ADULT STEM CELLS
Types of Adult
Stem Cells
1. AUTOLOGOUS
 From the patient back
to the patient
4
MSC’s per Marrow Cells
MSCs DECLINE WITH AGE
1/10,000
Estimates
obtained by
CFU-f assay
Age (years)
Arnold I. Caplan | Adult Mesenchymal Stem Cells for Tissue Engineering Versus Regenerative Medicine | Journal of Cellular Physiology 2007.
5
ADULT STEM CELLS
Types of Adult
Stem Cells
1. AUTOLOGOUS
 From the patient back
to the patient
2. ALLOGENEIC
 From donor to patient
 Source from licensed
tissue bank
EMBRYONIC
STEMEDICA
6
ADVANTAGE MULTIPLE CELL PRODUCTS
Sources
of Cells
Ischemic Tolerant
Stem Cells
Master
Banks
IMPORTANT
Treatment of
complex medical
conditions
often
st
1
requires multiple
stem cells
isolated from
specific tissues
to maximize
efficacy
itNSC’s
Donated Brain Tissue
itMSC’s
Donated Bone Marrow
Donated Eye
Tissue
Live Cell Supernatant
itRPE’s
Derived Factors
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Spinal Cord Injury – Incomplete Quadriplegic
Prior to stem cell therapy, she could not:
Stand,
Walk,
Brush her hair,
Change her clothes,
Bathe,
Feed herself,
Control her bowel & bladder,
Get out of bed by herself,
Feel anything below her neck,
Be rolled over every three hours,
Spinal Cord Injury – Incomplete Quadriplegic
After stem cell therapy, she could:
Stand,
Walk with assistance,
Brush her hair,
Change her clothes,
Bathe,
Feed herself,
Control her bowel & bladder,
Get out of bed by herself,
Feel her legs and feet,
Take care of herself,
OREST – TRAUMATIC BRAIN INJURY
 Hit by car -- Diagnosed – 2 neuro
surgeons & 4 neurologist - Diffuse
axonal injury. Most traumatic –
wide spread brain injury –UCLA, Loma
Linda, Kaiser, Northridge
 Parents told to give up all hope of
recovery
 Thought to be in pain – maybe
better if he had died in accident
Vegetative State
Completely non responsive
Complete paralysis - quadriplegic
Unable to feed himself
Click to Play
No bowel or bladder control
Significant spasticity
Emergency FDA IND approved
OREST – TRAUMATIC BRAIN INJURY
Recovered from vegetative State within
ONE week of stem cell treatment
Cards games
with Grandpa
 Completely responsive - cognitively
& physically
 Able to feed himself within ONE
week of treatment
 Bowel or bladder control restored
within two weeks of treatment
 Back in school – fully caught up on
subjects
Back to the physical activities he enjoys
Computer Games
with Dr. Levy
BRAIN STEM STROKE
Patient Two
Click to
Play
Brain Stem Stroke
 Less than seven months to
live
 Food pureed & G Tube
delivered
 Aphasic – without speech
 “Locked In” syndrome
 Incontinent – Completely
Paralyzed
 Less than 97 pounds
ISCHEMIC BRAIN STEM STROKE
 Back riding his horse
 Walking with assistance
 Eating by himself
 Gained 47 pounds within first
five months
 Bowel & bladder control
returned
 Regained speech
 Interacting socially
Case One – Chronic Stroke Patient
VIDEO
Stroke
PRIOR TO TREATMENT
Patient
One
Right sided paralysis
Aphasic - without speech
 Major spasticity with leg drag and arm
clenched to chest
Incontinent
Suffered from depression
Required 24/7 caretaking
6 years of extensive PT with min response
48
JOHN BRODIE
AFTER STEM CELL
TREATMENTS




Speech returning
Completely independent
Normal walking
Return to golfing & full
activities of daily living
 Travels globally
 85% relaxation of paralyzed
arm
49
K2 SCAR TREATED WITH STEM CELLS & LASER
MEET DEBBIE
Before Treatment
After Treatment
61
SKIN CONSTRUCT CLINICAL TRIAL – BURNS
Before
Treatment
3rd Degree
IMMEDIATE
BARRIER
PROTECTION
SKIN CONSTRUCT CLINICAL TRIAL – BURNS
Before
Treatment
3rd Degree
REDUCED SCARRING,
MINIMIZED
CONTRACTURE,
RESTORE RM
15 months
Patient Three – Brain Stem Stroke
Patient Three
Brain Stem Stroke
Less than a few months to live
Quadriplegic
Food pureed & G Tube delivered
Aphasic – without speech
Tracheotomy – 2 liters of fluids
each night from lungs
Incontinent – constant urinary
infections
 Less than 85 pounds
Patient Three – Brain Stem Stroke
 Can curl a 40 pound weight with
previously paralyzed arm
 Walking with walker & eating by self –
no more drooling or bibs
 Tracheotomy removed
 Bowel & bladder control restored
 Speech restored
 No more lung infections or secretions
GLOBAL PROBLEM -- TBI
After Injury - Before Stem Cells
 Near blind in both eyes - cracked glass vision
in one eye
 Hearing loss left ear – Severe Tinnitus
 Excruciating pain in left arm
BEFORE INJURY
 No feeling in left side of face
“A bullet struck him below the  Impaired cognitive processing
right eye, exited near his left ear
and damaged the front of his  9 morphine pills a day
brain."
 Suicidal – daily ideation
 PTSD – anger outbursts-rage-resentment
 Low energy levels – no motivation – self pity
 Could not stay at home alone – last 8 years
AFTER INJURY
 Metal plate covers ½ of head. Loss of 1/3 of
his frontal cortex
GLOBAL PROBLEM -- TBI
Six Weeks After Stem Cells
 Near blind in both eyes- cracked glass vision one eye – unable to read
 Significant improvement in clarity so he is beginning to read - excited
 Hearing loss left ear – Severe Tinnitus
 Tinnitus disappeared
 Excruciating pain in left arm
 Pain completely gone
 No feeling in left side of face
 Feeling completely restored
 Impaired cognitive procession
 Quicker responses – no delays in word search
 9 morphine pills a day
 Reduced by six per day
 Suicidal – daily ideation
AFTER INJURY with
 None since 3 weeks after treatment
WIFE TONIA
 Low energy levels – no motivation – self pity
 Wakes up energized – grateful attitude – no more self pity
 Could not stay at home alone – last 8 years
 Stayed at home entire weekend by himself – doing painting & yard work
 PTSD – anger outbursts-rage-resentment
 Very few - very noticeable improvement
TRAUMATIC BRAIN INJURY
MATTHEW WRIGHT




At age 19 Suffered
major Traumatic Brain
Injury since 2009 when
struck on the back of
the head after coming
to the aid of a stranger
Cognition not affected
Locked in, quadriplegic,
aphasic, vision
impaired, breathing,
blood pressure
compromised, g-tube
fed, 8-12 aspirations
daily
Body temp unregulated
TRAUMATIC BRAIN INJURY







Able to propel his own
wheelchair
Regaining strength and
mobility
Able to stand on his
own
Making progress in
walking
No more aspirations
Regulated body
temperature
Speaking with closed
trach
JHOONAR BARRERA
Paraplegic since
September 2007
 Age 35
 9 years service with 82nd
Airborne Division
 Two tours of duty in
Afghanistan and Iraq
 After leaving Army
accident with heavy
equipment September
27,2007
 Spinal Cord Injury (T11L1)
149
PARAPLEGIC
 Very well known



businessman.
Paralyzed from the waist
down from accident that
broke his back.
Restricted to wheelchair
4 years
After 2nd treatment
TRANSLATIONAL MEDICINE TODAY
Significant enhancement: energy, strength,
libido, cognition, & stamina