DNA as Genetic Material Genetics Bio 36404 Transmission Genetics Mendelian Genetics Classical Genetics P: Round vs Wrinkled Peas RR rr F1: All Round Rr 3/4 Round: 1/4 Wrinkled 1/4RR 2/4Rr 1/4rr F2: Cytological Genetics Chromosomes and Abnormalities Molecular Genetics • Chemistry of the Gene • What are genes made of? • How do they result in traits? • How are they replicated? • How do we manipulate them? Timeline • 1866 • 1902 Mendel Sutton - proposed genes on chromosomes • 1910 Morgan - sex-linked traits, verified genes on chromosomes • Chromosomes composed of DNA and protein 1928 Griffith Experiment Streptococcus pneumoniae inject into mouse S form - wild type smooth colonies mouse dies 1928 Griffith Experiment inject into mouse R form - rough colonies mouse lives 1928 Griffith Experiment Heat Killed S inject into mouse mouse lives 1928 Griffith Experiment inject into mouse mouse dies live S forms found in tissue Mixture of Rough and Heat-Killed S Form transformation 1944 Avery, MacLeod & McCarty S Centrifuge Heat Blender 1944 Avery, MacLeod & McCarty protease removes protein carbs proteins fats DNA RNA RNAase removes RNA carbs fats DNA RNA DNAase removes DNA carbs fats DNA carbs fats 1944 Avery, MacLeod & McCarty protease removes protein DNAase removes DNA RNAase removes RNA Conclusion? DNA causes transformation R transformation R transformation R no transformation 1952 Hershey & Chase Bacteriophages bacterial viruses Protein DNA Bacteria Viruses attach to bacteria 1952 Hershey & Chase Bacteria inject DNA or protein? new viruses grow viruses released Bacteria 1952 Hershey & Chase phage + E. coli with radioactive P phage with radioactive DNA phage + E.coli with radioactive S phage with radioactive protein 1952 Hershey & Chase radioactive phage + normal E. coli radioactive phage +normal E.coli 1952 Hershey & Chase radioactive phage + normal E. coli ? ? ? radioactive phage +normal E.coli ? ? ? Blender 1952 Hershey & Chase Conclusion: DNA enters cell radioactive DNA phage + normal E. coli ? ? ? Blender radioactive inside 1952 Hershey & Chase Conclusion: protein does not enter cell radioactive protein phage +normal E.coli radioactive outside ? ? ? Blender DNA Chemistry • DNA breaks down into 3 components • Phosphate (phosphoric acid) • Deoxyribose (sugar) • Nitrogenous bases (4) Phosphate PO4 O O- P 3- O O- O P O OP O Ribose and Deoxyribose H H 5’ H C C H O 4’ O C H H 1’ H H 3’C C O O H H C 2’ O H H O H H O C H H H C C O H H C 2’ O H Nitrogenous Base • ring contains N & C H H H H C N C N C C N pyrimidine H H C C N C C C N N H purine H Bases Pyrimidines Cytosine Thymine CH3 NH2 O O O Purines Guanine Adenine O NH2 NH2 Nucleotide P 5’ Base O sugar 1’ Nucleotide Joining P Base O 5’ sugar 1’ 3’ P 5’ Base O sugar 1’ The End