Product Datasheet
Triose Phosphate Isomerase (TPI) Activity Assay
Kit (Colorimetric) ab197001
3 Images
Overview
Product name
Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric)
Detection method
Colorimetric
Sample type
Serum, Plasma, Tissue, Adherent cells, Suspension cells
Assay type
Enzyme activity
Sensitivity
> 40 mU/ml
Species reactivity
Reacts with: Rat, Human
Predicted to work with: all Mammals
Product overview
Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) (ab197001) provides a quick and easy way for
monitoring the activity of triose phosphate isomerase (TPI) in a variety of samples. In this kit, triose phosphate
isomerase (TPI) converts dihydroxyacetone phosphate into glyceradehyde-3-phosphate, which reacts with the
enzyme mix and developer to form a colored product with strong absorbance at OD=450 nm.
The assay is simple, sensitive and high-throughput and can detect TPI activity as low as 40 mU/mL.
Notes
Triose Phosphate Isomerase (TPI, TIM, EC 5.3.1.1) is an important enzyme for glycolysis. It catalyzes the
reversible conversion of dihydroxyacetone phosphate to glyceraldehyde-3-phosphate, thus maintaining the
equilibrium of these two triose phosphates. TPI connects glycolysis to pentose phosphate pathway and lipid
metabolism. It is a stable homodimer found in almost all organisms.
In humans, TPI deficiency is a rare multisystem disorder and leads to progressive neurological dysfunction,
characterized by hemolytic anemia, cardiomyopathy and progressive neuromuscular impairment.
Tested applications
Functional Studies
Properties
Storage instructions
Store at -20°C. Please refer to protocols.
Components
Identifier
100 tests
NADH Standard
Yellow
1 vial
Positive Control
1 vial
TPI Assay Buffer
1 x 25ml
TPI Developer
1 vial
TPI Enzyme Mix
1 vial
TPI Substrate
1 vial
Pathway
Carbohydrate biosynthesis; gluconeogenesis.
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.
Involvement in disease
Triosephosphate isomerase deficiency
Sequence similarities
Belongs to the triosephosphate isomerase family.
Post-translational
modifications
The initiator methionine for isoform 2 is removed.
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Product Datasheet
Applications
Our Abpromise guarantee covers the use of ab197001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Functional Studies
Notes
Use at an assay dependent concentration.
Triose Phosphate Isomerase (TPI) Activity Assay Kit (Colorimetric) images
Relative TPI activity was calculated in lysates prepared
from rat muscle (0.5 µg), MCF-7 cells (0.8 µg), and HeLa
cells (0.84 µg).
Functional Studies - Triosephosphate Isomerase
Activity Assay Kit (Colorimetric) (ab197001)
Typical NADH Standard Curve obtained following assay
protocol.
Functional Studies - Triosephosphate Isomerase
Activity Assay Kit (Colorimetric) (ab197001)
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Product Datasheet
Kinetic measurement of Triose Phosphate Isomerase
(TPI) activity in various samples.
Functional Studies - Triosephosphate Isomerase
Activity Assay Kit (Colorimetric) (ab197001)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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