DNA & RNA Benchmark Study Guide Page1 There are 24 Finallike questions (purple, Italicized and underlined), and 6 most missed questions (circled, yellow,Italicized and underlined) 5A DNA Structure DNA & RNA Benchmark Review 3. (5A) The four bases in DNA are 1. (5A) The following molecule 2. (5A) The following is a is a/an? monomer of DNA? A. Amino Acid PhosphateGroup A. Amino Acid B. Carbohydrate B. Hydrogen bond C. DNA C. Protein D. RNA D. Nucleotide A.Adenine, thymine, guanine & cytosine B. Adenine, uracil, guanine & cytosine Deoxyribose C. Deoxyribose, uracil, thymine & Adenine CH2 C D. Ribose, uracil, thymine & Adenine O Nitrogenous Base 4. (5A) A DNA nucleotide may contain? A. Deoxyribose, uracil & a phosphate group B.Deoxyribose, guanine & a phosphate group C.Ribose, uracil & a phosphate group 6. (5A) Which of the following is 5. 5A Which molecule found in both DNA & RNA? below, represents the molecule made directly from A. phosphate group + guanine + uracil DNA during transcription B. phosphate group + guanine + cytosine and is considered the blueprint of the genetic code? A. mRNA B. tRNA C. rRNA hyperlink C. ribose + phosphate group + uracil D. deoxyribose + phosphate group + thymine DNA Strand D. nucleotide 7. (5A) Which of the following 8. (5A) Which of the following describes the structure of DNA? represent the initials for the following?tRNA,rRNA and A. Double stranded RNA molecule. mRNA B. Double helix molecule. A. Transition,ribosomal& messenger C. Double stranded RNA helix. D. Single stranded RNA molecule. 10. (5A) DNA is a polymer consisting of units called? B. Transfer, reconnaissance & messenger B. 2 C. Transfer,ribosomal & methione C. 3 D. Transfer,ribosomal & messenger D. None 1D & 5B A. Dipeptides B. Amino Acids C. Carbohydrates D. Nucleotides 9. (5A) How many different types of RNA are involved in protein synthesis? A. 1 Single Transcription & Translation StrandedRNA 11. (DNA&RNA 5b) What is produced during transcription? A. mRNA molecules C. RNA polymerase B. DNA D.proteins DNA & RNA Benchmark Study Guide Page2 TAC ATG CCG GGC AAT TTA GGC CCG CAG GTC CCG GGC ACT TGA 12. What is represented above? A. A mRNA molecule. B. A tRNA molecule. C. A mRNA and tRNA molecule. D. A DNA molecule. R N A & D N A 1D C e n tra l D o g m a F lo w o f G e n e tic In fo rm a tio n R N A P ro te in D N A#1D N A DNA # 2 (in th e N u c le u s ) RNA # 3 (in R ib o s o m e s ) P ro te in 13. Which # represents DNA Replication? A.1 B.2 C.3 D.4 16. (Cell Biology 1D) What happens during the process of translation? A. Anticodon B.the order in which amino acids are linked C. the code for splicing mRNA D. the genetic code A. Messenger RNA is made from DNA. B. The cell uses information from mRNA to produce proteins. C. tRNA is made from mRNA. D. Copies of DNA molecules are made. 18. (Cell Biology 1D) Which organelle is involved in both DNA Replication and Transcription? A. Ribosome B. Endoplasmic Reticulum C. Nucleus 19. What is the final product of this process? A. mRNA only B. tRNA only D. RNA and DNA C. No RNA A. ACTGAACG B. ACTGAATG C. ACTGAACT D. ACTGAATC Double Stranded DNA TAC CCG ATG GGC AAT GGC TTA CCG CAG GTG CCG GGC ACT TGA Two Double Stranded DNA After Replication 24. (DNA & RNA Standard 4b) what TAC CCG AAT GGC CAG CCG ACT tg ggc t ta ccg gt c ggc does athe figure below show? t g a A. Anticodon B.the order in which amino acids are linked t a c forcsplicing c g amRNA at g g D. c the c a c genetic ccg a c tcode C. the code ATG GGC TTA CCG GTG GGC TGA A. mRNA, DNA, rRNA, tRNA, protein & trait. B. DNA, mRNA, rRNA, tRNA & protein & trait. C. Transcription, translation, DNA replication, genetic trait & protein D. None of the above Double Stranded DNA 15. (Cell Biology 1D) what does the figure below show? 21. What would be the complementary strand of the following sequence during DNA replication? TGACTTAG 14. (Cell Biology 1D) Which of the following sequences of events describe the central dogma of molecular biology? D. Protein TAC CCG ATG GGC AAT GGC TTA CCG CAG C CCG GTG GGC ACT TGA Two Double Stranded DNA After Replication TAC atg CCG ggc tac ccg ATG GGC AAT GGC t ta ccg C CCG CAG g t gc g g c ACT tga aat TTA cac GTG act TGA ggc CCG ccg GGC 17.The blue lower case letters represent? A. The original DNA strands. B. The new DNA strands. C. Both the original and new DNA strands. R N A & D N A 1D C e n tra l D o g m a F lo w o f G e n e tic In fo rm a tio n D N A # 1 D N A R N A P ro te in DNA # 2 (in th e N u c le u s ) RNA # 3 (in R ib o s o m e s ) P ro te in 20. Which # represents translation? A.1 B.2 C.3 D.4 22. (Cell Biology 1D) Protein is made during translation, also known as what? A. Transcription B. DNA Polymerase C. Protein Synthesis D. RNA and DNA 23. (Cell Biology 1D) The central dogma explains which of the following? R N A & D N A 1D C e n tra l D o g m a F lo w o f G e n e tic In fo rm a tio n R N A P ro te in D N A#1D N A 26. (DNA & RNA Standard 4a) What happens during the process of translation? DNA # 2 (in th e N u c le u s ) RNA # 3 (in R ib o s o m e s ) P ro te in 25. (4a) Process #1 is known as? A.Transcription C.Translation B.translocation D.DNA Replication A. How DNA directly produces proteins B. How proteins form amino acids. C. How RNA controls the production of DNA. D. How DNA can result in genetic traits. A. Messenger RNA is made from DNA B. The cell uses information from mRNA to produce proteins. C. tRNA is made from mRNA D. Copies of DNA molecules are made DNA & RNA Benchmark Study Guide Page3 27. (4a) The tRNA anticodon GAU is complementary to this mRNA codon? 28. (4a) Which type of RNA codes for a single amino acid? 29. (4a) The mRNA codon AAU is complementary to this tRNA anticodon? A. CTU A. rRNA A. CTU B. CUA B. tRNA B. CCG C. GCG C. nRNA C. UUA D. ATA 30. (4a) What part of the tRNA codes for that specific single amino acid? A. codon D. mRNA D. ATA Translation 32. (4a) What does the figure below represent? A. Transcription B. DNA Replication B. tRNA C. anticodon 31. (4a) What is the final product of this process? C. Translation D. mRNA A. mRNA only B. tRNA only C. No RNA D. Protein D. Protein lysis 33. (4a) Which defines what a codon is? 34. (4a) During translation, the type of amino acid that is added to the growing polypeptide (protein), depends on the? 35. (4b) The codon CUC codes for what amino acid? A. Aspargine A. The strong bond between two complementary nitrogenous bases. A. codon on mRNA B. A free-floating base that attaches B. anticodon on the mRNA to an open DNA strand. C. A protein that begins transcription C. anticodon on tRNA & by breaking apart H bonds. D. The genetic code word of three bases that specify one amino acid. 36. (4a) How many nucleotides makeup a codon? codon on mRNA D. rRNA 37. (4a) How many codons would it take to specify 4 amino acids? B. Glycine C. Leucine D. Methionine 38. (4a) Which type of RNA functions as the blueprint of the genetic code? A. rRNA A. 12 A. 12 B. 9 C. 6 D. 3 B. 4 C. codons don’t specify amino acids D. 24 B. tRNA R N A & D N A 1D C e n tra l D o g m a F lo w o f G e n e tic In fo rm a tio n R N A P ro te in D N A#1D N A R N A & D N A 1D C e n tra l D o g m a F lo w o f G e n e tic In fo rm a tio n R N A P ro te in D N A#1D N A R N A & D N A 1D C e n tra l D o g m a F lo w o f G e n e tic In fo rm a tio n R N A P ro te in D N A#1D N A DNA # 2 (in th e N u c le u s ) RNA # 3 (in R ib o s o m e s ) P ro te in 39. (4a) Which # represents translation? A.1 B.2 C.3 D.4 DNA # 2 (in th e N u c le u s ) RNA # 3 (in R ib o s o m e s ) P ro te in 40. (5B) Which # represents transcription? A.1 B.2 C.3 D.4 C. nRNA D. mRNA DNA # 2 (in th e N u c le u s ) RNA # 3 (in R ib o s o m e s ) P ro te in 41. (4a) What is the product of #2? A.a DNA strand C.amino acid chain B.mRNA D.Replication DNA & RNA Benchmark Study Guide Page4 R N A & D N A 1D C e n tra l D o g m a F lo w o f G e n e tic In fo rm a tio n R N A P ro te in D N A#1D N A DNA # 2 (in th e N u c le u s ) RNA # 3 (in R ib o s o m e s ) P ro te in 42. (4a) What is the product of #3? A.a DNA strand C.amino acid chain B.mRNA D.Replication 43. What would be the complementary strand of the following sequence during DNA replication? TGACTTAG A. ACTGAACG B. ACTGAATG C. ACTGAACT TGACTTAG D. ACTGAATC ACTGAATC Double Stranded DNA TAC ATG CCG GGC AAT TTA GGC CCG CAG GTG CCG GGC ACT TGA 44. (4a) How many nucleotides would it take to specify 4 amino acids? A. 12 B. 4 C. codons don’t specify amino acids D. 24 Two Double Stranded DNA After Replication 4C & 4D Mutations & Gene Expression TAC atg CCG ggc AAT t ta GGC ccg CAG gt c CCG ggc ACT tga tac ATG ccg GGC aat TTA ggc CCG cac GTG ccg GGC act TGA 45. DNA & RNA 4C & 4D A promoter determines 46. DNA & RNA 4C & 4D This is the ultimate source of genetic variability? A. binding to the operator. A. radiation B. what genes are expressed. B. mutations C. that an expressed gene is turned off. C. hybridization D. inbreeding D. binding to the lac gene. 48. DNA & RNA 4D Skin 47. DNA & RNA 4C A cells cannot sense smell because mutation that involves a single nucleotide is called _? A. skin cell do not have the same A. point mutation B. chromsomal mutation C. translocation D. inversion 50. DNA & RNA 4C Which of the following are frameshift mutations? A. point mutation:inversion & deletion. genes as nose cells. B. the DNA is different in every cell. A. always transcribed. C. skin cells are in the wrong place. B. never expresses. D. the genes for sensing smells are turned off in skin cells. C. not regulated. 51. DNA & RNA 4D Gene regulation in the eukaryotes ________. 52. (DNA & RNA Standard 4C) What would happen to the protein if mRNA sequence mutated from CAUUUA to CAU=Histidine + CUA=Leucine CACCUA? CAC=Histidine + CUA=Leucine A. The protein will be different. B. The protein will be the same. A. usually involves operons. B. point mutation:substitution & deletion. B. is simpler than in prokaryotes. C. substitution,insertion& inversion. D. includes the action of an operator region. D. point mutation:insertion & deletion 49. DNA & RNA 4D If a specific kind of protein is not continually used by a cell, the gene for that protein is D. turned on and off at different times. C. allows for cell specialization. 54. DNA & RNA 4D Hox genes determine an animal’s__________. 55. DNA & RNA 4D Without regulatory sites, the expression of a gene would? A. pyrimidines A. basic A. Not be controlled B. purines B. size. C. other genes C. skin color. D. proteins D. eye color. 53. DNA & RNA 4D Genes contain instructions for making body plan. B. Sometimes be controlled C. Always be controlled D. Be controlled only when necessary. DNA & RNA Benchmark Study Guide Page5 5C Genetic Engineering 58. What is the advantage of Negative end using transgenic bacteria to produce human proteins? A. The human proteins produced by Positive transgenic bacteria work betterendthan those produced by humans. 56. Suppose a bacteria culture were mixed with recombinant plasmids containing a gene for resistance to penicillin. The bacterial culture was then treated with penicillin. Which of the following statements would not be true? A. Those bacteria that contain the plasmid Positive end will survive. B. The gene for antibiotic resistance is expressed in the bacteria that survive. C. The penicillin will kill the bacteria that transformed. D. Those bacteria that are successfully transformed will survive. A. Insert the human insulin gene Positive end into the plasmid. B. Extract the insulin from a bacterial culture C. Use a restriction enzyme to cut out the insulin gene from a human cell. 67. Wells 20 and 18 appear to have the same fragments that Negative end traveled the same distance, one seems darker than the other. Which is True? Positive end A. They contain the same DNA. B. They contain same fragment sizes. C. They contain different amounts of DNA. D. All of the above. B. They contain same fragment sizes. C. They contain same amounts of DNA. D. All of the above. B. Producing clones A. The DNA in her cells are identical. C. Studying human genes B. She has a funny nose. D. increasing & improving food supply C. She is genetically the same as her offspring. D. Her DNA source is a single body cell. 63. What kind of cells were used to make Dolly? 62. What does this figure show? A. body cell and egg cell. B. Egg cell only C. Body cell only D. Egg and sperm cell. A. Gel electrophoresis B. DNA sequencing C. Polymerase chain reaction D. A restriction enzyme producing DNA fragments. 65. Analyzing DNA by gel electrophoresis allows researchers to 64. One function of gel electrophoresis is to A. Separate DNA fragments. B. Cut DNA C. Recombine DNA Positive end A. They contain the same DNA. 60. Why is Dolly considered to be a clone? C. Human protein made by transgenic bacteria last longer. Negative end Negative end 59. What is the advantage of producing transgenic plants? A. Using more pesticides B. Transgenic bacteria produce human proteins in large amounts, faster, safer and less expensive. 61. To produce transgenic bacteria that make insulin, which of the following steps did scientists have to do first? 57. If two DNA samples showed an identical pattern and thickness of bands produced by gel electrophoresis, the samples contained? A. Identify similarities and differences in the genomes of different kinds of organisms. B. Compare the phenotypes of different organisms C. Cut DNA with restriction enzymes Negative end Positive end 68. During transformation, A. A prokaryote is changed into a eukaryote. B. A cell takes in DNA from outside the cell. C. Foreign DNA is inserted into a plasmid. D. A cell is mutated. 66. On an electrophoresis gel, band A is closer to the positive end of the gel than band B. Which is True? Negative end C Positive A B end A. Band B is smaller than Band A and moves faster through the gel. B. Band A is smaller than Band B and moves faster through the gel.