ANTI-MICROBE
DIN # 02248351
Alcohol Free
Instant hand foaming sanitizer
Kills 99.99% of germs
DESCRIPTION
Antiseptic hand foaming sanitizer that kills, within seconds,
99.99% of most diseasecausing germs related to SARS, colds and flu. Helps prevent infections in minor cuts and
abrasions.
DIRECTIONS
Put a thumbnail size amount in your palm and rub hands together briskly until dry. No rinsing
necessary.
WARNING
Keep out of reach of children. Product intended for external use only. Contact with eyes should
be avoided and should this occur, the eyes should be flashed with water.
ACTIVE INGREDIENT
Benzalkonium chloride 0.3%
FABRIQUE AU / MADE IN CANADA
Questions ?
H4S 1V8
Efficacy Data: Published by NO RINSE LABORATORIES (Ohio, USA)
The efficiency of No Rinse Foaming Hand sanitizer with
0.24% Alkyl Dimethyl Benzyl Ammonium Chloride (Benzalkonium chloride)
In-Vitro Antimicrobial Test Procedures and Protocols:
1. Each test organism was grown overnight on Trypticase-soy agar slants at 35°C. Cell
suspensions were prepared by adding 10-ml sterile saline (0.9%) to each slant and gently scraping the
slant surface. Microbial densities of each cell suspension were estimated using the viable plate count
method.
2. Test product (1-ml) was aseptically added to sterile test tubes and then inoculated with a 1:10 dilution
of a cell suspension (100uL) of the test organism. At selected time intervals (0.5, 1.0 and 2.0 minutes),
aliquots (10uL) were aseptically removed and transferred to a Trypticase-soy broth recovery medium (10mL). Microbial growth was monitored by the development of turbidity in the recovery medium
TEST Results:
The active Quaternary Ammonium Chloride exhibited strong germicidal activity against a variety of gram-positive
and gram-negative, as well as the yeast Candida albicans. In most cases viable cell numbers were reduced by
greater than 99.99% after a 30 second exposure period with this product.
Initial
Exposure Time (Minutes)
Reduction
Inoculum
0.5
1.0
2.0
(percent)*
Test Microorganisms
(cfu/10uL)
Grown in TSB
Pseudomonas aeruginosa
Klebsiella pneumonia
Escheria coli
Salmonella typhimurium
Staphylococcus aureus
ATTC33591
Staphylococcus Epidermidis
Streptococcus faecalis –
ATTC522A
Streptococcus agalactae
Micrococcus luteus
Candida Albicans
Trichophytin
Mentogrophytes
(Athlete’s Foot)
Salmonella Chlorocraesuis
Aspergillus Niger
Listeria Monocytogenes
Clostridium difficile
Human Coronavirus
(Resembles SARS-like virus
family)
3.39 x 10
5
2.76 x 10
5
15.8 x 10
5
18.9 x 10
5
21.2 x 10
5
-
5
-
-
-
99.99
99.99
99.99
99.99
99.99
-
99.99
99.99
(Methicillin Resistant / MRSA)
18.3 x 10
5
9.8 x 10
(Vancomycin resistant entercococci /
VRE)
5
12.1 x 10
5
14.4 x 10
5
12.6 x 10
5
9.6 x 10
5
14.1 x 10
5
11.8 x 10
17.9 x 105
1.1 x 105
-
-
-
-
-
-
-
99.99
99.99
99.99
99.99
99.99
99.99
0 CFU/ML (30 seconds)
0 CFU/ML (15 seconds)
0 CFU/ML (15 seconds)
(*) Indicates percentage reduction in numbers of viable cells evidenced by lack of growth in Trypticase-soy broth
medium. (-) Indicates no survival of test organisms in the recover medium.
This study is performed by NO RINSSE LABORATORIES, LLC. 900 E: Franklin Street, Centerville, Ohio 45459 USA
 PROVEN TO KILL HUMAN CORONAVIRUS
(resembles the SARS-like virus family)
 EFFECTIVE IN KILLING C. DIFFICILE BACTERIA
 WILL NOT REMOVE FINGERNAIL POLISH
 NON DRYING
 NON-FLAMMABLE
 INCREASED USE COMPLIANCE
Each application requires 65% less volume than alcohol gels
NO RINSE
ALCOHOL FREE
50 ml pump
HAND
SANITIZER
Patent Pending
ANTI-MICROBE
DIN#02248351
Approved by Health Canada and Agriculture Canada
ALCOHOL-BASED
HAND SANITIZING GEL
SANITIZING INGREDIENT
%COMMON BACTERIA KILLED
IN 30 SECONDS
DRYING TIME
FLAMMABILITY RATING
HAZARDOUS MATERIAL
POSSIBLE IRRITATION TO
HANDS
AMMOUNT / APPLICATION
PRODUCT FORM
MAINTENANCE HAZARDS
70% Ethyl Alcohol
99.99
Instantly when rubbed in
4
Yes FLAMMABLE
Drying to skin from repeated use
or use during cold whether
May irritates cuts and abrasions
Removes nail polish
1.5 ml
Gel
Alcohol may harm floor finish
1.5 L wall-mounted pump
ANTI-MICROBE
NO-RINSE ALCOHOL-FREE
ANTIBACTERIAL FOAMING
HAND SANITIZER
Benzalkonium chloride
99.99
Soon after being rubbed in
0
No
Excessive use may cause
temporary dryness
0.4 ml
Foam
None
COMPARATIVE ANALYSIS BETWEEN
ALCOHOL BASED HAND SANITIZER &
BENZALKONIUM CHLORIDE FOAMING HAND SANITIZER
Woodward Laboratories compared alcohol-based gels with HandClens (Benzalkonium chloride based foaming
hand sanitizer). HandClens has been the subject of four scientific investigations. Two addressed the products
efficacy against the Federal Guidelines for antiseptic hand washes and healthcare personnel hand washes. The
results of these studies are represented by the charts below. With repeated use of alcohol-based sanitizers germkilling effectiveness (antimicrobial persistence of activity) is reduced by the drying effect of alcohol which leaves
microscopic cracks in the skin that can allow bacteria to become trapped or hidden.
HandClens is 99.99% effective against the most frequent disease and illness causing germs.
Woodward’s HandClens Kill Time Study
The following are just some of the pathogens killed within 15 seconds of exposure to HandClens.
Candida albicans
Candida keyfr
Escherichia coli
Enterococcus faecalis
Enterococcus faecium (VRE)
Klebsiella pneumonia
Microcoocus luteus
Pseudomonas aeruginosa
Proteus mirabilis
Salmonella typhimurium
Serratia marcescens
Staphylococcus aureus
Staphylococcus aureus (MRSA)
Salmonella enteritidis
Staphylococcus epidermidis
Staphylococcus haemolyticus
Staphylococcus saprophyticus
Streptococcus pyogenes
Herpes simplex virus Type 1
Human Coronavirus (related to SARS)
Trichophyton mentagrophytes
Trichophyton rubrum
Apergillis niger
Hepatitis A and B
In vitro tests performed by SCI Laboratories, Inc.; revised protocol of CFR 333.470, Woodward Laboratories, Inc.;
revised protocol of CFR 333.470, Viromed Laboratories, Inc.; revised protocol of ASTM E1052, and ATS
Laboratories, Inc.; protocol of WLI01041603.COR
ANTI-MICROBE
(DIN#02248351)
Benzalkonium chloride based hand sanitizer
BIOLOGICAL PROPERTIES
Phenol Coefficients
Phenol Coefficients of benzalkonium chloride 0.3% active were determined by
the official A.O.A.C procedure
10- Minute Killing Dilution
Organism
Dilution
of
Anti-microbe
in water to get the
10 minute killing
Concentration
of
Anti-microbe
(ml/L) to kill in
10 minutes
Phenol
Phenol
Coefficient
Brucella abortus
Escherichia coli
Klebsiella pneumoniae
Lactobacillus casei
Listeria monocytogenes
Mycobacterium amegmatis
Neisseria caiarrbalis
Pasteurella multocida
Proteus vulgaris
Pseudomonas aeruginosa PRD-10
Salmonella gallinarum
Salmonella pullorum
Salmonella typhimurium
Salmonella schottumelleri
Salmonella typhosa
Shigella sonnei
Staphylococcus aureus
Streptococcus fecalis
Streptococcus pyogenes C-203
Streptococcus viridans
FUNGI
Saccharomyces cerevisiae
1/152.6
1/101.25
1/93.75
1/393.75
1/270
1/78.75
1/64.89
1/202.89
1/45
1/52.25
1/105
1/93.75
1/75
1/225
1/168.75
1/93.75
1/168.75
1/562.5
1/93.75
1/262.5
6.55 ml/L
9.87 ml/L
10.66 ml/L
2.54 ml/L
3.70 ml/L
12.70 ml/L
15.41 ml/L
4.92 ml/L
22.22 ml/L
19.14 ml/L
9.52 ml/L
10.66 ml/L
13.33 ml/L
4.44 ml/L
5.92 ml/L
10.66 ml/L
5.92 ml/L
1.77 ml/L
10.66 ml/L
3.80 ml/L
1/110
1/70
1/90
1/100
1/100
1/65
1/70
1/110
1/70
1/70
1/80
1/90
1/70
1/95
1/90
1/80
1/60
1/70
1/80
1/90
1.387
1.446
1.042
3.937
2.700
1.211
0.927
1.844
0.642
0.746
1.312
1.042
1.071
2.368
1.875
1.172
2.812
8.028
1.172
2.916
1/187.5
5.33 ml/L
1/100
1.875
Pityrosporium ovale
1/131.25
7.61 ml/L
1/100
1.312
Microbicidal-Microbiostatic Activity
The antibacterial effectiveness of benzalkonium chloride 0.3% has been measured by an
empirical broth dilution procedure in which the highest dilutions capable of inhibiting growth to
48 hours (microbiostatic) and killing all organisms in 24 hours (microbicidal) are determined.
Organism
Brucella abortus
Penicillium luteum
Penicillium notatum
Aerobacter aerogenes
Bacillus aerus, var. mycoides
Bacillus subtilis
Brevibacterium ammonigenes
Klebsiella pneumoniae
Lactobacillus casei
Proteus vulgaris
Pseudomonas aeruginosa PRD-10
Salmonella gallinarum
Salmonella pullorum
Salmonella typhimurium
Salmonella schottumelleri
Salmonella typhosa
Salmonella choleraesuis
Shigella sonnei
Staphylococcus aureus
Trichophyton interdigitale
Streptococcus pyogenes C-203
Streptococcus viridans
Saccharomyces cerevisiae
Pityrosporium ovale
Microbicidal
Microbiostatic
1/3750
1/3
1/12
1/120
1/120
1/750
1/60
1/30
1/225
1/120
1/120
1/60
1/468.75
1/225
1/120
1/937.5
1/150
1/375
1/1500
1/750
1/1500
1/7500
1/6
1/12
1/240
1/7500
1/7500
1/7500
1/240
1/750
1/60
1/30
1/225
1/120
1/240
1/240
1/937.5
1/225
1/120
1/15000
1/300
1/375
1/3000
1/1500
1/3000
These data show that anti-microbe possesses a broad spectrum of effectiveness against a
variety of both gram-positive and gram-negative organisms.
What is Benzalkonium Chloride Based Foaming Sanitizer (BAC)?
Foaming BAC sanitizer was originally designed for the hands of professional health care providers who have
frequent contacts with people who are ill. Additionally, instant hand sanitizers provide effective hand hygiene
when washing is inconvenient, or facilities aren’t available. The United States Centers for Disease Control and
Prevention (CDC) states that the best way to prevent the spread of germs is hand washing and hand sanitizing.
Foaming BAC sanitizer instantly kills germs, helping to prevent the spread of disease. Foaming BAC sanitizer
helps stop germs wherever you are …at work, home, traveling, shopping, or anywhere that you may go. Unlike
alcohol based products, which may contains as much as 70%alcohol, Foaming BAC sanitizer is alcohol free and
non-flammable. You will see Foaming BAC sanitizer used at businesses, medical clinics, food preparation
services, airlines and in schools; any place where people gather and when hygiene and fire safety are primary
concerns. Foaming BAC sanitizer provides more than three times the number of applications of comparable size
alcohol gel sanitizers. However, it is gentle enough for daily use even on the sensitive skin of children.
What is the proper way to wash your hands?
Step 1:Make sure the paper towel is ready so you won ’t have to touch the dispenser
after your hands are already clean.
Step 2:Turn on the water and get your hands wet.
Step 3:Get some soap and wash your hands vigorously. Remember to get between your fingers and under your
fingernails. Keep washing for 10-15 seconds
Step 4:Then rinse your hands under the warm water until the soap is completely gone.
Step 5:Grab your paper towel and thoroughly dry your hands.
Step 6:Use your paper towel to turn off the water faucets.
Step 7:Finally,use your paper towel to open the restroom door and then put your paper towel in the nearest
trashcan.
When and where should I use Foaming BAC sanitizer?
Anytime you need to clean your hands, for example, before eating, after sneezing or coughing, when handling
money, after changing diapers, and after handling pets. Use Foaming BAC sanitizer anytime soap and water are
inconvenient, or unavailable.
How do I use Foaming BAC sanitizer?
Apply as directed, and use the same technique to rub in Foaming BAC sanitizer as you do to thoroughly wash
your hand with soap. Be sure to cover the palms, back of hands, between fingers, around the fingernails, and
clean under the fingertips by rubbing against the palm of the opposite hand.
What are the advantages of an Alcohol-Free sanitizer?
BAC Instant Hand Sanitizer is alcohol free and therefore has none of the ill effects associated with alcohol-gel
sanitizers. Research from several studies has concluded that the frequent use of alcohol based sanitizers actually
increases the number of bacteria on the skin. Alcohol gels and rubs strip away the skin’s natural oils and severely
dry the skin with repeated use. This effect has many negative implications that reduce the effectiveness of alcohol
based products in real world environments. For example, users of alcohol based products often complain of the
drying and irritation resulting from prolonged use of alcohol based products, and are therefore prone to avoid
their repeated use. Additionally, alcohol based products not only dry the skin, but also cause the formation of
small cracks in the skin increasing the skin ’s capacity to trap and carry disease-causing germs. Alcohol begins
evaporating from the skin almost immediately after it is applied. Upon evaporation of the alcohol, its germ-killing
power is gone, leaving the skin dry, chapped, and susceptible to infection. Alcohol based sanitizers contain as
much as 70%ethyl alcohol, and are therefore highly flammable. In contrast, Foaming BAC sanitizer contains no
alcohol and is non-flammable. Today, Foaming BAC sanitizer is present in commercial passenger aircraft,
elementary schools, public recreation areas, and many other environments where fire safety, as well as hygiene
are primary concerns. Unlike alcohol, Foaming BAC sanitizer will not sting or burn when it comes in contact with
small cuts and scrapes.
What is the germ-killing ingredient in BAC?
BAC Instant Hand Sanitizer contains the germ-killing ingredient Benzalkonium (ben-zal-coney-um) Chloride, or
BAC. This antiseptic has been used for over 60 years in hospitals and doctors offices. It is approved for safety
and effectiveness in antiseptic applications ranging from skin treatments for minor irritations (e.g. Bactine ®)to
surface sanitizers (e.g. Lysol®).It has even been approved by the FDA for use as a germ-killing ingredient in
mouthwashes.
If I can still smell the hand sanitizer does that mean it is still working?
Once an alcohol-based sanitizer has evaporated, its killing power is gone. Some brands of alcohol-based
sanitizers add heavy fragrance to the formulas to try and give a sense of security to the unwary consumer...(“as
long as I can smell it, it must still be working ”)...however, as soon as the alcohol has evaporated, it is no longer
killing germs. Foaming BAC sanitizer, when absorbed, leaves no detectable fragrance from perfumes because it
contains none. However, Foaming BAC sanitizer has a proven persistence of activity, which means it continues
to work, with repeated use, even after it is absorbed.
Why does skin become softer with repeated use of BAC?
Foaming BAC sanitizer is formulated to be highly beneficial to the skin. It does not break down the skin’s natural
water barrier, as do alcohol-containing products ;instead, Foaming BAC sanitizer protects that barrier and helps
preserve the skin ’s natural oils.
Does Foaming BAC sanitizer work better with repeated use? Why?
Yes. Foaming BAC sanitizer performance not only exceeds FDA standards, but its effectiveness actually
improves with repeated use. This unique formula does not contain alcohol, and will not dry or irritate the skin. The
germ-killing action of Foaming BAC sanitizer is persistent, which means its effect continues even after the
product has been absorbed into the hands.
Alcohol based sanitizers have emollients. Do they work as well as Foaming BAC sanitizer to keep skin
soft and smooth?
Repeated use of alcohol based hand sanitizers leaves the skin dry and actually more susceptible to invasion by
disease-causing germs. Some makers of alcohol sanitizers have added emollients, such as Aloe Vera, to try and
reduce the drying effect of the alcohol. However, the emollients remain on the skin after the alcohol has
evaporated, and these emollients become sticky. The remaining emollient is sticky, unpleasant to feel, and can be
transferred to food. The sticky emollient residue can actually become a “germ magnet” and collect germs from
surrounding surfaces. A better solution for disinfecting is the unique germ-killing, alcohol-free formula found in
BAC that leaves the skin cleaner and softer with repeated use.
Is Foaming BAC sanitizer supposed to be mixed with water or diluted before use?
No. Foaming BAC sanitizer is designed to be used as is – right from the bottle – anywhere, anytime.
Is Foaming BAC sanitizer safe to use right before eating?
Absolutely. Foaming BAC sanitizer is meant to be left on the skin until it is absorbed, usually 15 to 20 seconds.
Used as directed, Foaming BAC sanitizer will not transfer to food.
The National Food Safety Database section on hand sanitizers requires that “an antimicrobial product applied to
the hands is both safe and effective when applied to human skin; and that it be a safe food additive when applied
to bare hands that will come into direct contact with food.” Foaming BAC sanitizer meets these requirements.
What can I do as a parent, teacher, school health professional?
1)Set a good example, and let your children see you practice good hand hygiene
2)Reinforce the importance of hand hygiene at home and in the classroom
3)Contact your school principal to discuss: the impact of illness absenteeism on academic performance and
school funding & the availability of hand washing facilities, and implementing use of Foaming BAC sanitizer
alcohol-free, non-flammable sanitizer with Simple As ABC Hand Hygiene Program.