HUMAN ANATOMY AND PHYSIOLOGY LAB WORKBOOK SEMESTER I Contents Anatomy Academy _______________________________________2 Microscope _____________________________________________3 The Cell ________________________________________________6 The Cell Cycle ___________________________________________ 9 Appendicular skeleton ____________________________________ 16 Axial Skeleton ___________________________________________35 Skull ___________________________________________________47 Muscular System _________________________________________56 Head and Torso Muscles ______________________________61 Upper Limb Muscles _________________________________65 Lower Limb Muscles __________________________________70 The Nervous System ______________________________________78 Spinal Nerves ________________________________________81 Spinal Cord __________________________________________93 Brain _______________________________________________98 Eye ________________________________________________ 109 Ear ________________________________________________122 Human Anatomy and Physiology Lab workbook Semester I 2nd Edition Somerset Community College Biology Faculty Elaine Kohrman – Editor Shawn Stratmann, Clint Hayes – Photos Rose Kohrman – Design 1 INTERACTIVE ACTIVITIES FOR THIS LAB BOOK CAN BE FOUND ON BLACKBOARD IN THE ANATOMY ACADEMY ECOMMUNITY. Log in to BlackBoard. Click on the eCommunity tab at the top of the page. Search for ‘Anatomy Academy’ using the Organization Search Module. Enroll yourself in the Anatomy Academy by clicking enroll in the drop box by the organization ID. 2 3 MICROSCOPE CARE AND USE Know the names of the parts of the microscope. Be able to calculate the total magnification based on the lens used. USE OF MICROSCOPES 1. CARE OF MICROSCOPES Before plugging in the microscope, make sure the light is turned off. Always carry microscopes upright with one hand on neck and 2. Plug in the microscope and turn the light source all the way up. other hand under base. 3. Start with the scanning power (4X) objective lens. • Only use lens cleaner paper to clean lenses and slides. 4. Put a slide on the stage and secure it with the holder. Do NOT • Before putting away the microscope, • • Remember to remove the slide. • Reset the lens to scanning power. • Turn off the light. • Unplug the microscope by pulling on the plug, not the push the slide UNDER the holder. 5. controls next to the right side of the stage. 6. Always place the dust cover back on the microscope. • Put the microscope in the cabinet with the ocular lens 7. Adjust the light level using the light source dial, the iris diaphragm, and raising and lowering the condenser lens. 8. going in first. • Focus using the knobs on the side of the base. Start with course focus and then fine focus. cord. • Center the slide under the light using the mechanical stage Higher magnification requires more light which increases resolution. Recenter the slide on the point of interest. Put the power cord away in one of the top drawers next 9. to your seat. 10. Increase magnification to low power (10X). 11. Focus and recenter. MAGNIFICATION 12. Increase magnification to high power (40X or 45X). Under high Total magnification is the magnification of the ocular lens (10X) power, use the fine focus knob only, course focus adjustment can multiplied by the objective lens in use. At low power, total magnification is break the slide. 100X (10X x 10X). 4 5 6 CELL STRUCTURE MODULE BE ABLE TO IDENTIFY THE MAJOR STRUCTURES AND ORGANELLES OF THE CELL Plasma/cell membrane- outer phospholipid bilayer membrane of the cell Rough endoplasmic reticulum- located near nucleus, contains ribosomes Cilia- hair like processes Smooth endoplasmic reticulum- located further from nucleus, does NOT contain ribosomes Flagella- tail-like process Golgi apparatus- the “Shipping and Distribution Center” of the cell (UPS Warehouse) Cytoplasm- all cellular components inside the membrane, but outside the nucleus Lysosomes- vesicles with digestive enzymes Nucleus Mitochondria- small bean shaped organelles that produce ATP Nuclear envelope (membrane) Nucleolus Nucleoplasm Centrioles- rod-shaped bodies Spindle fibers- formed by centrioles at beginning of mitosis Ribosomes- small organelles, function in protein synthesis 7 mitochondrion Plasma membrane Golgi apparatus vacoule Smooth endoplasmic reticulum centriole cytoplasm nucleolus Rough endoplasmic reticulum Nuclear membrane ribosome lysosome 8 9 chromatin, which are long strands like a bowl of spaghetti. When the DNA is replicated, copies are kept together by structures called centromeres. CELL DIVISION INTRODUCTION o Mitotic phase – cell is divided into two identical daughter cells in 2 steps, Mitosis and Cytokinesis. All living things are made up of one or more cells. Humans begin life as a single cell (fertilized egg) and grow to be made of trillions of cells. Two adult humans can then produce a new human. This is called the Human Life cycle. In order to grow larger and repair damage, an organism must increase the number of its cells. To produce another human (reproduce), humans must make a reproductive cell (sperm or egg) that can combine with another human’s reproductive cell. Every human cell contains the entire instruction manual (46 strands of DNA) necessary to make a fully grown human. o Mitosis – cell’s nucleus and DNA is divided into two identical nuclei. This occurs in 4 phases: Prophase, Metaphase, Anaphase and Telophase. o Prophase Chromatin condenses into chromosomes which can now be seen with a microscope. Each chromosome consists of the 2 identical copies of a DNA strand, called sister chromatids, held together at the centromere. The nuclear membrane begins to disintegrate. Spindle fibers begin to form from the centrosomes which are at either end of the cell. The spindle fibers grow and some attach to kinetochores (attachment points) on each sister chromatid. THE CELL CYCLE Cells, like humans, increase in number by going through a life cycle called the Cell Cycle. The cell cycle consists of 2 stages: o Interphase – cell grows and performs normal functions. If a cell is not going to divide it stays in G1 of Interphase. Otherwise, it continues to S phase where the DNA is replicated (copied). The DNA in the nucleus is in a form called 10 o o Telophase Metaphase The nuclear membrane disappears. The nuclear membrane reappears. Spindle fibers continue to grow pushing the cell ends apart and pushing the chromosomes to an area in the middle of the cell called the metaphase plate. The chromosomes uncoil to return to the chromatin form. The mitotic spindle fibers are disassembled. Normal nuclear (DNA) activity resumes. The kinetochore of each sister chromatid faces the opposite pole (centromere) of the cell. o Anaphase o Cytokinesis usually occurs while telophase is in progress. Spindle fibers shorten pulling on the kinetochore of each sister chromatid and pulling the centromere apart. Each sister chromatid is now considered its own chromosome. The chromosomes are pulled to opposite poles of the cell. Because identical sister chromatids were split, each cell contains an identical and complete copy of the DNA. Cytokinesis - cell’s cytoplasm and organelles are divided in two. o Cytokinesis begins with a cleavage furrow in the cell membrane that pinches the two cells apart. o The resulting two daughter cells are identical clones of the original cell, each with a nucleus and the full set of 46 chromosomes. 11 There are three different types of cells regarding the cell cycle. Permanent – cells that do not go through cell division once the tissue is mature. Stable - cells that only go through cell division to replace damaged cells Labile - cells that are constantly going through cell division. Because these cells have only half the normal DNA they are called haploid. Normal human cells with 46 chromosomes are called diploid. The creation of gametes is called meiosis. The joining of two gametes is called fertilization. Fertilization and meiosis offset each other to maintain chromosome number from generation to generation. Meiosis o Reduces the chromosomes number from diploid to haploid. o Meiosis, like mitosis, is preceded by chromosome replication. MEIOSIS o Meiosis involves 2 consecutive cell divisions, called meiosis I and meiosis II, resulting in 4 haploid daughter cells. The 46 chromosomes in each human cell are actually 2 sets of 23 chromosomes. One set is inherited from the father and one set from the mother. The 2 sets are not identical, but they are homologous. Homologous means that the chromosomes carry the same genes, such as for hair color, but the genes are not identical. Mom may have a black hair gene, while dad’s is blonde. o Meiosis I segregates the two chromosomes of each homologous pair packaging them into separate daughter cells. The centromeres do not divide. In order to create a new human with the correct 2 sets of 23 chromosomes, humans must make gametes. Gametes are cells like sperm and eggs (more properly called oocytes) that only have 1 set of 23 chromosomes. 12 Prophase I - In order for homologous chromosomes to be separated, they must first be paired together in a tetrad. Metaphase I – Tetrads line up on the Metaphase plate. WORD PARTS Anaphase I – Tetrads are pulled to opposite poles of the cell. Sister chromatids are still connected at centromeres. Telophase I and cytokinesis – The cell is pinched into two cells. The nuclear membrane does not reform before Meiosis II. o Meiosis II separates the two sister chromatids of each chromosome (centromeres divide) which is similar to mitosis. The final result of meiosis is 4 haploid gametes. Two gametes can then combine in fertilization to form a diploid cell that will become a new unique individual. 13 chroma, chromo = colored cyto = cell di = two hap = half homo = same kin = move some, soma = body tetra = four 14 15 16 THE APPENDICULAR SKELETON ANATOMIC BONE FEATURES Appendicular skeleton - all the bones of the body that make up the limbs and the shoulder and pelvic girdles • Girdles – Pectoral or shoulder – Pelvic • Upper Limbs – Arm – Forearm – Wrist – Hand • Lower Limbs – Thigh – Leg – Ankle – Foot Terms Body: main part Head: enlarged end Neck: constriction between head and body Margin or border: edge Angle: bend Ramus: branch off body Condyle: smooth rounded articular surface Facet: small flattened articular surface Projections Process: prominent projection Tubercle: small rounded bump Tuberosity: knob Trochanter: tuberosities on proximal femur Epicondyle: near or above condyle Ridges Line or linea: low ridge Crest or crista: prominent ridge Spine: very high ridge Openings Foramen: hole Canal or meatus: tunnel Fissure: cleft Sinus or labyrinth: cavity Depressions Fossa: general term for a depression Notch: depression in bone margin Fovea: little pit Groove or sulcus: deeper, narrow depression UPPER LIMB: Shoulder girdle: made up of 2 bones Clavicle • Acromial end articulates with acromion • Sternal end articulates with manubrium of sternum Scapula • Acromion process forms protective cover, attachment for clavicle, attachment for muscles • Scapular spine divides posterior surface into supraspinous fossa and infraspinous fossa 17 • • • Subscapular fossa is the anterior potion of the blade Coracoid process is an attachment for muscles Glenoid Fossa (cavity) articulates with humerus • o Arm: made up of 1 bone Humerus • Head articulates with pectoral girdle • Greater and Lesser Tubercles are attachment sites for muscles • Deltoid tuberosity • Condyles o Capitulum: rounded, articulates with radius o Trochlea: spool-shaped, articulates with ulna • Epicondyles- medial and lateral • Olecranon fossa –fits olecranon process of ulna • Coronoid fossa- fits coronoid process of ulna Radius- Lateral (thumb side) • Proximal end o Head rotates in radial notch of ulna. o Radial tuberosity: site of muscle attachment • Distal end articulates with carpals and ulna o Styloid process o Wrist: 8 bones of the wrist Carpals Carpal tunnel: on anterior surface. Enclosed by a ligament o Hand: palm and digits o Forearm: made up of 2 bones Ulna-Medial (little finger side) • o Coronoid process fits into coronoid fossa of humerus o Radial notch fits the head of the radius Distal end o Head articulates with radius and with carpals o Styloid process Proximal end o Trochlear notch fits over trochlea of humerus o Olecranon process is the point of elbow 18 Metacarpals- 5 bones of the palm • Labeled: #1-5 starting from thumb Phalanges- 3 bones making up each finger of the hand, except the thumb which only has two bones, named proximal, middle and distal phalanx and numbered #1 - #5 starting at thumb LOWER LIMB: • Pelvic Girdle: 2 coxae, sacrum and coccyx (sacrum and coccyx will be covered with axial skeleton) Coxa: each made up of 3 fused bones • • • • • Ilium- superior portion of coxa o Iliac fossa o Iliac crest o Anterior superior spine o Anterior inferior spine o Posterior superior spine o Posterior inferior spine • o Patella or kneecap: Inside tendon o Leg: made up of 2 bones Tibia- Larger of 2 and supports most of weight • • • Ischium- inferior portion of coxa o Ischial tuberosity o Ischial spine o Greater sciatic notch o Lesser sciatic notch Pubis- anterior portion o Obturator foramen (also made by part of ischium) o Pubic crest o Symphysis pubis (pubic symphysis) Acetabulum- depression made by all 3 bones, articulates with the head of the femur o Thigh: made up of 1 bone Femur• Head articulates with acetabulum • Neck Greater and lesser Trochanters: attachment for muscles that fasten lower extremity to hip Medial and lateral distal condyles: articulate with tibia Medial and lateral epicondyles: ligament attachment sites • • Tibial tuberosity: attachment site of muscle Anterior crest: shin Medial and lateral condyles articulate with condyles of femur Intercondylar eminence Medial malleolus: medial side of ankle Fibula• Head articulates with tibia not femur • Lateral malleolus: lateral wall of ankle o Ankle: Tarsals- 7 bones making up the ankle • Talus articulates with Tibia and Fibula • Calcaneus – heel bone o Foot: Metatarsals- 5 bones making up the sole of the foot • Labeled- #1-5 beginning with the big toe 19 Phalanges- 3 bones, except the big toe which only has 2 • Labeled- Proximal, Middle and Distal phalanx and #1-5 beginning with the big toe WORD PARTS coraco = bird’s beak corono = crow’s beak epi = on top of infra = inferior oid = looks like styl = pen, thin and pointed sub = beneath, under supra = superior 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 AXIAL SKELETON Axial skeleton: the bones of the body making up the skull (covered later), vertebral column, the rib (thoracic) cage, and the sternum (breastbone). Intervertebral Disks located between adjacent vertebrae Annulus fibrosus: external capsule Nucleus pulposus: internal and gelatinous cushion Disks becomes compressed with age and height decreases With age, disks are more susceptible to herniation - breakage or ballooning of the annulus fibrosus with a partial or complete release of the nucleus pulposus. May push against spinal nerves impairing function and causing pain. VERTEBRAL COLUMN: Vertebral Curvatures - Four major curvatures found in adult vertebral column Cervical: anterior Thoracic: posterior Lumbar: anterior Sacral and coccygeal: posterior At birth, column is C shaped When head is raised, cervical curve appears When sitting and walking begin, lumbar curve develops Vertebral structure Body main part of vertebra Transverse process project laterally Vertebral foramen hole for the spinal cord Spinous process project posteriorly Articular processes articulate with other vertebrae at facets Spinal nerves exit the vertebral column through intervertebral foramina. Abnormal curvatures Lordosis: Exaggeration of lumbar curvature Kyphosis: Exaggeration of thoracic curvature Scoliosis: Lateral curvature, often accompanied by kyphosis 36 Lumbar vertebrae- 5 vertebrae Large thick bodies Heavy rectangular transverse and spinous processes Vertebral regions Cervical vertebrae- 7 vertebrae Have very small bodies, tend to have bifid (split) spinous processes, and have transverse foramina Sacral vertebrae5 vertebrae fused into 1 bone, the Sacrum Alae: superior lateral parts of fused transverse processes Auricular surface: articulates with pelvic bone Median sacral crest: partially fused spinous processes Sacral foramina: intervertebral foramina Atlas Name of first cervical vertebrae Articulates with skull and allows “yes” movement No body and no spinous process Axis Name of second cervical vertebrae Dens or odontoid process extends superiorly into the vertebral foramen of the atlas Allows rotation of the atlas on the axis, the “no” movement Coccygeal vertebrae- 3-5 vertebrae fused into 1 bone the Coccyx or tailbone Thoracic vertebrae- 12 vertebrae Long, thin spinous processes directed inferiorly Long transverse processes Articular facets on transverse processes for ribs (first 10 thoracic vertebrae) Facets on body for articulation with ribs 37 RIBS WORD PARTS 12 pair of ribs protects vital organs and forms a semirigid chamber for respiration. The costal groove runs along the deep inferior surface of each rib. chondra = cartilage corn = horn True or Vertebrosternal: superior seven. Attach directly to sternum via costal cartilages. False: inferior five Vertebrochondral - superior 3 of false ribs joined by common cartilage to sternum. Floating or vertebral - most inferior 2 do not attach to sternum. costa = rib inter = between STERNUM Manubrium articulates with first rib and clavicle Body articulates with third through seventh ribs Xiphisternum or Xiphoid process HYOID Floating bone in throat. Body Greater cornu Lesser cornu 38 39 40 41 42 43 44 45 46 47 THE SKULL • • FUNCTIONS OF THE SKULL • Protect the brain • Support facial muscles • Entry for respiratory system • Entry for digestive system • Site of sense of smell, taste, sight, hearing and balance • Allow nerves and blood vessels to enter & exit brain The following is a list of the bones of the skull and their associated structures which you will be required to be able to identify for examination purposes. • CRANIAL VAULT • • • • 2 Parietal bones Frontal bone • Sagittal suture • Coronal suture • Sutures become more fused with age • Supraorbital foramen Occipital bone • Occipital condyle • Foramen magnum-opening where brain attaches to spinal cord • Lambdoid suture: between parietals and occipital 2 Temporal bones • External auditory meatus –ear canal • • 48 Mastoid Process Zygomatic process of the zygomatic arch (cheekbone) • Styloid process • Carotid canal -carotid artery enters brain through here • Jugular foramen- jugular vein exits brain through here • Mandibular fossa articulates with the mandible • Squamous suture: joins the parietal and temporal bone Sphenoid bone (greater wing) • Foramen lacerum • Foramen ovale • Optic foramen- optic nerve runs through to brain • Superior orbital fissure • Sella turcica houses the pituitary gland Ethmoid bone • Nasal conchae • Crista galli -prominent ridge in center of anterior fossa • Cribriform plate –perforated to allow nerves from nose through to brain • Perpendicular plate - part of the nasal septum along with the Vomer 2 Palatine bones THE FACE • • • • • • WORD ROOTS 2 Maxilla • Palatine processes- makes up hard palate along with palatine bones • Infraorbital foramen • Anterior Nasal Spine Mandible • Mandibular foramen • Mandibular condyle • Mental foramen • Alveolar processes • Coronoid process 2 Lacrimal Bones • Nasolacrimal canal – drains tears into nose 2 Nasal Bones 2 Zygomatic bones –front part of cheekbone Vomer –part of the nasal septum along with the Ethmoid • • • • • • • • • • • • • PARANASAL SINUSES • • Functions: – Decrease skull weight. – Resonating chambers for speech. Named for bones in which they are found. – Frontal – Maxillary – Ethmoidal – Sphenoidal 49 alveola = small sac concha = shell lacrima = tears lambda = Greek letter, λ magnum = big mast = breast oc = back of orbit = eye socket palate = roof of mouth sella = saddle squamous = fish scale suture = joint between 2 flat bones turcica = Turkish 50 51 52 53 54 55 THE MUSCULAR SYSTEM 56 MUSCLE ANATOMY MUSCLE TERMINOLOGY • Orientation: rectus, oblique • Origin or head: muscle end attached to more stationary of two bones. • Origin and insertion: sternocleidomastoid, carpi radialis • Number of heads: biceps, triceps • Insertion: muscle end attached to bone with greatest movement. • Function: adductor, masseter, flexor, extensor, supinator, pronator • Belly: largest portion of the muscle between origin and insertion. HEAD AND NECK MUSCLES • Tendons: attach muscles to bones • Agonist: muscle that, when it contracts, causes an action. • Antagonist: a muscle working in opposition to agonist. Sternocleidomastoid (Rotates to the opposite side and flexes head) • Splenius capitis (Rotates head) MUSCLES OF FACIAL EXPRESSION – Example: the biceps brachii can be used to lift weights and is the agonist, but when you move a bowling ball back to prepare to bowl, the biceps is the antagonist. • • Origin and insertion of these muscles in the superficial fascia (connective tissue beneath the skin) rather than bones. Move the skin; some act as sphincters. Synergists: muscles that work together to cause a movement. • Orbicularis oris (closes lips) NOMENCLATURE • Orbicularis oculi (closes eye) Muscles are named according to: • Risorius (laughing) • Location: pectoralis, gluteus, brachial • Zygomaticus (smiling) • Size: maximus, minimus, longus, brevis • Occipitofrontalis (Raises eyebrows and moves scalp) • Shape: deltoid, quadratus, teres, trapezius, orbicularis 57 MUSCLES OF MASTICATION • SCAPULAR MUSCLES Muscles that attach the upper limb to the body and move or stabilize the scapula and clavicle. Mastication: chewing. Involves elevation/ depression/ excursion/ protraction/ retraction of the mandible to grind the teeth together. Originate on the axial skeleton. Muscles of the cheek and tongue aid mastication by pushing the food under the teeth. • Masseter (Elevates and protracts mandible) • Temporalis (Elevates and retracts mandible) • SHOULDER MOVEMENT MUSCLES Flexes shoulder: • ABDOMINAL WALL MUSCLES • Aid in forced expiration, vomiting, defecation, urination, childbirth. Teres Major, Teres Minor, Latissimus Dorsi Abducts shoulder: • Crossing pattern of muscles adds strength to abdominal wall to support organs. Supraspinatus, Deltoid Rotates shoulder: Rectus Abdominis (Flexes vertebral column) • – Linea alba in center Subscapularis, Infraspinatus ARM MUSCLES (ROTATOR CUFF) – Tendinous intersections divided muscle into sections Primary muscles holding humerus in the Glenoid cavity. Form a cuff or cap over the proximal humerus. – Creates 6 pack abs • Pectoralis Major Extends shoulder: Flex and rotate vertebral column, decrease volume of abdominal and thoracic cavities. • Trapezius, Serratus anterior, Pectoralis major Involved in flexion, extension, abduction, adduction, rotation and circumduction. External and Internal abdominal obliques (Compresses abdomen and laterally rotate trunk) • 58 Infraspinatus, Subscapularis, Supraspinatus, Teres minor FOREARM MOVEMENT MUSCLES THIGH MOVEMENT MUSCLES Movements at the elbow Flex the hip Extends elbow: • • Extend the hip Triceps brachii Iliopsoas, Sartorius, Rectus Femoris Flexes elbow: • • Abduct the hip Biceps brachii, Brachioradialis, Brachialis • MUSCLES THAT MOVE THE HAND Gluteus Maximus Tensor fascia latae • Supinator (Supination) Adduct the hip • Pronator teres (Pronation) • Gracilis, Adductor longus Flexes wrist: Extend the hip • • Flexor Carpi Radialis, Palmaris longus, Flexor Carpi Ulnaris Flexes fingers and thumb: • Biceps femoris, Semitendinosus, Semimembranosus (Hamstrings) LEG MOVEMENTS Flex the knee Flexor digitorum, Flexor pollicis longus Extends wrist: • Sartorius • • Biceps femoris, Semitendinosus, Semimembranosus (Hamstrings) Extensor Carpi Radialis, Extensor Carpi Ulnaris Extends fingers and thumb: • Extend the knee Extensor digitorum, Extensor pollicis longus 59 • Patellar ligament and tendon • Rectus Femoris, Vastus lateralis, Vastus Medialis, Vastus intermedius (Quadriceps) FOOT MOVEMENTS WORD ROOTS biceps = 2 heads • Tibialis anterior (dorsiflexion) brevis = short • Soleus (plantar flexion) cleido = related to the clavicle • Fibularis longus (Plantar flexion and Eversion) • Extensor digitorum longus (Extension of toes) • Gastrocnemius and Calcaneal (Achilles) Tendon (Plantar flexion and flexion of leg) orbicularis = circular • Tibialis Posterior (Plantar flexion and Inversion) rectus = erect (running up and down) • Flexor digitorum longus (Flexion (curling) of toes) teres = cylindrical • Flexor hallicus longus (Flexion of big toe) delta = Greek letter delta = gastro = belly masticate = chew quadratus = 4 sided, rectangular trapezius = trapezoid shaped triceps = 3 heads 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 THE NERVOUS SYSTEM FUNCTIONS OF THE NERVOUS SYSTEM Sensory input – external and internal stimuli Integration – inputs may produce an immediate response, stored as memory, or ignored Homeostasis – regulatory and coordinating activities to maintain constant internal environment Sympathetic Nervous System Regulates “flight or fight response” Enteric Nervous System Regulates digestive functions Somatic Nervous System – voluntary muscle action o Sensory or Afferent Division - Action potentials (APs) from sensory receptors to CNS Mental activity – consciousness, thinking, memory, and emotions REFLEX ARC Control of muscles and glands Basic functional unit of nervous system. DIVISIONS OF THE NERVOUS SYSTEM Simplest portion capable of receiving a stimulus and producing a response. Central Nervous System (CNS) - Brain and spinal cord Automatic response to a stimulus that occurs without conscious thought. Homeostatic. Peripheral Nervous System (PNS) – sensory receptors and nerves Components of a Reflex Arc Stimulus → Sensory receptors → Sensory neuron → Interneuron → Motor neuron → Effector organ which responds with a reflex. o Motor or Efferent Division - APs from CNS to skeletal muscle Autonomic Nervous System – involuntary muscle and gland action Types of Reflexes Stretch reflex - Muscles contract in response to a stretching force applied to them. Parasympathetic Nervous system Regulates resting or vegetative functions 79 Withdrawal reflex - removes a limb or other body part from a painful stimulus. Some integrated within spinal cord; some within brain. Reciprocal Innervation - causes relaxation of extensor muscle when flexor muscle contracts in stretch and withdrawal reflexes. Some involve excitatory neurons yielding a response; some involve inhibitory neurons that prevent an action. Crossed Extensor Reflex - when a withdrawal reflex is initiated in one lower limb, the crossed extensor reflex causes extension of opposite lower limb to prevent falling. Higher brain centers can influence, suppress, or exaggerate reflex responses. WORD ROOTS Golgi Tendon reflex - Prevents contracting muscles from applying excessive tension to tendons by producing sudden relaxation of the muscles. Relationship of Brain and Spinal Cord Reflexes Sensory information goes to brain as well as along the reflex arc (e.g., pain.) Descending nerves from brain can exaggerate or suppress reflexes. Variety of Reflexes 80 arachna = spiders cauda = tail epi = above equine = horse homeo = same mater = mother stasis = remaining the same sub = beneath SPINAL NERVES 81 SPINAL NERVES ANATOMY STRUCTURE OF PERIPHERAL NERVES Thirty-one pairs of spinal nerves. o Nerve Axon bundles o 8 pair cervical o Myelin made of Schwann cells o 12 pair thoracic o Connective tissue o 5 pair lumbar o 5 pair sacral Endoneurium: surrounds individual neurons. Perineurium: Surrounds axon groups to form fascicles. Epineurium: surrounds the entire nerve. o 1 pair coccygeal 82 First pair exit vertebral column between skull and atlas. Last four pair exit via the sacral foramina. Others exit through intervertebral foramina. DIVISIONS OF SPINAL NERVES Spinal Nerve Division Dorsal ramus (pl. rami) Further Division or Notes Spinal nerve origins Important Nerves General Muscles Innervated Innervates dorsal trunk Cervical plexus Brachial plexus C1 – C4 C5 – T1 Ventral ramus Phrenic nerve Diaphragm Axillary nerve Deltoid muscle Radial nerve Upper limb extensor muscles Musculocutaneous nerve Arm flexor muscles Ulnar nerve Forearm flexor muscles Median nerve Forearm flexor muscles Femoral nerve Anterior thigh muscles Obturator nerve Medial thigh muscles Tibial and Lumbosacral plexus Coccygeal plexus Communicating ramus Ischiadic nerve L1 – S4 Common Fibular S4 – Co Autonomic plexuses; (T1L5) 83 Common fibular nerve Anterior/lateral leg muscles Tibial nerve Posterior thigh/leg muscles 84 85 86 87 88 89 90 91 92 93 SPINAL CORD ANATOMY GENERAL STRUCTURE OF SPINAL CORD Gray commissure - Contains axons that cross from one side of spinal cord to the other Extends from foramen magnum to second lumbar vertebra (included in gray matter) Conus medullaris – conelike region of spinal cord at L2 Cauda equina – conus medullaris plus the nerve roots at inferior end of spinal cord commissure that is continuous with fourth ventricle of brainstem Segments Cervical Segment Thoracic Segment Lumbar Segment Sacral Segment Central canal - Canal in center of gray White commissure - Contains axons that cross from one side of spinal cord to the other (included in white matter) Spinal nerve Contains sensory axons, Dorsal root ganglion (DRG) = cell bodies of Enlargements of spinal cord unipolar sensory (afferent) neurons Cervical Inferior cervical region Axons enter and leave that supply upper limb Lumbar Inferior thoracic and superior lumbar regions Axons enter and leave that supply lower limb Ventral root - Formed from 6-8 rootlets, Contains motor (efferent) axons Cross Section of Spinal Cord Dorsal root - Formed from 6-8 rootlets, Anterior median fissure and Posterior median sulcus - Deep clefts that partially divides spinal cord into right and left halves 94 COVERINGS OF SPINAL CORD (MENINGES) (Superficial Deep) Periosteum of vertebral canal Epidural space Subdural space – contains small amount serous fluid Arachnoid mater – thin and wispy like spider webs Subarachnoid space o Contains cerebrospinal fluid (CSF) o Contains blood vessels, loose connective tissue (CT), and fat Pia mater o Tightly bound to spinal cord o Site of Epidural anesthesia injected during o Filum terminale childbirth Dura mater Extension of pia mater beyond spinal cord o Outermost covering o Continous with epineurium of spinal nerves Anchors spinal cord inferiorly to coccyx 95 96 97 98 BRAIN ANATOMY Part of CNS contained in cranial cavity. Control center for many of body’s functions. Much like a complex computer but much more. Parts of the brain: o Cerebellar peduncles connects cerebellum to brainstem o Brainstem: connects spinal cord to brain; integration of reflexes necessary for survival. Hypothalamus- important in regulation of hormones o Cerebellum: involved in control of locomotion, balance, posture. Mammillary bodies: bulges on dorsal (post.) surface. o Cerebrum: conscious thought, control. Olfactory Bulb: site of the detection of smell Optic Chiasm: crossing of the 2 optic nerves Corpus Callosum – connects the left and right hemispheres Pineal body (gland) – secretes hormones Corpora quadrigemina – 4 bulges on the posterior brainstem BRAINSTEM AND MIDBRAIN Medulla Oblongata o Most inferior part, Continuous with spinal cord; has both ascending and descending nerve tracts. o Regulates: heart rate, blood vessel diameter, respiration, swallowing, vomiting, hiccupping, coughing, and sneezing. Superior: to midbrain, Middle: to pons, Inferior: to medulla oblongata o Cerebral peduncle connects brainstem to cerebrum o Midbrain: thalamus and hypothalamus. Peduncles: fiber tracts that communicate between parts of brain o Superior Colliculi – visual reflex area Pons o Inferior Colliculi – auditory reflex area o Superior to the medulla oblongata, contains Nerve tracts: ascending and descending 99 o Sulci are depressions CEREBELLUM • Attached to brainstem posterior to Pons. • Cortex folded in ridges called folia. • Arbor vitae - White matter resembling a tree. • Medulla: center • Nuclei: gray matter within the medulla made of cell bodies of neurons (like ganglion inside CNS) MENINGES • Connective tissue membranes CEREBRUM o Dura mater: most superficial layer • Largest portion of brain • Composed of right and left hemispheres each of which has the following lobes: o Arachnoid mater: middle layer o Pia mater: bound tightly to brain • o Frontal, Parietal, Occipital, Temporal • Spaces o Subdural: filled with serous fluid Sulci and Fissures o Subarachnoid: filled with CSF o Longitudinal fissure: separates the two hemispheres DURA MATER o Lateral fissure: separates temporal lobe from frontal and parietal lobes • • Superficial, tightly bound to internal periosteum except: o Central sulcus: separates frontal and parietal lobes o Falx cerebri in longitudinal fissure between the two cerebral hemispheres o Transverse fissure: separates the cerebrum from the cerebellum o Tentorium cerebelli between cerebellum and cerebrum o Falx cerebelli between the two cerebellar hemispheres. Cortex: outer surface o Gyri are folds o Venous sinuses form at the bases of the three folds. 100 VENTRICLES ARACHNOID MATER; SUBDURAL SPACE • Filled with CFS • Arachnoid Mater : a thin, wispy layer • • Subdural space: between dura and arachnoid; only a small amount of serous fluid within Lateral ventricles: within cerebral hemispheres; separated by septa pellucidum • Third ventricle: within diencephalon • Interventricular foramina join lateral ventricles with third • Fourth ventricle: associated with pons and medulla oblongata. Connected to third ventricle by the cerebral aqueduct, continuous with the central canal of the spinal cord, and connected to the subarachnoid space by the lateral and medial apertures PIA MATER AND SUBARACHNOID SPACE • Pia mater: thin, delicate C.T. membrane closely adhered to brain; follows external contours. • Subarachnoid space: contains web-like strands of arachnoid, blood vessels, and cerebrospinal fluid. WORD ROOTS CEREBROSPINAL FLUID (CSF) • Similar to serous fluid, but without most proteins. • Bathes brain and spinal cord. • Protective cushion around CNS. • Choroid plexuses produce CSF which fills ventricles and other parts of brain and spinal cord. – Composed of ependymal cells, their support tissue, and associated blood vessels. 101 • arbor = tree • chiasma = cross • corpus = body • folia = leaves • hypo = under, below • medulla = middle • olfactory = smell • vitae = life 102 103 104 105 106 107 108 109 EYE ANATOMY ACCESSORY STRUCTURES OF THE EYE LAYERS OF THE EYE Eyebrows: provide shade; inhibit sweat from entering eye Three coats or tunics (superficial to deep): Eyelids: protect the eyes from foreign objects Fibrous: sclera (whites) and cornea (transparent) Eyelashes: double/triple row of hairs. Vascular: choroid, ciliary body, iris Lacrimal Apparatus Nervous: retina Lacrimal gland: produces tears to moisten, lubricate, wash. Tears pass through ducts and then over eye. THE LENS Lacrimal canaliculi: collect excess tears through openings called puncta. Held by suspensory ligaments attached to ciliary muscles. Lacrimal sac leads to Nasolacrimal duct: opens into nasal cavity Changes shape as ciliary muscles contract and relax. Surrounded by a highly elastic, transparent capsule. Transparent, biconvex. EXTRINSIC EYE MUSCLES THE IRIS Lateral rectus The size of the pupil is controlled by the Iris which is made of two muscles. Medial rectus Superior rectus Dilator pupillae which dilates the pupil Inferior rectus Sphincter pupillae which constricts the pupil Inferior oblique Superior oblique 110 FOCUSING STRUCTURE AND FUNCTION OF THE RETINA Refraction: bending of light. Pigmented retina: single layer of cells; filled with melanin. Convergence: light striking a convex surface converges. With choroid, enhances visual acuity by isolating individual photoreceptors, reducing light scattering. Focal point: point where light rays converge and cross. Focusing: causing light to converge and focus on retina. Sensory retina: three layers of neurons: photoreceptor, bipolar, and ganglionic. Lens changes shape causing adjustment of focal point in front of the retina. Nervous signals exit the eye via the Optic nerve. VASCULAR TUNIC Ciliary body: OPTHALMOSCOPIC VIEW OF RETINA Ciliary muscles: control lens shape. Macula lutea: dark spot at center of retina. Ciliary processes: attached to suspensory ligaments of lens. Fovea centralis: center of macula lutea. photoreceptor cells tightly packed. Choroid: associated with sclera. Very thin, pigmented. Optic disc: yellow area to the side. Creates a blind spot because there are no photoreceptors here. Area through which blood vessels enter eye, where optic nerve exits from eye. Iris: colored part of the eye. Controls light entering the pupil. NERVOUS TUNIC Two layers Pigmented retina: outer, pigmented layer next to choroid. Pigment of this layer and choroid help to separate sensory cells and reduce light scattering Sensory retina: inner layer of rod and cone cells sensitive to light. 111 COMPARTMENTS OF THE EYE WORD ROOTS Anterior compartment: anterior to lens • aqueous = watery Filled with aqueous humor. • canaliculi = little canal Helps maintain intraocular pressure; supplies nutrients to structures bathed by it; contributes to refraction of light. • convex = rounded • humor = body fluid Anterior chamber: between cornea and iris • lacrima = tears Posterior chamber: between iris and lens • pigment = coloring • vascular = with blood vessels • vitreous = glassy Posterior compartment: posterior to lens. Filled with jelly-like vitreous humor. Helps maintain intraocular pressure, holds lens and retina in place, refracts light. Glaucoma: can be caused by an abnormal increase in intraocular pressure. 112 113 114 115 116 117 118 119 120 121 122 EAR ANATOMY EXTERNAL EAR INNER EAR auricle or pinna- exterior ear structure Labyrinths (chambers in the temporal bone) external auditory meatus – ear canal leading from the outside to the tympanic membrane. Cochlea: spiral labyrinth that detects sound in hearing. scala tympani –labyrinth connected to the oval window. Transmits sound waves to the cochlear duct. tympanic membrane (eardrum) – vibrates when sound wave hits it. Separates the external ear from the middle ear. cochlear duct – Contains the spiral organ with hair cells which detects the tones and volume of the sound wave. Nerve impulses are sent to the cochlear nerve. MIDDLE EAR Separated from the inner ear by the membranous oval and round windows. scala vestibule – sound wave exits the cochlear duct into this labyrinth where it is conducted to the round window. Auditory Ossicles: transmit and amplify vibrations from tympanic membrane to oval window. malleus (hammer) helicotrema - at cochlear tip. incus (anvil) Vestibule: detects static balance (equilibrium). stapes (stirrup) Semicircular canals: detects dynamic balance (equilibrium). Auditory or eustachian tube – equalizes pressure in middle ear with the outside. Oval window: is connected to the stapes and vibrates when the stapes does. Round window – dampens the sound after it has traveled through the cochlea. 123 NERVES OF THE EAR Vestibulocochlear nerve – carries nerve signals (A.P.s) from inner ear to brain. Vestibular nerve – carries nerve signals from the vestibule and semi-circular canals to the brain. Cochlear nerve – carries nerve signals from the cochlea to the brain. WORD PARTS cochlea = snail helic (helix) = spiral labyrinth = maze ossicle = bone tympanum = drum vestibule = entry space 124 125 126