MINNESOTA MASS SPECTROMETRY DISCUSSION GROUP
SEPTEMBER 22, 2008
Minn Mass Media
Fall Meeting 2008
Professor Joseph A. Loo, Monday September 22nd
“Charge On, Charge Off:
Bioanalytical Applications of Multiple Charging and Mass Spectrometry”
There will be a Fall Meeting
of
t h e M i n n e s o t a Ma s s
Spectrometry Discussion Group
on Monday September 22nd at
The University of Minnesota:
Food Science and Nutrition
Room 23
1334 Eckles Avenue
St. Paul, MN 55108
The speaker will be Professor
Joseph A. Loo of the Department
of Chemistry and Biochemistry
and the Department of Biological
Chemistry, David Geffen School
of Medicine at the University of
California, Los Angeles
Pizza and Beverages will be
provided and will be available at
6:30 pm, and the presentation
will begin at 7:00.
Please RSVP with number
of attendees to
minnmass@mac.com
PAGE 1 of 4
MINNESOTA MASS SPECTROMETRY DISCUSSION GROUP
SEPTEMBER 22, 2008
ABSTRACT
The characterization of large biomolecules such as proteins by mass spectrometry was greatly facilitated
by the development of desorption/ionization methods, such as matrix-assisted laser desorption/ionization
(MALDI) and electrospray ionization (ESI). One of the hallmark features of ESI is the multiple charging
phenomenon that allows large molecules to be measured using analyzers of moderate mass-to-charge ratio
(m/z). Multiple charging allows also efficient dissociation of intact proteins (i.e., top-down mass
spectrometry) for sequencing, identification, and detection of posttranslational modifications. New
ambient methods for multiple charging of proteins desorbed from solids potentially offers more efficient
means for protein identification from tissues. Increasing charging beyond that generated by normal ESI
processes can be exploited for the analysis of protein-ligand and protein-protein complexes. Reducing
multiple charging can be advantageous for the detection of very large proteins up to the MegaDalton size,
especially when coupled to ion mobility analyzers.
BIOSKETCH OF PROFESSOR JOSEPH A. LOO
Dr. Joseph A. Loo is a Professor in the Department of Biological Chemistry, David Geffen School of Medicine, and in the Department of Chemistry & Biochemistry at the University of California, Los Angeles
(UCLA), and he is the Faculty Director of UCLA Jonsson Comprehensive Cancer Center Mass Spectrometry and Proteomics Technology Center. He is also a member of UCLA/DOE Laboratory for Genomics and
Proteomics, the UCLA Molecular Biology Institute, and the UCLA Jonsson Comprehensive Cancer Center.
He is an expert in the mass spectrometry characterization of proteins and their post-translational modifications, and is the author of over 180 scientific publications. He has been on the Editorial Boards of the journals Bioconjugate Chemistry, Analytical Chemistry (A-Page), Journal of the American Society for Mass Spectrometry
(JASMS), and Chemical & Engineering News and currently he serves on the Editorial Advisory Board for Rapid
Communications in Mass Spectrometry and as an Associate Editor for JASMS. In 2000-2002, he served on
the Board of Directors for the American Society for Mass Spectrometry.
His research interests include the development of bioanalytical methods for the structural characterization
of proteins and their application for proteomics and disease biomarkers. Currently, his laboratory is developing technologies to profile proteins in human salivary fluids for their application for cancer biomarker discovery. He is also interested in the development and application of mass spectrometry for the study of
noncovalently-bound macromolecular assemblies and their interactions with other binding partners and
ligands. At UCLA, Dr. Loo teaches a course for undergraduate students on “Chemical Instrumentation” and
a graduate level course on “Proteomics and Mass Spectrometry.”
Before he joined UCLA in 2001, he was Group Leader of the Biological Mass Spectrometry and Proteomics
Teams at Parke-Davis Pharmaceutical (currently Pfizer Global Research), Ann Arbor, MI. He worked at
Warner-Lambert/Parke-Davis/Pfizer for nearly 10 years before moving to UCLA.
He received his BS degree in Chemistry from Clarkson University (Potsdam, NY), and his Ph.D. degree in
analytical chemistry from Cornell University in 1987 with Professor Fred W. McLafferty, working on the development of Fourier transform mass spectrometry for bioanalytical applications. He carried out postdoctoral research at Pacific Northwest National Laboratory, Richland, WA with Dr. Richard D. Smith on
the development of electrospray ionization mass spectrometry and capillary electrophoresis for protein
characterization.
PAGE 2 of 4
MINNESOTA MASS SPECTROMETRY DISCUSSION GROUP
SEPTEMBER 22, 2008
Map of St. Paul Campus Showing N Cleveland Avenue.
These maps are also available at the following URL:
http://www1.umn.edu/twincities/maps/FScN/index.html
Close-Up Map of Food Science
and Nutrition. Parking is
available in Lot S-101 as shown.
PAGE 3 of 4
MINNESOTA MASS SPECTROMETRY DISCUSSION GROUP
TALK FACILITIES
University of Minnesota - Food
Science and Nutrition Building
T h e Fo o d S c i e n c e a n d
Nutrition Building is Located On
t h e S t . Pa u l C a m p u s o f t h e
University of Minnesota. See the
map on the previous page for
details or email:
minnmass@mac.com
for clarification.
MinnMass
Corporate Sponsors
AGILENT
APPLIED BIOSYSTEMS
BRAUN INTERTEC
BRUKER DALTONICS
CARGILL
SEPTEMBER 22, 2008
Calendar
September 22nd, 2008
MinnMass Fall Meeting
Professor Joseph Loo
Talk Title: Bioanalytical Applications of
Multiple Charging and Mass
Spectrometry
Location: University of Minnesota
Winter Meeting
TBA
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PAGE 4 of 4