MCB 2010L
MICROBIOLOGY LABORATORY SCHEDULE
Spring 2016
TEXT: MICROBIOLOGY FUNDAMENTALS: A Clinical Approach 2nd Edition; Obenauf & Finazzo
Lab
Period
LABORATORY PROCEDURES (Exercises)
1/11 - 12
M-T
1
Lab Overview & Lab Safety Rules – Attendance Required
1 Basic Techniques: Use of the Microscope
5 The Microbial World: Protozoans
1/13 - 14
W-R
2
4 The Microbial World: Microbial Phototrophs
6 The Microbial World: Fungi
DATE
DRAW,
PHOTOGRAPH, OR
RECORD
RESULTS OF
Microscope
Protozoans
LAB REPORTS
DUE
Phototrophs
Fungi
Microscope
Protozoans
No Mon or Tue Labs – Martin Luther King Holiday on
Monday 1/18
1/18 - 19
M-T
1/20 - 21
W-R
3
1/25 - 26
4
M-T
2
7
Basic Techniques: Aseptic Techniques
The Microbial World: Motility: Hanging Drop
Hanging Drop
Phototrophs
Fungi
 Get Slide Boxes (one per team of 2 – keep in drawer)
Staining: Simple Staining & Smear Preparation
Staining: Negative Stain
Aseptic
Simple Stain
Negative Stain
Hanging Drop
8
9
Aseptic
Simple Stain
Negative Stain
Capsule Stain
Gram Stain
1/27 - 28
W-R
5
10 Staining: Capsule Stain
11 Staining: Gram Stain
Gram Stain
Capsule Stain
2/1 - 2
M-T
6
12 Staining: Acid Fast Stain
13 Staining: Endospore Stain
Acid Fast
Endospore
2/3 - 4
W-R
7
Acid Fast
Endospore
WRITTEN EXAM 1
(on lab periods 1-6)
16 Media: Eosin Methylene Blue (EMB)
2/8 - 9
M-T
8
14 Media: Blood Agar
15 Media: Mannitol Salt Agar (MSA)
17 Media: MacConkey Agar
EMB Agar
2/10 - 11
W-R
9
18 Media: Evaluation of Media
19 Growth: Standard Plate Count
20 Growth: Osmotic Pressure & Growth
MSA
Blood Agar
MacConkey
EMB Agar
2/15 - 16
M-T
10
21 Growth: Aerotolerance
25 Control: Antimicrobial Susceptibility
2/17 - 18
11
24 Control: UV Light
26 Control: Hand Washing
Eval Media
Plate Count
Osmotic Pressure
Aerotolerance
Susceptibility
12
27 Diagnostic Genetics: DNA Fingerprinting
28 Applied Genetics: Bacterial Transformation
UV Irradiation
Handwashing
MSA
Blood Agar
MacConkey
Eval Media
Plate Count
Osmotic Pressure
Aerotolerance
Susceptibility
13
 Review for Midterm Practical Exam
students may attend review sessions in other lab times
as guests
Transformation
DNA Fingerprinting
UV Irradiation
Handwashing
W-R
2/22 - 23
M-T
2/24 - 25
W-R
2/29 - 3/1
M-T
14
 MIDTERM PRACTICAL EXAM
(on lab periods 1 – 13)
 MIDTERM WRITTEN EXAM
(on lab periods 7 – 13)
MCB 2010L
MICROBIOLOGY LABORATORY SCHEDULE
Spring 2016
TEXT: MICROBIOLOGY FUNDAMENTALS: A Clinical Approach 2nd Edition; Obenauf & Finazzo
DATE
3/2 - 3
W-R
15
EXERCISE
29 Microbes & Disease: Parasitic Protozoans
31 Microbes & Disease: Vectors of Disease
RECORD
Parasitic Protists
Vectors
DUE
Transformation
DNA Fingerprinting
3/7 – 10
3/14 - 15
M-T
15
No Labs – Spring Break
3 Basic Techniques: Isolation Streak Plate
30 Microbes & Disease: Parasitic Worms
Parasitic Worms
Parasitic Protists
Vectors
3/16 - 17
W-R
16
32 Diagnostic Immunology: Immunoprecipitation
33 Diagnostic Immunology: Passive Agglutination
47 Bacterial Unknowns
Isolation Streak Plate
Passive Agglutination
Parasitic Worms
3/21 - 22
M-T
17
35 Environmental Microbiology: Soil Microbes
Immunoprecipitation
Isolation Streak Plate
Passive Agglutination
3/23 - 24
W-R
18
36 Biochemical / Hydrolytic: DNAse Test
Soil
Immunoprecipitation
3/28 - 29
M-T
19
46 “Known” bacteria for Biochemical testing
40 Biochemical Testing: Phenol Red (Sugar) Broth
Soil
DNase
3/30 - 31
W-R
20
41 Biochemical Testing: Triple Sugar Iron Agar (TSI)
Phenol Red
Soil
DNase
4/4 - 5
M-T
4/6 - 7
W-R
21
TSI Agar
Soil
Phenol Red
Phenol Red
TSI Agar
4/11 -12
M-T
23
4/13 -14
W-R
24
44 Biochemical Testing: Urease Test
45 Biochemical Testing: Nitrate Reduction
 Last day to inoculate any tests on unknowns
4/18 - 19
M-T
25
22 Oxygen Metabolism: Catalase Test
23 Oxygen Metabolism: Oxidase Test
34 Multitest systems: EnteroPluri Test
 Complete Unknown
4/20 - 21
W-R
26
4/25-26
M-T
27
 Clean up lab
 Review for Final Practical Exam
students may attend review sessions in other lab times
as guests
 FINAL PRACTICAL EXAM
(on lab periods 15 – 26)
 FINAL WRITEN EXAM
(on lab periods 22 – 26)
Written Exam 3
22
43
43
43
37
38
39
42
(on lab periods 15-20)
IMViC Testing
Biochemical Testing: SIM Medium
Biochemical Testing: MR and VP tests
Biochemical Testing: Simmon’s Citrate Agar
Biochemical / Hydrolytic: Gelatinase
Biochemical / Hydrolytic: Starch Test
Biochemical / Hydrolytic: Casease Test
Biochemical Testing: Litmus Milk
IMViC
Gelatinase
Starch
Casease
Litmus
Urease
Nitrate
Litmus
Catalase
Oxidase
EnteroPluri Test
IMViC
Gelatinase
Starch
Casease
Unknown
Litmus
Urease, Nitrate
Catalase, Oxidase
EnteroPluri