Recombinant Carboxypeptidase B
Animal Origin Free, No Virus Contaminant
DESCRIPTION
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and
histidine from the C-terminal end of polypeptides. The molecular weight is 34,500 daltons,
the pH optimum is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by
arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA.
Recombinant Carboxypeptidase B (EC 3.4.17.2) is expressed in E.Coli and purified by
high pressure liquid chromatography. There is no trace of other enzyme (such as
carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are
present in the preparation.
ADVANTAGES
Animal origin free
YaxinBio recombinant carboxypeptidase B belongs to the AOF level 3. Level 3 recombinant
carboxypeptidases B eliminate the risk of virus presence, or of any other potential adventitious agents
found in animal-derived carboxypeptitase B.
Stable
A sterile recombinant carboxypeptidase B lyophilized powder eliminates the risk of contamination and
decreases the chances of activity loss in the process of transport and storage. It is also very convenient
for transport and storage.
High purity
1) Recombinant carboxypeptidase B provides increased specificity and eliminates contaminating
activities found in enzymes with lower purity level.
2) No other contaminating proteases such as chymotrypsin and carboxypeptidase A. Less than 10ppm of
recombinant trypsin.
3) One single main band at 35kD by SDS-PAGE.
MAIN FEATURES
Source
E. Coli
Purified by
HPLC
Physical form
White or white-like lyophilized powder
Additives
Tris salts, NaCl salts and carbohydrates.
Protein content
35% ～ 50%
Specific activity
>170 units/mg pro.
Purity
> 95%
Contaminant activity
No chymotrypsin, carboxypeptidase A, and other protease contaminant.
Less than 10ppm of recombinant trypsin.
Figure 1. SDS Page of YaxinBio Recombinant Carboxypeptidase B (CPB)
PRODUCT INFORMATION
Product
Cat. No.
Specific activity
Packaging
Manufacturer
Recombinant
RCPB01170
≥170 u/mg pro
10mg bulk
YaxinBio
Carboxypeptidase B
RCPB01100
≥100 u/mg pro
1g bulk
YaxinBio
UNIT DEFINITION：One Unit of carboxypeptidase B activity hydrolyzes one micromole of hippuryl-L-arginine per minute at 25℃, pH 7.65.
RELATED PRODUCT: Recombinant Porcine Trypsin