Name Block Date Crosses Involving Two Traits - Predict produced by two parents, when studying (genes) o Example – - and Law of : o Two genes will be separately ( Example – of one another) does not depend on eye color o Any is possible for the two traits Performing Genetic Crosses - Two trait crosses ( Crosses) 1. Determine of parents – There will be a total of for each parent (2 for each gene) Example: Seed color: G= g= Stem height: T= t= First parent: Homozygous green seed, short plant = Second parent: Heterozygous green seed, homozygous tall plant = Cross = 2. Determine Each gamete will have A maximum of Use FOIL: Example Alleles for GGtt for gene, or Ex: One T and one G produced by each parent Alleles for GgTT of alleles are possible total alleles 3. Set up Punnett Square Each set of one trait crosses When filled in, each box should include should be placed at each column or row, similar to (two for each gene) Arrange alleles in – G’s together, and T’s together Example: GGtt x GgTT 4. Determine (Genotype ratios aren’t needed) possible phenotype combinations: Ratio setup: The ratio should add up to (for the 16 boxes in Punnett Square) Example: Determine the phenotype ratio for the Punnett Square above: o P– Practice: - For each problem, determine the gametes formed by each parent, and complete the Punnett Square. Report your results by giving the phenotype ratios for the possible offspring produced by each cross. Be sure to give the genotypes of the parents if not already given. Genes: o Seed color - G = Green, g = yellow o Plant height - T = tall, t = short o Flower position - A = axial, a = terminal o Pod shape - I – inflated, i = constricted 1. Cross the parents below: Ggtt x GGTt a. What is the phenotype of the parent that is Ggtt? b. What is the phenotype of the offspring that is GGtt? 2. Cross the parents below: AaII x AaIi a. What is the phenotype of the parent that is AaIi? b. What is the phenotype of the offspring that is AAIi? 3. Cross a plant that is homozygous tall, heterozygous inflated pod, with a plant that is short, constricted pod. 4. Cross two plants that are heterozygous tall, heterozygous green seeded.