Shanghai Yaxin Biotechnology Co.,Ltd.
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Sequencing Grade Carboxypeptidase B
DESCRIPTION
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of
polypeptides. The molecular weight is 34,500 daltons, the pH optimum is 8.0, and pI is 6.0. Carboxypeptidase B is competitively
inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant
Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme
(such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.
MAIN FEATURES
Source
E. Coli
Purified by
HPLC
Physical form
Lyophilized powder
Specific activity
NLT 200 units/mg pro
Contaminant activity
No trypsin, chymotrypsin, carboxypeptidase A, or other proteases contaminant.
APPLICATION
Protein structure and sequence analysis, such as hydrolyze basic amino acids lysine, arginine and histidine from the C-terminal end
of polypeptides.
Antibody quality control.
RECOMMEND USAGE
To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.5. The ratio to aimed protein is 1:50 to 1:1000
(w/w), the optimum pH is pH7-9.
STORAGE AND STABILITY
Recommend recombinant carboxypeptidase B lyophilized powder stored under 2-8℃ in sealed container. It is stable within 24
months.
After dissolved, it should be stored under -20℃, It is stable within 24 months and no activity lose after 10 times repeated freezing
and thawing.
RELATED PRODUCT
Recombinant Trypsin (sequencing grade);
Recombinant Carboxypeptidase B (sequencing grade);
Recombinant Chymotrypsin (sequencing grade);
V8 (sequencing grade).
No.466 YinDu Road,XuHui, Shanghai, China.
http://www.yaxinbio.com, email: sales@yaxinbio.com