___________________________________________ DNA Learning Center’s ___________________________________________ Welcome to the Spring 2015 Saturday DNA! Programs! Come to the science scene on Saturdays!! Infection Detection Amid Plasmids DNA Runaway! ____________________ Join us for exciting, new Saturday DNA! sessions! Add some DNA to your day . . . One session is offered for each Saturday in this newsletter. The April and June sessions are for ages 10-13 with an accompanying chaperone. The May session is for students ages 14 to adult with accompanying chaperone for participants under 15. All sessions are two hours long: 1:00 - 3:00 p.m. Chaperones fully participate in all program activities. Sessions are $15.00 per person ($30.00 for a participating student and accompanying chaperone.) What you need to do: • You must read and follow the instructions on the Reservation Guidelines. Applications not adhering to these guidelines will be returned. • Download reservation form(s) from the Saturday DNA! link on our website at www.dnalc.org. • • Reserve your space by completing and returning a reservation form with your payment. You must include a separate check for each session you plan to attend. Information hotline: (516) 367-5168. Mailing Address: Location of Event: DNA Learning Center Cold Spring Harbor Laboratory One Bungtown Road Cold Spring Harbor, NY 11724 Dolan DNA Learning Center 334 Main Street Cold Spring Harbor, NY 11724 Directions to the DNA Learning Center and Reservation Guidelines are on the website at: www.dnalc.org DNA Learning Center’s ___________________________________________________________ Saturday, April 25, 2015 Bacteria and Antibiotics 1:00 – 3:00 p.m. Ages 10-13, chaperone required How can you kill something that can’t be seen with the naked eye? Bacteria are microscopic organisms that live on nearly every surface you encounter. Come and explore the many varieties of bacteria and the complicated role that antibiotics play in the treatment of bacterial infections. In this activity, participants will: • observe the effect of two separate antibiotics on two different strains of bacteria; • understand the function of different antibiotics; • learn the history of antibiotics and disease treatment. ___________________________________________________________ Saturday, May 16, 2015 The Genetic Engineering Toolbox 1:00 – 3:00 p.m. Ages 14 – Adult; under 15 years, chaperone required The term “plasmid” dates back to 1952 when it was first used to describe a small piece of bacterial DNA that is separate from the chromosomal DNA. In nature, plasmids carry genes that can help bacteria survive in adverse conditions. Plasmids can also be created in the lab. These artificial plasmids are used as vectors in genetic engineering, allowing organisms to express traits they never could naturally. Join us for a closer look at plasmids and how they are used in genetic engineering. In this lab session, you will: • use restriction enzymes to digest artificial plasmids; • analyze plasmid maps to predict the results of your restriction digests; • visualize your results using gel electrophoresis. ___________________________________________________________ Saturday, June 6, 2015 Going the Distance 1:00 – 3:00 p.m. Ages 10-13, chaperone required Most of the time when scientists work with DNA, they are unable to see it. In the 1970s, a powerful tool called DNA gel electrophoresis was developed that finally allowed scientists to visualize DNA molecules. This process uses electricity to separate DNA fragments by size as they migrate through a gel matrix. Come join us and try out this technique for yourself! Students will: • learn the basic principles of gel electrophoresis; • make an agarose gel and load samples of DNA; • analyze results using UV light.