rd
ISDSB 2010 – 3 International Symposium on
Diffraction Structural Biology
th
May 25-28 , 2010, Auditorium Pierre
Lehmann, LAL, Bldg 200
Paris-Sud/XI University, Orsay, France
Programme
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Tuesday, May 25
12:00 Registration Auditorium Pierre Lehmann, LAL, Bldg. 200 Paris Sud/XI University,
Orsay, France
14:00 – 14:30
Welcome
M. Van der Rest, SOLEIL (SOLEIL DG), representatives of Region Ile de France
and Paris-Sud University & J. Takahara (Minister Plenipotentiary, Embassy of
Japan in France)
14:30 – 15:30
Opening lecture
Chair: Roger Fourme
What we have learnt from the structures of ribosome.
Venki Ramakrishnan, MRC LMB Cambridge, UK
15:30 – 16:00
Coffee break
16:00 – 18:00
S1. Electron microscopy, X-ray imaging, tomography
Chair: Wolfgang Baumeister
Electron cryo-tomography of eukaryotic flagella and cilia.
Takashi Ishikawa, ETH Zurich, Switzerland
Electron microscopy of membrane proteins - from atomic structure to in situ
arrangement. Werner Kühlbrandt, MPI, Frankfurt, Germany
Imaging cells using correlated fluorescence and x-ray tomography.
Carolyn Larabell , UCSF, San Francisco, CA, USA
Electron and X-ray tomography of the malaria parasite, P. falciparum.
Leann Tilley, La Trobe U., Melbourne, Australia
18:00 – 18:30
Transfer to Synchrotron SOLEIL (Saint Aubin) by bus
18:30 – 20:30
Welcome party at Synchrotron SOLEIL
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Wednesday, May 26
09:00 – 09:45
Plenary talk
Chair: Ian Wilson
Synchrotrons, high throughput crystallography and discovery of new medecines:
drugging the undruggable. Tom Blundell, Cambridge U., UK
09:45 – 10:15
Coffee break
10:15 – 12:45 S2. Drug and vaccine design
Chair: Tom Blundell
Structure-based vaccine design. Phillip
Dormitzer, Novartis, Cambridge, MA, USA
Drug discovery facilitated by biophysical methods.
Michael Hennig, Hoffmann-La Roche, Basel, Switzerland
Chemogenomic profiling in structure-based drug design: what are optimal
ligandbinding parameters for a given target? Gerhard Klebe, Marburg U.,
Germany
Structural genomics of the human G-protein coupled receptor family.
Raymond Stevens, Scripps Research Institute, La Jolla, CA, USA
Drug design of hematopoietic prostaglandin D synthase inhibitors based on
high resolution X-ray crystallography. Yoshihiro Urade, Osaka Bioscience
Institute, Osaka, Japan
13:00 – 14:30 Lunch at University Restaurant – Bldg 230
14:30 – 16:00 S3. Protonation States
Chair: John Helliwell
Protons in proteins. Matthew Blakeley,
ILL, Grenoble, France
Diisopropyl fluorophosphatase: from protonation states to protein engineering.
Julian Chen, Institute of Biophysical Chemistry, Goethe University, Frankfurt,
Germany
Histidine protonation states in a human hemoglobin. Yukio Morimoto ,
Research Reactor Inst., U. Kyoto, Kumatori, Osaka, Japan
16:00 – 16:30 Coffee break
16:30 – 18:00 S4. Large Bio Molecules
Chair: Jack Johnson
Seeing the structural organization of the actin cytoskeleton at the lamella by in situ
cryo-electron tomography. Dorit Hanein, Burnham Inst. for Medical Research, La
Jolla, CA, USA
3D architecture and allosteric changes for cell entry of the envelope glycoprotein
shell of Chikungunya virus. Felix Rey, Institut Pasteur, Paris, France
Ribosome dynamics and tRNA movement as visualized by time-resolved electron
cryomicroscopy. Holger Stark, MPI, Göttingen, Germany
18:00 – 18:45 Plenary talk
Chair: Wayne Hendrickson
Exploiting the anisotropy of anomalous scattering boosts the phasing power of SAD
and MAD experiments. Gérard Bricogne, Global Phasing Ltd, Cambridge, UK
19:00 Transfer for a boat trip with dinner on Seine
20:00 – 22:30 Conference Dinner on Seine
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Thursday May 27
08:45 – 09:30
Bricogne
Plenary talk Chair: Gérard
Small angle X-ray scattering of biological macromecules.
Dmitri Svergun, EMBL, Hamburg, Germany
09:30 – 09:50
Coffee break
09:50 – 11:20 S5. Membrane Proteins
Chair: Gebhard Schertler
Crystal structures of the autotransporter EspP before and after passenger cleavage
reveals a novel outer membrane cleavage. Susan Buchanan, NIDDK, Bethesda,
MD, USA
Structure of photosystem complex around oxygen-evolving Mn4 OxCa-cluster.
Nobuo Kamiya, Osaka City U., Osaka, Japan
Molecular basis of the alternating access model of membrane transport by the
sodium-hydantoin transporter, Mhp1. Alex Cameron, MPL Diamond, Chilton,
UK
11:20 – 11:40
Coffee break
11:40 – 13:15 S6. Protein Structure/Function
Chair: Nori Yasuoka
Allosteric control of nuclear receptors dimerization. Dino Moras,
IGBMC, CNRS-INSERM-ULP, Strasbourg, France
Glutamate dehydrogenase: structure, allostery, evolution, and role in insulin
homeostasis. Thomas J. Smith, Danforth Plant Science Center, Saint Louis,
MI, USA
Structures, regulation and evolution of glutamate synthases.
Maria Vanoni, U. of Milano, Milano, Italy
13:15 – 14:30
Lunch at University Restaurant – Bldg 230
14:30 – 15:00
Transfer to Synchrotron SOLEIL by bus
15:00 – 19:00
Poster session I and commercial exhibitors
Visit of the facility
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Friday May 28
08:45 – 09:30
Plenary talk
Chair: Claudio Luchinat
Structure-inspired functional discovery for a family of novel ion channels.
Wayne Hendrickson, Columbia U., New York, USA
09:30 – 09:50
Coffee break
09:50 – 12:00
S7. Coupling NMR/XRD, X-ray technologies
Chair: Nori Sakabe
Small angle X-ray data and paramagnetism-based NMR restraints are highly
complementary in defining the conformational space sampled by flexible twodomain proteins. Claudio Luchinat, CERM, U. of Florence, Italy
Microbeam macromolecular crystallography and reduced radiation damage.
Robert Fischetti, APS, Argonne, USA
High-pressure macromolecular crystallography status and applications.
Eric Girard, IBS, Grenoble, France
The future of micro- and nano-diffraction of membrane protein samples.
Gebhard Schertler, Paul Scherrer Institute, Villigen, Switzerland
Provision for hot topic
12:00 – 13:15
Poster session II (LAL, Salle Bleue – Bldg 200)
13:15 – 14:30
Lunch at University Restaurant – Bldg 230
14:30 – 15:15
Blakeley
Plenary talk Chair: Matthew
Neutrons in diffraction structural biology making the best use of neutrons.
Ichiro Tanaka, Ibaraki U., Japan
15:15 – 15:45
Coffee break
15:45 – 17:15
S8. Structural Genomics
Chair: Dino Moras
Structural genomics and the expanding protein universe. Ian
Wilson , JCSG, Scripps Research Inst., La Jolla, CA, USA
Non-protein components of protein structures and other biomedical aspects of
structural genomics. Wladek Minor, MCSG, CSGID, U. of Virginia,
Charlottesville, VA, USA
Family wide structural and functional analysis of the human proteome.
Stefan Knapp, SGC, U. of Oxford, Oxford, UK
17:15 - 18:00
Plenary talk
Chair: Leann Tilley
Electron cryomicroscopy: from molecules to systems.
Wolfgang Baumeister, MPI Martinsried, Germany
18:00 – 18:15
Closing Ceremony
Poster Presentations
SESSION I
PO-SI-01
Structural Studies and Sequencing of the Giant Haemoglobin from
Glossoscolex Paulistus
Bachega, J.F.R.,
PO-SI-02
Ultrastructural Organization of Budding Yeast Septin Filaments Both in
vitro and in situ, Analyzed by Electron Microscopy
Bertin A.
PO-SI-03
High Resolution Crystal Structure of the E.coli Maltoporin
Blaise M.
PO-SI-04
Capsid
Bacteriophage T5, a Model System for Investigating the Assembly Pathway of a T=13
Boulanger P.
PO-SI-05
polymerases
Structural and functional comparison between Hepatitis C Virus JFH1 and J6
Caillet-Saguy C.
PO-SI-06
Structure-function Study
Aldolases: Catalysis and Drug-design
Coinçon M.
PO-SI-07
Glycosylases
of
Metal-dependent
Fructose-1,6-bisphosphate
5-hydroxy-5-methylhydantoin DNA Containing Lesion: a Molecular Trap for DNA
Coste F.
PO-SI-08
Structure Analysis of Group I Plant Nucleases
Dohnalek J.
PO-SI-09
Crystal Structure of a Nucleocapsid-like Nucleoprotein-RNA Complex of Respiratory
Syncytial Virus
Duquerroy S.
PO-SI-10
BARF1
Structural Basis of Targeting of the Human Cytokine CSF-1 by the Viral Oncoprotein
Elegheert, J.
PO-SI-11
Structural Insights and ab initio Sequencing within the DING Proteins Family
Elias M.
PO-SI-12
Time-Resolved Crystal Structure Analysis of Mn(II) Dependent Hydrolysis
Reaction of ADP-Ribose Pyrophosphatase from Thermus thermophilus
HB8
Furuike Y.
PO-SI-13
Conformational Changes of Hepatitis C Virus Polymerase in Solution: Opening and
Closure, or SAXS Artifact?
Harrus D.
PO-SI-14
The Structural Chemistry and Structural Biology of Coloration in Marine Crustacea
Helliwell J.R.
PO-SI-15
The Ultra High-resolution X-ray Structure of Bovine H-protein at 0.79 Å; the Crystals
Grown in Microgravity Environment
Higashiura A.
Structural and Functional Study of a Vitamin D Receptor Mutant that Responds
PO-SI-16
Selectively to Gemini Ligands
Huet T.
PO-SI-17
Crystal Structure of the Glycosylated Form of Interleukin-23.
Ikemizu S.
PO-SI-18
Structural and Functional Studies of the Sarcoplasmic Reticulum Ca2+-ATPase
Jurková I.
PO-SI-19
Structural Basis for Histidine Mediated Vitamin D Receptor Agonistic and
Antagonistic Mechanisms of (23S)-25-dehydro-1a-hydroxyvitamin D3
26,23-lactone
Kakuda S.
PO-SI-20
X-ray Crystal Structure Analysis of Reaction Intermediate of Copper Amine
Oxidase from Arthrobacter globiformis
Kataoka M.
PO-SI-21
A Preliminary Neutron Crystallography on the Trypsin-bovine Pancreatic Trypsin
Inhibitor Complex
Kawamura K.
PO-SI-22
Catalysis in the Nitrilase Superfamily Enzymes; a Catalytic Triad or Tetrad?
Kimani S.
PO-SI-23
Stability
Crossroads Structural Study of the Architecture of Rap1 Protein Involved in Telomere
LeDu M.H.
PO-SI-24
A Unique Secondary Structure Switch Controls Constitutive Gene Silencing by Retinoic
Acid Receptor
le Maire A.
PO-SI-25
Anesthetic Gas Xenon and Nitrous Oxide: Same Physiological Target and Same
Binding Site in Proteins
Marassio G.
Crystallographic Structure of a Ribosomal RNA Methylase Involved in Bacterial
PO-SI-26
resistance to Aminoglycosides
Mechulam Y.
PO-SI-27
Structure of Nicotinic Acetylcholine Receptor in the Desensitized States
Nishino Y.
PO-SI-28
Substituted Pyrazolopyrimidines as Potent, Selective and CNS Active Inhibitors of
CK1d and CK1e
Ohren J.F.
PO-SI-29
Purine Nucleoside Phosphorylase from Schistosoma mansoni in Complex with
Ribose-1-Phosphate
Pereira H.M.
PO-SI-30
The Intriguing Quinone Reducing Site (Qi) of the Cytochrome b6f Complex
Picot D.
PO-SI-31
Antigen
Structural Studies of Glycosidase with High Specificity Towards the Blood Group B
Ponchel G.
PO-SI-32
Azide and Cyanide Have Different Inhibition Modes in Urate Oxidase
Prangé T.
PO-SI-33
Crystal Structure of Stable Protein, CutA1, from a Psychrotrophic
Bacterium Shewanella sp. SIB1
Takano K.
PO-SI-34
Structural Studies of Transcription Factor IIIC
Taylor N.M.I.
PO-SI-35
Three-dimensional Structure of HSP104, a Yeast Prion-remodeling Machine
Tsai F.T.F.
PO-SI-36
Scattering
Kinetics of Hepatitis B Virus Core Assembly by Time-resolved Solution X-ray
Tsuruta H.
PO-SI-37
Structural Studies of MobR, the 3-hydroxybenzoateresponsive
Transcriptional Regulator for the 3-hydroxybenzoate Hydroxylase Gene of
Comamonas Testosteroni KH122-3s
Yamaguchi H.
PO-SI-38
NADPH-free and -bound Structures of Conjugated Polyketone Reductase
C2 (CPR-C2) from Candida Parapsilosis IFO 0708
Yamamura A.
PO-SI-39
Water-mediated Crystal Transformations of D-xylose Isomerase and Thaumatin
Yamamura S.
PO-SI-40
Decreased Particle Size Explains Improved Protein Yield in E.coli
expressed Manihot esculenta Hydroxynitrile Lyase Mutants
Yamane T.
PO-SI-41
The Structural Basis of Serpin Polymerization
Yamasaki M.
PO-SI-42
ATPase
Structural Insights into the High Affinity Binding of Cardiac Glycosides to the Na+,K+Yamane L.
Poster Presentations
SESSION II
PO-SII-01
Biomolecular Solution X-ray Scattering at the National Synchrotron Light Source
Allaire M.
PO-SII-02
UV-based Automated Crystal Centering
Chavas Léonard M.G.
PO-SII-03
High Pressure Cryocooling and Microcrystallography @ MacCHESS
Englich U.
PO-SII-04
A New Paradigm for Macromolecular Crystallography Beamlines
Fourme R.
PO-SII-05
Interactions of Biomacromolecules - Protein Surface Modifying Agents in Protein
Crystallization
Hašek J.
PO-SII-06
Upgrade of High-throughput Sample Exchange Robots PAM and
Development of PAM/SPACE Reversible Cassettes
Hirakia M.
PO-SII-07
High-precision and High-throughput Diffractometer System for
Macromolecular Crystallography Beamlines at the Photon Factory
Igarashi N.
PO-SII-08
Applications.
The Cd-Te XPAD3S, a 2D Photon Counting Detector for High-Energy
Medjoubi K.
PO-SII-09
Synchrotron Radiation Beamline for Macromolecular Assemblies Operated by the
IPR at SPring-8
Nakagawa A.
PO-SII-10
Correlated Single-crystal Spectroscopy and X-ray Crystallography at Beamline
X26-C of the NSLS
Orville A.M.
PO-SII-11
Research and Development of the Electron Beam Focusing System for a High
Brightness X-ray Generator
Sakai T.
PO-SII-12
The Role of Modern Home-lab X-ray Systems in Increasing Beamline Efficiency
Smith V.
PO-SII-13
Improvement of Crystal Quality of Hematopoietic Prostaglandin D Synthase
with Novel Inhibitors in Microgravity Environment
Takahashi S.
PO-SII-14
Lanthanoid Complexes as New Tools for X-ray Structure Determination of Biological
Macromolecules: Application to Large Protein Complexes and Membrane Proteins.
Talon R.
PO-SII-15
Use of a 3 Circle Kappa Goniometer Goniostat for Structure Determination Using
Anomalous Diffraction on PROXIMA 1.
Thompson A.
PO-SII-16
Automation at SLS Protein Crystallography Beamline X06DA: from
Crystallization to Structure Determination
Wang M.
PO-SII-17
Semi-automated Protein Crystal Mounting Device for S-SAD Phasing and its Loop
Implementation Tool
Watanabe N.
PO-SII-18
Fully Automated Data Collection and Processing System in the Photon Factory
Macromolecular Crystallography Beamlines
Yamada Y.
PO-SII-19
The Structural Basis of Serpin Polymerization
Yamasaki M.