Part 1
Genei
New Products
Plant Direct GenAmp PCR Kit
Mix is tested for detecting different subtypes of Human Papilloma virus(HPV)
using consensus primers.
Introduction:
DNA extraction and purification from plants requires large amounts of
starting material (e.g. Leaf) and adds time and expense to PCR protocols, as
well as increases the risk of cross contamination and human error.
Description:
The Plant Direct GenAmp PCR Kit is fast, easy to perform and consequently
ideal for large sample numbers. In addition, only a very small sample (0.5 cm
leaf punch, for example) is enough for the assay.
The protocol involves direct PCR amplification from the plant material
without a DNA extraction step. The leaf material is crushed in one buffer,
followed by dilution and incubation in a second buffer.
The Plant Direct GenAmp PCR Kit represents a considerable simplification of
present approaches by eliminating DNA isolation prior to PCR amplification.
Highlights:
This kit is useful in plant studies and downstream applications like High
throughput plant genomic PCR, Phylogenetic or population-based plant
DNA barcoding, plant seed fidelity testing etc. It is also suitable for DNA
amplification across a broad range of plant species (Refer Fig. 2 and 3).
Time Duration: Hands on time: ~20 minutes.
Ordering Information:
610681300011730
KT234
610681300021730
Product
Plant Direct GenAmp PCR Kit,
(50 reactions)
KT222C Plant Direct GenAmp PCR Kit,
(250 reactions)
Unit Price
`
4000
15000
Description:
The HotStart PCR Master Mix (2X)is a premixed, ready to use solution
containing HotStart Taq DNA Polymerase, dNTPs, standard reaction buffer,
stabilizers and an easy to track blue loading dye. Inclusion of tracking
dye allows direct loading & analysis of PCR products by agarose gel
electrophoresis following PCR amplification. The Ready to use PCR mix
provides robust, reliable & consistent performance for PCR applications
where specificity & sensitivity are desired. The HotStart PCR Master Mix is
supplied at 2X concentration to accommodate the addition of Magnesium
chloride, additives like BSA, DMSO, glycerol etc.
Storage:-20 0C.
Performance Test:
The HotStart PCR Master Mix (2X) is tested extensively for its reproducible
performance in critical PCR amplifications.
Mix is tested for amplification of 131 bp fragment of TNF gene and 400 bp
fragment of β-Globin gene from human genomic DNA using non-optimal
primers.
www.merckmillipore.com
Cat No.
PI. No.
Product
610604400021730
MME44 GeNei™ HotStart Blue Dye
PCR Master Mix (2X), 1 X
100 reactions
Unit Price
`
4,630
NanoGreen DNA Elpho Buffer is a safe alternative to Ethidium bromide. It can
be used as a component of gel and electrophoresis buffer to visualise nucleic
acids. The DNA bands appear green under standard UV transilluminator. The
band of interest can be excised from the gel and further purification can be
achieved by standard methods of nucleic acid purification.
NanoGreen DNA Elpho Buffer is supplied in 50X TAE buffer. Dilute 50 fold
with autoclaved water.
Storage: Room Temperature, Protected from Light.
HotStart Blue Dye PCR Master Mix
(2X)Product
162
Ordering information:
Easy, Economical & Environmentally safe
Applications:
PI. No.
• Saves time-directly from PCR to gel electrophoresis.
• Room temperature assembly.
• Higher sensitivity & specificity compared to conventional Taq DNA
Polymerase.
• Efficient amplification for as low as 25µl reaction volume.
NanoGreen DNA Elpho Buffer
• Excludes the need for DNA extraction.
• Fast and simple protocol requiring minimal amount of sample.
• Ideal for large number of samples.
• Tested with a wide variety of plant species
Cat No.
Highlights:
Bioscience
Ordering information:
Cat No.
PI. No.
Product
612602584001730
FC101M NanoGreen DNA Elpho
Buffer, 400 ml
Unit Price
`
5,120
GeNei™ Direct PCR Kit (for Mammalian Cell
lines, Bacteria and Yeast)
Isolation of PCR grade DNA from various samples is time consuming and
not easily adaptable to automation. To save on time, there is a need to
obviate DNA extraction from the samples prior to PCR. The ever increasing
use of PCR in clinical and research applications, requires a rapid, simple and
an effective method of sample processing. The GenAmp PCR Direct Kit for
Mammalian Cell lines, Bacteria and Yeast enables DNA amplification directly
from mammalian cells, bacteria and yeast samples without any prior DNA
extraction. The protocol is fast and easy, without the need for steps like
phenol extraction, DNA precipitation and column purification.
Highlights:
•
•
•
•
Direct amplification of genomic DNA from various sources like bacteria,
yeast and mammalian cells.
Use of Single buffer.
Consistent and highly reproducible results.
Fast and easy protocol - Eliminates need for a separate DNA extraction
step prior to PCR and enables rapid extraction of DNA from the samples.
Availability of the products that of animal origin is subject to government regulations.
Please check with your local MM representative before ordering.