Ch. 19 (Part I): Bacteria (Monera) Monera everywhere! How many “angels” can fit on the head of a pin! Discuss: What are Monera? Monera Structure = bacteria (singular: bacterium) Characteristics of Monera: -Lack membrane-bound nucleus (prokaryotes) -Ribosomes are slightly different -Smaller than almost all other organisms -Large, single molecule of DNA (w/ extra circular pieces = plasmids) -Other possible structures: ** capsule: ** flagella: whip like tail for locomotion ** cilia: ** chlorophyll: optional for autotrophic cells- inside but no chloroplasts Life Cycle **Some bacteria can reproduce every 20 minutes -At this rate: 1 bacteria => 500 (3 hrs) => 1.6 x 107 (8 hours) -Rate limiting factor = Food and Waste Reproduction & Growth -Rapid reproduction! -Bacteria can reproduce either sexually or asexually -Asexual Reproduction: most common, called binary fission -Binary fission: -Sexual Reproduction: -Conjugation: -Bacteria connect using hair-like projections called pili -Bacteria may also incorporate genetic information from dead cells in a process called transformation -Live in groups called “colonies” -Unfavorable living conditions = -Endospores: -Can survive but may be inactive for up to centuries! Origin and Diversity of Moneras Classification of Moneras Two types of Bacteria (very different) 1. (Kingdom) Archaebacteria -often live in harsh environments (hot springs, underwater vents, acid) -archae = Three types: a. Methogens: b. : Hot water (60 to 250 degrees Celsius) c. Halophiles: 2. (Kingdom) Eubacteria -Eu = true (bacteria) -so many that they are hard to classify! 2 Major Phylums (of 12 total) Phylum Characteristics: one-celled , some form colonies make own food, contain chlorophyll but not chloroplasts, most are blue green in color Examples: Nostoc, Anabaena, Oscillatoria, Gleocapsa Responsible for Phylum Tiny unicellular, prokaryotic organisms, which can be found everywhere Characteristics: one-celled, most absorb food (can be saprophytes or parasites); Other ways to identify & classify Moneras a. Cell Shape 1. 2. 3. **Also: Strepto (chains) and Staphylo (clusters) **Example: b. Cell wall composition **Gram stain -Purple = -Pink = **Used to determine susceptibilities to antibiotics, toxins they produce, and how they react to disinfectants c. Nutrition 1. : need food from another source 2. Autotrophs: : use sun/CO2 (ex. cyanobacteria) -Chemotrophs: d. Respiration 1. : use oxygen 2. Anaerobic: -Obligate Anaerobe: -Facultative Anaerobe: Moneras in the Biosphere Ecological roles **Most numerous organism on earth! Decomposers: -critical in many of the life cycles Symbiosis: -other partner may be helped, harmed, or remain unaffected -example: bacteria in mouth or stomach, excess may result in tooth decay/stomach problems Diseases Caused by Monera (The Negatives) ** **Some bacteria are very harmful : poisons or toxins produced by certain bacteria, made of lipids or carbohydrates (usually gram negative) -Exotoxins: ** **Example of bacterial diseases: Lymes disease, Strep, Cholera, Tetanus, tuberculosis (TB), diphtheria, leprosy, syphilis, typhoid fever, dysentery, gonorrhea, the plague… Frontiers in Biology (The Positives) **Photosynthetic bacteria invaluable to producing earth’s atmosphere! **Bacteria critical in molecular biology (plasmids) **Used to restore natural environmental conditions = -example: **Antibiotics: life savers, must be produced all the time to stay ahead of bacteria ** **Other uses: **Your health: Are infections good for your immune system? Ch. 19 (Part II): Viruses -From what you know about viruses and biology, are viruses living? Structure of Viruses Virus: -Smaller than the smallest cell (polio = 20 nm diameter [10-9 meters]) -They do posses structural parts: Capsid: -Nucleic acid may either be DNA or RNA, depending on the virus type! -Viruses have fewer genes than basic cells (as few as 5 genes!) Envelope: -Envelopes found only on viruses that infect animal cells -Envelope has projections used for recognition and binding to animal cells Virulent viruses: Temperate viruses: Origins & History -1892, Dmitri Ivanovsky ==> first scientist to look for cause of tobacco mosaic disease -1897, Martinus Beijerinck ==> found causing agent of TMD was inside cells -He called this infectious agent a virus (Latin for poison) -1935, Wendell Stanely ==> isolated crystals of infectious agent (bacteria do not form crystals, this proved that bacteria was not the culprit!) **Where do you think the first virus originated? **One theory is that the virus was originally part of a host cell, but somehow adapted to live on its own. Does this sound like a plausible hypothesis? Viral Replication -Viruses can not replicate on their own! They require a host. Host: -Provide all the materials that viruses need to copy themselves -A cell infected with a virus has two options: 1. Lytic Cycle: -Examples: -Process: Attachment: Virus attaches to cell Penetration: Replication/Synthesis: Virus takes control of cell and makes more virus nucleic acid & protein components Assembly: Release: Cell lyses causing release of newly produced virus New viruses ready to enter other cells 2. Lysogenic Cycle: -Examples: -Latent period involved (called Latency) -When the host cell divides, the viral DNA in replicated along with its own -The cell then enters the lytic cycle Diversity -Classifying viruses difficult due to their diversity! HIV virus Corona virus Bacteriophage Ebola virus Herpes virus TMV Methods of organization: -Shapes: 1. Filoviruses ==> Ex: 2. ==> Ex. Adenovirus, geometric shaped 3. Helical viruses ==> Ex: 4. Discuss: ==> Ex: bacteriophage, polyhedral capsule & helical tail 1. Myths of the common cold (cold weather not a cause) 2. New Rx lessens severity of common cold (zinc works too) -Host: 1. Plant 2. 3. Bacteria (bacteriophage) **Note: Most viruses invade only a specific organism (always exceptions!) Example: Exception: **Specificity due to viral proteins on capsid/envelope that attach to host receptor -Function: Ex: Retrovirus (such as HIV) = RNA virus ** ** One more time: Are viruses living? Viruses: contain either DNA or RNA Cells: structure: cell wall/membrane, cytoplasm, organelles...etc... does not grow (in size) able to reproduce by mitosis/meiosis carries out metabolic activity Smaller than Viruses!?! Viroids: -No protein coat, but folding protects them from environment -Cause millions of dollars worth of plant destruction yearly Prions: -Examples: Bovine spongiform encephalopathy (Mad Cow Disease) Kuru, Creutzfeldt-Jakob disease (human), Scrapie (sheep) Viruses in the Biosphere Ecological Roles -Varying degrees of effects Small: Drastic: -Animal viruses: distemper, rabies, and pneumonia -Plant viruses: **Major problem in society: Prescribing of antibiotics to fight viruses!Why? Frontiers of biology Beneficial uses of viruses: -Vaccines: viruses used against themselves to produce vaccines Vaccination: 1. Virus either weakened or dead 2. 3. Used to eliminate smallpox, polio, and measles -Genetic engineering: **Viruses can be used to carry genes into cells (still experimental) **Virologist: -Agriculture: **No pollution **Can be used to produce more beautiful flowers (ex. -Interferon: Ch. 20: Protists -How would you react if you found yellow blobs in your yard? -What is your idea of a protist? Characteristics of Protists The structure of Protists ** **Protists can be plant-like, animal-like, decomposers, even parasitic ** **All eukaryotic organisms! Origin and Diversity of Protists **Early eukaryotes (roughly 1.5 billion years old) may be ancestors of first unicellular protists ** **Diversity reflects several significant evolutionary developments -examples: size, shape, and colors ** Protists are organized into 3 groups, based on nutritional requirements: 1. Animal-like (heterotrophs) (nearly 65,000 species!) 2. 3. Fungus-like (decomposers) Animal-like Protists - Protozoans ** Why are they not considered animals if they are heterotrophs? -Protozoans do not have specialized tissues, organs, or systems Phylums classified according to how they move: 1. sarcodinians -”psuedo” means false, “pod” means foot (ex. ameba) -contractile vacuole: -some have hard shells, extend cytoplasm through holes -examples: -Ameba (some other protists) can form cysts to survive adverse conditions (ex. amebic dysentery) 2. zooflagellates -about 2500 species -flagella used in various ways (circular or back and forth) -example: 3. ciliophorans (ciliates) -over 8000 species -cilia: -example: Paramecium 4. sporozoans -over 6000 species -life cycles include both sexual and asexual phases -parasitic forms of protists, example: Plant-like Protists - Algae Characteristics of Algae **Plant-like because they can undergo photosynthesis ** **Unlike plants, algae do not contain specialized tissues or organs **Organized into two groups: **Diverse, 7 divisions (see useful chart on p. 527) 1. Unicellular Algae -Differences in covering and means of movement help categorize -Include: a. dinoflagellates -cellulose plates cover and give unique shapes b. -snowflake shaped organisms,thousands of possible shapes -pores in walls allow transport of materials -among the most abundant organisms in the ocean * c. euglenoids (Euglenophyta) -resemble both algae and protozoans (kind of a mix of plant and animal traits) - 2. Multicellular Algae -Considered protists due to lack of true tissues and reproductive methods are more like protists than plants -Classified according to color: 1. Green Algae (Chlorophyta) -about 7000 species -can be unicellular (ex. Chlamydomonas), colonial (ex. Volvax), or multicellular (ex. Ulva) 2. Red Algae (Rhodophyta) -about 4000 species -contain accessory pigments (phycobilins) that gather other wavelengths of light & produces distinctive color - 3. Brown Algae (Phadophyta) -about 1500 species -group included largest organism, giant kelp -some have complex life cycles called alternation of generations (see p. 528) -spore-producing stage = -gamete-producing stage = Fungus-like Protists - Molds Characteristics of Molds **Most are small and live in damp, watery places **Acts as a decomposer **Organized into three groups: 1. Plasmodial slime molds -Plasmodium -single cell with many nuclei -unfavorable conditions and plasmodium creeps elsewhere or... -Fruiting body -haploid spores combine to form diploid zygote 2. Cellular slime molds -feeding stage are single cells that move and digest food -causes cells to form clump called a psuedoplasmodium -psuedoplasmodium forms fruiting bodies (produce spores) 3. Water molds -decomposers in freshwater ecosystems -some are parasitic (attach fish gills or certain land plants) - Protists in the Biosphere Ecological Roles **Other organisms rely on these abundant organisms **Protists are a major component of Plankton (microscopic organisms that float near the surface of oceans and lakes) **Form base of many food chains **Algal component of plankton called phytoplankton ** **Also helps to maintain levels of bacteria and other organisms **Human uses -Agar; foods and food processing : chocolate milk salad dressing, sausage, soup and canned meats -Diatoms : -Alginates, carageenan: dairy stabilizers (ice cream) etc. algal proteins increase nutritional value Disease Caused by Protists **Not all protists are beneficial **Trypanosoma causes **Giardia cysts that are ingested in human cause intestinal problems **Gonyaulax **Phytophthora causes a potato disease called blight **Toxoplasma often causes birth defects or even fatal to very young/prenatal. Can be carried by birds, mice, cats, etc. Frontiers in Biology **Malaria spread by Anapheles mosquitoes carrying Plasmodium protozoa Life cycle: 1. 2. In the liver, sporozoites develop into merozoites which enter red blood cells and reproduce asexually -causes red blood cells the lyse, resulting in fever 3. 4. Mosquito bites infected individual and picks up gametocytes along with blood and process continues... **Other effects of Malaria -evolution of sickle cell anemia -drug resistance, such as chloroquine and quinine (tonic)