Morehead State University
College of Science and Technology
DEPARTMENT OF BIOLOGY AND CHEMISTRY
BIOLOGY 317 - PRINCIPLES OF MICROBIOLOGY
COURSE SYLLABUS - Spring 2013
Time & Place:
Mode of Delivery:
Instructor:
Office:
Phone:
Hours:
E-mail:
Internet:
11:30-12:30 p.m., Mon & Wed, LA 311 (Lecture)
1:50 – 4:00 p.m. (001) or 4:10 – 6:20 (002), Mon & Wed, LA 346 (Lab)
Face to face for both lecture and lab, with Blackboard support.
Dr. Geoff Gearner
301-E Lappin Hall
783-2803
9:00 a.m. - 11:00 a.m., MW; 11:30 a.m. -12:30 p.m., TTh; or by appt.
g.gearner@moreheadstate.edu
http://moreheadstate.blackboard.com (Blackboard)
Catalog Description: BIOL 317. Principles of Microbiology. (2-4-4); I, II. Prerequisites: BIOL
171 and CHEM 112 or 201. Fundamental and applied aspects of microbiology. Prokaryotic cell
structure and morphology, diversity, metabolism, genetics and gene expression emphasized;
virology and immunology introduced. Microbiological techniques, scientific inquiry, bacterial
identifications, and project-oriented activities will be stressed in the lab.
Course Objectives: The primary objectives of BIOL 317 are to deepen and expand upon the
foundations of biological principles developed in previous courses using the prokaryotic organism
as a model, to improve vocabulary, to apply critical thinking skills, to develop laboratory skills
appropriate for the study of microbes, and to continue the student's development as a scientist.
Student Expectations: Like most Biology Majors courses, BIOL 317 is intended to actively engage
and challenge the student’s intellect. Although you will continue to develop a large amount of
scientific vocabulary, what is apparent in this course is that you will have to apply what you have
learned. Each of you must learn to think scientifically and to critically analyze material
presented. The faculty in the Department of Biological and Chemistry has set high levels of
expectation for its majors, and BIOL 317 re-enforces these performance levels. Each student will
be expected to come to class well prepared and to develop the necessary personal discipline
required for academic success.
The student learner outcomes (SLOs) for this course include the following:
By the end of the course, the student will
1. have an understanding of the importance of microbiology as both a basic and applied science;
2. possess knowledge of the cytologic, metabolic and genetic principles of microorganisms;
3. have an understanding of the impact of microbiology on the web of life, medicine, industry,
and society.
4. be competent in the laboratory skills required to culture, examine, enumerate, and identify
microbial organisms in a safe manner;
5. be able to access, navigate, and comprehend the microbiological scientific literature;
6. have the skills and ability to conduct experiments, interpret data, maintain research records,
and utilize thinking/problem solving skills in microbiology; and
7. be able effectively communicate scientific findings through the composition and presentation
of laboratory reports.
Textbooks:
Prescott, Harley, & Klein’s Microbiology, 8th ed., Willey, Sherwood & Woolverton,
2011, McGraw-Hill, St. Louis; ISBN 978-0-07-735013-0.
The Demon Under the Microscope, Thomas Hager, 2006, Three Rivers Press, Newy
York; ISBN 978-1-4000-8214-8
Lab Manual: Laboratory Exercises in Microbiology, 8th ed., Harley, 2011,
McGraw-Hill, St. Louis; ISBN 978-0-07-729281-2.
1
Lab Supplies: A marbled-cover lab notebook, the lab manual, a black fine point "Sharpie" pen,
¾-inch diameter adhesive labels, and a lab coat that can be left in the lab. Bring these items
with you to the second lab meeting and all labs thereafter.
ASSESSMENTS (Student Learning Outcomes addressed are indicated parenthetically):




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Quizzes/Miscellaneous: 150 points. Expect a daily 5-15 point quiz during the lecture
and/or lab session. Quiz questions will be drawn from material covered during recent lectures
and/or labs. Miscellaneous activities include some combination of media prep, library
assignments, in-class presentations, Internet assignments, worksheets, etc. (SLOs 1, 2, 3, 4,
5)
Laboratory Notebook: 100 points. You will be expected to maintain a laboratory notebook
documenting all of your laboratory activities. You will be expected to write an entry for each
scheduled laboratory session (unless a lecture or an exam is given), as well as follow-up visits
to the lab. (SLOs 1, 5, 6, 7)
Lab Reports: 100 points. There will be one or two formal laboratory reports (written, poster
and/or oral) over the course of the semester. (SLOs 1, 2, 5, 6, 7)
Examinations: 650 points. There will be three lecture exams scheduled during laboratory
periods throughout the semester. Lecture exam questions will range from matching, multiple
choice, fill-in-the-blank, to problem solving, short answer, and short essay. Each section of
the course builds upon previous sections therefore information covered in exam questions
may be cumulative. The Final Exam consists of two parts: part 1 will cover lecture material of
the last section of the course; part 2 will be comprehensive covering major themes of the
course (lecture and lab). The two laboratory exams will be similar in format to lecture exams
with the inclusion of a practical component to assess laboratory skills. (SLOs 1, 2, 3, 4, 6)
Grading:
Lecture
Lecture Exam I
100 pts
Lecture Exam II
100 pts
Lecture Exam III
100 pts
Quizzes/Misc.
150 pts
Final Exam
Laboratory
Lab Exam I
Lab Exam II
Lab Reports
Lab Notebook
150 pts
100
100
100
100
pts
pts
pts
pts
Total Possible Points =1000 pts
Grading Scale: 900 – 1,000 = A; 800 - 899 = B; 700 - 799 = C; 600 - 699 = D; < 600 = E
Attendance Policy: Role will be taken during each lecture and lab meeting. Each unexcused
absence from lab will result in a 10-point deduction from a student's final point total. Make-up
exams, quizzes and other missed work will be given at the discretion and convenience of the
instructor with a documented excused absence only. Refer to the University’s Excused Absences
Policy (UAR 131.02) for the procedure to make up missed work.
Academic Dishonesty: Plagiarism (submitting the work of others as your own) and cheating, as well
as the facilitation thereof, will result in zero credit for the assigned work, a report to the Dean of
Students, and possible dismissal from the course. See pgs. 10, 29 and 81 of The Eagle Student
Handbook, which is available at this link:
http://www2.moreheadstate.edu/dsl/eaglehandbook/?id=1028
Classroom/Laboratory Note: Food, gum, drink, and tobacco products are not allowed, especially in
the laboratory. The use of cell phones (including text messaging), MP3 players, and other
electronic devices is not allowed; these devices must be silenced (“airplane mode” for cell/smart
phones) and out of the instructor’s and student’s sight during all classroom and laboratory
activities. The first infraction will result in a warning to the student; subsequent
infractions will result in dismissal from that day’s activities and zero credit for that
day’s work. Students may use audio recorders to record lectures.
Drop Date: The last day to drop this class, receiving a grade of "W", is Friday, 29 March 2013.
2
Keys for Success: In order to succeed in this course, you should plan to attend all lectures, take
careful notes, and allow ample time to read and study the assigned material. You may use a tape
recorder to record lectures. You will get more out of lectures if you can skim the assigned
chapter before the lecture, then read the relevant material carefully as soon after lecture as
convenient. Recopy your notes utilizing what you recorded in class, the textbook, and
supplementary materials distributed in class or electronically. You should plan on spending at
least two hours of study time for each hour of lecture. If you begin to fall behind, make every
effort to catch up quickly; otherwise you may find yourself swamped with too much material to
assimilate before an exam. Some of you may find that attending lectures and reading the text is
all the support you need. Others will have problems with some of the material. Several forms of
support will be available to help you with difficulties you may experience:
1. Online course materials (lecture notes and PowerPoint slide handouts) will be available on
Blackboard. Review the notes prior to class, and bring them to class with you.
2. Your textbook publisher has developed a website, Microbiology Online Learning Center,
http://highered.mcgraw-hill.com/sites/0073375268/student_view0/index.html which has
plenty of interactive content that can enhance your understanding of the concepts we cover.
3. The External Links section of Blackboard is a comprehensive and continually updated list of
microbiology resources on the Internet.
4. There will be a weekly online discussion forum through Blackboard on Sunday
evenings, beginning at 9:00 p.m. This is will be an opportunity to ask the instructor
questions about recently covered material. At the Blackboard Announcements page for this
course, select “Communication” from the left side menu bar, then select “Collaboration”, then
join the “Office Hours” chat to participate. You may be prompted to download a Java
application before you can use the chat tool.
5. I am available for individual consultation regarding any aspect of the course. If you have
unanswered questions or concerns, or are in serious academic trouble, see me! You can
come by my office during regular office hours or schedule an appointment.
6. The MSU Tutoring and Learning Centers located in AY 220 and CCL 305 offer tutoring
services for BIOL 317.
Disability Statement: In compliance with the Americans with Disabilities Act, all students with a
documented disability are entitled to reasonable accommodations and services to support their
academic success and safety. Though a request for services may be made at any time, services
are best applied when they are requested at or before the start of the semester. To receive
accommodations and services the student should immediately contact the Disability Services
Coordinator in the Office of Disability Services, 204-E ADCU, 606-783-5188,
http://www.moreheadstate.edu/disability/index.aspx?id=31466.
Campus Safety Statement: Emergency response information will be discussed in class. Students
should familiarize themselves with the nearest exit routes in the event evacuation becomes
necessary. You should notify your instructor at the beginning of the semester if you have special
needs or will require assistance during an emergency evacuation. Students should familiarize
themselves with emergency response protocols at www.moreheadstate.edu/emergency.
Use of Technology: Students enrolled in BIOL 317 will be expected to use computer technology to
search the Internet for topic-related information, access course materials on Blackboard, utilize
bioinformatics tools, and to prepare lab reports/presentations. Students will utilize modern
laboratory equipment and instrumentation such as the microscope, spectrophotometer, liquid
handling equipment, centrifuges, thermocyclers, and electrophoretic equipment to conduct
laboratory exercises. The instructor will use multimedia technology to enhance classroom and
laboratory activities.
3
Date
14 January
16 January
23 January
28 January
30 January
4 February
TENTATIVE LECTURE SCHEDULE
Topic
The Relevance of Microbiology
A Brief History of Microbiology
Sterilization Methods
Survey of Microorganisms
Prokaryotic Cell Structure
Prokaryotic Cell Structure, cont.
END OF MATERIAL FOR EXAM 1
Text Reference
Chap. 1
Chap. 7
Chaps. 17 - 22
Chap. 3
Chap. 3
6
11
13
18
20
25
27
4
February
February
February
February
February
February
February
March
Microbial Nutrition
Microbial Growth
Microbial Energetics
Aerobic Cellular Respiration
Catch-Up-Day
Fermentations & Anaerobic Respirations
Carbon Dioxide Fixation; The Nitrogen Cycle
Peptidoglycan Synthesis
END OF MATERIAL FOR EXAM 2
Chap.
Chap.
Chap.
Chap.
6
11
13
25
27
1
3
8
March
March
October
March
March
April
April
April
History of Molecular Biology; DNA Structure
Bacterial DNA Replication
Bacterial Transcription
Bacterial Translation
Catch-Up-Day
Regulation of Gene Expression
Antibiotics
Horizontal Gene Transfer
END OF MATERIAL FOR EXAM 3
Chap.
Chap.
Chap.
Chap.
10
15
17
22
24
29
1
April
April
April
April
April
April
May
Bacterial Genetics
Bacterial Genomics
Viruses: General Features
Viruses: Bacteriophage T4
Viruses: Influenza Virus
Microbial Ecology
Microbe-Human Interactions
Chap. 14
Chap. 16
Chap. 25
Chap. 25
Chap. 37
Chaps. 26, 28, 29
Chaps. 30 & 31
10 May
6
7
9
10
Chap. 10
Chap. 11
Chap. 11
12
12
12
12
Chap. 13
Chap. 34
Chap. 14
FINAL EXAM, 10:15 a.m. – 12:15 p.m., LA 311
4
Date
14 January
1
16 January
2
23 January
28 January
3
4
30 January
4 February
5
6
6 February
7
11 February
---
TENTATIVE LABORATORY SCHEDULE
Lab # Topic
Exercise No.
Introduction to the Microbiology Laboratory and Lab Safety
Safety Rules
Handout (H/O); inside front cover,
Ubiquity of Microorganisms
& pgs. A31–A34 of the lab manual
DEVELOPMENT OF LABORATORY SKILLS
Microscopy - LECTURE NOTES AVAILABLE ON BLACKBOARD
(Textbook Reference - Chapter 2)
Brightfield, Darkfield & Phase Contrast Microscopy
1-4
Microscope Calibration
pgs. 5 - 6
Aseptic Techniques
13-17
Pure Cultures
16
Simple Staining
7
Gram Stain
H/O
Acid Fast Stain, Mycobacterium & Tuberculosis
9
Endospore Stain, Bacterial Spores, & Bacillus
10
Bacterial Enumeration Methods
19
LECTURE EXAM 1
8
9
10
11
12
PROJECT 1: IDENTIFICATION OF AN UNKNOWN BACTERIUM
Assign bacteria; Differential Media; Gram Stain
H/O, 16, 20, 21, & 32
Continue Unknowns – Biochemical Tests
25, 27, 32, 37, & 38
Continue Unknowns
24, 25, 31, 33, & 34
Continue Unknowns - Biochemical Tests
DNA Barcoding of Bacterial Unknowns
4 March
---
LABORATORY EXAM 1
6 March
13
PROJECT 1, Cont.
DNA Barcoding of Unknown Bacteria, cont.
11 March
---
LECTURE EXAM 2
13 March
14
PROJECT 1, Cont.
Analysis of Bacterial DNA Sequences
25 March
15
27 March
1 April
3 April
16
17
18
PROJECT 2: BACTERIAN AND ARGs IN WATERSHEDS
Collect stream water/sediment samples; inoculated mTEC, R2A
and RDM agar plates
Phenotypic analyses of environmental bacterial isolates
Phenotypic analyses of environmental bacterial isolates, cont.
Antibiotic sensitivity analysis of environmental bacterial isolates
8 April
---
LECTURE EXAM 3
10 April
15 April
19
20
PROJECT 2, cont.
PCR Amplification of Antibiotic Resistance Genes
Agarose gel electrophoresis analysis of PCR products
17 April
22 April
24 April
21
22
23
Isolation & Characterization of Staphylococci & Streptococci
Isolation & Characterization of Staphylococci & Streptococci, cont.
Isolation & Characterization of Staphylococci & Streptococci, cont.
29 April
1 May
24
---
POSTER PRESENTATIONS
LABORATORY EXAM 2
13
18
20
25
27
February
February
February
February
February
H/O
43
53-56
H/O
5
Morehead State University College of Education/NCATE Addendum
College of Science and Technology
DEPARTMENT OF BIOLOGY AND CHEMISTRY
BIOLOGY 317 - PRINCIPLES OF MICROBIOLOGY
COURSE SYLLABUS - SPRING 2013
Time & Place:
Mode of Delivery:
Instructor:
Office:
Phone:
Hours:
E-mail:
Internet:
11:30-12:30 p.m., Mon & Wed, LA 311 (Lecture)
1:50 – 4:00 p.m. (001) or 4:10 – 6:20 (002), Mon & Wed, LA 346 (Lab)
Face to face for both lecture and lab, with Blackboard support.
Dr. Geoff Gearner
301-E Lappin Hall
783-2803
9:00 a.m. - 11:00 a.m., MW; 11:30 a.m. -12:30 p.m., TTh; or by appt.
g.gearner@moreheadstate.edu
http://moreheadstate.blackboard.com (Blackboard)
“Community Engagement: A Light to and from the Mountains”
The Professional Education Unit at Morehead State University delivers rigorous, high quality
programs that prepare professionals informed by best national and international scholarship, plus
research, literature, and experiences specific to Appalachia- preparing professionals to improve the
schools, quality of life, and the communities in which they live and serve. This statement is not only the
strategic mission for the College, but it also incorporates the conceptual framework that guides all our
activities.
Conceptual Framework Outcomes (CFOs):
The Unit and the faculty within individual programs assess the degree to which its graduates:
1) Master the content knowledge, professional and the twenty – first century skills need to make an
optimal contribution to “whole” student learning in education settings.
2) Are competent in the collection and use of data to inform decision – making and to demonstrate
accountability for student learning.
3) Demonstrate professional dispositions
4) Are culturally competent and understand the regions from which they have come utilizing
knowledge and experiences to effectively “bridge the gaps” (economic, achievement, and
geographic) ensuring optimal learning for all students.
5) Engage in authentic field experiences in collaboration with committed school – based partners and
are empowered to improve the quality of education throughout this region and beyond.
The student learner outcomes (SLOs) for this BIOL 317 include the following:
By the end of the course, the student will
1. have an understanding of the importance of microbiology as both a basic and applied science;
2. possess knowledge of the cytologic, metabolic and genetic principles of microorganisms;
3. have an understanding of the impact of microbiology on the web of life, medicine, industry,
and society.
4. be competent in the laboratory skills required to culture, examine, enumerate, and identify
microbial organisms in a safe manner;
5. be able to access, navigate, and comprehend the microbiological scientific literature;
6. have the skills and ability to conduct experiments, interpret data, maintain research records, and
utilize thinking/problem solving skills in microbiology; and
6
7. be able effectively communicate scientific findings through the composition and presentation
of laboratory reports.
NCATE/ EPSB Accreditation Alignment of CFOs and SLOs:
Program:
B.S. Biology – Biology Teacher Option
Aligned with
Assessment
(point values)
BIOL 317 – Principles of Microbiology
National Science
Teacher Association –
Biology Teacher Content
Knowledge*
Description of assignment
Exam 1, 100 pts
CFO: 1
SLO: 1, 2, 3, 4, 6
Exam 2, 100 pts
CFO: 1
SLO: 1, 2, 3, 4, 6
Exam 3, 100 pts
CFO: 1
SLO: 1, 2, 3, 4, 6
Lab Exam 1, 100 pts
CFO: 1
SLO: 1, 2, 3, 4, 6
A2a-1, 2, 6, 10, 13;
A2b-15, 17, 18.
Examination that assesses students’ acquisition,
understanding and application of course content
knowledge covering lectures 1 – 6.
A2a-1, 3, 6, 8, 11;
A2b-14, 15,18.
Examination that assesses students’ acquisition,
understanding and application of course content
knowledge covering lectures 7 – 13.
A2a-1, 5, 6, 8, 12, 13;
A2b-14, 1516, 17, 18.
Examination that assesses students’ acquisition,
understanding and application of course content
knowledge covering lectures 14 – 20.
A2a-1, 2, 4, 8, 9, 10, 11;
A2b-14, 15, 17, 18, 19.
Lab Exam 2, 100 pts
CFO: 1
SLO: 1, 2, 3, 4, 6
A2a-1, 2, 3, 5, 9, 10, 12, 13
A2b-14, 15, 16, 17, 19
Quizzes, etc., 150 pts
CFO: 1
SLO: 1-5
A2a-1 – 6, 8 – 13;
A2b-14 – 19.
Lab Notebook, 100 pts
CFO: 1
SLO: 1, 5, 6, 7
A2a-1, 2, 3, 5, 8, 9, 10, 12, 13
A2b-14, 15, 16, 17, 18, 19
Lab Reports, 100 pts
CFO: 1
SLO: 1, 2, 5, 6, 7
A2a-2, 3, 4, 5, 9, 10, 11, 12, 13
A2b-14, 15, 16, 17, 18.
Final Exam, 150 pts
CFO: 1
SLO: 1-7
A2a-1 – 6, 9 - 13
A2b-14-19
Examination that assesses students’ development of
laboratory skills, as well as their acquisition,
understanding and application of course content
knowledge covering lab activities 1 – 12.
Examination that assesses students’ development of
laboratory skills, as well as their acquisition,
understanding and application of course content
knowledge covering lab activities 13 – 24.
Quizzes and miscellaneous assignments that assess
students’ acquisition, understanding and application
of course content knowledge in the lecture and lab, as
well as from out of class assignments.
This activity assesses students’ ability to maintain a
current and accurate record of their laboratory work,
as well as their ability to navigate the literature and
interpret data.
Lab reports represent formal presentations of the
results of science-based research activities. Lab
reports assess student’s ability to organize
information from the published literature and from
their own lab activities, and present that data in both
a visual and oral format.
This is a course final that covers all aspects of the
course, both lecture and lab, and assesses students’
acquisition, understanding and application of content
knowledge developed over the entire semester.
*Source: National Association of Science Teachers Secondary Science Content Analysis Form
Special Note: The EPSB Standards 1-4, while they are utilized in this course by the instructors as
they follow best practices, are not overtly instructed nor included in the assessment pieces listed
above.
Required Field Experience Hours: 0
7