Chromogenic agar All images used in this file are reproduced with the permission of the Companies who produce the products illustrated. Chromogenic agar Two chromogenic substrates are combined in an agar to distinguish micro-organisms by their specific enzyme activity All images used in this file are reproduced with the permission of the Companies who produce the products illustrated. What is a chromogen • A compound that can be converted to a pigment • This compound has a chromophore that is colourless until the chromophore is modified chemically. • The chromophore is a functional group that absorbs light giving rise to colour. BioMerieux’s selective chromogenic medium for coliforms and E.coli COLI ID Chromogenic agar (by BioMerieux) distinguishing coliforms from E. coli Coliforms E. coli Merck’s selective chromogenic medium for coliforms and E.coli Coliforms and E. coli have the galactosidase enzyme. Only E. coli has the glucuronidase enzyme ChromocultTM chromogenic agar (by Merck) distinguishing coliforms from E. coli Coliforms E. coli SMID chromogenic agar (by BioMerieux) for the detection of Salmonella Oxoid’s selective chromogenic medium for Listeria spp. (blue colonies) and Listeria monocytogenes (blue colonies and opaque halos) Oxoid’s selective chromogenic medium for Enterobacter sakazakii (blue-green colonies)