RESULTS – ENZYMES – CATALASE EXPERIMENT
Name
Part 1: Normal Catalase Activity
1. Draw a picture of a substrate an enzyme reaction and the resulting product (s). Label the active site,
substrate and products.
2. What is the equation for the enzymatic reaction in this lab?
3. What is the enzyme being studied in this lab? ______________________What is the substrate?
______________________what are the products?
Table: Catalase activity at room temperature 2 H2O2
Hypothesis: If
Title: Catalase Activity at Room Temperatur
Samples
Tube 1: Liver with initial H202
Tube 2: Liquid onto a new piece of liver
Tube 1: new H2O2 onto original liver.
then,
Reaction Rate (0-5)
4. Observe the bubbles; what gas is being released?
Has it gotten warmer or colder?
_______________ Is the reaction endothermic or exothermic? _____________________
5. What liquid is in the test tube following the reaction? ______________________ What happen when you
added more liver to this liquid? _______________________Why? (RSQ)
6.
Are enzymes reusable? How do you know?(RSQ)
Part 2: The Effect of Temperature on Catalase
1. Hypothesis: If temperature is related to enzyme activity, then
Experimental Design:
Independent Variable:
Experimental Group
Dependent variable
Control Group
Chart 2 – Effect of Temperature on Rate of Enzyme Activity
Temperature
100o C (boiled liver)
0o C
Room Temp. = ________oC
37o C
Rate of Enzyme Activity (0 – 5)
2. Graph: Remember TAILS
Title:
3. Based on the data above and the class discussion, what is the optimal temperature for mammalian enzymes.
(4 to 5 sentences- must compare and contrast USING DATA (this means numbers from results) to
support your answer)
4. Ectothermic organism’s body temperatures vary with the temperature of their surroundings. Discuss the
effect this variation might have on the functioning of enzymes in these organisms.
Part 3: The effect of pH on Catalase
1. Write a hypothesis for the effect of pH on catalase/enzymes.
If pH is related to enzyme activity, then
2. Experimental Design
Independent variable:
Experimental Group:
Data Table: The effect of pH on Catalase
pH of Sample
4
7
10
Dependent variable
Control Group:
Rate of Enzyme Activity (0 – 5)
3. Graph: Remember TAILS
Title:
4. Based on the data above and the class discussion, what is the optimal pH for mammalian enzymes. (4 to 5
sentences- must compare and contrast USING DATA to support your answer)
Note: Under certain conditions, enzymes are denatured. An enzyme is denatured when the protein molecule
loses its proper shape (disrupts the active site in the process) and cannot function. Some factors that can
denature an enzyme are high temperatures, extremes of pH, heavy metals, and alcohol.
1.
Which conditions denatured the enzyme? How do you know? Use data to support your answer.
2. Can frozen liver be reused? Cooked liver? Explain using evidence from Mrs. Thennis.