Pharmaceutical Nanotechnology
Pharmaceutical Nanotechnology
Development of
Gold-Chitosan-coated PLGA Nanoparticles
Marius Hittinger
28.04.2011
Supervisor:
Juniorprofessor Dr. Marc Schneider
Pharmaceutical Nanotechnology
Gold Nanoparticles
Advantages:
-Easy to prepare
-Special optical properties
-Surface modifications possible
-Stable
-Biocompatible
Haiss et al. 2007
Gold Nanoparticles
Advantages:
-Easy to prepare
-Special optical properties
-Surface modifications possible
-Stable
-Biocompatible
Giljohann et al. 2010
PLGA-Chitosan Nanoparticles
Advantages:
-Easy to prepare
-Biodegradable
-Biocompatible
Nafee et al. 2007
-High efficient drug delivery vehicle
-Permeability-enhancing properties (chitosan)
http://de.wikipedia.org/wiki/Polylactid-co-Glycolid
http://de.wikipedia.org/wiki/Chitosan
Multifunctional Nanoparticles
Park et al. 2007
Multifunctional Nanoparticles
Park et al. 2007
Multifunctional Nanoparticles
Park et al. 2007
Turkevich - Method
1.! Synthesis
2.! Characterization
3.! Preparation of combined particles
4.! Characterization
Kumar et al.
Turkevich - Method (1951)
Ojea-Jiménez et al. 2010
http://mrsec.wisc.edu/Edetc/nanolab/gold/index.html
Turkevich - Method (1951)
Ojea-Jiménez et al. 2010
http://mrsec.wisc.edu/Edetc/nanolab/gold/index.html
Preparation of PLGA-Chitosan NPs
Kumar et al. 2004
Turkevich - Method
1.! Synthesis
2.! Characterization
3.! Preparation of combined particles
4.! Characterization
Kumar et al.
Preparation - Basic ideas:
-Calculate the maximum of particles which can take place on the surface
and dilute gold solution before interaction (80%,15%,0%)
-Use undiluted gold solution and separate new particles from the AuNP -solution
(100%)
Preparation - Basic ideas:
-Calculate the maximum of particles which can take place on the surface
and dilute gold solution before interaction (80%,15%,0%)
-Use undiluted gold solution and separate new particles from the AuNP -solution
(100%)
Magnetic particles
Filtration
Centrifugation
Turkevich - Method
1.! Synthesis
2.! Characterization
3.! Preparation of combined particles
4.! Characterization
Kumar et al.
Characterization
-
+
+
+
?
TEM
SEM
AFM
NanoSight
Characterization
IR
-
+
+
-
?
ZetaSizer
+
UV/VIS
CLSM
What can I expect ?
Preston et al. 2009
What can I expect ?
Preston et al. 2009
What can I expect ?
Kumar et al. 2004
Darwich et al. 2011
What could be possible ?
Multifunctional PLGA-Chitosan-Gold-Nanoparticles
What could be possible ?
Multifunctional PLGA-Chitosan-Gold-Nanoparticles
What could be possible ?
-Alginate-Chitosan-Gold-Nanoparticles
-Cellular uptake
-Microparticles
David A. Giljohann, Dwight S. Seferos, Weston L. Daniel, Matthew D. Massich, Pinal C. Patel, and Chad A. Mirkin: Gold Nanoparticles for
Biology and Medicine Angew. Chem.(49) 3280 – 3294 2010
Spectra Wolfgang Haiss, Nguyen T. K. Thanh, Jenny Aveyard, and David G. Fernig: Determination of Size and Concentration of Gold
Nanoparticles from UV-Vis Anal. Chem.(79) 4215-4221 2007
John Turkevich, Peter Cooper Stevenson and James Hillier: A study of the nucleation and growth processes in the synthesis of colloidal gold
Discuss. Faraday Soc., 1951, 11, 55-75
Isaac Ojea-Jimnez, Francisco M. Romero, Neus G. Basts and Victor Puntes: Small Gold Nanoparticles Synthesized with Sodium Citrate and Heavy Water:
Insights into the Reaction Mechanism J. Phys. Chem. C, 2010, 114 (4), pp 1800–1804
Samer Darwich1, Karine Mougin*1, Akshata Rao2, Enrico Gnecco2, Shrisudersan Jayaraman3 and Hamidou Haidara:
Manipulation of gold colloidal nanoparticles with atomic force microscopy in dynamic mode: influence of particle–substrate chemistry and morphology, and of
operating conditions Beilstein J. Nanotechnol. 2011, 2, 85–98
Noha Nafee,Sebastian Taetz, Marc Schneider, Ulrich F. Schaefer, Claus-Michael Lehr : Chitosan-coated PLGA nanoparticles for DNA/RNA delivery: effect
of the formulation parameters on complexation and transfection of antisense oligonucleotides Nanomedicine: Nanotechnology, Biology, and Medicine (3)
173–183 2007
LayerThomas C. Preston and Ruth Signorell: Growth and Optical Properties of Gold Nanoshells Prior to the Formation of a Continuous Metallic Layer
American Chemical Society VOL. 3 ▪ NO. 11 2009
Adam D. McFarland, Christy L. Haynes, Chad A. Mirkin, Richard P. Van Duyne, and Hilary A. Godwin: Color My Nanoworld Journal of Chemical Education
Vol. 81 No. 4 April 2004
Ravi Kumar*,1, U. Bakowsky, C.M. Lehr: Preparation and characterization of cationic PLGA nanospheres as DNA carriers Biomaterials 25 1771–1777 2004
Huiyul Park, Jaemoon Yang, Sungbaek Seo, Kyujung Kim, Jinsuk Suh, Donghyun Kim, Seungjoo Haam, and Kyung-Hwa Yoo : Multifunctional
Magnetic Resonance Imaging Enhancement
small 2008, 4, No. 2, 192 – 196
Nanoparticles for Photothermally Controlled Drug Delivery and
Thanks !
Pharmaceutical Nanotechnology
Thanks !
Free gold nanoparticles easily aggregate when the environment conditions change. Here, gold
nanoparticles (AuNPs) with average diameter of 3.7 nm were prepared and then modi#ed with
poly(ethylene glycol) (PEG) to improve stability. The gold nanoparticles were #rst surfacemodi#ed with 3-mercaptopropionic acid (MPA) to form a self-assembled monolayer and
subsequently conjugated with NH2-PEG-NH2 through amidation between the amine end
groups on PEG and the carboxylic acid groups on the particles.
Turkevich - Method
Kumar et al.
Goldpartikel
PLGA-Chitosankern