Biology 220: Microbiology
Chapter 3 Exam
Note: This is not an all inclusive list of items that are on your exam! These are just the highlight
questions from each chapter that I feel are important. Do not look at these questions and expect to
see them on the exam! These are just for studying. The exam may also include questions on topics that
are not represented by the questions below!
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Choose the BEST answer.
1. Which one of the following is one of the five I’s?
a. Incubation
b. Inoculation
c. Inspection
d. Identification
e. All of the above
2. Introducing a tiny sample into a container of nutrient medium is known as
a. Incubation
b. Inoculation
c. Inspection
d. Identification
e. All of the above
3. Certain techniques are based on the concept that is an individual bacterial cell is separated from
other cells and provided adequate space on a nutrient surface, it will grow into a discrete
mound of cells called a ______________.
a. Colony
b. Group
c. Bacteriophage
d. Macrophage
4. In this technique, a small droplet of sample is spread with a tool called an inoculation loop over
the surface of the medium according to a pattern that gradually thins out the sample and
separates the cells spatially over several sections of the plate.
a. Streak Plate Method
b. Loop Dilution Technique
c. Spread Plate Technique
d. None of the above
5. In this technique, a sample is inoculated into a series of cooled but still liquid agar tubes so as to
dilute the number of cells in each successive tube in the series.
a. Streak Plate Method
b. Loop Dilution Technique
c. Spread Plate Technique
d. None of the above
6. Media can be classified by their physical state as liquid, semisolid, liquefiable solid, or
nonliquefiable solid.
a. True
b. False
7. Media can be classified by their chemical composition as either synthetic or nonsynthetic.
a. True
b. False
8. This type of culture contains only one species or type of microorganism.
a. Pure culture
b. Mixed culture
c. Contaminated culture
9. This type of culture contains both known and unknown microorganisms.
a. Pure culture
b. Mixed culture
c. Contaminated culture
10. This type of culture contains two or more known types of microorganisms.
a. Pure culture
b. Mixed culture
c. Contaminated culture
11. General purpose media are designed to grow a narrow spectrum of microbes.
a. True
b. False
12. General purpose media…
a. Includes TSA, nutrient agar and broth, and brain-heart infusion
b. Grows broad spectrum microbes
c. Nonsynthetic
d. All of the above
e. None of the above
13. Bacteria that require growth factors and complex nutrients are termed _____________.
a. Enriched
b. Nonsynthetic
c. Fastidious
d. Synthetic
e. None of the above
14. What type of medium contains complex organic substances such as blood, serum, hemoglobin,
or special growth factors that certain species must be provided in order to grow.
a. General Purpose Media
b. Enriched Media
c. Selective Media
d. Differential Media
15. A _______________ medium contains one or more agents that inhibit the growth of a certain
microbe or microbes but not another.
a. General purpose
b. Enriched
c. Selective
d. Differential
16. A _______________ medium grows several types of microorganisms but are designed to bring
out visible differences among those microorganisms.
a. General purpose
b. Enriched
c. Differential
d. Selective
17. What is the total magnification when using the high power objective?
a. 40x
b. 100x
18.
19.
20.
21.
22.
23.
24.
25.
26.
c. 400x
d. 1000x
What is the total magnification when using the low power objective?
a. 40x
b. 100x
c. 400x
d. 1000x
What is the total magnification when using the scanning objective?
a. 40x
b. 100x
c. 400x
d. 1000x
What is the total magnification when using the oil immersion objective?
a. 40x
b. 100x
c. 400x
d. 1000x
The objective forms the initial image of the specimen, called the real image.
a. True
b. False
When the real image is projected to the plane of the eyepiece, the ocular lens magnifies it to
produce a second image, the actual image.
a. True
b. False
____________ defines the capacity of an optical system to separate or distinguish two adjacent
objects or points from one another.
a. Resolving power
b. Resolution
c. Refraction
d. Both A and B
e. None of the above
Differential stains require only a single dye and an uncomplicated procedure.
a. True
b. False
Simple stains require only a single dye and an uncomplicated procedure.
a. True
b. False
Which of the following is not a differential stain?
a. Gram Stain
b. Acid-fast Stain
c. Endospore stain
d. Structural stain
e. All of the above are differential stains
Determine whether the following pictures represent:
a. scanning microscope
b. transmission microscope
27.
28.
29.