Marketplace
savings
25% OFF
Thermo Scientific GeneJET
DNA / RNA Purification Kits
• NEW Kits for Plasmid
Midi and Maxipreps
• NEW Kits for Blood and Plant
Samples
Thermo Scientific Modifying
Enzymes
Thermo Scientific TurboFect
Transfection Reagent
Thermo Scientific Phusion
High-Fidelity
DNA Polymerase
Products
Thermo Scientific
Maxima and
RevertAid Reverse
Transcription
Products
Thermo Scientific
Molecular Biology Marketplace.
A quarterly publication containing special offers
for significant savings on a variety of
molecular biology products.
FMW13
Thermo Scientific GeneJET Nucleic Acid
Purification Kits - 25% Discount
Nucleic Acid
Purification
Plasmid
DNA
Miniprep
25% OFF
Genomic
DNA
Plant
DNA
Midiprep Maxiprep
Blood
DNA
DNA
Fragments
Total RNA
DNA from
Cells &
Tissues
Plant
RNA
Blood
RNA
RNA from
Cells &
Tissues
From PCR From Gels
High Yield of Purified Plasmid DNA
GeneJET Plasmid Miniprep Kit
GeneJET Plasmid Midiprep Kit
GeneJET Plasmid Maxiprep Kit
High quality plasmid DNA: A260/280 1.7 – 1.9.
High yields and high concentration of purified DNA.
• Miniprep – 20 μg, ≥ 400 ng/μl
• Midiprep – 200 μg, ≥ 400 ng/μl
• Maxiprep – 750 μg, ≥ 400 ng/μl
Silica-based membrane technology.
Centrifuge and vacuum manifold protocols.
Purified DNA is ideal for transfection of robust cell lines.
Centrifuge/Vacuum Protocol
Pelleted bacteria
Pelleted bacteria
Alkaline lysate
Alkaline
lysate
Clear lysate by
centrifugation
Clear lysate by
centrifugation
Bind DNA
Bind
DNA
Consistent and Reproducible Miniprep Yields
Relaxed
Supercoiled
Wash
Wash
M
1
2
3
4
5
6
Elute
Elute
A pBluescript-based high copy number plasmid was purified
from E. coli culture with 6 different GeneJET Spin Columns.
M – FastRuler DNA Ladder, High Range, ready-to-use (#SM1123)
1-6 – plasmid DNA isolated with 6 different GeneJET Spin Columns
Promo
Code
Cat. No.
Product
MPD32
K0503
K0502
GeneJET Plasmid Miniprep Kit
250
50
NEW MPD32
K0482
K0481
GeneJET Plasmid Midiprep Kit
100
25
NEW MPD32
K0492
K0491
GeneJET Plasmid Maxiprep Kit
25
10
Preps
Please quote promotion code MPD32 to take advantage of this offer. Offers are valid until January 31st, 2012.
Thermo Scientific Nucleic Acid Purification
25% OFF
Efficient genomic DNA purification
GeneJET Genomic DNA and RNA kits are compatible with a wide variety of samples:
• Fresh or frozen
GeneJET Genomic DNA Purification Kit
• Purify RNA-free genomic DNA from a wide variety of tissues or cells in only 20 minutes
GeneJET Whole Blood Genomic DNA Purification Mini Kit
• Purify RNA-free genomic DNA
• Fast 20-minute procedure with no separate cell lysis step
GeneJET Plant Genomic DNA Purification Kit
• Purify DNA without column-clogging lysate filtration steps
• Material-specific protocols provided
• Anticoagulants: EDTA,
citrate, heparin
Spin columns based on silica-membrane technology.
Specialized and detailed protocols for different sample types.
Purified DNA can be used in a wide range of downstream applications.
• Various plant tissues:
Leaves, roots, seeds,
pine needles, etc.
Promo
Code
Cat. No.
Product
MPD32
K0722
K0721
GeneJET Genomic DNA
Purification Kit
250
50
NEW
MPD32
K0782
K0781
GeneJET Whole Blood Genomic
DNA Purification Mini Kit
250
50
NEW
MPD32
K0792
K0791
GeneJET Plant Genomic DNA
Purification Kit
250
50
• Buffy coat
• Bone marrow
• Body fluids (urine)
• Multiple animal species
• Multiple plant species
Preps
Please quote promotion code MPD32 to take advantage of this offer. Offers are valid until January 31st, 2012.
25% OFF
High purity total RNA isolation
GeneJET RNA Purification Kit
• Purify high integrity (RIN > 8.0), DNA-free total RNA from a wide
variety of sources
GeneJET Whole Blood RNA Purification Mini Kit
• Purify DNA-free RNA with no DNase step
• High RNA integrity (RIN > 8.0)
GeneJET Plant RNA Purification Kit
• Purify DNA-free RNA with no DNase step
• High RNA integrity (RIN > 8.0)
Spin columns based on silica-membrane technology.
Specialized and detailed protocols for different sample types.
Purified RNA can be used in a wide range of downstream applications.
Promo
Code
Cat. No.
Product
MPD32
K0732
K0731
GeneJET RNA Purification Kit
250
50
NEW
MPD32
K0761
GeneJET Whole Blood RNA
Purification Mini Kit
50
NEW
MPD32
K0802
K0801
GeneJET Plant RNA
Purification Kit
250
50
Preps
Please quote promotion code MPD32 to take advantage of this offer. Offers are valid until January 31st, 2012.
25% OFF
Effective DNA fragment purification
GeneJET PCR Purification Kit
• DNA recovery up to 100% (25 bp – 20 kb)
• Effective removal of primers, unincorporated nucleotides, enzymes and salts
GeneJET Gel Extraction Kit
• DNA recovery up to 95% (25 bp – 20 kb)
• Purify DNA fragments from standard or low-melt agarose gels run with TAE or TBE buffer
Promo
Code
Cat. No.
Product
MPD32
K0702
K0701
GeneJET PCR Purification Kit
250
50
MPD32
K0692
K0691
GeneJET Gel Extraction Kit
250
50
Preps
Please quote promotion code MPD32 to take advantage of this offer. Offers are valid until January 31st, 2012.
Superior Transfection Efficiency With Minimal Toxicity
25% OFF
Thermo Scientific TurboFect Transfection Reagent
Benefits
• High transfection efficiency of a wide variety of cell types, including
primary, differentiated and undifferentiated cells
• Transfection can be performed in the presence or absence of serum
• Demonstrates superior transfection efficiency and minimal toxicity when
compared to lipid-based or other polymer-based transfection reagents
• Simple 3-step transfection protocol compared with 6-7 steps for other
transfection reagents
Triple transfection of HeLa cells using TurboFect™
eGFP
Cells successfully transfected with TurboFect Transfection Reagent
eCFP
Permanently growing cell lines:
• B50
DsRed
• BHK21
Primary cell cultures:
• Calu1
• Human lung fibroblasts
• CHO
• Rat fibroblasts
• COLO
• Mouse bone marrow derived
• Cos-7
dendritic cells
HeLa cells were co-transfected with 1:1:1 mixture
• HEK293
• Mouse bone marrow derived
of plasmids encoding for eGFP (cytoplasm), eCFP
• HeLa & HeLa S3
(nucleus) and DsRed pro (membrane).
macrophages
• Hep2C & HepG2
• Mouse lymphoma primary cell culture
• Jurkat
• Primary human muscle cells
• L929
• RAW264
• MDCK
Promo
Cat. No.
Description
Quantity
• NIH3T3
Code
• Sp2/Ag14
MPD32
R0531
TurboFect Transfection Reagent
1.0 ml
• WEHI
Please quote promotion code MPD32 to take advantage of this offer. Offers are valid until January 31st, 2012.
• Raji
Thermo Scientific Modifying Enzymes
RiboLockTM RNase Inhibitor – Lock your RNase away!
• Inhibits the activity of eukaryotic RNases A, B and C
• Protects RNA from degradation at temperatures up to 55°C
• Active at pH 5-9 in a wide range of reaction conditions
25% OFF
Inhibition of RNase A with
RiboLock RNase Inhibitor
Protected
human RNA
DNase I – RNase Free!
• Used to prepare DNA-free RNA
• Removal of template DNA following in vitro transcription
• Digests single and double-stranded DNA
FastAPTM Thermosensitive Alkaline Phosphatase –
Fast Dephosphorylation!
• Fast dephosphorylation – 10 minutes at 37°C for all types of DNA ends
• Fast and complete inactivation – 5 minutes at 75°C
• Simultaneous digestion and dephosphorylation of vector DNA
• 100% active in restriction enzyme and PCR buffers
Promo
Code
Cat. No.
Product
MPD32
EO0381
EO0382
EO0384
RiboLock RNase Inhibitor
2,500 u, 40 u/µl
10,000 u, 40 u/µl
60,000 u, 40 u/µl
MPD32
EN0521
EN0523
EN0525*
DNase I, RNase-free
1000 u, 1 u/µl
1000 u, 50 u/µl
1000 u, 1 u/µl
MPD32
EF0651
EF0652
FastAP Thermosensitive
Alkaline Phosphatase
1000 u, 1 u/µl
5 X 1000 u, 1 u/µl
M
C
1
2
3
4
Increasing
amounts of
RNase A
Total human RNA (1 μg) was incubated at 37�C with 20 u
of RiboLock RNase inhibitor and increasing amounts of
RNase A (#EN0531).
M – RiboRuler High Range RNA Ladder (#SM1821)
C – total human RNA and RNase A (no RiboLock added)
1-4 – total human RNA, RiboLock and increasing amounts of RNase A
Size
*supplied with MnCl2
Please quote promotion code MPD32 to take advantage of this offer. Offers are valid until January 31st, 2012.
Phusion – PCR performance no other enzyme can match
Thermo Scientific Phusion High-Fidelity DNA Polymerase products
25% OFF
Phusion® DNA Polymerases are the established standard for high-fidelity PCR. The extreme accuracy of Phusion
High-Fidelity DNA Polymerase is available for you in several formats. Just pick the one that best suits your needs
and make no compromises. Phusion is the superior solution for:
• High-Fidelity PCR - molecular cloning
• Hot Start PCR - allows increased specificity and reaction setup at room temperature
• Fast PCR - enables short reaction times
• RT-PCR - complete 2-step kit designed for high-fidelity RT-PCR
• Site-Directed Mutagenesis - versatile and efficient tool for introducing point mutations, insertions and deletions
in any type of plasmid DNA
Promo
Code
Cat. No
Description
Quantity
MPD32
F-530L
F-530S
Phusion High-Fidelity DNA Polymerase
500 u
100 u
MPD32
F-549L
F-549S
Phusion Hot Start II High-Fidelity DNA
Polymerase
500 u
100 u
MPD32
F-531L
F-531S
Phusion High-Fidelity PCR Master Mix
with HF Buffer
500 x 50 µl
100 x 50 µl
MPD32
F-532L
F-532S
Phusion High-Fidelity PCR Master Mix
with GC Buffer
500 x 50 µl
100 x 50 µl
MPD32
F-553L
F-553S
Phusion High-Fidelity PCR Kit
200 x 50 µl
50 x 50 µl
MPD32
F-548L
F-548S
Phusion Flash High-Fidelity PCR Master Mix
500 x 20 µl
100 x 20 µl
MPD32
F-541
Phusion Site-Directed Mutagenesis Kit
20 rxns
MPD32
F-546L
F-546S
Phusion RT-PCR Kit
100 rxns
20 rxns
Please quote promotion code MPD32 to take advantage of this offer. Offers are valid until January 31st, 2012.
Less enzyme - superior yield
Pfu
(5 U)
modified Pfu
(2.5 U)
Phusion DNA
(1 U)
A 3.8 kb fragment from the human beta
globin gene was amplified with three
different DNA polymerases. Phusion
DNA Polymerase was able to amplify
the 3.8 kb genomic fragment with a
combined annealing and extension step
of only 1 minute, thus being significantly
faster than the two other polymerases
tested. A single unit of Phusion DNA
Polymerase produced higher yields
than 2.5 or 5 units of the Pfu
DNA polymerases.
Interested in improving your SNP genotyping results?
Get a free sample pack of the new Thermo Scientific DyNAmo SNP Genotyping
Master Mix (60 x 25 µl rxns). Find out more and request your free sample at
www.thermoscientific.com/snpgenotyping
Thermo Scientific PCR and qPCR
FREE SAMPLE
For efficient and convenient reverse transcription
Thermo Scientific Maxima and RevertAid reverse transcription products
Our selection of Thermo Scientific reverse transcriptases includes both genetically modified
M-MuLV RT enzymes as well as those obtained through in vitro evolution of M-MuLV RT,
offering a wide range of RT enzymes designed for routine to enhanced performance.
25% OFF
Optimal reaction
temperature
Active
up to
Reading
length
RNase H
activity
Amount of total
RNA/
20 μl reaction
cDNA labeling
for microarray
cDNA
labeling
Primer
extension
Maxima® Reverse Transcriptase
50 - 55°C
65°C
20 kb
+
0.01 ng - 5 μg
••••
••••
••
RevertAid™ Premium Reverse
Transcriptase
50 - 55°C
60°C
20 kb
–
0.01 ng - 5 μg
•••••
•••••
•••••
RevertAid™ H Minus Reverse
Transcriptase
42 - 45°C
55°C
13 kb
–
0.1 ng - 5 μg
•••
•••
•••••
42°C
50°C
13 kb
+
0.1 ng - 5 μg
••
••
•
Reverse transcriptase
RevertAid™ Reverse Transcriptase
Promo
Code
Cat. No
Description
Quantity
MPD32
K1641
K1642
Maxima First Strand cDNA Synthesis Kit
50 rxns
200 rxns
MPD32
EP0741
EP0742
EP0743
Maxima Reverse Transcriptase
2000 u, 200 u/µl
10000 u, 200 u/µl
4x10000 u, 200 u/µl
MPD32
K1651
K1652
RevertAid Premium First Strand
cDNA
Synthesis Kit
20 rxns
100 rxns
MPD32
EP0731
EP0732
EP0733
RevertAid Premium Reverse Transcriptase
2000 u, 200 u/µl
10000 u, 200 u/µl
4x10000 u, 200 u/µl
MPD32
K1631
K1632
RevertAid H Minus First Strand
cDNA Synthesis Kit
20 rxns
100 rxns
MPD32
EP0451
EP0452
RevertAid H Minus Reverse Transcriptase
10000 u, 200 u/µl
5x10000 u, 200 u/µl
MPD32
K1621
K1622
RevertAid First Strand cDNA
Synthesis Kit
20 rxns
100 rxns
MPD32
EP0441
EP0442
RevertAid Reverse Transcriptase
10000 u, 200 u/µl
5x10000 u, 200 u/µl
Please quote promotion code MPD32 to take advantage of this offer.
Offers are valid until January 31st, 2012.
Reproducible RT-qPCR results using the
Maxima First Strand cDNA Synthesis Kit
First strand cDNA was generated from 100 ng
-1 pg of total Jurkat cell RNA with the Maxima
First Strand cDNA Synthesis Kit in 16 replicate
reactions. Synthesized cDNA was used as a
template in subsequent qPCR with Maxima SYBR
Green/ROX qPCR Master Mix on the ABI 7500
Real-Time PCR instrument. Parallel RT reactions
demonstrate reproducible cDNA synthesis and low
variability levels with a wide range of starting RNA
amounts.
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