Part 5: Wet Mount Slide
Prepare a wet-mount slide of your own choosing. You may
use iodine stain to enhance the detail, if desired. Draw what
you see below, under two different magnifications.
Cell Biologist: ________________________________
Date: _____________ Per: ______
Table group: ______ Microscope: ______
SCORE: ________/35
Name: ___________________
Magnification: __________
Stain used? __________
Name: ___________________
Magnification: __________
Stain used? __________
Part 6: Reflections
Describe what you have learned about cell microscopy.
__________________________________________
__________________________________________
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__________________________________________
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__________________________________________
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Self Assess: ______/6
Mr. B: ______/6
Part 1: Compound Microscope Review
Correctly label the parts. (See page 847 in your textbook.)
General Rules:
1. Always start with lowest power objective lens.
2. Never use the coarse adjustment knob with the high
power objective lens.
Calculating Magnification:
Eyepiece:
________
________
________
x
x
x
Objective Lens:
__________ =
__________ =
__________ =
Magnification:
__________
__________
__________
The last prepared slide is a cross-section of a young stem from a
pine tree.
Are these plant or animal cells? __________________
ML 1110 Pinus Stems @ 40x
Draw an arrow that points to the inner rings of the young tree, and label it “rings.” Do these cells look like they are actively growing and
dividing? ________
Draw an arrow to the outer layer of cells that are actively dividing.
Label them “actively dividing.” Can you see the nucleus in these
cells? ________
What cell part surrounds the outside of each plant cell? ____________
Self Assess: ______/4
Mr. B: ______/4
Self Assess: ______/5
Mr. B: ______/5
Part 3: Prokaryotes vs Eukaryotes
Slide set: ML 1393 Prokaryotes vs. Eukaryotes Compariset
“Prokaryotes” refers to what organisms? _________________
“Eukaryotes” refers to what organisms?
___________ ___________ ____________ ___________
ML 1010 Bacteria, Three Forms
@ 100x
ML 1010 Bacteria, Three Forms
@ 400x
The next prepared slides come from the liver of an salamander of
the genus Amphiuma These interesting animals are aquatic (live in
water), have very tiny legs, can be over 1m long, and possess neither a tongue nor eyelids!
ML 1007 Amphiuma Liver
@ 100x
ML 1007 Amphiuma Liver
@ 400x
Are these cells prokaryotic or eukaryotic? ______________
Are the cells above prokaryotic or eukaryotic?
__________________________
In the slides above, the bacteria were stained using a purple
dye called a Gram Stain (see p. 190 in your text).
⇒ Bacteria with thick cell walls appear purple and are termed
“Gram positive.”
⇒ Bacteria with thinner cell walls appear pink and are termed
“Gram negative.”
Bacteria come in 3 different shapes. Sketch and label each below. (see pp. 186-187)
Self Assess: ______/5
Mr. B: ______/5
Using complete sentences, describe the relative size of the
bacterial (prokaryotic) cells compared to the size of the liver
(eukaryotic) cells.
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________________________________________________
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Aside from size, what is the most obvious difference between a prokaryotic and a eukaryotic cell?
________________________________________________
________________________________________________
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Self Assess: ______/5
Mr. B: ______/5
Part 4: Plant vs. Animal Cells
Part 2: Crystals, Fibers, and Hair
Slide set: ML 1392 Plant vs. Animal Compariset.
Slide set: ML 1426 Beginning Microscopy Compariset.
Both plants and animals are classified as
_____________________, because their cells have nuclei
and organelles.
Are salt crystals, thread fibers, and hairs alive? _______
Are they made of cells? ________
The next slides were obtained by taking a smear sample of
the inside of someone’s mouth! These are the outer lining
cells of your mouth; when you rub your tongue against the
roof of your mouth or your cheeks, you rub off these cells.
Are these classified as animal or plant cells? _____________
ML 1004 Salt Crystals @ 40x
ML 1283 Squamous Epithelium
@ 100x
ML 1283 Squamous Epithelium
@ 400x
ML 1004 Salt Crystals @ 100x
ML 1001 Colored Threads @ 100x
ML 1001 Colored Threads @ 400x
(draw only the red thread)
ML 1003 Human, Cat, Sheep Hair
@ 100x
ML 1003 Human, Cat, Sheep Hair
@ 400x
What cell part is clearly visible inside each cell?
___________________
Describe the shape of these cells.
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Self Assess: ______/5
Mr. B: ______/5
Self Assess: ______/5
Mr. B: ______/5