University of Nebraska Medical Center
Clinical Laboratory Science Program
CLS 424 Phlebotomy
Venipuncture Collections Worksheet I
Reading Assignment in Phlebotomy Essentials, 4 th
Ed., 2008:
Chapter 5, pages 139-142
Chapter 6, pages 186-206
Chapter 7, pages 217-254
1. Proximal is defined as a.
Away from the middle b.
Closest to the middle c.
Farthest from the center d.
Nearest to the point of attachment
2. The antecubital fossa is located: a.
Anterior to and distal to the ankle b.
Anterior to and distal to the elbow c.
Posterior to and proximal to the elbow d.
Proximal to the wrist
3. The first choice vein to select for venipuncture is the: cephalic b. Basilic
4. The main difference between plasma and serum is that plasma: a.
Contains fibrinogen, serum does not b.
Contains nutrients, serum does not c.
Looks clear, serum looks cloudy d.
Looks dark yellow, serum looks pale yellow
5. It is preferable to perform STAT chemistry tests on plasma rather than serum because plasma: a.
Is more stable than serum b.
Is ready for testing sooner than serum c.
Results are more accurate d.
Tests require a smaller volume of specimen
6. During venipuncture the tourniquet should not be left on longer than: a.
30 seconds b.
1 minute c.
3 minutes d.
5 minutes
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7. A phlebotomy needle that does not have a safety feature: a.
Cannot be used for venipuncture b.
Must be used with a tube holder that has a safety feature c.
Requires immediate recapping after use d.
Should be removed from the holder before disposal
8. To what does the “gauge” of a needle relate? a.
Diameter b.
Length c.
Strength d.
Volume
9. Which needle gauge has the largest bore? a.
18 b.
20 c.
21 d.
22
10. Which of the following tubes yields a serum specimen?
11. A royal blue top tube with green color-coding on the label contains:
12. When using a butterfly to collect a protime specimen, a discard tube needs to be collected prior to filling the sodium citrate tube. This is necessary because: a. A discard tube is not necessary b. Air in the tubing will cause the first tube drawn to not fill completely c. Potential contamination from tissue thromboplastin must be eliminated d. Skin contamination could interfere with the protime test
13. When collecting any anticoagulated tube that is not liquid, e.g. EDTA, heparin or oxalates, it is recommended that the tube be inverted ______ times. a. 0
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14. For which of the following tubes is the blood to additive ratio most critical?
15. Glass or silica particles present in serum separator tubes: a. Accelerate
16. Match the additive with the appropriate tube stopper color.
__ _ __ 1. ACD a. Gold or red/black (tiger)
__ _ __ 2. EDTA
__ _ __ 3. Heparin
__
__
_
_
__ 4. No additive
__ 5. Potassium oxalate
__ _ __ 6. Silica (clot activator) b. Gray c. Green d. Lavender e. Light blue f. Red (glass tube) g. Yellow __ _ __ 7. Sodium citrate
__ _ __ 8. Sodium fluoride
__ _ __ 9. SPS
_____ 10. Thixotropic gel
17. Match the anticoagulant with its mode of action.
__ _ __ 1. EDTA
__ _ __ 2. Heparin a. Binds, chelates or precipitates calcium b. Inhibits thrombin formation
__ _ __ 3. Potassium oxalate
__ _ __ 4. Sodium citrate
18. Number the following tubes in the correct order in which they should be drawn when performing a venipuncture using vacutainer tubes.
__ _ __ 30. Gray
__ _ __ 31. Green
__ _ __ 32. Lavender
__ _ __ 33. Light blue
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