STUDY GUIDE
UNIT TWO – BIO 220
METABOLISM, CONTROL AND GROWTH OF MICROBES
This document is to help you with studying for the quiz. While you are allowed to use handwritten
notes on the quizzes I would not advise using this as a substitute for studying as there is a time limit on
the quizzes that will leave you pressed if you are endlessly flipping through your notes. In addition,
everything in class and the reading assignments is fair game for these quizzes, so do not neglect the
reading. I make no claim that these study guides are exhaustive, just helpful. Good luck to all of you.
READING ASSIGNMENTS
Tortora (10th Edition), Chapters 5-7
SELF-DIRECTED QUESTION
Chapter Five
1. Explain the different stages of an energy diagram describing both an enzyme catalyzed and an
uncatalyzed reaction. Be able to define the stages on a provided energy diagram.
2. What is the difference between a catalyst and an enzyme?
3. How does the primary structure of a protein play into its enzymatic properties?
4. Explain the difference between competitive and noncompetitive inhibition.
5. Contrast the energy carrying properties of ATP and NAD+.
6. Describe in detail the process of glycolysis and how one alternative pathway differs from
glycolysis.
7. Given that glycolysis generates excess energy, what is the fundamental reason behind the next
step, be it fermentation, aerobic respiration or anaerobic respiration?
8. Contrast the difference between fermentation, aerobic respiration, and anaerobic respiration.
9. Describe the complete aerobic respiration of glucose.
10. Explain the process of chemiosmosis.
11. How could knowledge of metabolism be applied to microbial identification?
Chapter Six
1. Describe the way in which different temperature ranges influence bacterial growth.
2. What are the major chemical components needed in media and each of their uses?
3. What other components, than the major chemicals, are needed to support bacterial growth?
4. What are the advantages and disadvantages to chemically defined media and complex media?
5. Compare and contrast Direct and Indirect enumeration methodologies.
6. Demonstrate an understanding of microbial growth kinetics and the mathematics used to
describe them.
Study Guide
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Chapter Seven
1. What is the difference between sterilization and commercial sterilization?
2. What will happen to exposed bacteria if a bacteriostatic reagent is removed?
3. List the major factors that influence effectiveness of antimicrobial treatments.
4. What are the two main modes of action for microbial control agents?
5. List three physical methods of microbial control, with advantages and disadvantages for each.
6. Why don't autoclaves effectively sterilized materials in tightly wrapped packages?
7. Why is ionizing radiation more effective at microbial control than nonionizing radiation?
8. Demonstrate an understanding of chemical methods of microbial control.
9. Understand the list of classes of microbes in order of how effective microbial treatments are
against them (see figure 7.11).
Study Guide
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