Terms you should know:
sterilization
disinfectant
pathogen
antiseptic
bactericidal (microbicidal)
bacteriostatic (microbiostatic)
germicidal
denature
autoclave
Questions you should be able to answer:
• What is the difference between sterilization and disinfection?
• What are some physical methods that could be used for sterilization?
• Are any chemical methods capable of sterilization? Which ones?
• What are some commonly used antiseptics? How do they work?
• What are two germicidal agents in a typical antibacterial dishwashing liquid?
Lecture outline:
I. Some definitions:
A. Sterilization: complete destruction of all microorganisms
B. Disinfection: reduction in numbers of pathogens to reduce danger of disease
C. Antiseptic: treatment used to reduce microorganisms on living tissue
D. Bactericidal (microbicidal, germicidal): kills bacteria (and/or other microorganisms)
E. Bacteriostatic (microbiostatic): prevents growth of bacteria (and/or other microorganisms)
II. Physical methods of control:
!
6
7
H
8
?
8
U
?
D
=
9
<
<
9
9
=
F
8
8
X
Z
$
%
&
#
'
%
(
=
=
C
B
C
B
N
O
P
Q
=
B
C
=
A
=
C
J
B
*
$
+
,
-
8
;
9
7
<
=
;
:
8
;
9
7
<
=
B
:
8
;
9
7
<
=
;
R
%
=
$
%
>
)
8
F
/
?
9
=
.
8
>
@
9
=
8
;
9
9
=
;
F
9
<
7
C
J
9
8
;
9
7
<
=
;
:
8
;
9
7
<
=
B
K
:
=
?
?
>
8
F
8
9
=
B
<
=
?
I
=
A
0
8
F
9
=
0
J
#
B
<
%
1
B
D
I
7
<
=
?
=
A
8
9
=
B
C
@
9
7
<
=
?
=
A
8
9
=
B
C
9
9
K
@
G
=
F
=
C
Y
7
;
9
=
B
C
D
=
F
=
C
Y
7
;
9
=
B
C
B
F
F
=
V
?
#
7
C
?
M
D
T
Y
Y
7
7
;
9
8
;
=
7
5
=
8
;
?
<
7
C
0
C
.
%
)
4
'
<
F
7
8
;
9
=
B
C
F
T
L
<
9
S
F
9
B
=
L
7
4
<
D
F
F
&
L
F
J
3
G
7
7
8
)
F
K
W
L
'
7
J
B
8
<
;
8
L
$
E
F
L
7
2
9
I
8
7
.
8
B
D
D
;
.
C
@
?
9
7
@
C
J
L
)
:
>
>
=
#
9
B
M
"
B
B
L
F
<
G
V
B
J
<
<
9
8
8
C
C
Q
=
7
7
9
F
L
F
F
;
[
III. Chemical methods of control:
(
H
K
6
?
X
T
;
b
<
9
K
X
?
J
#
&
\
7
B
<
?
7
J
G
;
C
B
7
8
=
=
K
9
B
`
<
B
7
@
<
>
?
D
B
2
=
C
N
C
B
9
7
.
7
=
$
>
G
F
7
)
9
K
<
C
B
8
+
(
7
^
C
=
B
>
*
$
+
,
-
%
$
%
)
.
/
?
F
7
C
8
^
=
>
7
N
J
F
R
%
1
8
?
@
9
7
<
=
?
=
A
8
9
=
B
C
G
B
F
F
=
V
?
7
]
:
8
;
9
7
<
=
;
=
>
8
?
@
9
7
<
=
?
=
A
8
9
=
B
C
G
B
F
F
=
V
?
7
D
8
L
8
J
7
F
D
:
8
;
9
7
<
=
;
=
>
8
?
D
=
F
=
C
Y
7
;
9
=
B
C
D
8
L
8
J
7
F
L
:
8
;
9
7
<
=
;
=
>
8
?
D
=
F
=
C
Y
7
;
9
=
B
C
:
<
D
=
F
=
C
Y
7
;
9
=
B
C
D
=
F
=
C
Y
7
;
9
=
B
C
9
7
<
=
;
=
>
8
?
:
8
;
9
7
<
=
;
=
>
8
?
@
9
7
<
=
?
=
A
8
9
=
B
C
=
G
a
B
F
F
=
V
?
7
>
K
]
C
8
9
=
C
G
=
W
8
9
X
7
?
F
=
4
C
A
S
7
X
F
7
=
L
L
=
L
>
<
F
<
X
A
X
<
8
L
C
B
C
<
?
%
)
4
'
F
G
V
8
.
7
V
;
C
5
\
7
G
0
T
L
K
B
&
7
G
K
3
F
E
G
9
7
F
F
;
8
_
F
B
8
W
)
>
7
=
'
=
;
9
$
;
T
;
2
=
8
8
.
<
:
C
#
7
Q
8
.
9
F
B
#
;
C
7
0
8
F
B
0
:
7
?
)
9
8
7
9
7
F
7
F
F
B
K
C
Q
7
L
=
F
C
7
7
F
W
=
8
F
?