RESULTS – ENZYMES – CATALASE EXPERIMENT
Name
Part 1: Normal Catalase Activity
1. Draw a picture of a substrate an enzyme reaction and the resulting product (s). (Hint- pacman clip)
2. In the reaction that you will be studying in this lab, what is the enzyme? ______________________What
is the substrate? ______________________ What are the products? ___________________
Table: Catalase activity at room temperature
Samples
Tube 1: Liver with initial H202
Tube 1: Liquid onto a new piece of liver
Tube 2: liver into H202
Reaction Rate (0-5)
3. Observe the bubbles; what gas is being released?
Has it gotten warmer or colder?
_______________ Is the reaction endothermic or exothermic? _____________________
4. What liquid is in the test tube following the reaction?
.
5. What is the liquid composed of? ______________________
What happen when you added more liver to this liquid? _______________________Why?
6.
Are enzymes reusable? How do you know?
Part 2: The Effect of Temperature on Catalase
1. Hypothesis:
2. Experimental Design:
Independent Variable:
Experimental Group
Dependent variable
Control Group
Chart 2 – Effect of Temperature on Rate of Enzyme Activity
Temperature
0o C
Room Temp. = ________oC
37o C
100o C
Rate of Enzyme Activity (0 – 5)
3. Graph: Remember TAILS
Title:
4. Based on the data above and the class discussion, what is the optimal temperature for mammalian enzymes.
(4 to 5 sentences- must compare and contrast USING DATA to support your answer)
5. Ectothermic organism’s body temperatures vary with the temperature of their surroundings. Discuss the
effect this variation might have on the functioning of enzymes in these organisms.
Part 3: The effect of pH on Catalase
1. Write a hypothesis for the effect of pH on catalase/enzymes.
2. Experimental Design
Independent variable:
Experimental Group:
Data Table: The effect of pH on Catalase
pH of Sample
4
7
10
Dependent variable
Control Group:
Rate of Enzyme Activity (0 – 5)
3. Graph: Remember TAILS
Title:
4. Based on the data above and the class discussion, what is the optimal pH for mammalian enzymes. (4 to
5 sentences- must compare and contrast USING DATA to support your answer)
Note: Under certain conditions, enzymes are denatured. An enzyme is denatured when the protein molecule
loses its proper shape and cannot function. Some factors that can denature an enzyme are high temperatures,
extremes of pH, heavy metals, and alcohol.
1.
Which conditions denatured the enzyme? How do you know? Use data to support your answer.