Column
Mightysil RP-18GP 250-4.6mmØ 5μm
Eluent
Acetonitrile/ Water = 35/65
Eluent
Acetonitrile/ Water = 45/55
Flow Rate
1.0mL/min.
Flow rate
0.9mL/min
Detection
UV254nm
Detection
UV225nm
Temperature
40°C
Temperature
45°C
Sample Vol.
100μL
Sample Vol.
100μL
Samples
1. Phenol
2.5ppm
Samples
1. Phenol
0.01μg/mL
2. Bisphenol A
2.5ppm
2. Bisphenol A
0.01μg/mL
3. 4-tert-Butylphenol
2.5ppm
3. 4-tert-Butylphenol
0.01μg/mL
54 ENVIRONMENT
0.01AUFS
E031
E021
Separation of 4-Vinylguaiacol
Separation of DNPH-Aldehydes-1
Column
Mightysil RP-18GP 250-4.6mmØ 5μm
Eluent
Phosphate Buffer*/ Methanol = 50/50
[*4.90g/L phosphoric acid/ 6.80g/L Pottasium dihydrogen
phosphate = 1/2]
Column
Mightysil RP-18 250-4.6mmØ 5μm
Eluent
Acetonitrile/ Water = 60/40
Flow Rate
1.0mL/min.
Flow Rate
1.0mL/min.
Detection
UV360nm
Detection
UV260nm
Temperature
40°C
Temperature
40°C
Sample Vol.
20μL
Sample Vol.
5μL
Samples
1. Formaldehyde-2,4-DNPH
1μg/mL (as HCHO)
Sample
1. 4-Vinylguaiacol
2. Acetoaldehyde-2,4-DNPH
1μg/mL (as CH3CHO)
19μg/mL
E022
Separation of DNPH-Aldehydes-2
Column
Mightysil RP-18 250-4.6mmØ 5μm
Eluent
Acetonitrile/ Water = 75/25
Flow Rate
1.0mL/min.
Detection
UV385nm
Temperature
40°C
Sample Vol.
1μL
Samples
1. Formaldehyde-2,4-DNPH
100μg/mL
2. Acetaldehyde-2,4-DNPH
100μg/mL
3. Acrolein-2,4-DNPH
100μg/mL
4. Methylethylketone-2,4-DNPH
200μg/mL
5. Methylisobutylketone-2,4-DNPH
200μg/mL
6. Isophorone-2,4-DNPH
200μg/mL
ENVIRONMENT
55
E037
Separation of DNPH-Aldehydes-3
Column
Mightysil DNPH 250-4.6mmØ 5μm
Eluent
A: Water
B: Methanol
Flow Rate
1.0mL/min.
Detection
UV360nm
Temperature
40°C
Sample Vol.
10μL
Samples
1. Formaldehyde-DNPH
6. Crotonaldehyde-DNPH
2. Acetaldehyde-DNPH
7. Butylaldehyde-DNPH
3. Acrolein-DNPH
8. Benzaldehyde-DNPH
4. Acetone-DNPH
5. Propionaldehyde-DNPH
9. iso-Valeraldehyde-DNPH
10. Valeraldehyde-DNPH
Each 1ppm
E038
Separation of DNPH-Aldehydes-4
Column
Mightysil DNPH 250-4.6mmØ 5μm
Eluent
A: Water
B: Acetonitrile
Flow Rate
1.0mL/min.
Detection
UV360nm
Temperature
40°C
Sample Vol.
10μL
Samples
1. Formaldehyde-DNPH
6. Crotonaldehyde-DNPH
Each 1ppm
2. Acetaldehyde-DNPH
7. Butylaldehyde-DNPH
3. Acrolein-DNPH
8. Benzaldehyde-DNPH
4. Acetone-DNPH
5. Propionaldehyde-DNPH
9. iso-Valeraldehyde-DNPH
10. Valeraldehyde-DNPH
01106-96 Aldehydes-2,4-DNPH standard stock solution Ⅱ
56 ENVIRONMENT
INDEX
CONPOUND NAME
SHEET NO. (PAGE)
A
CONPOUND NAME
SHEET NO. (PAGE)
Bisphenol A
Acephate
E032 (51)
Acesulfame K
F069 (25), F080 (25)
Acetaldehyde-2,4-DNPH
Acetic acid
E021 (55), E022 (55), E037 (56), E038 (56)
F098 (36)
Acetone-2,4-DNPH
E037 (56), E038 (56)
E024 (54), E025 (54), E026 (54)
Bitertanol
E035 (49)
Blue-1, Brilliant blue FCF
F038 (22), F040 (23), F072 (24)
Blue-1 Aluminium Lake, Brilliant blue FCF Aluminium Lake
F041 (23)
Blue-2, Indigocarmine
F038 (22), F040 (23), F072 (24)
Brilliant blue FCF, Blue-1
F038 (22), F040 (23), F072 (24)
Acid red, Red-106
F039 (22), F040 (23), F072 (24)
Brilliant blue FCF Aluminium Lake, Blue-1 Aluminium Lake
F041 (23)
Acrolein-2,4-DNPH
E022 (55), E037 (56), E038 (56)
Bromobutide
E013 (46)
Acrylic acid
F036 (36), F094 (36), F098 (36)
Butamifos
E030 (48)
Acylanthocyanin
F060 (24)
Adipic acid
F032 (35), F092 (35)
Butylaldehyde-2,4-DNPH
E037 (56), E038 (56)
2-tert-Butyl hydroquinone, BHQ
F042 (20), F073 (20)
Aflatoxin B1
F052 (39)
Butyl hydroquinone, 2-tert-Butyl hydroquinone
F042 (20), F073 (20)
Aflatoxin B2
F052 (39)
tert-Butylhydroxyanisole, BHA
F042 (20), F073 (20)
Aflatoxin G1
F052 (39)
Butyl hydroxyanisole, tert-Butylhydroxyanisole
F042 (20), F073 (20)
Aflatoxin G2
F052 (39)
Butyl hydroxytoluene, BHT
F042 (20), F073 (20)
Albendazole
F023 (17)
n-Butyl paraben, n-Butyl p-hydroxybenzoate
F043 (18), F074 (19)
Aldicarb
E002 (43)
2,6-di-tert-Butyl-p-cresol, Butyl hydroxytoluene
F042 (20), F073 (20)
Aldicarb sulfone
E002 (43)
4-tert-Butylphenol
E025 (54), E026 (54)
Aldicarb sulfoxide
E001 (43)
n-Butyl p-hydroxybenzoate, n-Butyl paraben F043 (18), F074 (19), F077 (19)
Allura Red AC, Red-40
F072 (24)
Allyl isothiocyanate
F063 (29)
Amaranth, Red-2
F039 (22), F040 (23), F072 (24)
C
Cadaverine
Ammelide
F088 (40)
Caffeine
Ammeline
F088 (40)
Capsaicin
F011 (11), F012 (11)
Carbadox
Amprolium
F079 (40)
F050 (24), F053 (27), F054 (27), F055 (28), F056 (28)
F065 (29)
E010 (53), F013 (12), F026 (13)
Anthocyanin
F060 (24)
Carbaryl
E002 (43), E034 (47), E041 (51), E042 (52)
Apigenin
F099 (30)
Carbofuran
E001 (43), E002 (43), E027 (50), E041 (51)
F048 (21)
Carbofuran-3-keto
Ascorbic acid
L-Ascorbic acid, Vitamin C
F058 (08), F070 (07)
β-Carotene
Aspartame
F050 (24), F080 (25)
Catechin
Asulam
E003 (44), E015 (46), E016 (47), E017 (48), E018 (48),
E028 (47), E030 (48), E040 (51), E045 (50)
E001 (43)
F004 (09), F059 (09), F071 (08)
F053 (27), F054 (27), F055 (28), F056 (28)
Catechin gallate
F054 (27), F055 (28), F056 (28)
Chlorfluazuron
E035 (49)
Avoparcin mixture
F031 (17)
Chlorophyll a
F056 (28)
Azoxystrobin
E045 (50)
Chlorothalonil
E018 (48), E030 (48)
Chlorpropham
E014 (46)
B
Chlortetracycline
F006 (13)
Bendiocarb
E002 (43)
Cholecalciferol, Vitamin D3
Benofloxacin
F007 (09)
Chrysin
Benomyl
E029 (47), E034 (47)
Bensulfuron methyl
Bensulide
E013 (46)
E006 (44), E015 (46), E017 (48), E018 (48), E030 (48)
Benzaldehyde-2,4-DNPH
E037 (56), E038 (56)
Citric acid
F103 (31)
F035 (35), F036 (36), F093 (35), F094 (36), F098 (36)
Clopidol
Closantel
Coenzyme Q10, Ubiquinone 10
Benzo[a]pyrene
E020 (53)
Coenzyme Q9, Ubiquinone 9
5,6-Benzoflavone
F100 (30)
Crotonaldehyde-2,4-DNPH
7,8-Benzoflavone
F101 (31)
β-Cryptoxanthin
Benzoic acid
F044 (18), F047(18), F050 (24), F061 (20), F075 (18) ,F077(19)
Benzoyl peroxide
F091 (26)
BHA, tert-Butylhydroxyanisole
F042 (20), F073 (20)
BHQ, 2-tert-Butyl hydroquinone
F042 (20), F073 (20)
BHT, Butyl hydroxytoluene
F042 (20), F073 (20)
Biochanin A
Biphenyl
F102 (31)
F045 (21), F046 (21)
F003 (07), F005 (06)
Curcumin
F013 (12)
F014 (14), F020 (16)
F089 (26)
F089 (26)
E037 (56), E038 (56)
F071 (08)
F082 (29), F083 (30)
Cyanocobalamin, Vitamin B12
F097 (08)
Cyclamic acid
F076 (25)
D
Daimuron, Dymron
E014 (46)
Danofloxacin
F007 (09)
INDEX
57
INDEX
CONPOUND NAME
SHEET NO. (PAGE)
CONPOUND NAME
Danofloxacin mesylate
F008 (10)
Formaldehyde-2,4-DNPH
n-Decylbenzenesulfonic acid sodium salt
E039 (52)
Formic acid
SHEET NO. (PAGE)
E021 (55), E022 (55), E037 (56), E038 (56)
F062 (36)
Dehydroacetic acid
F044 (18), F047 (18), F075 (18)
Destosylpyrazolate
E036 (49)
Fumonisin B1
F081 (40)
DG, Dodecyl gallate
F073 (20)
Fumonisin B2
F081 (40)
Dicamba
E013 (46)
Fumonisin B3
F081 (40)
Diclomezine
E011 (45)
Furazolidone
E010 (53), F010 (11), F013 (12)
Diflubenzuron
Fumaric acid
F036 (36), F094 (36), F098 (36)
E035 (49)
Difurazone
E010 (53), F013 (12)
G
Dihydrocapsaicin
F065 (29)
Gallic acid
2,5-Dimethylpyrazine
F051 (26)
Gallocatechin gallate
F054(27), F055 (28), F056 (28)
Diosmin
F104 (31)
Gentisic acid
F034 (37), F095 (37), F096 (37)
Diquat
E043 (52)
Gleen-3, Fast green FCF
F038 (22), F040 (23), F072 (24)
Dithianon
E036 (49)
Glucose
Diuron
E014 (46)
Glutaric acid
F032 (35), F092 (35)
n-Dodecylbenzenesulfonic acid sodium salt
E039 (52)
Glycolic acid
F035 (35), F093 (35), F098 (36)
Dodecyl gallate, DG
F073 (20)
Doxycycline
F006 (13)
H
Dymron, Daimuron
E014 (46)
Halogenated benzoic acid
E019 (50)
Halosulfuron-methyl
E045 (50)
Hesperetin
F106 (32)
Hesperidin
F107 (32)
E
Enrofloxacin
F007 (09), F008 (10)
Eosin
F072 (24)
Eosin Y
F039 (22), F040 (23)
F034 (37), F054 (27), F095 (37), F096 (37)
F066 (34), F068 (34)
1,6-Hexanediamine
F079 (40)
Hexythiazox
E012 (46)
Epicatechin
F053 (27), F054 (27), F055 (28), F056 (28)
Histamine
F079 (40)
Epicatechin gallate
F053 (27), F054 (27), F055 (28), F056 (28)
HMBP, Hydroxymethylbutylphenol
F073 (20)
Epigallocatechin
F053 (27), F054 (27), F055 (28), F056 (28)
Hydroxymethylbutylphenol, HMBP
F073 (20)
Epigallocatechin gallate
F053 (27), F054 (27), F055 (28), F056 (28)
5-Hydroxythiabendazole
Ergocalciferol, Vitamin D2
Erythorbic acid
Erythrosine, Red-3
F048 (21)
F039 (22), F040 (23), F072 (24)
I
Imazalil
E033 (51), F045 (21)
E012 (46), E034 (47)
Erythrosine Aluminium Lake, Red-3 Aluminium Lake
F041 (23)
Imidacloprid
Ethiofencarb
E001 (43)
Iminoctadine triacetate
Ethiofencarb sulfone
Ethiofencarb sulfoxide
Ethopabate
Ethyl p-hydroxybenzoate, Ethyl paraben
Ethyl paraben, Ethyl p-hydroxybenzoate
E001 (43), E002 (43)
Iprodione
F011 (11)
F043 (18), F069 (25), F074 (19),
F077(19)
F043 (18), F069 (25), F074 (19),
F077 (19)
E044 (52)
Indigocarmine, Blue-2
E001 (43)
F
Fast green FCF, Gleen-3
F015 (14), F030 (16)
F003 (07), F005 (06)
F038 (22), F040 (23), F072 (24)
E006 (44), E015 (46), E017 (48), E018 (48),
E029 (47), E030 (48), E040 (51)
Isobutyl p-hydroxybenzoate, Isobutyl paraben F043 (18), F074 (19), F077 (19)
Isobutyl paraben, Isobutyl p-hydroxybenzoate
F043 (18), F074 (19), F077 (19)
Isometamidium
F025 (17)
Isophorone-2,4-DNPH
E022 (55)
Isoprocarb
E001 (43)
Isopropyl p-hydroxybenzoate, Isopropyl paraben F043 (18), F074 (19), F077(19)
F038 (22), F040 (23), F072 (24)
Isopropyl paraben, Isopropyl p-hydroxybenzoate
F043 (18), F074 (19), F077 (19)
Fenethylcyanate
F063 (29)
Isoquercitrin
Fenitrothion
E009 (45)
iso-Valeraldehyde-2,4-DNPH
E037 (56), E038 (56)
Fenobucarb
E012 (46)
Ivermectin
F014 (14), F018 (15)
Flavonol
F105 (32)
Flazasulfuron
E045 (50)
Flubendazole
F014 (14), F022 (16)
Flumequine
F007 (09)
Fluvalinate
E012 (46)
Folic acid, Vitamin B9
58 INDEX
F001 (05), F067 (06)
F108 (32)
K
Kaempferol
F109 (33)
L
Lactic acid
F035 (35), F093 (35), F098 (36)
INDEX
CONPOUND NAME
SHEET NO. (PAGE)
LAS
Leucomalachite green
Linuron
CONPOUND NAME
SHEET NO. (PAGE)
E039 (52)
Olaquindox
F013 (12)
F084 (38), F085 (38)
Orbifloxacin
F007 (09)
Ormethoprim
F013 (12)
E034 (47)
Lutein
F071(08)
Oxalic acid
Luteolin
F110 (33)
Oxamyl
Lycopene
F071 (08)
Oxine-copper
E003 (44), E004 (44), E015 (46), E016 (47), E017 (48),
E018 (48), E028 (47), E029 (47), E030 (48), E045 (50)
M
F033 (37), F035 (35), F036 (36), F093 (35), F094 (36)
E001 (43)
Oxolinic acid
Malachite green
F084 (38), F085 (38)
Maleic acid
E010 (53), F007 (09), F012 (11), F013 (12)
Oxytetracycline
F006 (13), F014 (14), F019 (14)
F036 (36), F094 (36), F098 (36)
Malic acid
F035 (35), F036 (36), F093 (35), F094 (36), F098 (36)
P
Malonic acid
F032 (35), F036 (36), F092 (35), F094 (36), F098 (36)
Pantothenic acid calcium salt
F002 (05), F067 (06)
Maltoheptaose
F066 (34)
Para red
F086 (38), F087 (39)
Maltohexaose
F066 (34)
Patulin
Maltopentaose
F066 (34)
Pencycuron
Maltose
F066 (34)
2,2,5,7,8-Pentamethylhydroxychroman
Maltotetraose
F066 (34)
PG, Propyl gallate
Maltotriose
F066 (34)
Phenol
E046 (54)
E011 (45), E018 (48), E029 (47), E030 (48)
F057 (07)
F073 (20)
E025 (54), E026 (54)
Mecoprop
E016 (47), E018 (48), E028 (47), E029 (47), E030 (48), E045 (50)
Mefenacet
E013 (46)
Phloxine, Red-104
F039 (22), F040 (23), F072 (24)
Melamine
F088 (40)
Phthalic acid
F034 (37), F095 (37), F096 (37)
Menadione, Vitamin K3
F005 (06)
Phylloquinone, Vitamin K1
E001 (43)
Pimelic acid
Methiocarb
Methomyl
E001 (43), E041 (51)
o-Phenylphenol
Piromidic acid
F045 (21), F046 (21)
F005 (06)
F032 (35), F092 (35)
E010 (53), F007 (09), F013 (12)
p-Methylacetophenon
F064 (29)
Propanil
Methyl-2-benzimidazole carbamate
E008 (45)
Propionaldehyde-2,4-DNPH
Methylethylketone-2,4-DNPH
E022 (55)
Propoxur
E002 (43)
Propyl gallate, PG
F073 (20)
Methyl p-hydroxybenzoate, Methyl paraben
Methylisobutylketone-2,4-DNPH
Methyl paraben, Methyl p-hydroxybenzoate
2-Methylpyrazine
Microcystin
Morantel citrate
F043 (18), F074 (19)
E022 (55)
n-Propyl p-hydroxybenzoate, n-Propyl paraben F043 (18), F074 (19), F077 (19)
F043 (18), F074 (19)
n-Propyl paraben, n-Propyl p-hydroxybenzoate F043 (18), F074 (19), F077 (19)
F051 (26)
5-Propylsulfonyl-1H-benzimidazole-2-amine
E023 (53)
Propyzamide
E010 (53), F013 (12)
N
Nalidixic acid
E014 (46)
E037 (56), E038 (56)
E010 (53), F007 (09), F013 (12)
Protocatechuic acid
F015 (14), F024 (17)
E030 (48)
F034 (37), F095 (37), F096 (37)
Putrescine
F079 (40)
Pyrazin
F051 (26)
Pyrazolate
E014 (46), E035 (49)
Naringenin
F111 (33)
Pyrazoxyfen
E035 (49)
Naringin
F112 (33)
Pyributicarb
E013 (46)
F073 (20)
Pyridoxine hydrochloride, Vitamin B6
F001 (05), F002 (05), F067 (06)
Pyrimethamine
E010 (53), F011 (11), F013 (12)
NDGA, Nordihydroguaiaretic acid
New coccine, Red-102
F039 (22), F040 (23), F072 (24)
Nicarbazin
E010 (53), F011 (11), F013 (12)
Nicotinamide
F001 (05), F002 (05), F067 (06)
Nicotinic acid
F001 (05), F067 (06)
Q
Quassin
F064 (29)
Nitric acid
F033 (37)
Quercetin
F078 (30)
Nitrocresol
E009 (45)
2-Quinoxalinecarboxylic acid
Nitrofurazone
F010 (11)
Nordihydroguaiaretic acid, NDGA
F073 (20)
R
Raffinose
O
E010 (53), F015 (14), F027 (13)
F068 (34)
Red-102, New coccine
F039 (22), F040 (23), F072 (24)
Octyl gallate, OG
F073 (20)
Red-104, Phloxine
F039 (22), F040 (23), F072 (24)
Ofloxacin
F007 (09)
Red-105, Rose bengal
F039 (22), F040 (23), F072 (24)
OG, Octyl gallate
F073 (20)
Red-106, Acid red
F039 (22), F040 (23), F072 (24)
INDEX
59
INDEX
CONPOUND NAME
SHEET NO. (PAGE)
CONPOUND NAME
SHEET NO. (PAGE)
Red-2, Amaranth
F039 (22), F040 (23), F072 (24)
Thiram
Red-3, Erythrosine
F039 (22), F040 (23), F072 (24)
E015 (46), E016 (47), E017 (48), E 018 (48), E028 (47), E030 (48), E045 (50)
E004 (44)E005 (44), E006 (44), E007 (45), E011 (45),
Red-3 Aluminium Lake, Erythrosine Aluminium Lake
F041 (23)
Tocopherol, Vitamin E
Red-40, Allura Red AC
F072 (24)
α-Tocopherol
F057 (07)
Retinol acetate, Vitamin A acetate
F005 (06)
β-Tocopherol
F057 (07)
F113 (34)
γ-Tocopherol
F057 (07)
F001 (05), F002 (05), F067 (06)
δ-Tocopherol
F057 (07)
Rhoifolin
Riboflavin, Vitamin B2
Riboflavin phosphate sodium salt
F001 (05), F067 (06)
Rose bengal, Red-105
F039 (22), F040 (23), F072 (24)
Rutin
F078 (30)
F005 (06), F058 (08)
Tocopherol acetate, Vitamin E acetate
F014 (14), F016 (15)
β-Trenbolone
F014 (14), F017 (15)
Triadimenol
S
Triclopyr
Saccharin
Salicylic acid
F049 (25), F061 (20), F077 (19)
F034 (37), F075 (18), F095 (37), F096 (37)
F005 (06)
α-Trenbolone
E035 (49)
E016 (47), E018 (48), E028 (47), E045 (50)
n-Tridecylbenzenesulfonic acid sodium salt
Triflumizole
E039 (52)
E011 (45), E036 (49)
Siduron
E030 (48), E040 (51), E045 (50)
Triflumizole metabolite
E035 (49)
Simazine
E005 (44), E017 (48), E030 (48)
Trihydroxybutyrophenone, THBP
F073 (20)
4,2',4'-Trihydroxychalcone
F114 (34)
Trimethoprim
F013 (12)
2,3,5-Trimethylpyrazine
F051 (26)
Tyramine
F079 (40)
Sorbic acid
F044 (18), F047 (18), F049 (25), F061 (20),
F075 (18), F077 (19)
Spermidine
Succinic acid
F032 (35), F092 (35), F098 (36)
Sucrose
F068 (34)
Sudan Ⅰ
F086(38), F087(39)
Sudan Ⅱ
F086 (38), F087 (39)
Ubiquinone 10, Coenzyme Q10
F089 (26)
Sudan Ⅲ
F086(38), F087 (39)
Ubiquinone 9, Coenzyme Q9
F089 (26)
Sudan Ⅳ
F086 (38), F087 (39)
n-Undecylbenzenesulfonic acid sodium salt
E039 (52)
Sulfachlorpyridazine
U
F009(10)
Sulfadimethoxine
E010 (53), F009 (10), F 013 (12)
Sulfadimidine
E010 (53), F013 (12), F015 (14), F028 (13)
Sulfamerazine
E010 (53), F009 (10), F013 (12)
V
Valeraldehyde-2,4-DNPH
4-Vinylguaiacol
Sulfamethoxazole
F009 (10)
Vitamin A acetate, Retinol acetate
Sulfamethoxypyridazine
F009 (10)
Vitamin B1, Thiamine hydrochloride
Sulfamonomethoxine
E010 (53), F009 (10), F013 (12)
Sulfaquinoxaline
E010 (53), F013 (12)
Vitamin B12, Cyanocobalamin
E037 (56), E038 (56)
E031 (55)
F005 (06)
F001 (05), F067 (06)
F097 (08)
Vitamin B2, Riboflavin
F001 (05), F002 (05), F067 (06)
F001 (05), F002 (05), F067 (06)
Sulfathiazole
F009 (10)
Vitamin B6, Pyridoxine hydrochloride
Sulfisomidine
F009 (10)
Vitamin B9, Folic acid
F001 (05), F067 (06)
Vitamin C, L-Ascorbic acid
F058 (08), F070 (07)
Vitamin D2, Ergocalciferol
F003 (07), F005 (06)
Vitamin D3, Cholecalciferol
F003 (07), F005 (06)
F005 (06), F058 (08)
Sunset yellow FCF, Yellow-5
F037 (21), F040 (23), F072 (24)
T
Tartaric acid
F036 (36), F062 (36), F094 (36)
Vitamin E, Tocopherol
Tartrazine, Yellow-4
F037 (21), F040 (23), F072 (24)
Vitamin E acetate, Tocopherol acetate
F005 (06)
Tartrazine Aluminium Lake, Yellow-4 Aluminium Lake
F041 (23)
Vitamin K1, Phylloquinone
F005 (06)
Teflubenzuron
E035 (49)
Vitamin K3, Menadione
F005 (06)
Tetracycline
F006 (13)
n-Tetradecylbenzenesulfonic acid sodium salt
E039 (52)
Y
2,3,5,6-Tetramethylpyrazine
F051 (26)
Yellow-4, Tartrazine
THBP, Trihydroxybutyrophenone
F073 (20)
Yellow-5, Sunset yellow FCF
Theogallin
Thiabendazole
F054 (27)
E035 (49), F029 (16), F045 (21), F046 (21)
Thiamine hydrochloride, Vitamin B1
F001 (05), F067 (06)
Thiobencarb
E005 (44)
DL-Thioctic acid
F090 (26)
Thiophanate-methyl
60 INDEX
F037 (21), F072 (24)
F037 (21), F040 (23), F072 (24)
E008 (45), E011 (45), E034 (47), E040 (51)
Z
Zeranol
F014 (14), F021 (15)
02 CONTENTS