Course Syllabus
SCID 509 Separation Techniques
Academic Year 2015
Course ID and name: SCID 509
Separation Techniques
Course coordinator: Dr. Ornchuma Itsathitphaisarn
Instructors:
1. Dr. Ornchuma Itsathitphaisarn (OI)
4. Dr. Laran T. Jensen (LJ)
2. Dr. Tuangporn Suthiphongchai (TS)
5. Dr. Waraporn Komyod (WK)
3. Dr. Kittisak Yokthongwattana (KY)
Credits:
Curriculum:
Semester offering:
Prerequisite:
1 (0-2-1)
Master of Science Program in Biochemistry (required course)
Doctor of Philosophy Program in Biochemistry (required course)
Second semester
None
Course Description:
Separation of biomolecules and biochemicals based on size, shape, charge and state by using basic
techniques such as centrifugation, chromatography, electrophoresis and dialysis
Course Outline:
This course is divided into two parts. The lecture session will provide a foundation on physical, chemical
and biological principles behind commonly used methods for purifying macromolecules, including
chromatography, HPLC, centrifugation and electrophoresis. In the second half of the course, students will
apply the separation techniques to purify proteins in a virtual protein purification software as well as an
affinity-tagged protein from an actual bacterial lysate.
Course Learning Outcomes (CLOs)
Upon completion of this course, students are able to:
1. Explain physical, chemical, and/or biological principles behind techniques used to separate
macromolecules.
2. Critically evaluate, judge and discuss rationales behind purification methods for isolating
macromolecules in scientific research
3. Plan, carry out, evaluate and present a protocol for purifying a macromolecule based on the
physical, chemical and biological properties of the macromolecule.
Constructive Alignment of Course Content to CLOs and Program’s ELOs
Course Content & Activities
1. Lectures on physical, chemical and biological principles behind
chromatography, HPLC, centrifugation and electrophoresis
2. Plan and perform a virtual protein purification laboratory using a ProtLab
software
3. Give a presentation in English on the virtual protein purification result
4. Plan and perform purification of an affinity tagged protein from bacterial
lysate
5. Evaluate and write up a report on the affinity chromatography lab
6. Examination
CLOs
Program
ELOs
1
4
3
3
2
2, 4
3
3
2
1
2
4
Course Schedule:
• Lecture, virtual lab and presentation: 13:00 – 15:00 h in room B301, B Wing, Department of
Biochemistry
• Affinity purification lab and examination: 8:00-16:00h in multidisciplinary laboratory (MDL 4),
Faculty of Science
Lesson
#
1
2
3
4
5
6
Topic
General principle of separation
Column chromatography
Chromatography: HPLC, FPLC
Electrophoresis and 2D DIGE
Centrifugation
Virtual laboratory (ProtLab)
7-9
Affinity chromatography lab
10
Virtual lab presentation
11
12
13
Wet lab discussion
Q & A session (optional)
Examination
Teaching & learning
strategy
Lecture
Lecture
Lecture
Lecture
Lecture
Discussion, virtual
laboratory
Discussion, hand-on
laboratory
Seminar
*Submission of virtual
lab homework and wet
lab write-up
Discussion
Discussion
-
Assessment
Examination, feedback
Examination, feedback
Examination, feedback
Examination, feedback
Examination, feedback
Homework assignment,
Rubric
Rubric
Staff
OI
OI
KY
TS
LJ
OI, WK, TS
OI, WK, TS
Rubric
OI, WK, TS
Rubric
-
OI
OI
OI
Course Assignments
1. Homework based on the virtual protein purification software, ProtLab
2. Presentation of students’ results from the virtual protein purification software
3. Affinity chromatography laboratory write-up
Assessment Criteria
Evaluation methods
Weight (%)
Virtual lab presentation
15
Virtual lab homework
15
Wet lab participation and report
20
Examination (Lecture & lab)
50
Student’s achievement will be graded using symbols: A, B+, B, C+,C, D+, D and F based on the
distribution of students’ scores from the whole course.
Appeal Procedure
Should the students have any appeal regarding the assessments or grade, inquiry can be made to the
instructors and/or the course coordinator immediately either by direct contact, telephone or email.