Drug Discovery
C
A3
H
EP
1
B
H
EP
A6
EP
A7
H
EP
B
H
IR
AK
AK
2
D 3) )
N KR 88
K ) 4 (A G K2
K1
R IP K 3 (R IC RI PK 1 (S
KK
R IPIP K 2
AN
R
4
L
F)
AF
BR F1 (c RA
RA
1)
(K SR
KS RR2
KS
MA P3
MA P3
49 8)
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KIA A12)
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(M
K9
LK
MA P3 K11 (M
MA P3
4)
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ZA K
K1)
K7 (TA
MAP 3K7
(ASK 1)
MAP 3K5
MAP3 K6
CMGC
MAP3 K4
3074 )
3)
YSK4 (FLJ2
MAP3 K3 (MEKK
2)
MAP3K 2 (MEKK
MAP3K1
MAP3K14 (NIK)
MAP3K8 (COT)
EIF2AK4_D2 (GCN2_D2)
MAP2K1 (MEK1)
MAP2K2 (MEK2)
MAP2K5
MAP2K3 (MEK3)
MAP2K6 (MKK6)
STE
MAP2K4
MAP2K7
LYK5 (STLK5)
ALS2CR2
OXSR1 (OSR1)
STK39 (STLK3)
PAK4
PAK7
PAK6
PAK1
PAK3
PAK2
TAOK 2
(TAO
TAOK 1 (MAR 1)
(JIK )
KK, PSK2
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NE K8
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MAST1
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PL K 2
AU RK
PL
AU A)
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NE K6
NE K7
NE K1
1
NE
K4
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AK SG 3
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ga
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RP S6 RP S6 S6 K
4)
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(p
(P
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K)
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2
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PD
TT
10 )
(N EK
26 85 SE D)
FL J3 6 (FU
ST K3
UL K4
K1)
)
AA K1 K (BI KE
BM P2
)
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STK 35L (CL IK1 K0 71)
PD IK1 F96 (SG
C9 ORK1 (KIS )
UHM
1)
gam ma 2)
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ma
CSN K1G (CK1 gam
CSN K1G2 gam ma 3)
(CK1
CSN K1G3
VRK3
VRK1
VRK2
TTBK1
TTBK2
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2)
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CSNK1D (CK1 delta)
CSNK1E (CK1 epsilon)
3)
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PS
6 (M
ST K1 GA K
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3
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K49
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STK3 8L
(NDR 2)
STK3 8 (NDR1
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ROCK 1
ROCK2
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CDC42B PG (DMPK2) (DMPK1)
CDC42BP B (MRCK
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GR K7RB K1
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RK1)
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GR K4 (GP RK 5) (GP RK )
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4)
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BR RD2
B
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(ZC 4) TN IK
MY O3
A
MY O3
B
AT
CD K10
C
K1 0
K (HH
TN NI3
LIM K2
LIM K1
TES K1
TES K2
CD K7
AB
LK1))
3)
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(D LK
K)
K1 2
MA P3 K1 3 (LZ
MA P3
STK 24
(MS T3)
STK 25
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STK 4 (MS T2)
(MS T1)
HE D)
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2
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TG 7)
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1
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6
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2
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MAPK6 (ERK3)
CDK3
CDK2
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(H
3
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K g
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2 (P
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CDK L4
CDK L1
CDKL 2
CDKL 3
2)
(PCT AIRE 1)
PCTK 2
(PCT AIRE (PCT AIRE 3)
PCTK 1
1)
PCT K3
(PFT AIRE 2)
PFT K1
AIRE
7 (PFT
CDK 8)
ALS 2CR
(CD K11
CDC 2L6
TSL RE)
L1 (PI C2 L2
CD
CD C2
E
PH
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EP PH
E
GS K3
GSK 3A B (GS K3 PR PF4 B
(PR P4
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MAK
ICK
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(MO K)
NLK
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AA 10 6)
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3 (L LM TK (L
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M R3 TK
M R1
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R2
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DD
R
DD 1
MU R2
SK
YK
TK
ST
LM
2)
ALK
(RO S)LTK
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TEK (TIE
IGF 1R
INS
(IR R) R
FGFR1
FGFR2
FGFR3
FLT3 (VEG
FR3)
CSF 1R
(FMS )
PDG FRA KIT (cKI T)
PDG FRB (PDG FR alph
a)
(PD GFR
bet a)
FLT1 (VEGF
KDR (VEGF R1)
R2)
FGFR4
FLT4
FRK (RAK, GTK)
(ARG )
BMX
BTK
ITK
ABL 2
A XL
TY RO
3 (D ME RT
ME
K (M
MS T (c ME tk )
ER )
T1
RY K R (R T)
TN K2
ON )
TN K1
(A CK
)
TT N
TR AD
TR IO
1)
5
K
EL
M RK )
SN IM1
(N
10
HK
42
1 (C
SK
EK
GC
RO S1
CH
PA
M
INS RR
2
(L KB
4
RET
K1 1
SK
PTK6 (BRK)
SRMS (SRM)
CSK
MATK (HYLTK, CTK)
FER (T YK3)
FES (FPS)
TEC
TXK
BLK
FYN
HCK
LYN
ST
TS
P IM
)
(SP EGN
APE G1 OB SC )
_D2
2
(SP EG
1_D 2 OB SC N_D
)
ABL 1 (ABL
AP EG
3
P IM 1
P IM
5)
R K K)
R
1 (A NA
A K (S
N U K2
A
NU
5)
LC K)
LC K) (S MM
GK 08 K)
6 (S
LC
(S KMMY LK
40 15 (C AM LK 2
MY
LO C3 ML CK
2
3
PK
DA PK
DA K2 )
PK1
DA
(D RA K1)
7B (D RA
ST K1 7A
K1
ST
1)
D)
22
B)
TK
22
1 (S
SK (S TK SS TK
TS
2
K 3)
SK
SS
TS
C (T
K 22
ST
4
RK
MA
2
RK
)
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)
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IK
(Q K2
(Q
)
9
S
K2 (SIK 99 BR S K1
F1L K IAA0
B R KA1) )
F1L K
SN
MP A2
(A PK
M
A1
KA 2 (A
PR AA
K
PR
C
CA AM
M K4
KV
R
CA
RP PS6
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AC
SK
KA 1_D
A
3_D 2
P MK
2 (RS
D P SK L)
RP (RS K1_
D C CA SKH H1
S6 K2 D2
A M MK 2
KA _D )
K L L1
RP
2_D 2)
2
RP S6
2
K
(R
S6 A
RP S
DC
KA 4_D
S6 K3
AM
5_D 2
KA _D
(M
K
6_D 2)
2
L3
(M SK
2
SK 2_D
(R
SK
1_D 2)
4_D
2)
2
M )
MK KNK
NK 1 (M
2
(M NK
M M A NK 1)
M AA P K P K A 2)
PK AP PK
A P K 3 5 (P
K2
RA
K)
HI PK
PR
HI PK 2
1
PR K D 1
HI PK
K D (P
3 (E K C
3 (D
PK M ) C H EK
YR K6
DY RK
2 (C
)
P R 2)
DY RK 3
KD
HK
2 (P
2
2)
HI PK
KD
4
2)
DY RK
DY RK 4
DY RK 1A
1B
CL K1
CL K4
CL K3
CL K2
SR PK
SR PK 1
2
ST K2
3 (MS
SK1)
MAPK1 4 (p38
alpha)
MAPK11 (p38
beta)
MAPK13 (p38
delta)
APK12 (p38 gamma)
MAPK8 (JNK1)
MAPK10 (JNK3)
MAPK9 (JNK2)
MAPK1 (ERK2)
MAPK3 (ERK1)
MAPK7 (ERK5)
DC
LCK
3
RK 1
RK
SN
C C
AM A
M
C K K
C AM 2G 2B
AM K
PN
(C (C
K 2D A A
CK
2
A (C M K M K
(C
(C A II II
AM
AM MK g b
KI
m
K II a m e t a
m
II d e m )
b
a)
eta
a lp lt a)
)
h a a)
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FGR
MA
MA
C
CA AM
M K1
K1
(C
D
(C A M
CA AM KI
M K I a lp
K1 d h
G e lt a)
(C a
AM )
KI
ga
SRC
YES1 (YES)
Kinase protein and assay
comparison guide
N
K
2
FL
IN
)
J2
33
56
(S
GK
19
6)
PA
SC CE
YL -1
(S
1
C
YL
3)
BU
LO B1B
(B
PX C9
K 146 UB
R
(S
LO 1 (S 1)
B) GK
49
TP
3)
NR 53R
BP K
(P
2
RP
K)
G UC
G UC Y2 D
(C YG
N PR Y2 F
D
(C
N PR 1 (A N YG F) )
2 (A PA )
N PB
)
Drug Discovery
Boost your kinase research
Discover the most comprehensive set of proteins and assay technologies
→ Access our collection of more than 300 high-quality, ready-to-use, purified human kinases
→ Choose from 6 preconfigured biochemical fluorescence assay solutions
→ Analyze complex signal transduction pathways in live cell–based assays
Contents
Purified human kinase overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Kinase protein and assay comparison table . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Biochemical kinase assay technology overview and comparison guide . . . . . . . . . . . . . . . . . . . . . . . . . . . .34
LanthaScreen® activity assays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .36
LanthaScreen® Eu Kinase Binding Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38
Adapta® Universal Kinase Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40
Z´-LYTE® FRET Assays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42
Omnia® real-time kinase assays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42
SelectScreen® Kinase Profiling Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43
Cellular pathway analysis assay comparison guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44
CellSensor® cell lines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45
LanthaScreen® cellular assays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46
SelectScreen® Cell-Based Pathway Profiling Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47
Custom services . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .48
On front cover:
This circular diagram represents an artistic interpretation of the phylogenetic relationships among all members of the human kinase family. Our bioinformatics analysis
concluded that the superfamily comprises 517 distinct protein kinases (including the 14 enzymes containing two kinase domains; 531 catalytic domains in all). The kinases are
organized into nine major groups (TK, TKL, STE, CK1, AGC, CMGC, CAMK, Other, and Atypical). The nine major groups are based upon the similarity between the amino acid
sequences of the enzyme catalytic domains, with two exceptions. The three-dimensional structure in the middle of the diagram is a model based on cAMP-dependent protein
kinase. To request a Human Kinome poster for your lab, visit www.invitrogen.com/kinasebiology.
2
Purified human kinase overview
A
Quality and validation are the primary characteristics of our
purified recombinant human kinases. This focus allows for consistency and reliability—critical factors for disease research and
drug discovery. We have established a strictly controlled validation process to maintain target consistency and reproducibility
(Figure 1).
Each enzyme is:
Sequence validated prior to expression
→
Expressed according to strictly controlled processes
→
Evaluated by SDS-PAGE for purity
→
Identity confirmed by mass spectrometry
→
Assessed for activity in a radiometric phosphorylation assay
Our kinase target portfolio is designed to meet your specific
0.08
B
0.07
Activity (nmol/min)
→
0.06
0.05
0.04
0.03
0.02
needs. We provide prepacked sample vials for small-scale stud-
0.010
ies or assay development and provide larger quantities to sup-
0
port lead discovery programs. To view the most current listing of
0
10
20
30
40
50
60
ng protein/30 μL assay
kinase proteins available,* visit www.invitrogen.com/kinases.
C
Figure 1. Sample validation data for IKBKE (IKKe). Invitrogen kinases have the Human Genome Nomenclature Committee (HGNC) name in the first position and the “common” name in parentheses where appropriate. (A) Analysis of protein preparation by SDS-PAGE. Left to right lanes: BenchMark™ Protein Ladder; 0.5 μg, 1 μg, 2.5 μg,
and 5 μg IKBKE (IKKe). (B) Activity assessment of IKBKE (IKKe) in radioactive assay format. (C) Sequence alignment of Invitrogen construct with the GenBank® entry.
These sequences are 100% identical. For other proteins, differences in the sequences would be indicated by boxes around the differing residues.
* To inquire about unactive kinases available, email us at discoverysciences@invitrogen.com.
3
www.invitrogen.com
Drug Discovery
Kinase protein and assay comparison table
Kinase
Aliase(s)
1 mg
Cat. No.
Accession number
Length
Expression
Tag
Classification
SelectScreen®
Kinase Profiling
Service
Z’-LYTE® assay
Adapta® assay
Assay kit
Substrate
ABL1
ABL, c-ABL
P3049
PV3585
NP_005148.2
Full
Insect
C-terminal His
Cytoplasmic
tyrosine kinase
✓
PV3191
Tyr 2 Peptide
ABL1 E255K
ABL, c-ABL
PV3864
PV4182
NP_005148.2, E255K Full
Insect
C-terminal His
Cytoplasmic
tyrosine kinase
✓
PV3191
Tyr 2 Peptide
ABL1 G250E
ABL, c-ABL
PV3865
PV4183
NP_005148.2, G250E Full
Insect
C-terminal His
Cytoplasmic
tyrosine kinase
✓
PV3191
Tyr 2 Peptide
ABL1 T315I
ABL, c-ABL
PV3866
PV4184
NP_005148.2, T315I
Full
Insect
C-terminal His
Cytoplasmic
tyrosine kinase
✓
PV3191
Tyr 2 Peptide
ABL1 Y253F
ABL, c-ABL
PV3863
PV4181
NP_005148.2, Y253F Full
Insect
C-terminal His
Cytoplasmic
tyrosine kinase
✓
PV3191
Tyr 2 Peptide
ABL2
Arg
PV3266
PV3586
NP_009298.1
Full
Insect
C-terminal His
Cytoplasmic
tyrosine kinase
✓
PV3191
Tyr 2 Peptide
ACVR1
ACTRI, ACVRLK2,
ALK2, FOP, SKR1
PV4877
PV4878
NP_001096.1
Catalytic
(145–509)
Insect
N-terminal GST Serine/
threonine
kinase
✓
ACVR1B
ALK4, SKR2, ARTRIB, PV4312
ACVRLK4
PV4329
NP_004293
Catalytic
(150–505)
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3802
Ser/Thr 16
Peptide
ACVR2B
ActR-IIB, ACTRIIB
PV6049
PV6050
NP_001097.3
Catalytic
(185–488)
Insect
N-terminal GST Serine/
threonine
kinase
ACVRL1
ACVRLK1, ALK-1,
ALK1, HHT, HHT2,
ORW2, SKR3, TSR-I
PV4883
PV4884
NP_000011.2
Catalytic
(139–503)
Insect
N-terminal GST Serine/
threonine
kinase
ADRBK1
GRK2, BARK1, BETA- PV3361
ARK1
PR4694A
NP_001610.2
Full
Insect
C-terminal His
Serine/
threonine
kinase
✓
PV3802
Ser/Thr 16
Peptide
ADRBK2
GRK3, BARK2
PV3827
PV3974
NP_005151.2
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3802
Ser/Thr 16
Peptide
AKT1
PKB alpha, RAC,
PRKBA
P2999
PV3599
NP_005154
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV3179
Ser/Thr 6
Peptide
AKT2
PKB beta, PRKBB,
RAC beta
PV3184
PV3975
NP_001617
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV3179
Ser/Thr 6
Peptide
AKT3
PKB gamma, PRKBG, PV3185
RAC-gamma
PV3976
NP_005456
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV3179
Ser/Thr 6
Peptide
PV3867
PV4185
NP_004295.2,
J1461V
Cytoplasmic
(1058–1620)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3190
Tyr 1 Peptide
PV4672
PV4673
PRKAA1:
NP_006242.4,
PRKAB1:
NP_006244.2,
PRKAG1:
NP_002724.1
Full, full, full
Insect
N-terminal
GST,
N-terminal
GST,
N-terminal His
✓
PV4644
Ser/Thr 23
Peptide
ALK
AMPK (A1/
B1/G1)
4
10 μg
Cat. No.
PRKAA1/PRKAB1/
PRKAG1
Serine/
threonine
kinase
LanthaScreen® Tb activity assay
Antibody
Substrate
LanthaScreen® Eu activity assay
Antibody
Substrate
LanthaScreen® Eu Kinase Binding
Assay
Antibody
Omnia® assay kit
Tracer
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV6088
Tracer 1710
KNZ3061
Y6 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV6088
Tracer 1710
KNZ3061
Y6 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV6088
Tracer 1710
KNZ3061
Y6 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV6088
Tracer 1710
KNZ3061
Y6 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
KNZ3061
Y6 Peptide
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV5994/PV5995
Eu-anti-GST
PV5593
Tracer 178
SelectScreen®
Cell-Based
Pathway Profiling
Service
LanthaScreen® cellular assay
CellSensor®
cell line
KNZ1011
ST1 Peptide
✓
K1468 T-REx™
FOXO3-DBE HeLa
PV3564/PV3565
Tb-Crosstide
PV3509
Fl-Crosstide
KNZ1011
ST1 Peptide
✓
K1468 T-REx™
FOXO3-DBE HeLa
PV3564/PV3565
Tb-Crosstide
PV3509
Fl-Crosstide
KNZ1011
ST1 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV3566 Tb-pCREB PV3508
pSer133
Fl-CREBtide
K1528
PRAS40 HEK293E
PV5102/PV5103
PRAS40 [pSer246]
K1514
SBE-bla A375
PV3509
Fl-Crosstide
PV5693/PV5863
AF647-GT
Antibody
KNZ3061
Y6 Peptide
PV3564/PV3565
Tb-Crosstide
PV5692/PV5696
Eu-PY-20
Cell line
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ3041
Y4 Peptide
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1231
ST23 Peptide
Inquire
✓
K1667
NFkB ME-180
* To inquire about LanthaScreen® cellular assays available for these kinases, email us at discoverysciences@invitrogen.com.
5
www.invitrogen.com
Drug Discovery
Kinase
Aliase(s)
1 mg
Cat. No.
Accession number
Length
Expression
Tag
Classification
SelectScreen®
Kinase Profiling
Service
Z’-LYTE® assay
Adapta® assay
Assay kit
Substrate
AMPK (A2/
B1/G1)
PRKAA2/PRKAB1/
PRKAG1
PV4674
PV4675
PRKAA1:
NP_006243.2,
PRKAB1:
NP_006244.2,
PRKAG1:
NP_002724.1
Full, full, full
Insect
N-terminal
GST,
N-terminal
GST,
N-terminal His
Serine/
threonine
kinase
✓
PV4644
Ser/Thr 23
Peptide
AURKA
Aurora A, AIK,
AURA, STK6
PV3612
PV4067
NP_940839
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV3174
Ser/Thr 1
Peptide
AURKB
Aurora B, AIK2,
ARK2
PV3970
PV4274
NP_004208
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV3174
Ser/Thr 1
Peptide
AURKC
Aurora C, AIK3,
STK13, AIE2
PV3856
PV4178
AAH75064
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV4529
Ser/Thr 19
Peptide
AXL
UFO
PV3971
PV4275
NP_068713
Cytoplasmic
(473–894)
Insect
C-terminal His
Receptor
tyrosine kinase
✓
PV4122
Tyr 6 Peptide
BLK
MGC10442
PV3683
PV4073
NP_001706
Full
Insect
N-terminal His
Cytoplasmic
tyrosine kinase
✓
PV3190
Tyr 1 Peptide
BMPR1A
ALK3
PV6038
PV6039
NP_004320.2
Catalytic
(187–532)
Insect
N-terminal GST Serine/
threonine
kinase
✓
BMX
ETK, PSCTK3,
PSCTK2
PV3371
PV4074
NP_001712
Full
Insect
C-terminal His
✓
PV3190
Tyr 1 Peptide
BRAF
BRAF1, RAFB1
PV3848
PV4172
NP_004324.2
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3176
Ser/Thr 3
Peptide
BRAF V599E
BRAF1, RAFB1
PV3849
PV4173
NP_004324.1, V599E Catalytic
(416–766)
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3176
Ser/Thr 3
Peptide
BRSK1
SAD1, SAD1A
PV4333
PV4344
NP_115806
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV4535
Ser/Thr 21
Peptide
BTK
ATK, XLA
PV3363
PV3587
NP_000052
Full
Insect
C-terminal His
Cytoplasmic
tyrosine kinase
✓
PV3190
Tyr 1 Peptide
PV4391
PV4427
NP_003647
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
CAMK1
6
10 μg
Cat. No.
Cytoplasmic
tyrosine kinase
PV5094
ZIPtide
CAMK1D
CaMKI delta, CLKiK
PV3663
PV3977
NP_705718
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV3667
Ser/Thr 10
Peptide
CAMK2A
CaMKII alpha,
CAMKA, KIAA0968
PV3142
PR4586A
NP_741960
Full
Insect
C-terminal His
Serine/
threonine
kinase
✓
PV3177
Ser/Thr 4
Peptide
CAMK2B
CaMKII beta,
CAMKB, CAM2,
CAMK2
PV4205
PV4278
NP_742078
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV4316
Ser/Thr 17
Peptide
CAMK2D
CaMKII delta
PV3373
PV3978
NP_742113
Full
Insect
C-terminal His
Serine/
threonine
kinase
✓
PV3177
Ser/Thr 4
Peptide
CAMK4
CaMKIV, CAMK-GR,
MGC36771
PV3310
PV3979
NP_001735
Full
E. coli
N-terminal GST Serine/
threonine
kinase
✓
PV3793
Ser/Thr 13
Peptide
CAMKK1
CAMKKA,
MGC34095,
DKF2p761M0423
PV4670
PV4671
NP_115670
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
CAMKK2
CaMKK beta,
CAMKK, MGC15254
PV4206
PV4322
NP_757380.1
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
LanthaScreen® Tb activity assay
Antibody
Substrate
LanthaScreen® Eu activity assay
Antibody
Substrate
LanthaScreen® Eu Kinase Binding
Assay
Antibody
Omnia® assay kit
Tracer
SelectScreen®
Cell-Based
Pathway Profiling
Service
✓
CellSensor®
cell line
LanthaScreen® cellular assay
Cell line
Antibody
PV3566 Tb-pCREB PV3508
pSer133
Fl-CREBtide
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1231
ST23 Peptide
PV3566 Tb-pCREB PV3508
pSer133
Fl-CREBtide
PV5596/PV5597
Eu-anti-His
PV5592
Tracer 236
KNZ1161
ST16 Peptide
Inquire*
Inquire*
PV3560/PV3561
Tb-pSer (pPKC)
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
KNZ1161
ST16 Peptide
Inquire*
Inquire*
K1798
ERK2 A375
PV5269/PV5270
ERK2 [pThr185/
pTyr187]
PV3506 Fl-PKC
K1667
NFkB ME-180
KNZ1161
ST16 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5596/PV5597
Eu-anti-His
PV5592
Tracer 236
KNZ3111
Y11 Peptide
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
KNZ3051
Y5 Peptide
PV5594/PV5595
Eu-anti-GST
PV5593
Tracer 178
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
PV4813/PV4814
Tb-pMAP2K1
PV4812
Fl-MAP2K1
PV5594/PV5595
Eu-anti-GST
PV5593
Tracer 178
PV4813/PV4814
Tb-pMAP2K1
PV4812
Fl-MAP2K1
PV5594/PV5595
Eu-anti-GST
PV5593
Tracer 178
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV5596/PV5597
Eu-anti-His
PV5592
Tracer 236
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV3566 Tb-pCREB PV3508
pSer133
Fl-CREBtide
KNZ3051
Y5 Peptide
K1490
NFAT RA-1
✓
K1667
NFkB ME-180
K1660
AP-1 A375
KNZ3051
Y5 Peptide
K1490
NFAT RA-1
KPZ1311
ST31 Peptide
PV3566 Tb-pCREB PV3508
pSer133
Fl-CREBtide
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
KNZ1231
ST23 Peptide
PV3564/PV3565
Tb-Crosstide
PV3509
Fl-Crosstide
PV5596/PV5597
Eu-anti-His
PV5592
Tracer 236
KNZ1231
ST23 Peptide
PV3566 Tb-pCREB PV3508
pSer133
Fl-CREBtide
PV5596/PV5597
Eu-anti-His
PV5592
Tracer 236
PV3564/PV3565
Tb-Crosstide
PV3509
Fl-Crosstide
PV5596/PV5597
Eu-anti-His
PV5592
Tracer 236
PV3566 Tb-pCREB PV3508
pSer133
Fl-CREBtide
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV5596/PV5597
Eu-anti-His
PV5592
Tracer 236
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1231
ST23 Peptide
7
www.invitrogen.com
Drug Discovery
Kinase
Aliase(s)
1 mg
Cat. No.
Accession number
Length
Expression
Tag
Classification
SelectScreen®
Kinase Profiling
Service
Z’-LYTE® assay
Adapta® assay
Assay kit
Substrate
CDC42BPA
MRCKa, MRCK,
PK428
PV4398
PV4434
NP_055641
Catalytic
(1–473)
Insect
C-terminal His
Serine/
threonine
kinase
✓
PV3793
Ser/Thr 13
Peptide
CDC42BPB
MRCKb
PV4399
PV4435
NP_006026.2
Catalytic
(1–473)
Insect
C-terminal His
Serine/
threonine
kinase
✓
PV3793
Ser/Thr 13
Peptide
CDK1/cyclin B
PV3292
PV3980
NP_001777,
NP_114172
Full
Insect
C-terminal His
Serine/
threonine
kinase
✓
PV3673
Ser/Thr 18
Peptide
CDK2/cyclin A
PV3267
PR4448A
NP_001789,
NP_001228
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV3673
Ser/Thr 12
Peptide
CDK4/cyclin D1 CDK4/CND1, CMM3, PV4400
MGC14458
PV4436
NP_000066,
NP_4444284
Full
Insect
N-terminal GST Serine/
threonine
kinase
CDK5/p25
CDK5R, MGC33831,
PSSALRE
PV4676
PV4677
NP_004926.1/
NP_003876
Full
Insect
N-terminal
Serine/
His/N-terminal threonine
GST
kinase
✓
PV3673
Ser/Thr 12
Peptide
CDK5/p35
PSSALRE
PV3000
PV3981
NP_004926.1/
NP_003876
Full
Insect
N-terminal His
✓
PV3673
Ser/Thr 12
Peptide
CDK6/cyclin D1 CDK6/CCND1,
PV4401
MGC59692, PLSTIRE
PV4437
NP_001250,
NP_444284
Full
Insect
N-terminal GST Serine/
threonine
kinase
CDK7/cyclin H/
MNAT1
PV3868
PV4186
NP_001790 (Q130R),
NP_001230,
NP_002422)
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
Serine/
threonine
kinase
CDK8/cyclin C
CDK8/CCNC, K35,
MGC126074
PV4402
PV4345
NP_001251,
NP_005181
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
CDK9/cyclin K
CDK9:TAK,
C-2K, DCD2L4,
cyclinK:CCNK
PV4335
PV4346
NP_001252
NP_003849
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV4131
PV4280
NP_001252
NP_001231
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
CDK9/cyclin T1
8
10 μg
Cat. No.
PV5090
CDK7/9tide
PV5090
CDK7/9tide
CHEK1
CHK1
P3040
PV3982
NP_001265
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV4529
Ser/Thr 19
Peptide
CHEK2
CHK2, CDS1
PV3367
PV3983
NP_009125
Full
Insect
C-terminal His
Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
CHUK
IKK-alpha, Chuk1,
IKK1
PV4310
PV4327
NP_001269
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
CLK1
PV3315
PV3984
NP_004062
Catalytic
(129–484)
E. coli
N-terminal GST Serine/
threonine
kinase
✓
PV3324
Ser/Thr 9
Peptide
CLK2
PV4201
PV4279
NP_003984
Catalytic
(137–498)
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3179
Ser/Thr 6
Peptide
CLK3
PV3826
PV3985
NP_003983, T132S,
G133S
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV4319
Ser/Thr 18
Peptide
CLK4
PV3839
PV3986
NP_065717
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
No substrate
required
LanthaScreen® Tb activity assay
Antibody
Substrate
LanthaScreen® Eu activity assay
Antibody
Substrate
LanthaScreen® Eu Kinase Binding
Assay
Antibody
Omnia® assay kit
Tracer
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
KNZ1181
ST18 Peptide
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
KNZ1181
ST18 Peptide
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
KNZ1071
ST7 Peptide
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
KNZ1071
ST7 Peptide
SelectScreen®
Cell-Based
Pathway Profiling
Service
LanthaScreen® cellular assay
CellSensor®
cell line
Cell line
Antibody
K1639
dhfr(E2F) NIH3T3
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1071
ST7 Peptide
KNZ1071
ST7 Peptide
PV3566 Tb-pCREB PV3508
pSer133
Fl-CREBtide
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
PV5596/PV5597
Eu-anti-His
PV5592
Tracer 236
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
PV3566 Tb-pCREB PV3508
pSer133
Fl-CREBtide
PV3562/PV3563
PV3507 Fl-IKK
Tb-I kappa B alpha
pSer32
✓
K1676
LEF/TCF HCT116
✓
K1667
NFkB ME-180
KNZ1111
ST11 Peptide
KNZ1031
ST3 Peptide
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1151
ST15 Peptide
KNZ1151
ST15 Peptide
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
9
www.invitrogen.com
Drug Discovery
Kinase
CSF1R
Aliase(s)
FMS, CSFR, c-FMS,
CD115
CSK
1 mg
Cat. No.
Accession number
Length
Expression
Tag
Classification
SelectScreen®
Kinase Profiling
Service
Z’-LYTE® assay
Adapta® assay
Assay kit
Substrate
PV3249
PV4092
NP_005202
Cytoplasmic
(538–910)
Insect
C-terminal His
Receptor
tyrosine kinase
✓
PV3190
Tyr 1 Peptide
P2927
PV4075
NP_004374
Full
E. coli
C-terminal His
Cytoplasmic
tyrosine kinase
✓
PV3191
Tyr 2 Peptide
CSNK1A1
CK1, HLCDGP1,
PRO2975
PV3850
PV4174
NP_001883, K164Q
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3670
Ser/Thr 11
Peptide
CSNK1D
CK1delta, HCK1D
PV3665
PV3987
NP_620693
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3670
Ser/Thr 11
Peptide
CSNK1E
CK1epsilon, HCKIE
PV3500
PV3988
NP_001885
Full
Insect
C-terminal His
Serine/
threonine
kinase
✓
PV3670
Ser/Thr 11
Peptide
CSNK1G1
CK1 gamma 1
PV3825
PV3989
NP_071331
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3181
Ser/Thr 5
Peptide
CSNK1G2
CK1 gamma 2
PV3499
PV3990
NP_001310
Full
Insect
C-terminal His
Serine/
threonine
kinase
✓
PV3181
Ser/Thr 5
Peptide
CSNK1G3
CK1 gamma 3
PV3838
PV3991
NP_004375, R174G
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3181
Ser/Thr 5
Peptide
CSNK2A1
CK2 alpha 1, CKII,
CK2A1
PV3248
PV3992
NP_001883.4
Full
Insect
C-terminal His
Serine/
threonine
kinase
✓
PV3670
Ser/Thr 11
Peptide
CSNK2A2
CK2 alpha 2
PV3624
PV3993
NP_001887
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3670
Ser/Thr 11
Peptide
DAPK1
DAPK
PV3969
PV4273
NP_004929
Catalytic
(1–363)
Insect
N-terminal His
Serine/
threonine
kinase
✓
DAPK2
DRP-1
PV3614
PV3994
NP_055141
Full
Insect
N-terminal His
Serine/
threonine
kinase
DAPK3
ZIPK, ZIP
PV3686
PV3995
NP_001339
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3793
Ser/Thr 13
Peptide
DCAMKL2
DCK2, DCDC3,
MGC45428
PV4297
PV4323
NP_689832
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV4316
Ser/Thr 17
Peptide
PV6047
PV6048
NP_001945
Catalytic
(440–876)
Insect
N-terminal GST Serine/
threonine
kinase
DDR1
10
10 μg
Cat. No.
PV5094
ZIPtide
DDR2
TKT, NTRKR3
PV3870
PV4188
NP_006173, S642A
Cytoplasmic
(427–855)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
DMPK
DM
PV3784
PV3996
NP_004400
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
DNAPK
PRKDC
PV5866
PV5865
Endogenous
Full
MOLT4
None
Serine/
threonine
kinase
✓
DYRK1A
DYRK1, DYRK,
MNBH, HP86
PV3785
PV3997
NP_001387
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV4319
Ser/Thr 18
Peptide
DYRK1B
MIRK
PV4649
PV4650
NP_004705
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV4319
Ser/Thr 18
Peptide
DYRK3
PV3837
PV3998
NP_003573
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3324
Ser/Thr 9
Peptide
DYRK4
PV3871
PV4189
NP_003836.1
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3324
Ser/Thr 9
Peptide
LanthaScreen® Tb activity assay
Antibody
Substrate
LanthaScreen® Eu activity assay
Antibody
Substrate
LanthaScreen® Eu Kinase Binding
Assay
Antibody
Omnia® assay kit
Tracer
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5596/PV5597
Eu-anti-His
PV5592
Tracer 236
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
SelectScreen®
Cell-Based
Pathway Profiling
Service
CellSensor®
cell line
LanthaScreen® cellular assay
Cell line
Antibody
KNZ3091
Y9 Peptide
KNZ1241
ST24 Peptide
KNZ1241
ST24 Peptide
KNZ1241
ST24 Peptide
KNZ1241
ST24 Peptide
KNZ1241
ST24 Peptide
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1241
ST24 Peptide
KNZ1221
ST22 Peptide
KNZ1011
ST1 Peptide
KNZ1011
ST1 Peptide
✓
K1661
AP-1 ME-180
KNZ1041
ST4 Peptide
PV3552/PV3553
Tb-PY-20
PV5130/PV5131
Tb-phospho-p53
[pSer15]
PV3560/PV3561
Tb-pSer (pPKC)
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5994/PV5995
Eu-anti-GST
PV5593
Tracer 178
PV5594/PV5595
Eu-anti-GST
PV5593
Tracer 178
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV5132 Fl-p53
PV3506 Fl-PKC
11
www.invitrogen.com
Drug Discovery
Kinase
12
Aliase(s)
10 μg
Cat. No.
1 mg
Cat. No.
Accession number
Length
Expression
Tag
Classification
SelectScreen®
Kinase Profiling
Service
Z’-LYTE® assay
Adapta® assay
Assay kit
Substrate
EEF2K
eEF-2K
PV4559
PV4560
NP_037434
Full
E. coli
N-terminal GST Serine/
threonine
kinase
✓
PV4771
Ser/Thr 24
Peptide
EGFR
ErbB1
PV3872
PV4190
NP_005219.2
Cytoplasmic
(668–1210)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3193
Tyr 4 Peptide
EGFR L858R
ErbB1 L858R
PV4128
PV4289
NP_005219.2, L858R Cytoplasmic
(668–1210;
L858R)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3193
Tyr 4 Peptide
EGFR L861Q
ErbB1 L861Q
PV3873
PV4191
NP_005219.2, L861Q Cytoplasmic
(668–1210;
L861Q)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3193
Tyr 4 Peptide
EGFR T790M
ErbB1 T790M
PV4803
PV4804
NP_005219.2
Cytoplasmic
(669–1210;
T790M)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3193
Tyr 4 Peptide
EGFR T790M
L858R
ErbB1 T790M L858R PV4879
PV4880
NP_005219.2
Cytoplasmic
(668–1210;
T790M L858R)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3193
Tyr 4 Peptide
EIF2AK1
HRI
PV5856
PV5857
NP_05528.2
Full
Insect
N-terminal GST Serine/
threonine
kinase
EIF2AK2
MGC126524, PKR,
PRKR
PV4821
PV4822
NP_002750.1
Catalytic
(252–551)
Insect
N-terminal GST Serine/
threonine
kinase
EIF2AK3
PERK
PV5106
PV5107
NP_004827.3
Catalytic
(536–1116)
Insect
N-terminal GST Serine/
threonine
kinase
EPHA1
EPH
PV3841
PV4093
NP_005223.2
Cytoplasmic
(568–976)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3191
Tyr 2 Peptide
EPHA2
ECK
PV3688
PV4094
NP_004422.2
Cytoplasmic
(560–976)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3190
Tyr 1 Peptide
EPHA3
HEK, HEK4
PV3359
PV4095
NP_005224
Cytoplasmic
(569–983)
Insect
C-terminal His
Receptor
tyrosine kinase
✓
PV3190
Tyr 1 Peptide
EPHA4
HEK8
PV3651
PV4096
NP_004429.1
Cytoplasmic
(616–887)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3191
Tyr 2 Peptide
EPHA5
CEK7, EHK1, HEK7,
TYRO4
PV3840
PV4097
NP_004430.1
Cytoplasmic
(595–1037)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3190
Tyr 1 Peptide
EPHA7
HEK11, EHK3
PV3689
PV4098
NP_004431.1
Cytoplasmic
(579–998)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
EPHA8
HEK3, EEK
PV3844
PV4099
NP_065387
Cytoplasmic
(565–1005)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3191
Tyr 2 Peptide
EPHB1
HEK6
PV3786
PV4100
NP_004432
Cytoplasmic
(613–881)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3191
Tyr 2 Peptide
EPHB2
HEK5
PV3625
PV4101
NP_004433
Cytoplasmic
(616–884)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3191
Tyr 2 Peptide
EPHB3
HEK2, TYRO6
PV3658
PV4102
NP_004434
Cytoplasmic
(623–907)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3191
Tyr 2 Peptide
EPHB4
TYRO11
PV3251
PV4103
NP_004435
Cytoplasmic
(561–987)
Insect
C-terminal His
Receptor
tyrosine kinase
✓
PV3190
Tyr 1 Peptide
ERBB2
HER2, NEU
PV3366
PV3590
NP_004439
Cytoplasmic
(676–1255)
Insect
C-terminal His
Receptor
tyrosine kinase
✓
PV4122
Tyr 6 Peptide
ERBB4
HER4
PV3626
PV4104
NP_005226
Cytoplasmic
(708–993)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3190
Tyr 1 Peptide
FER
TYK3
PV3806
PV4076
NP_005237
Catalytic
(541–822)
Insect
N-terminal GST Cytoplasmic
tyrosine kinase
✓
PV3676
Tyr 5 Peptide
FES
Fps
PV3354
PV4077
NP_001996
Full
Insect
C-terminal His
Cytoplasmic
tyrosine kinase
✓
PV3190
Tyr 1 Peptide
FGFR1
H2, H3, H4
PV3146
PV4105
NP_000595
Cytoplasmic
(308–731)
Insect
C-terminal His
Receptor
tyrosine kinase
✓
PV3193
Tyr 4 Peptide
LanthaScreen® Tb activity assay
Antibody
Substrate
LanthaScreen® Eu activity assay
Antibody
Substrate
LanthaScreen® Eu Kinase Binding
Assay
Antibody
Omnia® assay kit
Tracer
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5594/PV5595
Eu-anti-GST
PV5830
Tracer 199
KPZ3121
Y12 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5594/PV5595
Eu-anti-GST
PV5830
Tracer 199
KNZ3101
Y10 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5594/PV5595
Eu-anti-GST
PV5830
Tracer 199
KPZ3121
Y12 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KPZ3121
Y12 Peptide
PV5594/PV5595
Eu-anti-GST
PV5830
Tracer 199
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV4810/PV4811
Tb-peIF2a
PV4809 GFP-eIF2a
PV4810/PV4811
Tb-peIF2 alpha
PV4809
GFP-eIF2a
PV4810/PV4811
Tb-peIF2 alpha
PV4809
GFP-eIF2a
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ3061
Y6 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ3051
Y5 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KPZ3121
Y12 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ3061
Y6 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ3061
Y6 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5594/PV5595
Eu-anti-GST
PV5593
Tracer 178
KNZ3061
Y6 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5593
Tracer 178
KNZ3051
Y5 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5594/PV5595
Eu-anti-GST
PV5593
Tracer 178
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5596/PV5597
Eu-anti-His
PV5592
Tracer 236
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5596/PV5597
Eu-anti-His
PV5592
Tracer 236
SelectScreen®
Cell-Based
Pathway Profiling
Service
✓
CellSensor®
cell line
LanthaScreen® cellular assay
Cell line
K1661 AP-1
ME-180, K1650
c-Fos ME-180
K1587
ERK2 U2OS
Antibody
PV5269/PV5270
ERK2 [pThr185/
pTyr187]
Inquire
KPZ3121
Y12 Peptide
KNZ3051
Y5 Peptide
KPZ3121
Y12 Peptide
13
www.invitrogen.com
Drug Discovery
Kinase
14
Aliase(s)
10 μg
Cat. No.
1 mg
Cat. No.
Accession number
Length
Expression
Tag
Classification
SelectScreen®
Kinase Profiling
Service
Z’-LYTE® assay
Adapta® assay
Assay kit
Substrate
FGFR2
CEK3, TY14, BEK
PV3368
PV4106
NP_075261
Cytoplasmic
(286–705)
Insect
C-terminal His
Receptor
tyrosine kinase
✓
PV3193
Tyr 4 Peptide
FGFR3
CEK2, JTK4
PV3145
PV4107
NP_000133
Cytoplasmic
(399–806)
Insect
N-terminal His
Receptor
tyrosine kinase
✓
PV3193
Tyr 4 Peptide
FGFR3 K650E
ACH, CEK2
PV4392
PV4428
NP_000133, K650E
Cytoplasmic
(398–806;
K650E)
Insect
N-terminal His
Receptor
tyrosine kinase
✓
PV3193
Tyr 4 Peptide
FGFR4
JTK2
P3054
PR4380A
NP_002002
Cytoplasmic
(460–802)
Insect
N-terminal His
Receptor
tyrosine kinase
✓
PV3193
Tyr 4 Peptide
FGR
c-fgr, p55c-fgr, SRC2 P3041
PV4078
NP_005239
Full
Insect
C-terminal His
Cytoplasmic
tyrosine kinase
✓
PV3191
Tyr 2 Peptide
FLT1
VEGFR1, FLT
PV3666
PR6731A
NP_002010
Cytoplasmic
(781–1338)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3193
Tyr 4 Peptide
FLT3
FLK2, STK1, CD135
PV3182
PV4108
NP_004110
Cytoplasmic
(564–958)
Insect
C-terminal His
Receptor
tyrosine kinase
✓
PV3191
Tyr 2 Peptide
FLT3 D835Y
FLK2, STK1, CD135
PV3967
PV4271
NP_004110, D835Y
Cytoplasmic
(564–958;
D835Y)
Insect
C-terminal His
Receptor
tyrosine kinase
✓
PV3191
Tyr 2 Peptide
FLT4
VEGFR3, PCL
PV4129
PV4290
NP_891555
Cytoplasmic
(800–1297;
Q890H V1128L
H1146R)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3193
Tyr 4 Peptide
FRAP1
mTOR, FRAP, FRAP2, PV4753
TOR, RAFT1
PV4754
NP_004949.1
Catalytic
(1360–2549)
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3670
Ser/Thr 11
Peptide
FRK
PTK5, GTK, RAK
PV3874
PV4192
NP_002022
Full
Insect
N-terminal GST Cytoplasmic
tyrosine kinase
✓
PV3190
Tyr 1 Peptide
FYN
SLK, SYN, FynT
P3042
PR4163A
NP_694592
Full
Insect
C-terminal His
✓
PV3191
Tyr 2 Peptide
GRK4
GPRK4, GPRK2L,
GRK4 alpha, IT11
PV3807
PV3999
NP_892027
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3802
Ser/Thr 16
Peptide
GRK5
GPRK5
PV3824
PV4000
NP_005299
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3802
Ser/Thr 16
Peptide
GRK6
GPRK6
PV3661
PV4001
NP_001004106
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3802
Ser/Thr 16
Peptide
GRK7
GPRK7
PV3823
PV4125
NP_631948
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3802
Ser/Thr 16
Peptide
GSG2
haspin
PV5708
PV5709
NP_114171.1
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
GSK3A
GSK3 alpha
PV3270
PV3596
NP_063937
Full
Insect
C-terminal His
Serine/
threonine
kinase
✓
PV3324
Ser/Thr 9
Peptide
GSK3B
GSK3 beta
PV3365
PV3597
NP_002084
Full
Insect
C-terminal His
Serine/
threonine
kinase
✓
PV3324
Ser/Thr 9
Peptide
HCK
JTK9
P2908
PV4079
NP_002101
Full
Insect
C-terminal His
Cytoplasmic
tyrosine kinase
✓
PV3191
Tyr 2 Peptide
HIPK1
Myak, Nbak2,
KIAA0630
PV4561
PV4562
NP_689909
Catalytic
(158–555)
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3324
Ser/Thr 9
Peptide
Cytoplasmic
tyrosine kinase
PV5707
Histone H3 (1-20)
Peptide
LanthaScreen® Tb activity assay
Antibody
Substrate
LanthaScreen® Eu activity assay
Antibody
Substrate
LanthaScreen® Eu Kinase Binding
Assay
Antibody
Omnia® assay kit
Tracer
SelectScreen®
Cell-Based
Pathway Profiling
Service
CellSensor®
cell line
Cell line
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
KNZ3111
Y11 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
KNZ3111
Y11 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
KPZ3121
Y12 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5596/PV5597
Eu-anti-His
PV5592
Tracer 236
KNZ3051
Y5 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ3081
Y8 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5596/PV5597
Eu-anti-His
PV5592
Tracer 236
KNZ3051
Y5 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5596/PV5597
Eu-anti-His
PV5592
Tracer 236
KNZ3051
Y5 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ3081
Y8 Peptide
PV4757/PV4758
Tb-4E-BP1
PV4759
GFP-4E-BP1
PV5594/PV5595
Eu-anti-GST
PV6087
Tracer 314
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ3051
Y5 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5596/PV5597
Eu-anti-His
PV5592
Tracer 236
KNZ3051
Y5 Peptide
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV3562/PV3563
PV3507 Fl-IKK
Tb-I kappa B alpha
pSer32
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV3562/PV3563
PV3507 Fl-IKK
Tb-I kappa B alpha
pSer32
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV5596/PV5597
Eu-anti-His
PV5592
Tracer 236
KNZ1131
ST13 Peptide
PV5596/PV5597
Eu-anti-His
PV5592
Tracer 236
KNZ1131
ST13 Peptide
✓
K1676
LEF/TCF HCT116
PV5596/PV5597
Eu-anti-His
PV5592
Tracer 236
KNZ3051
Y5 Peptide
✓
K1671
NFAT Jurkat
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
LanthaScreen® cellular assay
Antibody
KNZ3101
Y10 Peptide
K1528 PRAS40
HEK293E, K1593
PDCD4 HEK293E,
K1615 AKT
HEK293E
PV5580/PV5581
PRAS40 [pSer183],
PV5104/PV5105
PDCD4 [pSer457],
PV5267/PV5268
AKT [pSer473]
K1490
NFAT RA-1
15
www.invitrogen.com
Drug Discovery
Kinase
Aliase(s)
HIPK2
1 mg
Cat. No.
Accession number
Length
Expression
Tag
Classification
SelectScreen®
Kinase Profiling
Service
PV5275
PV5276
NP_073577.3
Catalytic
(165–564)
Insect
N-terminal GST Serine/
threonine
kinase
✓
Z’-LYTE® assay
Adapta® assay
Assay kit
Substrate
PV3324
Ser/Thr 9
Peptide
HIPK3
DYRK6, Pky, YAK1
PV4209
PV4324
NP_005725.2
Catalytic
(163–562)
Insect
N-terminal His
Serine/
threonine
kinase
✓
HIPK4
FLK32818
PV3852
PV4176
NP_653286
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV4319
Ser/Thr 18
Peptide
IGF1R
JTK13, CD221
PV3250
PV3592
NP_000866
Cytoplasmic
(960–1397)
Insect
C-terminal His
✓
PV3190
Tyr 1 Peptide
IKBKB
IKK beta, NFKBIKB,
IKK2
PV3836
PR7489A
NP_001547
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3178
Ser/Thr 5
Peptide
IKBKE
IKK epsilon, IKKE,
IKKI
PV4875
PV4876
NP_054721
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3670
Ser/Thr 11
Peptide
PV3781
PV4110
NP_000199
Cytoplasmic
(1011–1382)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3190
Tyr 1 Peptide
PV3193
Tyr 4 Peptide
INSR
16
10 μg
Cat. No.
Receptor
tyrosine kinase
INSRR
IRR
PV3808
PV4111
NP_055030
Cytoplasmic
(944–1297)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
IRAK1
IRAK, pelle
PV4403
PV4439
NP_001560
Catalytic
(194–712)
Insect
N-terminal GST Serine/
threonine
kinase
✓
IRAK4
LOC51135,
NY-REN-64
PV3362
PV4002
NP_057207,
AAH13316
Full
Insect
N-terminal His
✓
PV3180
Ser/Thr 7
Peptide
ITK
EMT, LYK, PSCTK2
PV3875
PV4193
NP_005537
Full
Insect
N-terminal GST Cytoplasmic
tyrosine kinase
✓
PV3190
Tyr 1 Peptide
JAK1
JAK1A, JAK1B
PV4774
PV4775
NP_002218.2
Catalytic
(866–1154)
Insect
N-terminal GST Cytoplasmic
tyrosine kinase
✓
PV4122
Tyr 6 Peptide
JAK2
PV4210
PV4288
NP_004963
Catalytic
(809–1132 +9)
Insect
N-terminal GST Cytoplasmic
tyrosine kinase
✓
PV4122
Tyr 6 Peptide
JAK2 JH1 JH2
PV4393
PV4429
NP_004963
Catalytic
(532–1132)
Insect
N-terminal GST Cytoplasmic
tyrosine kinase
✓
PV4122
Tyr 6 Peptide
JAK2 JH1 JH2
V617F
PV4336
PV4347
NP_004963
Catalytic
(532–1132;
V617F)
Insect
N-terminal GST Cytoplasmic
tyrosine kinase
✓
PV4122
Tyr 6 Peptide
Serine/
threonine
kinase
JAK3
LJAK, JAKL
PV3855
PV4080
NP_000206
Catalytic
(781–1124)
Insect
N-terminal GST Cytoplasmic
tyrosine kinase
✓
PV4122
Tyr 6 Peptide
KDR
VEGFR2, VEGFR,
FLK1
PV3660
PR5992A
NP_002244
Cytoplasmic
(789–1356)
Insect
C-terminal His
✓
PV3190
Tyr 1 Peptide
Receptor
tyrosine kinase
LanthaScreen® Tb activity assay
Antibody
Substrate
LanthaScreen® Eu activity assay
Antibody
Substrate
LanthaScreen® Eu Kinase Binding
Assay
Antibody
Omnia® assay kit
Tracer
SelectScreen®
Cell-Based
Pathway Profiling
Service
LanthaScreen® cellular assay
CellSensor®
cell line
✓
K1661
AP-1 ME-180
Cell line
Antibody
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
KNZ3041
Y4 Peptide
✓
K1468
T-REx™ FOXO3DBE HeLa
K1834 IGFR1
GripTite™, K1528
PRAS40 HEK293E,
K1593 PDCD4
HEK293E, K1615
AKT HEK293E
PV5580/PV5581
PRAS40 [pSer183],
PV5104/PV5105
PDCD4 [pSer457],
PV5267/PV5268
AKT [pSer473],
PV5584/PV5585
AKT [pThr308]
PV3562/PV3563
PV3507 Fl-IKK
Tb-I kappa B alpha
pSer32
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1201
ST20 Peptide
✓
K1667
NFkB ME-180
K1681 IkB
GripTite™
PV3562/PV3563
IKBa pSer32
PV3562/PV3563
PV3507 Fl-IKK
Tb-I kappa B alpha
pSer32
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1241
ST24 Peptide
✓
K1655
ISRE HEK
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ3041
Y4 Peptide
✓
K1468
T-REx™ FOXO3DBE HeLa
K1528 PRAS40
HEK293E, K1593
PDCD4 HEK293E,
K1615 AKT
HEK293E
PV5580/PV5581
PRAS40 [pSer183],
PV5104/PV5105
PDCD4 [pSer457],
PV5267/PV5268
AKT [pSer473],
PV5584/PV5585
AKT [pThr308]
✓
K1662
NFkB THP-1
✓
K1662
NFkB THP-1
✓
K1671
NFAT Jurkat
✓
K1652 SIE THP1,
K1488 STAT3
K1649 SIE HEK,
GripTite™, K1469
K1648 SIE ME-180, STAT1 U2OS
K1651 GAS
ME-180, K1653 irf1
CTLL-2, K1655 ISRE
HEK, K1656 ISRE
Jurkat
PV4846/PV4847
STAT3 [pTyr705],
PV4844/PV4845
STAT1 [pTyr701]
✓
K1657 irf1 TF-1,
K1598 STAT5 TF1
K1652 SIE THP1,
K1649 SIE HEK,
K1648 SIE ME-180,
K1641 GAS
ME-180, K1653 irf1
CTLL-2, K1655 ISRE
HEK, K1656 ISRE
Jurkat
PV5266/PV5267
STAT5 A/B
[pTyr694/699]
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
KNZ3041
Y4 Peptide
PV5594/PV5595
Eu-anti-GST
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV4844/PV4845
Tb-STAT1
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5592
Tracer 236
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ3061
Y6 Peptide
PV5211
GFP-STAT1
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV4844/PV4845
Tb-STAT1
PV5211
GFP-STAT1
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ3111
Y11 Peptide
PV4844/PV4845
Tb-STAT1
PV5211
GFP-STAT1
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KPZ3121
Y12 Peptide
PV4844/PV4845
Tb-STAT1
PV5211
GFP-STAT1
PV4844/PV4845
Tb-STAT1
PV5211
GFP-STAT1
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
KPZ3121
Y12 Peptide
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ3071
Y7 Peptide
PV5596/PV5597
Eu-anti-His
PV5592
Tracer 236
KNZ3081
Y8 Peptide
K1647 irf1 HEL
✓
K1588 STAT5 U2OS PV5266/PV5267
(JAK2V617F)
STAT5 A/B
[pTyr694/699]
K1653 irf1 CTLL-2,
K1646 STAT6 RA-1
17
www.invitrogen.com
Drug Discovery
Kinase
Aliase(s)
1 mg
Cat. No.
Accession number
Length
Expression
Tag
Classification
SelectScreen®
Kinase Profiling
Service
Z’-LYTE® assay
Adapta® assay
Assay kit
Substrate
KIT
cKit, PBT, SCFR,
CD117
P3081
PV3589
NP_000213
Cytoplasmic
(544–976)
Insect
N-terminal His
Receptor
tyrosine kinase
✓
PV4122
Tyr 6 Peptide
KIT T670I
cKit, PBT, SCFR,
CD117
PV3869
PV4187
NP_000213, T670I
Cytoplasmic
(544–976;
T670I)
Insect
N-terminal His
Receptor
tyrosine kinase
✓
PV4122
Tyr 6 Peptide
KIT V654A
cKit, PBT, SCFR,
CD117
PV4132
PV4291
NP_000213, V654A
Cytoplasmic
(544–976;
V654A)
Insect
N-terminal His
Receptor
tyrosine kinase
✓
P3043
PV4081
NP_005347
Full
Insect
C-terminal His
Cytoplasmic
tyrosine kinase
✓
PV4337
PV4348
NP_002305
Catalytic
(285–638)
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV3860
PV4349
NP_005560
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
AURA17, PARK8,
RIPK7, ROCO2
PV4873
PV4874
NP_940980.2
Catalytic
(970–2527)
Insect
N-terminal GST Serine/
threonine
kinase
✓
LRRK2 D1994A AURA17, PARK8,
RIPK7, ROCO2
PV6051
PV6052
NP_940980.2
(D1994A)
Catalytic
(970–2527)
Insect
N-terminal GST Serine/
threonine
kinase
LRRK2 G2019S
AURA17, PARK8,
RIPK7, ROCO2
PV4881
PV4882
NP_940980.2
(G2019S)
Catalytic
(970–2527)
Insect
N-terminal GST Serine/
threonine
kinase
LRRK2 I2020T
AURA17, PARK8,
RIPK7, ROCO2
PV5854
PV5855
NP_940980.2
(I2020T)
Catalytic
(970–2527)
Insect
N-terminal GST Serine/
threonine
kinase
PV5093 ERM
(LRRKtide)
LRRK2 R1441C
AURA17, PARK8,
RIPK7, ROCO2
PV5858
PV5859
NP_940980.2
(R1441C)
Catalytic
(970–2527)
Insect
N-terminal GST Serine/
threonine
kinase
PV5093 ERM
(LRRKtide)
LTK
TYK1
PV4651
PV4652
NP_002335.2
Cytoplasmic
(450–864)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV4122
Tyr 6 Peptide
LYNA
P2906
PV4082
NP_002341
Full
Insect
C-terminal His
Cytoplasmic
tyrosine kinase
✓
PV3191
Tyr 2 Peptide
LYNB
P2907
PV4083
AAH59394
Full
Insect
C-terminal His
Cytoplasmic
tyrosine kinase
✓
PV3191
Tyr 2 Peptide
PV3176
Ser/Thr 3
Peptide
LCK
LIMK1
LIMK
LIMK2
LRRK2
18
10 μg
Cat. No.
PV5093 ERM
(LRRKtide)
PV5093 ERM
(LRRKtide)
✓
MAP2K1
MEK1, MAPKK1
PV3303
PV4003
NP_002746
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
MAP2K1,
mutant
MEK1, MAPKK1
P3099
PV4004
NP_002746, S218D,
S222D
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
MAP2K2
MEK2, MKK2
PV3615
PV4005
NP_109587
Full
Insect
C-terminal His
Serine/
threonine
kinase
✓
MAP2K3
MEK3, MKK3
PV3662
PV4006
NP_002747
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
MAP2K6
MKK6, MEK6
PV3318
PV4007
NP_002749
Full
Insect
N-terminal His
✓
Serine/
threonine
kinase
PV3191
Tyr 2 Peptide
PV5093 ERM
(LRRKtide)
PV3176
Ser/Thr 3
Peptide
PV3176
Ser/Thr 3
Peptide
LanthaScreen® Tb activity assay
Antibody
Substrate
LanthaScreen® Eu activity assay
Antibody
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
Substrate
LanthaScreen® Eu Kinase Binding
Assay
Antibody
Omnia® assay kit
Tracer
PV5693/PV5863
AF647-GT
SelectScreen®
Cell-Based
Pathway Profiling
Service
CellSensor®
cell line
LanthaScreen® cellular assay
Cell line
Antibody
KPZ3091
Y9 Peptide
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
PV5693/PV5863
AF647-GT
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5593
Tracer 178
PV5693/PV5863
AF647-GT
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KPZ3121
Y12 Peptide
KNZ3051
Y5 Peptide
PV4899/PV4900
Tb-pERM
(pLRRKtide)
PV4901 Fl-ERM
(LRRKtide)
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV4899/
PV4900 Tb-ERM
(pLRRKtide)
PV4901 Fl-ERM
(LRRKtide)
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV4899/PV4900
Tb-pERM
(pLRRKtide)
PV4901 Fl-ERM
(LRRKtide)
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV4899/
PV4900 Tb-ERM
(pLRRKtide)
PV4901 Fl-ERM
(LRRKtide)
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV4899/
PV4900 Tb-ERM
(pLRRKtide)
PV4901 Fl-ERM
(LRRKtide)
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
KNZ3051
Y5 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
KNZ3051
Y5 Peptide
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
✓
K1671
NFAT Jurkat
K1490
NFAT RA-1
✓
K1661 AP-1
ME-180, K1650
c-fos ME-180
✓
K1661 AP-1
ME-180, K1650
c-fos ME-180
✓
K1661 AP-1
ME-180
K1587 ERK2 U2OS
PV5269/PV5270
ERK2 [pThr185/
pTyr187]
19
www.invitrogen.com
Drug Discovery
Kinase
20
Aliase(s)
10 μg
Cat. No.
1 mg
Cat. No.
Accession number
Length
Expression
Tag
Classification
SelectScreen®
Kinase Profiling
Service
Z’-LYTE® assay
Adapta® assay
Assay kit
Substrate
MAP2K6,
mutant
MKK6, MEK6
PV3293
PV4008
NP_002749,
S207E, T211E
Full
E. coli
N-terminal His
Serine/
threonine
kinase
✓
MAP3K2
MEKK2, MEKK2B
PV3822
PV4010
AAF63496.3
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
MAP3K3
MEKK3, MAPKKK3
PV3876
PV4194
NP_002392
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
MAP3K5
ASK1, MAPKKK5
PV3809
PV4011
NP_005914
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
MAP3K7/
MAP3K7IP1
TAK1/TAB1
PV4394
PV4430
NP_663306,
NP_006107
Full/catalytic
(437–504)
Insect
N-terminal His
Serine/
threonine
kinase
✓
MAP3K8
COT1, EST, ESTF
PV4313
PV4330
NP_005195
Catalytic (30–
397)
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3176
Ser/Thr 3
Peptide
MAP3K9
MLK1, PRKE1
PV3787
PV4012
NP_149132
Catalytic
(1–500)
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
MAP3K10
MLK2, MST
PV3877
PV4195
NP_002437
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
MAP3K11
MLK3, SPRK
PV3788
PV4009
NP_002410.1
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
MAP3K14
NIK
PV4902
PV4903
NP_003945.2
Catalytic
(318–947)
Insect
N-terminal GST Serine/
threonine
kinase
✓
MAP4K2
GCK, BL44
PV4211
PV4281
NP_004570.2
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
MAP4K4
HGK, NIK
PV3687
PV4013
NP_004825
Catalytic
(1–328)
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
MAP4K5
KHS, GCKR
PV3682
PV4014
NP_942089
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
MAPK1
ERK2, ERK
PV3313
PV3595
NP_620407
Full
E. coli
N-terminal GST Serine/
threonine
kinase
✓
PV3176
Ser/Thr 3
Peptide
MAPK3
ERK1, P44ERK1,
PRKM3
PV3311
PV3594
NP_002737
Full
E. coli
N-terminal GST Serine/
threonine
kinase
✓
PV3176
Ser/Thr 3
Peptide
MAPK8
JNK1, JNK, SAPK1
PV3319
PV3593
NP_002741.1
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV3177
Ser/Thr 4
Peptide
MAPK9
JNK2
PV3620
PV4018
NP_002743
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV3177
Ser/Thr 4
Peptide
MAPK10
JNK3
PV4563
PV4564
NP_002744
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3177
Ser/Thr 4
Peptide
MAPK11
p38 beta, P38b,
P382, SAPK2
PV3679
PR7171A
NP_002742
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV3799
Ser/Thr 15
Peptide
MAPK12
p38 gamma, ERK6,
SAPK3
PV3654
PV4016
NP_002960
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV3176
Ser/Thr 3
Peptide
LanthaScreen® Tb activity assay
Antibody
Substrate
LanthaScreen® Eu activity assay
Antibody
Substrate
LanthaScreen® Eu Kinase Binding
Assay
Antibody
Omnia® assay kit
Tracer
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
PV4813/PV4814
Tb-pMAP2K1
PV4812
Fl-MAP2K1
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV4813/PV4814
Tb-pMAP2K1
PV4812
Fl-MAP2K1
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV4813/PV4814
Tb-pMAP2K1
PV4812
Fl-MAP2K1
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV4813/PV4814
Tb-pMAP2K1
PV4812
Fl-MAP2K1
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
PV4813/PV4814
Tb-pMAP2K1
PV4812
Fl-MAP2K1
PV4813/PV4814
Tb-pMAP2K1
PV4812
Fl-MAP2K1
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1081
ST8 Peptide
PV4813/PV4814
Tb-pMAP2K1
PV4812
Fl-MAP2K1
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1061
ST6 Peptide
PV4813/PV4814
Tb-pMAP2K1
PV4812
Fl-MAP2K1
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1081
ST8 Peptide
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
SelectScreen®
Cell-Based
Pathway Profiling
Service
CellSensor®
cell line
LanthaScreen® cellular assay
Cell line
✓
K1661
AP-1 ME-180
✓
K1661
AP-1 ME-180
✓
K1667
NFkB ME-180
PV3560/PV3561
Tb-pSer (pPKC)
PV3506 Fl-PKC
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
PV3560/PV3561
Tb-pSer (pPKC)
PV3506 Fl-PKC
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1321
ST32 Peptide
PV3560/PV3561
Tb-pSer (pPKC)
PV3506 Fl-PKC
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KPZ1261
ST26 Peptide
PV4451/PV4452
Tb-pATF2
PV4445 Fl-GFPATF2
KNZ1171
ST17 Peptide
✓
K1661 AP-1
ME-180
PV4451/PV4452
Tb-pATF2
PV4445
Fl-GFP-ATF2
KNZ1171
ST17 Peptide
✓
K1661 AP-1
ME-180
PV4451/PV4452
Tb-pATF2,
PV4453/PV4454
Tb-pc- Jun
PV4445 Fl-GFPATF2, PV4446
GFP-c-Jun
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5830
Tracer 199
KNZ1101
ST10 Peptide
✓
K1661 AP-1
ME-180, K1658
AP-1 HEK
PV4451/PV4452
Tb-pATF2,
PV4453/PV4454
Tb-pc- Jun
PV4445 Fl-GFPATF2, PV4446
GFP-c-Jun
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5830
Tracer 199
PV4451/PV4452
Tb-pATF2
PV4453/PV4454
Tb-pc- Jun
PV4445 Fl-GFPATF2 PV4446
GFP-c-Jun
PV5594/PV5595
Eu-anti-GST
PV5830
Tracer 199
PV4451/PV4452
Tb-pATF2
PV4445 GFP-ATF2
KNZ1091
ST9 Peptide
PV4451/PV4452
Tb-pATF2
PV4445 GFP-ATF2
KNZ1071
ST7 Peptide
Antibody
K1680 c-Jun (1-79)
HeLa, K1679 ATF2
(19-106) A549
PV4453 c-Jun
(pSer73), PV4451
ATF2 (pThr71)
K1679 ATF2 (19106) A549
PV4451/PV4452
ATF2 (pSer71)
KNZ1101
ST10 Peptide
21
www.invitrogen.com
Drug Discovery
Kinase
Aliase(s)
1 mg
Cat. No.
Accession number
Length
Expression
Tag
Classification
SelectScreen®
Kinase Profiling
Service
Z’-LYTE® assay
Adapta® assay
Assay kit
Substrate
MAPK13
p38 delta, SAPK4
PV3656
PV4017
NP_002745
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV3176
Ser/Thr 3
Peptide
MAPK14
p38 alpha, p38,
Mxi2, SAPK2A
PV3304
PV3598
NP_620581
Full
E. coli
N-terminal GST Serine/
threonine
kinase
✓
PV3177
Ser/Thr 4
Peptide
MAPKAPK2
MK2
PV3317
PV4019
NP_116584
Full
E. coli
N-terminal His
Serine/
threonine
kinase
✓
PV3177
Ser/Thr 4
Peptide
MAPKAPK3
3PK, MAPKAP3
PV3299
PV4020
NP_004626
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV3177
Ser/Thr 4
Peptide
MAPKAPK5
PRAK
PV3301
PV4021
NP_003659
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV3177
Ser/Thr 4
Peptide
MARK1
MARK, KIAA1477_
GMC126513
PV4395
PV4431
NP_061120, V16E,
T20S
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV4535
Ser/Thr 21
Peptide
MARK2
EMK1, PAR-1.
MGC99619
PV3878
PV4196
NP_059672.2
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV4535
Ser/Thr 21
Peptide
PV4819
PV4820
NP_002367.3
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV5116
Ser/Thr 25
Peptide
MARK3
MARK4
KIAA1860
PV3851
PV4175
NP_113605
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV5116
Ser/Thr 25
Peptide
MATK
Hyl, CTK
PV3370
PV4084
NP_647611
Full
E. coli
C-terminal His
✓
PV3190
Tyr 1 Peptide
MELK
RP11-8N6.1, HPK38,
KIAA0175
PV4823
PV4824
NP_055606.1
Catalytic
(1–340)
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV4316
Ser/Thr 17
Peptide
MERTK
cMER. MER
PV3627
PV4112
NP_006334
Cytoplasmic
(578–872)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3191
Tyr 2 Peptide
MET
cMet, HGFR, RCCP2
PV3143
PV3588
NP_000236.2
Cytoplasmic
(956–1390)
Insect
N-terminal His
Receptor
tyrosine kinase
✓
PV4122
Tyr 6 Peptide
MET M1250T
cMet, HGFR, RCCP2
PV3968
PV4272
NP_000236.2
Cytoplasmic
(956–1390;
M1250T)
Insect
N-terminal His
Receptor
tyrosine kinase
✓
PV4122
Tyr 6 Peptide
MINK1
MINK, B55, ZC3
PV3810
PV4022
NP_056531
Catalytic
(1–320)
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
MKNK1
MNK1
PV6023
PV6024
NP_945324
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
MKNK2
MNK2
PV5607
PV5608
NP_060042.2
Full
Insect
N-terminal GST Serine/
threonine
kinase
MLCK
MLCK2
PV3835
PV4023
NP_872299, R175G
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
MST1R
RON, CDw136
PV4314
PV4332
NP_002438
Cytoplasmic
(983–1400)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV4122
Tyr 6 Peptide
MST4
PV3690
PV4024
NP_057626.2
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
MUSK
PV3834
PV4113
NP_005583.1
Cytoplasmic
(519–869)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3193
Tyr 4 Peptide
PV4339
PV4350
NP_444253
Catalytic
(1428–1771)
Insect
N-terminal GST Serine/
threonine
kinase
✓
MYLK
22
10 μg
Cat. No.
MLCK
Cytoplasmic
tyrosine kinase
RS Peptide
(inquire)
LanthaScreen® Tb activity assay
Antibody
Substrate
PV4451/PV4452
Tb-pATF2
PV4445
GFP-ATF2
PV4451/PV4452
Tb-pATF2
PV4445
GFP-ATF2
LanthaScreen® Eu activity assay
Antibody
Substrate
LanthaScreen® Eu Kinase Binding
Assay
Antibody
Omnia® assay kit
Tracer
SelectScreen®
Cell-Based
Pathway Profiling
Service
LanthaScreen® cellular assay
CellSensor®
cell line
Cell line
Antibody
KPZ1291
ST29 Peptide
PV5594/PV5595
Eu-anti-GST
✓
PV5830
Tracer 199
PV3566 Tb-pCREB PV3508
pSer133
Fl-CREBtide
KNZ1031
ST3 Peptide
PV3566 Tb-pCREB PV3508
pSer133
Fl-CREBtide
KNZ1031
ST3 Peptide
K1662 NFkB-bla
THP1, K1659 c-fos
HEK, K1650 c-fos
ME-180
K1679 ATF2 (19106) A549
PV4451/PV4452
ATF2 (pSer71)
K1587 ERK2 U2OS
PV5269/PV5270
ERK2 [pThr185/
pTyr187]
K1587 ERK2 U2OS
PV5269/PV5270
ERK2 [pThr185/
pTyr187]
KNZ1231
ST23 Peptide
PV3560/PV3561
Tb-pSer (pPKC)
PV3506 Fl-PKC
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KPZ1311
ST31 Peptide
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KPZ1311
ST31 Peptide
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV5693/PV5863
AF647-GT
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
V5693/PV5863
AF647-GT
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ3061
Y6 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
V5693/PV5863
AF647-GT
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
KPZ3121
Y12 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV3560/PV3561
Tb-pSer (pPKC)
PV3506 Fl-PKC
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV3560/PV3561
Tb-pSer (pPKC)
PV3506 Fl-PKC
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
✓
K1661 AP-1
ME-180
✓
K1661 AP-1
ME-180
KNZ3091
Y9 Peptide
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1081
ST8 Peptide
KNZ3081
Y8 Peptide
23
www.invitrogen.com
Drug Discovery
Kinase
Aliase(s)
1 mg
Cat. No.
Accession number
Length
Expression
Tag
Classification
SelectScreen®
Kinase Profiling
Service
Z’-LYTE® assay
Adapta® assay
Assay kit
Substrate
MYLK2
skMLCK, KMLC,
MLCK
PV3757
PV4025
NP_149109
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3793
Ser/Thr 13
Peptide
NEK1
KIAA1901,
NY-REN-55
PV4202
PV4282
NP_036356
Catalytic
(1–505)
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
NEK11
FLJ23495
PV4647
PV4648
NP_079076
Full
Insect
N-terminal GST Serine/
threonine
kinase
NEK2
NLK1, HsPK21
PV3360
PV4026
NP_002488
Full
Insect
C-terminal His
✓
PV3180
Ser/Thr 7
Peptide
PV3821
PV4027
NP_689933
Full
Insect
N-terminal GST Serine/
threonine
kinase
NEK3
Serine/
threonine
kinase
NEK4
NRK2, STK2
PV4315
PV4331
NP_003148
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV4316
Ser/Thr 17
Peptide
NEK6
SID6-1512
PV3353
PV4028
NP_055212
Catalytic
(8–313)
Insect
C-terminal His
Serine/
threonine
kinase
✓
PV4572
Ser/Thr 22
Peptide
NEK7
PV3833
PV4029
NP_598001.1
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV4572
Ser/Thr 22
Peptide
NEK9
NERC, NERCC, NEK8 PV4653
PV4654
NP_149107.2
Catalytic
(347–732)
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
NLK
FLJ21033,
DKFZp761G1211
PV4309
PV4328
NP_057315.1
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
NTRK1
TRKA, MTK, TRK
PV3144
PV4114
NP_002520
Cytoplasmic
(441–796)
Insect
C-terminal His
Receptor
tyrosine kinase
✓
PV3190
Tyr 1 Peptide
NTRK2
TRKB
PV3616
PR5995A
NP_006171
Cytoplasmic
(526–838)
Insect
C-terminal His
Receptor
tyrosine kinase
✓
PV3190
Tyr 1 Peptide
NTRK3
TRKC
PV3617
PV4116
NP_002521
Cytoplasmic
(510–825)
Insect
C-terminal His
Receptor
tyrosine kinase
✓
PV3190
Tyr 1 Peptide
NUAK1
ARK5
PV4127
PV4283
NP_055655
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
PAK1
PAK alpha
PV3820
PV4030
NP_002567
Full
Insect
N-terminal GST Serine/
threonine
kinase
PAK2
PAK delta, PAK6
PV4565
PV4566
NP_002568.2
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV4532
Ser/Thr 20
Peptide
PAK3
bPAK, hPAK3
PV3789
PV4031
NP_002569
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV4532
Ser/Thr 20
Peptide
PV4212
PV4284
NP_005875
Catalytic
(295–591)
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV4532
Ser/Thr 20
Peptide
PAK4
PV3796
Ser/Thr 14
Peptide
PAK6
PAK5
PV3502
PV4032
NP_064553
Full
Insect
C-terminal His
Serine/
threonine
kinase
✓
PV4532
Ser/Thr 20
Peptide
PAK7
PAK5
PV4405
PV4441
NP_065074
Catalytic
(425–719)
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV4532
Ser/Thr 20
Peptide
PV3972
PV4276
NP_055963
Catalytic
(879–1323)
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
PV3811
PV4117
NP_006197
Cytoplasmic
(550–1089)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3193
Tyr 4 Peptide
PASK
PDGFRA
24
10 μg
Cat. No.
PDGFR alpha,
CD140A, PDGFR2
LanthaScreen® Tb activity assay
Antibody
Substrate
LanthaScreen® Eu activity assay
Antibody
Substrate
LanthaScreen® Eu Kinase Binding
Assay
Antibody
PV5594/PV5595
Eu-anti-GST
PV3560/PV3561
Tb-pSer (pPKC)
Omnia® assay kit
Tracer
SelectScreen®
Cell-Based
Pathway Profiling
Service
LanthaScreen® cellular assay
CellSensor®
cell line
Cell line
Antibody
PV5592
Tracer 236
PV3506 Fl-PKC
KNZ1271
ST27 Peptide
PV5594/PV5595
Eu-anti-GST
✓
K1661
AP-1 ME-180
PV4453/PV4454
Tb-pc-Jun
PV4446
GFP-c-Jun
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
KNZ3081
Y8 Peptide
✓
K1516 TRkA-NFAT
CHO K1
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
KNZ3051
Y5 Peptide
✓
K1491 TRkB-NFAT
CHO K1
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
✓
K1515 TRkC-NFAT
CHO K1
✓
K1468 T-REx™
FOXO3-DBE HeLa
PV5596/PV5597
Eu-anti-His
PV3564/PV3565
Tb-Crosstide
PV5593
Tracer 178
PV5592
Tracer 236
PV3509
Fl-Crosstide
KPZ1311
ST31 Peptide
KNZ1051
ST5 Peptide
KNZ1051
ST5 Peptide
KNZ1051
ST5 Peptide
PV3564/PV3565
Tb-Crosstide
PV3509
Fl-Crosstide
PV3564/PV3565
Tb-Crosstide
PV3509
Fl-Crosstide
PV3566 pCREB
Tb-pSer133
PV3508
Fl-CREBtide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV5596/PV5597
Eu-anti-His
PV5592
Tracer 236
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
KNZ1051
ST5 Peptide
KNZ1051
ST5 Peptide
KNZ1061
ST6 Peptide
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ3091
Y9 Peptide
K1587 ERK2 U2OS
PV5269/PV5270
ERK2 [pThr185/
pTyr187]
25
www.invitrogen.com
Drug Discovery
Kinase
Aliase(s)
1 mg
Cat. No.
Accession number
Length
Expression
Tag
Classification
SelectScreen®
Kinase Profiling
Service
Z’-LYTE® assay
Adapta® assay
Assay kit
Substrate
PDGFRA D842V PDGFR alpha,
CD140A, PDGFR2
PV4203
PV4292
NP_006197, D842V
Cytoplasmic
(668–1210;
D842V)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3193
Tyr 4 Peptide
PDGFRA T674I
PDGFR alpha,
CD140A, PDGFR2
PV3847
PV4118
NP_006197, T674I
Cytoplasmic
(550–1089;
T674I)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3193
Tyr 4 Peptide
PDGFRA V561D PDGFR alpha,
CD140A, PDGFR2
PV4680
PV4681
NP_006197, V561D
Cytoplasmic
(550–1089)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3193
Tyr 4 Peptide
PDGFRB
P3082
PV3591
NP_002600
Cytoplasmic
(558–1106)
Insect
N-terminal His
Receptor
tyrosine kinase
✓
PV3193
Tyr 4 Peptide
P3001
PV4033
NP_002604
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV3179
Ser/Thr 6
Peptide
PV5710
PV5711
NP_002603.1
Full
Insect
N-terminal GST Serine/
threonine
kinase
PHKG1
PV3853
PV4034
NP_006204
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
PHKG2
PV4555
PV4556
NP_000285
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
PDGFR beta, JTK12,
CD140B, PDGFR1
PDK1
PDK4
26
10 μg
Cat. No.
PDHK4
PI4KA
PI4K alpha
PV5689 (20 μg) PV5690
NP_002641.1
Full
Insect
N-terminal GST Lipid kinase
✓
PV5122 PI:PS
PI4KB
PI4K beta
PV5277 (20 μg) PV5278
NP_002642.1
Full
Insect
N-terminal GST Lipid kinase
✓
PV5122 PI:PS,
PV5371 PI
PIK3C2A
PI3K-C2 alpha
PV5586 (20 μg) PV5587
NP_002636.1
Catalytic
(142–1412)
Insect
N-terminal GST Lipid kinase
✓
PV5371 PI
PIK3C2B
PI3K-C2 beta
PV5374 (20 μg) PV5375
NP_002637.2
Catalytic
(155–1383)
Insect
N-terminal GST Lipid kinase
✓
PV5371 PI
PIK3C3
hVPS34
PV5126 (20 μg) PV5127
NP_002638.2
Full
Insect
N-terminal GST Lipid kinase
✓
PV5122 PI:PS,
PV5371 PI
PIK3CA/PIK3R1 p110 alpha/p85
alpha, PI3 Kinase
PV4788 (20 μg) PV4789
PIK3CA:
NP_006209.2
PIK3R1(p110a):
NP_852664.1
Full/full
Insect
N-terminal His
/ Untagged
Lipid kinase
✓
PV5100 PIP2:PS
PIK3CD/PIK3R1 p110 delta / p85
alpha
PV5273 (20 μg) PV5274
NP_005017.2/
NP_852664.1
Full/full
Insect
N-terminal GST Lipid kinase
/ Untagged
✓
PV5100 PIP2:PS
PIK3CG
PV4786 (20 μg) PV4787
NP_002640.2
Full
Insect
N-terminal His
Lipid kinase
✓
PV5100 PIP2:PS,
PV5122 PI:PS
PIM1
PV3503
PR5298A
NP_002639
Full
Insect
C-terminal His
Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
PIM2
PV3649
PV4036
NP_006866
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
✓
PV3180
Ser/Thr 7
Peptide
PI3K gamma, p120
gamma
PKB
TOPK, SPK, NORI-3
PV4130
PV4285
NP_060962
Full
Insect
C-terminal His
Serine/
threonine
kinase
PKN1
PRK1
PV3790
PV4037
NP_998725
Full
Insect
N-terminal GST Serine/
threonine
kinase
PKN2
PRK2
PV3879
PV4197
NP_006247
Full
Insect
N-terminal GST Serine/
threonine
kinase
LanthaScreen® Tb activity assay
Antibody
Substrate
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
LanthaScreen® Eu activity assay
Antibody
Tracer
SelectScreen®
Cell-Based
Pathway Profiling
Service
LanthaScreen® cellular assay
CellSensor®
cell line
Cell line
Antibody
PV5592
Tracer 236
KNZ3091
Y9 Peptide
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KPZ3121
Y12 Peptide
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
KPZ3141
Y14 Peptide
K1587
ERK2 U2OS
PV5269/PV5270
ERK2 [pThr185/
pTyr187]
PV5596/PV5597
Eu-anti-His
PV5592
Tracer 236
KNZ1281
ST28 Peptide
K1615
AKT HEK293E
PV5584/PV5585
AKT [pThr308]
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1031
ST3 Peptide
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1231
ST23 Peptide
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV6088
Tracer 1710
K1528 PRAS40
HEK293E, K1593
PDCD4 HEK293E,
K1615 AKT
HEK293E
PV5580/PV5581
PRAS40 [pSer183],
PV5104/PV5105
PDCD4 [pSer457]
, PV5267/PV5268
AKT [pSer473]
PV5594/PV5595
Eu-anti-GST
PV6087
Tracer 314
K1528 PRAS40
HEK293E, K1593
PDCD4 HEK293E,
K1615 AKT
HEK293E
PV5580/PV5581
PRAS40 [pSer183],
PV5104/PV5105
PDCD4 [pSer457]
, PV5267/PV5268
AKT [pSer473]
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV6087
Tracer 314
PV3566 Tb-pCREB PV3508
pSer133
Fl-CREBtide
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
KPZ1261
ST26 Peptide
PV3566 Tb-pCREB PV3508
pSer133
Fl-CREBtide
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1141
ST14 Peptide
PV3566 Tb-pCREB PV3508
pSer133
Fl-CREBtide
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1181
ST18 Peptide
PV3506 Fl-PKC
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
Antibody
Omnia® assay kit
PV5594/PV5595
Eu-anti-GST
PV3560/PV3561
Tb-pSer (pPKC)
PV5692/PV5696
Eu-PY-20
Substrate
LanthaScreen® Eu Kinase Binding
Assay
PV5693/PV5863
AF647-GT
✓
K1468 T-REx™
FOXO3-DBE HeLa
27
www.invitrogen.com
Drug Discovery
Kinase
Aliase(s)
PLK1
28
10 μg
Cat. No.
1 mg
Cat. No.
Accession number
Length
Expression
Tag
Classification
SelectScreen®
Kinase Profiling
Service
Z’-LYTE® assay
Adapta® assay
Assay kit
Substrate
PV3501
PV4038
NP_005021
Full
Insect
C-terminal His
Serine/
threonine
kinase
✓
PV3802
Ser/Thr 16
Peptide
PLK2
SNK
PV4204
PV4286
NP_006613
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3802
Ser/Thr 16
Peptide
PLK3
CNK
PV3812
PV4039
NP_004064
Catalytic
(58–340)
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3802
Ser/Thr 16
Peptide
PRKACA
PKA
P2912
PV4040
NP_002721.1
Catalytic
(1–351)
E. coli
N-terminal His
Serine/
threonine
kinase
✓
PV3174
Ser/Thr 1
Peptide
PRKCA
PKC alpha
P2232 (5 μg),
P2227 (20 μg)
PV3600
NP_002728
Full
Insect
none
Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
PRKCB1
PKC beta I
P2291 (5 μg),
P2281 (20 μg)
PV3601
NP_997700.1
Full
Insect
none
Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
PRKCB2
PKC beta II
P2254 (5 μg),
P2251 (20 μg)
PV3602
NP_002729
Full
Insect
none
Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
PRKCD
PKC delta
P2293 (5 μg),
P2287 (20 μg)
PV3603
NP_006245
Full
Insect
none
Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
PRKCE
PKC epsilon
P2292 (5 μg),
P2282 (20 μg)
PV3609
NP_005391.1
Full
Insect
none
Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
PRKCG
PKC gamma
P2233 (5 μg),
P2228 (20 μg)
PV3604
NP_002730
Full
Insect
none
Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
PRKCH
PKC eta
P2633 (5 μg),
P2634 (20 μg)
PV3608
NP_006246
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
PRKCI
PKC iota
PV3183 (5 μg), PV3607
PV3186 (20 μg)
NP_002731
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
PRKCN
PKD3, PRKG3, EPK2,
PKC nu
PV3692
PV4044
NP_005804
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV4316
Ser/Thr 17
Peptide
PRKCQ
PKC theta
P2996
PV3605
NP_006248
Full
Insect
C-terminal His
Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
PRKCZ
PKC zeta
P2273 (5 μg),
P2268 (20 μg)
PV3606
NP_002735
Full
Insect
none
Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
PRKD1
PKD, PKC mu
PV3791
PV4041
NP_002733
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV4316
Ser/Thr 17
Peptide
PRKD2
PKD2, HSPC187
PV3758
PV4042
NP_057541
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV4316
Ser/Thr 17
Peptide
PRKG1
PGK, CGK1, FLJ36117 PV4340
PV4351
NP_006249
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3174
Ser/Thr 1
Peptide
PRKG2
PKG2, PRKGR2, cGKII PV3973
PV4277
NP_006250
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3174
Ser/Thr 1
Peptide
PRKX
PKX1
PV3813
PV4045
NP_005035
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3174
Ser/Thr 1
Peptide
PTK2
FAK, FADK, FAK1
PV3832
PV4085
NP_722560
Full
Insect
N-terminal GST Cytoplasmic
tyrosine kinase
✓
LanthaScreen® Tb activity assay
Antibody
Substrate
LanthaScreen® Eu activity assay
Antibody
Substrate
LanthaScreen® Eu Kinase Binding
Assay
Antibody
Omnia® assay kit
Tracer
SelectScreen®
Cell-Based
Pathway Profiling
Service
LanthaScreen® cellular assay
CellSensor®
cell line
Cell line
KNZ1051
ST5 Peptide
✓
K1635 CRE
Jurkat, K1637
CRE HEK, K1636
CRE FreeStyle™
293F, K1638 CRE
CHO-K1
PV3506 Fl-PKC
KNZ1081
ST8 Peptide
✓
K1671 NFAT
Jurkat, K1669
NFAT HEK
PV3560/PV3561
Tb-pSer (pPKC)
PV3506 Fl-PKC
KNZ1081
ST8 Peptide
✓
K1669 NFAT HEK,
K1671 NFAT Jurkat
PV3560/PV3561
Tb-pSer (pPKC)
PV3506 Fl-PKC
KNZ1081
ST8 Peptide
✓
K1669 NFAT HEK,
K1671 NFAT Jurkat
PV3560/PV3561
Tb-pSer (pPKC)
PV3506 Fl-PKC
KNZ1081
ST8 Peptide
PV3560/PV3561
Tb-pSer (pPKC)
PV3506 Fl-PKC
KNZ1081
ST8 Peptide
PV3560/PV3561
Tb-pSer (pPKC)
PV3506 Fl-PKC
KNZ1081
ST8 Peptide
PV3560/PV3561
Tb-pSer (pPKC)
PV3506 Fl-PKC
PV3560/PV3561
Tb-pSer (pPKC)
PV3506 Fl-PKC
PV3566 pCREB
Tb-pSer133
PV3508
Fl-CREBtide
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1081
ST8 Peptide
PV3560/PV3561
Tb-pSer (pPKC)
PV3506 Fl-PKC
PV5596/PV5597
Eu-anti-His
PV5592
Tracer 236
KNZ1081
ST8 Peptide
PV3560/PV3561
Tb-pSer (pPKC)
PV3506 Fl-PKC
PV3564/PV3565
Tb-Crosstide,
PV3566 Tb-pCREB
P133
PV3509
Fl-Crosstide,
PV3508
Fl-CREBtide
PV3560/PV3561
Tb-pSer (pPKC)
PV5596/PV5597
Eu-anti-His
PV5596/PV5597
Eu-anti-His
PV5592
Tracer 236
PV5592
Tracer 236
Antibody
K1490 NFAT RA-1
KNZ1081
ST8 Peptide
KNZ1081
ST8 Peptide
✓
K1671
NFAT Jurkat
KNZ1081
ST8 Peptide
PV3566 Tb-pCREB PV3508
pSer133
Fl-CREBtide
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1081
ST8 Peptide
PV3566 Tb-pCREB PV3508
pSer133
Fl-CREBtide
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1081
ST8 Peptide
PV3566 Tb-pCREB PV3508
pSer133
Fl-CREBtide
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1051
ST5 Peptide
PV3566
PV3508
Tb-pCREB pSer133 Fl-CREBtide
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1051
ST5 Peptide
PV3560/PV3561
Tb-pSer (pPKC)
PV3506 Fl-PKC
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1051
ST5 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ3031
Y3 Peptide
PV5692/PV5696
Eu-PY-20
V5693/PV5863
AF647-GT
29
www.invitrogen.com
Drug Discovery
Kinase
Aliase(s)
1 mg
Cat. No.
Accession number
Length
Expression
Tag
Classification
SelectScreen®
Kinase Profiling
Service
Z’-LYTE® assay
Adapta® assay
Assay kit
Substrate
PTK2B
FAK2, CAKB, PYK2
PV4567
PV4568
NP_004094
Full
Insect
N-terminal GST Cytoplasmic
tyrosine kinase
✓
PV3190
Tyr 1 Peptide
PTK6
Brk
PV3291
PV4086
NP_005966
Full
Insect
C-terminal His
✓
PV3190
Tyr 1 Peptide
RAF1 (cRaf)
Y340D Y341D
PV3805
PV4046
NP_002871, Y340D,
Y341D
Catalytic
(306–648)
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3176
Ser/Thr 3
Peptide
RET
PV3819
PV4119
NP_066124
Cytoplasmic
(658–1114)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3191
Tyr 2 Peptide
RET V804L
PV4397
PV4433
NP_066124, V804L
Cytoplasmic
(658–1114;
V804L)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3191
Tyr 2 Peptide
RET Y791F
PV4396
PV4432
NP_066124, Y791F
Cytoplasmic
(658–1114;
Y791F)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3191
Tyr 2 Peptide
✓
Cytoplasmic
tyrosine kinase
RIPK2
RICK, RIP2, CARD3,
CARDIAK
PV4213
PV4325
NP_003812
Full, catalytic
(1–299)
Insect
N-terminal His
RIPK5
DustyPK,
HDCMD38P,
KIAA0472, RIP5
PV4678
PV4679
NP_056190.1
Full
Insect
N-terminal GST Serine/
threonine
kinase
PV3691
PR7028A
NP_005397
Catalytic
(1–535)
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
PV3759
PV4048
NP_004841
Catalytic
(1–552)
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3793
Ser/Thr 13
Peptide
PV3861
PV4179
NP_004551
Cytoplasmic
(425–923)
Insect
N-terminal GST Receptor
tyrosine kinase
ROCK1
ROCK2
KIAA0619
ROR2
Serine/
threonine
kinase
ROS1
ROS, MCF3
PV3814
PV4120
NP_002935
Cytoplasmic
(1883–2347)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3190
Tyr 1 Peptide
RPS6KA1
RSK1, HU-1
PV3680
PV4049
NP_002944
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV3179
Ser/Thr 6
Peptide
RPS6KA2
RSK3, HU-2
PV3846
PV4050
NP_066958
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV3179
Ser/Thr 6
Peptide
RPS6KA3
RSK2, HU-3
PV3323
PV4051
NP_004577
Full
Insect
C-terminal His
Serine/
threonine
kinase
✓
PV3179
Ser/Thr 6
Peptide
RPS6KA4
MSK2, RSK-B
PV3782
PV4052
NP_003933
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3174
Ser/Thr 1
Peptide
RPS6KA5
MSK1, RLPK
PV3681
PV4053
NP_004746.2
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3174
Ser/Thr 1
Peptide
RPS6KA6
RSK4
PV4557
PV4558
NP_055311
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV4532
Ser/Thr 20
Peptide
RPS6KB1
p70S6K, STK14A,
S6K
PV3815
PV4054
NP_003152, T412E
Catalytic
(1–421)
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
RPS6KB2
p70S6K beta, P70beta, S6K2
PV3831
PV4055
NP_003943
Full
Insect
N-terminal GST Serine/
threonine
kinase
SGK
SGK1
PV3818
PV4056
NP_005618, S589D
Catalytic
(60–431)
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3179
Ser/Thr 6
Peptide
PV3858
PV4057
NP_057360, S416D
Catalytic
(54–427)
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3179
Ser/Thr 6
Peptide
SGK2
30
10 μg
Cat. No.
LanthaScreen® Tb activity assay
Antibody
Substrate
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV3552/PV3553
Tb-PY-20
LanthaScreen® Eu activity assay
Antibody
PV5692/PV5696
Eu-PY-20
Substrate
PV5693/PV5863
AF647-GT
LanthaScreen® Eu Kinase Binding
Assay
Antibody
Omnia® assay kit
Tracer
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5596/PV5597
Eu-anti-His
PV5593
Tracer 178
PV4813/PV4814
Tb-pMAP2K1
PV4812
Fl-MAP2K1
PV5594/PV5595
Eu-anti-GST
PV5593
Tracer 178
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KPZ3121
Y12 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ3081
Y8 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ3111
Y11 Peptide
PV5596/PV5597
Eu-anti-His
PV5830
Tracer 199
PV3560/PV3561
Tb-pSer (pPKC)
PV3506 Fl-PKC
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1181
ST18 Peptide
PV3560/PV3561
Tb-pSer (pPKC)
PV3506 Fl-PKC
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1051
ST5 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ3041
Y4 Peptide
PV3560/PV3561
Tb-pSer (pPKC)
PV3506 Fl-PKC
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
KNZ1061
ST6 Peptide
PV3560/PV3561
Tb-pSer (pPKC)
PV3506 Fl-PKC
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
KNZ1061
ST6 Peptide
PV3564/PV3565
Tb-Crosstide
PV3509
Fl-Crosstide
PV5596/PV5597
Eu-anti-His
PV5592
Tracer 236
KNZ1061
ST6 Peptide
PV3566 Tb-pCREB PV3508
pSer133
Fl-CREBtide
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV3566 Tb-pCREB PV3508
pSer133
Fl-CREBtide
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1051
ST5 Peptide
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1181
ST18 Peptide
PV3564/PV3565
Tb-Crosstide
PV3509
Fl-Crosstide
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1181
ST18 Peptide
PV3560/PV3561
Tb-pSer (pPKC)
PV3506 Fl-PKC
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ1011
ST1 Peptide
PV3560/PV3561
Tb-pSer (pPKC)
PV3506 Fl-PKC
SelectScreen®
Cell-Based
Pathway Profiling
Service
CellSensor®
cell line
LanthaScreen® cellular assay
Cell line
Antibody
KNZ1041
ST4 Peptide
31
www.invitrogen.com
Drug Discovery
Kinase
Aliase(s)
1 mg
Cat. No.
Accession number
Length
Expression
Tag
Classification
SelectScreen®
Kinase Profiling
Service
Z’-LYTE® assay
Adapta® assay
Assay kit
Substrate
SGKL
SGK3
PV3859
PV4058
NP_037389, S487D
Catalytic
(87–496)
Insect
N-terminal GST Serine/
threonine
kinase
✓
SLK
KIAA0204, STK2,
se20-9
PV3830
PV4059
NP_055535.1
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
SNF1LK2
Salt-inducible
kinase, QIK, SIK2
PV4792
PV4793
NP_056006.1
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
SPHK1
PV5214 (20 μg) PV5215
NP_068807.2
Full
Insect
N-terminal GST Lipid kinase
✓
PV5372
Sphingosine
Substrate
SPHK2
PV5216 (20 μg) PV5217
NP_064511.2
Full
Insect
N-terminal GST Lipid kinase
✓
PV5372
Sphingosine
Substrate
P3044
PV3584
NP_005408
Full
Insect
C-terminal His
Cytoplasmic
tyrosine kinase
✓
PV3191
Tyr 2 Peptide
P2904
PV4087
NP_005408
Full
Insect
none
Cytoplasmic
tyrosine kinase
✓
PV3191
Tyr 2 Peptide
SRC
ASV
SRCN1
PV3179
Ser/Thr 6
Peptide
PV5166
Ser/Thr 25
Peptide
SRMS
SRM
PV4214
PV4287
NP_543013
Full
Insect
N-terminal GST Cytoplasmic
tyrosine kinase
✓
PV3190
Tyr 1 Peptide
SRPK1
SFRSK1
PV4215
PV4326
NP_003128
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV4319
Ser/Thr 18
Peptide
SRPK2
SFRSK2
PV3829
PV4060
NP_872633
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
STK16
MPSK, TSF1, PKL12
PV4311
PV4442
NP_003682
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
STK17A
DRAK1
PV3783
PV4061
NP_004751
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
STK22B
TSSK2
PV3622
PV4062
NP_443732
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV3177
Ser/Thr 4
Peptide
STK22D
TSSK1, FKSG81,
SPOGA4
PV3505
PV4063
NP_114417
Full
Insect
C-terminal His
Serine/
threonine
kinase
✓
PV3177
Ser/Thr 4
Peptide
STK23
MSSK1
PV3880
PV4198
NP_055185
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV4319
Ser/Thr 18
Peptide
STK24
MST3, STK3
PV3650
PV4064
NP_003567
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
STK25
YSK1, SOK1
PV3657
PV4065
NP_006365
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
STK3
MST2
PV4805
PV4806
NP_006272.2
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
PV4343
PV4354
NP_112168
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
PV3854
PV4177
NP_006273
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV3180
Ser/Thr 7
Peptide
PV3857
PV4089
NP_003168
Full
Insect
N-terminal GST Cytoplasmic
tyrosine kinase
✓
PV3191
Tyr 2 Peptide
STK33
STK4
SYK
32
10 μg
Cat. No.
MST1, KRS2, MST1
LanthaScreen® Tb activity assay
Antibody
PV3560/PV3561
Tb-pSer (pPKC)
Substrate
LanthaScreen® Eu activity assay
Antibody
Substrate
PV3506 Fl-PKC
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
LanthaScreen® Eu Kinase Binding
Assay
Antibody
Omnia® assay kit
Tracer
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
SelectScreen®
Cell-Based
Pathway Profiling
Service
CellSensor®
cell line
LanthaScreen® cellular assay
Cell line
Antibody
KNZ1041
ST4 Peptide
KNZ3021
Y2 Peptide
KPZ3121
Y12 Peptide
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KPZ1311
ST31 Peptide
PV3566 pCREB
Tb-pSer133
PV3508
Fl-CREBtide
PV3560/PV3561
Tb-pSer (pPKC)
PV3506 Fl-PKC
PV3560/PV3561
Tb-pSer (pPKC)
PV3506 Fl-PKC
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
KNZ3071
Y7 Peptide
K1490
NFAT RA-1
33
www.invitrogen.com
Drug Discovery
Kinase
Aliase(s)
1 mg
Cat. No.
Accession number
Length
Expression
Tag
Classification
SelectScreen®
Kinase Profiling
Service
TAOK2
TAO1, PSK, TAO2,
KIAA0881
PV3760
PV4068
NP_004774
Catalytic
(1–314)
Insect
N-terminal GST Serine/
threonine
kinase
✓
TAOK3
JIK
PV3652
PV4069
NP_057365
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
TBK1
NAK, T2K, FLJ11330
PV3504
PV4070
NP_037386
Full
Insect
N-terminal His
Serine/
threonine
kinase
✓
TEC
PSCTK4
PV3269
PV4090
NP_003206
Full
Insect
C-terminal His
Cytoplasmic
tyrosine kinase
✓
TEK
Tie2, VMCM,
CD202B
PV3628
PV4199
NP_000450
Cytoplasmic
(817–1101)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
TGFBR1
ALK5
PV5837
PV5838
NP_004603.1
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
TNK2
ACK
PV4807
PV4808
NP_005772.3
Cytoplasmic
(110–476)
Insect
N-terminal GST Cytoplasmic
tyrosine kinase
✓
TTK
MPS1L1
PV3792
PV4071
NP_003309
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
PV5860
PV5861
NP_003319.2
Full
Insect
N-terminal GST Cytoplasmic
tyrosine kinase
✓
TXK
Z’-LYTE® assay
Adapta® assay
Assay kit
Substrate
PV3180
Ser/Thr 7
Peptide
PV3178
Ser/Thr 5
Peptide
PV3676
Tyr 5 Peptide
TYK2
JTK1
PV4790
PV4791
NP_003322.2
Catalytic
(833–1187)
Insect
N-terminal GST Cytoplasmic
tyrosine kinase
✓
PV3192
Tyr 3 Peptide
TYRO3
DKT, BRT, TIF
PV3828
PV4121
NP_006284
Cytoplasmic
(451–890)
Insect
N-terminal GST Receptor
tyrosine kinase
✓
PV3191
Tyr 2 Peptide
WEE1
PV3817
PV4072
NP_003381.1
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
WNK2
NY-CO-43, KIAA1760 PV4341
PV4352
NP_006639.3
Catalytic
(166–489)
Insect
N-terminal GST Serine/
threonine
kinase
✓
WNK3
PV4342
PV4353
NP_065973
Catalytic
(1–434)
Insect
N-terminal His
Serine/
threonine
kinase
P3078
PV4091
NP_005424
Full
Insect
C-terminal His
Cytoplasmic
tyrosine kinase
ZAK
PV3882
PV4200
NP_598407
Full
Insect
N-terminal GST Serine/
threonine
kinase
✓
ZAP70
P2782
PR3017A
NP_001070
Full
Insect
C-terminal His
✓
YES1
34
10 μg
Cat. No.
Yes, C-YES, P61-Yes
Cytoplasmic
tyrosine kinase
✓
PV3191
Tyr 2 Peptide
PV4305
Tyr 7 Peptide
LanthaScreen® Tb activity assay
Antibody
Substrate
LanthaScreen® Eu activity assay
Antibody
Substrate
LanthaScreen® Eu Kinase Binding
Assay
Antibody
Tracer
PV3560/PV3561
Tb-pSer (pPKC)
PV3506 Fl-PKC
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV3564/PV3565
Tb-Crosstide
PV3509
Fl-Crosstide
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV3560/PV3561
Tb-pSer (pPKC)
PV3506 Fl-PKC
PV5596/PV5597
Eu-anti-His
PV5592
Tracer 236
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5596/PV5597
Eu-anti-His
PV5593
Tracer 178
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV5594/PV5595
Eu-anti-GST
PV5593
Tracer 178
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV5594/PV5595
Eu-anti-GST
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV4844/PV4845
Tb-STAT1
PV5211
GFP-STAT1
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV5693/PV5863
AF647-GT
Omnia® assay kit
SelectScreen®
Cell-Based
Pathway Profiling
Service
CellSensor®
cell line
Cell line
✓
K1674
ISRE RA-1
✓
K1671
NFAT Jurkat
PV5592
Tracer 236
✓
K1667
NFkB ME-180
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
✓
K1655
ISRE HEK
PV5594/PV5595
Eu-anti-GST
PV5592
Tracer 236
PV5594/PV5595
Eu-anti-GST
PV5593
Tracer 178
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV6088
Tracer 1710
✓
K1671
NFAT Jurkat
KNZ1121
ST12 Peptide
LanthaScreen® cellular assay
Antibody
KPZ3121
Y12 Peptide
KNZ3061
Y6 Peptide
KNZ3051
Y5 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
KNZ3051
Y5 Peptide
PV3552/PV3553
Tb-PY-20
PV3611 Fl-GAT/
PV3610 Fl-GT
PV5692/PV5696
Eu-PY-20
PV5693/PV5863
AF647-GT
PV6089/PV6090
Biotin-anti-His
and PV5899/
PV6025 Eu-SA
PV5592
Tracer 236
KNZ3081
Y8 Peptide
35
www.invitrogen.com
Drug Discovery
Biochemical kinase assay technology overview
Robust and reliable assays to advance your kinase
research
Table 1. Biochemical kinase assay comparison guide.
Application
Primary screening
LanthaScreen® activity assay
Invitrogen continues to design, develop, and deliver unique and
Ideal primary screening technology due to lack of
compound interference; universal tyrosine kinase
assay; tested with 165 kinases
robust assay technologies to meet the demands of the drug discovery community. We have developed a variety of preconfigured fluorescent assay solutions to meet your target validation,
assay development, and screening needs.
All Invitrogen kinase assay technologies are nonradioactive,
homogeneous, highly miniaturizable, with Z´-factors >0.5, and
are available as kits, custom, or bulk reagents. We offer unique
Workflow application
High-throughput screening/profiling
Total assay volume (kinase and detection)
20 μL
Ratiometric readout
Yes
Detection technology
TR-FRET
Increase-in-signal assay
Yes
Kinetic vs. endpoint readout
Endpoint
Antibody-based assay
Yes
and robust assay technologies optimized for high-throughput
Detection step required
Yes (antibody)
screening, including:
Reaction time
30–60 min
→
Assay stability/read window
Stable for up to 18 hr
Kinases tested
See www.invitrogen.com/lanthascreen
Overcoming interference
Time-resolved readout allows read after interfering
signals have decayed
LanthaScreen® activity assays
→
LanthaScreen® Eu Kinase Binding Assays
→
Adapta® Universal Kinase Assay
→
Z´-LYTE® FRET assays
ATP concentration flexibility
Complete flexibility; tested up to 1 mM
→
Omnia® real-time kinetic assays
Substrate concentration flexibility
100 nM–1 μM
Compatibility with protein substrates
Yes
Compatibility with cell lysates
Yes
Instruments validated by Invitrogen*
BioTek Instruments Synergy™ 4 and Synergy™ 2;
BMG LABTECH PHERAstar/PHERAstar Plus and
Omega; Molecular Devices Analyst®, FlexStation®
and SpectraMax® M5/M5e; PerkinElmer EnVision®,
VICTOR™ and ViewLux™; Tecan Infinite® F500,
Infinite® M1000 and Safire2™; Thermo Scientific
Varioskan® Flash
Use the biochemical kinase assay comparison guide (Table 1) to
select the right Invitrogen technology to help you achieve your
research goals.
36
Primary screening or profiling of all epitopetagged kinases, including unactive or lowactivity kinases
Primary or secondary screening of difficult
targets
Secondary screening
Mechanistic studies
LanthaScreen® Eu Kinase Binding Assay
Adapta® assay
Z´-LYTE® assay
Omnia® assay
Ideal for screening of non-activated or lowactivity kinases due to direct binding readout; no
detection step means slow binding inhibitors can
be easily examined. Also ideal for detection of
non–Type I kinase inhibitors or when no substrate
is available.
Ideal for screening when the target of interest does
not phosphorylate traditional peptide substrates;
validated specifically for lipid kinase projects
Ideal for secondary screening, hit validation,
and profiling because this technology has been
validated with >230 kinases; primary technology
platform for SelectScreen® Kinase Profiling Service
Ideal for mechanistic studies and measuring
kinase activity in cell lysates; simple mix-and-read
fluorescence-based assay allows measurements in
real time (kinetics)
High-throughput screening/profiling
High- to medium-throughput screening projects/
secondary screening/profiling
Medium-throughput screening/secondary
screening/assay development/profiling
Downstream of high-throughput screening
15 μL
15 μL
20 μL
20–100 μL
Yes
Yes
Yes
No
TR-FRET
TR-FRET
FRET
Fluorescence intensity
Yes
No
No
Yes
Endpoint or kinetic
Endpoint
Endpoint
Kinetic
Yes
Yes
No
No
Yes (antibody)
Yes (antibody)
Yes (protease)
No
0–90 min
30–60 min
2 hr
Seconds to 120 min
Stable up to 24 hr
Stable for up to 18 hr
4–18 hr (kit-dependent)
Up to 2 hr (real-time measurement)
See www.invitrogen.com/bindingassay
See www.invitrogen.com/adapta
See www.invitrogen.com/zlyte
See www.invitrogen.com/omnia
Time-resolved readout allows read after interfering
signals have decayed; use of epitope-tag ensures
no contaminating kinase is detected
Time-resolved readout allows read after interfering
signals have decayed, red Alexa Fluor® dye as
acceptor further enhances ability to overcome
interference
Preread at 445/520 nm
Kinetic, real-time measurement
ATP not needed for binding analysis
Tested from 1 to 500 μM
Complete flexibility; tested up to 1 mM
Complete flexibility; tested up to 1 mM
No substrate needed
Complete flexibility, must be greater than ATP
concentration
2 μM only
Complete flexibility
NA
Yes
No
No
No
Yes
BioTek Instruments Synergy™ 4, Synergy™ 2,
Synergy™ HT and FLx800™; BMG LABTECH
PHERAstar/PHERAstar Plus, Omega and OPTIMA;
Molecular Devices Analyst®, FlexStation® and
SpectraMax® M5/M5e; PerkinElmer EnVision®;
Tecan Infinite® F500, Infinite® M1000 and Safire2™;
Thermo Scientific Varioskan® Flash
BioTek Instruments Synergy™ 4, Synergy™ 2,
Synergy™ HT and FLx800™; BMG LABTECH
PHERAstar/PHERAstar Plus, Omega and OPTIMA;
Molecular Devices Analyst®, FlexStation® and
SpectraMax® M5/M5e; PerkinElmer EnVision® and
VICTOR™; Tecan Infinite® F500, Infinite® M1000 and
Safire2™; Thermo Scientific Varioskan® Flash
No
No
BioTek Instruments Synergy™ 4 and Synergy™ 2;
BMG LABTECH PHERAstar/PHERAstar Plus and
Omega; Molecular Devices Analyst®, FlexStation®
and SpectraMax® M5/M5e; PerkinElmer EnVision®,
VICTOR™ and ViewLux™; Tecan Infinite® F500,
Infinite® M1000 and Safire2™; Thermo Scientific
Varioskan® Flash
BioTek Instruments Synergy™ 4 and Synergy™ 2;
BMG LABTECH PHERAstar/PHERAstar Plus and
Omega; Molecular Devices Analyst®, FlexStation®
and SpectraMax® M5/M5e; PerkinElmer EnVision®,
VICTOR™ and ViewLux™; Tecan Infinite® F500,
Infinite® M1000 and Safire2™; Thermo Scientific
Varioskan® Flash
* As they become available, setup instructions for each instrument can be found at www.invitrogen.com/instrumentsetup.
37
www.invitrogen.com
Drug Discovery
LanthaScreen® activity assays—a powerful toolbox
for TR-FRET kinase activity assay development
Every assay application has its own specific requirements. To get
amount of phosphorylated substrate present. In this manner,
the assay components you need without the ones you don’t, our
kinase activity can be detected and measured by an increase in
LanthaScreen® activity assay reagents are available as stand-alone
the emission ratio.
products. The LanthaScreen® toolbox for kinase activity assays
As an extension to our LanthaScreen® peptide-based sub-
includes:
strates, we also offer physiological protein substrates that are
→
Terbium- and europium-labeled phosphospecific antibodies*
directly labeled with either GFP or fluorescein. These assays func-
→
Fluorescein- and Alexa Fluor® 647–labeled peptide substrates
tion using the same mechanism as the peptide-based substrate
→
GFP- or fluorescein-labeled protein substrates
assay, but allow LanthaScreen® assays to be developed for kinases
→
Terbium- and europium-labeled anti-epitope and secondary
that preferentially or exclusively act on the physiological protein
antibodies*
rather than peptide substrate.
See the LanthaScreen® activity assay conditions for your
TR-FRET assays offer advantages over other fluorescent assay
kinase at www.invitrogen.com/lanthascreenkinase.
formats when background fluorescence can lead to false
negative or false positive results, depending on the assay
configuration. Because the donor species used in our TR-FRET
Interfering signal
longer than background fluorescence or scattered light (Figures
2 and 3), energy transfer can be measured after the interfering
Intensity
assay format has a fluorescence lifetime orders of magnitude
Signal due to Tb or Eu
signal has completely decayed.
In a LanthaScreen® kinase activity assay, a terbium (Tb)- or
europium (Eu)-labeled phosphospecific antibody binds the phosphorylated fluorescein- or Alexa Fluor® 647–labeled substrate,
Excitation
Delay
Measure
Time (μsec)
resulting in an increased TR-FRET value (Figure 4). The amount
of antibody that binds to the substrate is proportional to the
* Please contact us at discoverysciences@invitrogen.com to access europiumlabeled antibodies.
38
Figure 2. Schematic of measurement setup for detection of TR-FRET in HTS assays.
The LanthaScreen® kinase activity assay format is based on the use of a longlifetime terbium or europium chelate as the donor species and fluorescein
as the acceptor species. When Tb- and fluorescein-labeled or Eu- and Alexa
Fluor® 647–labeled molecules are brought into proximity, energy transfer
takes place, causing an increase in acceptor fluorescence and a decrease in
donor fluorescence. These fluorescent signals can be read in a time-resolved
manner to reduce assay interference and increase data quality.
A
B
O Coumarin
O Fluorescein
100
90
Excitation/Emission
Absorbance
1.00
1.1
110
O NADPH
O Tartrazine
O Allura Red AC
0.75
0.50
0.25
70
0.8
60
50
0.7
40
0.6
30
20
0.5
10
0.00
0
300
400
500
600
1.0
0.9
80
300
Wavelength (nm)
350
400
450
500
550
600
650
0.4
0.3
Wavelength (nm)
0.2
1
3
2
4
5
0.1
6
nt
in
at
te
ra
ce
Sc
Fl
uo
re
s
m
ar
Re
ou
C
ra
in
d
e
zin
lu
Al
PH
AD
tra
Ta
r
N
in
te
rfe N
ra o
nt
0
Figure 3. Resistance to interference from color quenchers, fluorescent compounds, and scattered light. (A) To demonstrate the resistance of the LanthaScreen® Tb format
to interference from color quenchers, fluorescent compounds, or light scattering agents, kinase assays were read in the presence of such compounds. The color
quenchers and fluorescent compounds were chosen to overlap with the excitation and emission spectra of the Tb chelates, in order to provide a worst-case scenario
for interference. The absorbance or fluorescent spectra of these compounds are shown, along with photos demonstrating the appearance of the solutions in the
assay wells. 1: 100 nM coumarin; 2: 100 nM fluorescein; 3: 500 μg/mL nondairy creamer; 4: buffer blank; 5: 10 μM tartrazine; 6: 10 μM Allura Red AC. (B) Src kinase was
assayed in the absence and presence of interfering compounds. Any interference seen in the raw data was compensated for by the ratiometric nature of the readout.
Kinase reaction
Add antibody and detect
Kinase
Tb
P
ATP
Fluorescein
P
Uninhibited reaction
High TR-FRET
Kinase
Tb
Fluorescein
Inhibited reaction
ATP
inhibitor
Low TR-FRET
Figure 4. LanthaScreen® kinase activity assay using traditional peptide substrates. A fluorescein-labeled kinase substrate peptide is incubated with kinase and ATP. Terbiumor europium-labeled antibody is then added and phosphorylation detected by an increase in the TR-FRET ratio. Because the substrate is directly labeled, there is no
need to add streptavidin-APC. Additionally, unlike other europium-based systems, there is no requirement to add high concentrations of potassium fluoride, which
can potentially disrupt antibody–product interactions.
39
www.invitrogen.com
Drug Discovery
Bring versatility to your search for novel kinase
inhibitors using the LanthaScreen® Eu Kinase
Binding Assay
In the LanthaScreen® Eu Kinase Binding Assay, binding of an Alexa
→
Fluor® conjugate or “tracer” to a kinase is detected by addition of
a europium-labeled anti-tag antibody. Binding of the tracer and
Measure the right kinase with an intrinsically validated
system
→
antibody to a kinase results in a high degree of FRET, whereas dis-
Obtain sensitive detection of kinase inhibitors, including
allosterics
placement of the tracer with a kinase inhibitor results in a loss of
→
Interrogate active and non-activated kinases
FRET (Figure 5). Unlike many kinase activity assays, this is an easy-
→
Track slow-binding inhibitors in real time
to-optimize assay, with no development steps required. With this
→
Compare on-off rates of your targets
assay you can:
Step 1: Mix kinase, antibody, tracer, and compound
Step 2: Detect
Eu
Eu
Tag
Alexa
Fluor®
647
Kinase
Tracer
Tag
Compound
(non-inhibitor)
Kinase
High TR-FRET
Eu
Eu
Tag
Kinase
Tag
Tracer
Compound
(inhibitor)
Kinase
Low TR-FRET
Figure 5. Schematic of the LanthaScreen® Eu Kinase Binding Assay.
40
A challenge often faced when developing kinase activity
The LanthaScreen® Eu Kinase Binding Assay is an easy-to-opti-
assays, especially for less-studied targets and those with low spe-
mize assay ideal for assay development and multiple downstream
cific activity, is ensuring that the measured activity is due to the
applications. Invitrogen has validated more than 200 kinases for
target of interest rather than low levels of contaminating kinases.
use with this assay, and provides detailed assay setup instruc-
This is particularly true when using “generic” substrates and when
tions for individual kinases. To see if your kinase is on the list, visit
specific control inhibitors are unavailable. Any signal resulting
www.invitrogen.com/bindingassaytable.
from the LanthaScreen® Eu Kinase Binding Assay is due to specific
interaction of a Eu-anti-epitope-tagged antibody bound to the
kinase of interest.
pound that binds to the ATP site, including both Type I inhibitors
(which bind only the ATP site) and Type II inhibitors (which bind
Normalized signal
Invitrogen kinase tracers are based on ATP-competitive
kinase inhibitors, making them suitable for detection of any com-
Abl
(IC50 = 190 nM)
1.2
Abl, dephosphorylated
(IC50 = 5 nM)
1.0
.8
.6
.4
the ATP site and a second site often referred to as the allosteric
.2
site) such as Gleevec® (imatinib), sorafenib, or BIRB-796. Unlike
0
10-2
10-1
101
1
activity assays, the kinase binding assay can be performed with
either active or non-activated kinase preparations, making it ideal
An additional property of some so-called “allosteric” inhibitors is a slow compound on-rate due to the compound preferen-
Normalized signal
for detection of Type II inhibitors, which often show a higher affinity for nonactiviated kinases (Figure 6).
Abl, dephosphorylated
(IC50 = 320 nM)
.8
.6
.4
0
Kinase Activity Assay, one can measure the binding over time by
105
1.0
with other forms. Although this can be measured by monitoring
directly. Since there is no detection step in the LanthaScreen® Eu
104
Abl
(IC50 = 120 nM)
.2
and inhibitor, it is substantially simpler to monitor this binding
103
1.2
tially binding to a conformation of the kinase that is in equilibrium
the activity at various time points after pre-incubating the kinase
102
[Gleevec®] (nM)
10-1
1
101
102
103
104
105
[Staurosporine] (nM)
Figure 6. Preferential binding of the Type II inhibitor Gleevec® to unphosphorylated
Abl. (A) The affinity of Gleevec® was measured using the binding assay by mixing His-tagged Abl (1 nM), both with and without phosphatase treatment,
with 0.25 nM of Kinase Tracer 178 and 2 nM Eu-anti-His-Tag antibody followed
by TR-FRET measurements. (B) The same experiment was conducted using
staurosporine instead of Gleevec®.
taking multiple reads of the same assay wells.
41
www.invitrogen.com
Drug Discovery
Study difficult targets, such as lipid kinases, with
the Adapta® Universal Kinase Assay Kit
The Adapta® Universal Kinase Assay Kit is a homogeneous, fluo-
→
rescence-based immunoassay for the detection of ADP. The ben-
→
High Z´-factors at low percent conversion of ATP to ADP
→
One assay suitable for all kinases, including difficult-to-assay
→
rescence or light scattering from precipitated compounds
→
efits of this assay format include:
Allows for reduced interference from compound autofluo-
Complete validated assay system available including substrate and specialized assay buffer
targets such as lipid kinases
The principle of the Adapta® Universal Kinase Assay is shown
Best-in-class Alexa Fluor® dyes for a superior red-shifted TR-
in Figure 7. The assay itself is divided into two phases: a kinase
FRET assay
reaction phase, and an ADP detection phase. In the kinase reac-
Add antibody
and tracer
Kinase reaction
Detection
Eu
Eu
ATP
ADP
ADP
Kinase
ATP
Substrate
ADP
ADP
ATP
ADP
Alexa
Fluor®
647
ADP
Tracer
Uninhibited reaction
Low TR-FRET
Eu
Eu
ATP
ATP
ATP
ATP
Kinase
inhibitor
ATP
ATP
Substrate
ATP
Tracer
Inhibited reaction
Figure 7. Schematic diagram showing the Adapta® Universal Kinase Assay.
42
ATP
Alexa
Fluor®
647
ATP
High TR-FRET
tion phase, all components required for the kinase reaction are
Our lipid substrates were designed for optimal performance
added to the well, and the reaction is allowed to incubate for a set
in the Adapta® Universal Kinase Assay with our PI3 kinases.
period of time (typically 60 minutes). After the reaction, a detec-
→
tion solution consisting of a europium-labeled anti-ADP antibody,
an Alexa Fluor® 647 dye–labeled ADP tracer, and EDTA (to stop
substrates are ready for immediate use in your kinase assay
→
the kinase reaction) is added to the assay well. ADP formed by
the kinase reaction (in the absence of an inhibitor) will displace
No rehydration, sonication, or extrusion needed—the lipid
Preformulated for optimal performance with the respective
lipid kinases
→
Get reliable, reproducible performance each and every time
the Alexa Fluor® 647 dye–labeled ADP tracer from the antibody,
resulting in a decrease in the TR-FRET signal. In the presence of
For information on instrumentation for the Adapta® Assay and to
an inhibitor, the amount of ADP formed by the kinase reaction
see if your kinase of interest has been validated using the Adapta®
is reduced, and the resulting intact antibody-tracer interaction
Universal Kinase Assay, please visit www.invitrogen.com/adapta.
results in a high TR-FRET signal.
The Adapta® Universal Kinase Assay measures kinase activity
by detecting ADP formation. The assay response is such that the
1.2
1.1
majority of the signal change occurs in the first 10–20% conver-
20% conversion
1.0
sion of ATP to ADP (Figure 8). This is in sharp contrast to kinase
ATP to ADP results in only a 20% signal change. As a result, the
Adapta® Universal Kinase Assay produces high Z´-factors at low
Emission ratio
assays that measure ATP depletion, in which 20% conversion of
0.9
Change in signal at
20% conversion
0.8
0.7
0.6
0.5
0.4
percent conversions and is ideally suited for use with kinases with
0.3
low activity, since less ADP has to be formed to achieve an opti-
0.1
0.2
0
mal assay window.
In addition to the Adapta® Universal Assay Kit, we offer
a selection of peptide- and lipid-based substrates for use with
the Adapta® assay. Both the peptide and lipid substrates were
designed with assay performance in mind.
0.01
0.1
1
10
100
Percent conversion (ATPAADP)
Figure 8. Assay signal as a function of percent conversion of ATP to ADP. A representative ATP–ADP titration curve highlights the large change in assay signal
at only 20% conversion of ATP to ADP. This makes the Adapta® assay ideally
suited for use with kinases with low activity since less ADP has to be formed
to achieve an optimal assay window.
43
www.invitrogen.com
Drug Discovery
Simple ratiometric inhibitor screening with the
Z´-LYTE® FRET-based kinase assay platform
→
→
phorylation. Complete flexibility in ATP concentrations allows for
the detection of both ATP-competitive and allosteric inhibitors.
Nonradioactive, antibody-independent format allows you to
If your kinase of interest is not listed online, and if you know the
save time and money while providing a safer assay
sequence of the peptide needed, a custom Z´-LYTE® peptide can
Flexible design allows you to vary ATP levels to detect your
be synthesized and validated for you.
compound’s mechanism of action
→
Ratiometric data readout, excellent Z´-factor values (>0.5), and
performance-validated reagents enable accurate results
Measure kinase activity in real time with
Omnia® kinase assays
Z´-LYTE® FRET-based kinase assay kits are fast, simple, nonradioac-
Omnia® kinase assays measure kinase activity in real time, elimi-
tive, and accurate. The Z´-LYTE® Kinase Assay Kits enable accurate
nating the need for multiple endpoint assays and saving time,
and reliable screening of potential kinase inhibitors and further the
money, and valuable samples. Kinetic data can be collected for
assessment of their selectivity profiles. These kits use the broad-
specific kinases using recombinant proteins or crude cell lysates.
est fluorescent kinase assay technology available, and are suited
The assays are fast and easy to perform, without radioactivity
to both serine/threonine and tyrosine kinases. More than 230
or specialized equipment. You get the same reliability as from
kinases have been validated using the technology (find the list
radioactive filter-binding assays, but without the exposure and
at www.invitrogen.com/zlyte), and it is the primary platform for
hassle of hazardous materials. Omnia® kinase assays offer several
our SelectScreen® biochemical kinase profiling service. The for-
key advantages:
mat is based on FRET between coumarin and fluorescein (Figure
→
9). Ratiometric measurements result in a low percent coefficient
of variation, which allows for high Z´-factors at low percent phos-
ing time, money, and sample
→
Flexibility—use recombinant kinases or crude cell lysates
Development reaction
Substrate
Emission ratio (445
ATP
Kinase
ADP
10–40% of substrate, phosphorylated
Detection
/520 )
Kinase reaction
Real-time results—collect kinetic data in one reaction, sav-
Development Reagent is added, which recognizes and
cleaves nonphosphorylated peptide
Phosphorylated
peptide
Nonphosphorylated
peptide
Figure 9. Z´-LYTE® assay schematic. In the kinase reaction, the kinase transfers the gamma phosphate of ATP to a serine/threonine or tyrosine residue in the synthetic
peptide substrate. In the development reaction, a site-specific protease recognizes and cleaves nonphosphorylated peptides. Phosphorylated peptides remain
uncleaved by the development reagent. Cleavage disrupts FRET between the coumarin and fluorescein fluorophores on the peptide, whereas uncleaved phosphorylated peptides maintain FRET. A ratiometric method, which calculates the ratio of coumarin emission over fluorescein emission, quantitates the reaction progress.
44
→
Speed—get results in under an hour
of our robust kinase assay technologies (Z´-LYTE®, Adapta®, and
→
Minimal interference—overcome false positives or nega-
LanthaScreen® Eu Kinase Binding Assay) to create the premier
tives with kinetic format
outsourced service in the market. Obtain rapid profiling of your
compounds against a panel of kinases at a single concentration,
Omnia® kinase assays use the chelation-enhanced fluorophore
or choose to have more in-depth IC50 determinations performed
(CHEF) called Sox, incorporated into a kinase-specific peptide
at variable ATP concentrations.
substrate to measure phosphorylation (Figure 10). Upon phos-
A stringent validation process allows for the highest-quality
phorylation of the peptide by the kinase of interest, there is an
data possible. Every 384-well plate we screen contains multiple
increase in fluorescence proportional to the amount of substrate
controls that determine whether the plate passes or fails. These
phosphorylation. The emission spectra in Figure 11 illustrate the
include:
2–10 fold increase in fluorescence signal between the nonphos-
→
Duplicate value difference determinations, n = 2
phorylated substrate peptide (lower curve) and the fully phos-
→
Control wells to assess assay interference—protease and fluores-
phorylated peptide product (upper curve). The fluorescence
cent controls are used to alert clients about potential interference
intensity is directly proportional to the amount of peptide phos-
→
Phosphorylation controls
phorylation. As a result, Omnia® kinase assays are ideal for kinase
→
Control inhibitor IC50 determination for each kinase
inhibitor characterization and offer high sensitivity. Find out more
at www.invitrogen.com/omnia.
The service offers unparalleled customer support and service
including high-quality results, short cycle times (guaranteed two-
Screening for kinase inhibitors made easy with
the SelectScreen® Kinase Profiling Service
week turnaround), and dedicated project management. To find
out more about the SelectScreen® Kinase Profiling Service, visit
Invitrogen’s SelectScreen® Kinase Profiling Service brings together
www.invitrogen.com/kinaseprofiling.
our growing library of purified protein kinase targets and three
Excitation
360 nm
β-turn sequence
12,000
β-turn sequence
10,000
Sox
S/T/Y
OH
O
Fluorescence (RFU)
Sox
S/T/Y
Mg2+
ion
gn
it
ase
Mg2+
rec
o
kinase, ATP
kin
kin
ase
rec
o
gn
it
ion
mo
mo
tif
tif
P
Emission
485 nm
8,000
Phosphorylated
6,000
4,000
2,000
Nonphosphorylated
0
410
450
490
530
570
610
Wavelength (nm)
Figure 10. The Omnia® kinase assay principle. Upon phosphorylation of the substrate, Mg2+ is chelated to form
a bridge between the Sox moiety and the phosphate group. Excitation at 360 nm results in an increase in
fluorescence emission at 485 nm.
Figure 11. Emission spectra of the Omnia® kinase
assays.
45
www.invitrogen.com
Drug Discovery
Cellular pathway analysis assay comparison table
Elucidate your target pathways with multiple assay
formats
In drug discovery, there is a need for tools to analyze compound
Use the cellular pathway analysis assay comparison guide
efficacy in a pathway-specific physiological context. To address
(Table 2) to help you select the right Invitrogen technology to
this need, Invitrogen offers two technology platforms for end-
enable your research.
point or proximity-oriented pathway analysis for use in highthroughput screening and profiling.
Table 2. Cellular pathway analysis assay comparison guide.
Application
Workflow application
CellSensor® cell lines
LanthaScreen® cellular assays
Transcriptional reporter gene assays
Posttranslational modification assays
Ideal for analysis of an entire signal transduction pathway with a single
end-point readout
Ideal for analysis of key signal transduction events within a pathway
High-throughput screening/profiling
High-throughput screening/profiling and downstream mechanistic
studies
Pathway/target coverage
See www.invitrogen.com/cellsensor
See www.invitrogen.com/lanthascreencellular
Measurement of endogenous/exogenous
pathways/targets
Endogenous
Endogenous
Total assay volume (384-well)
40 μL
40 μL
Ratiometric readout
Yes
Yes
Detection technology
FRET (coumarin to fluorescein)
TR-FRET (from terbium-labeled antibody to GFP-substrate fusion)
Kinetic vs. endpoint readout
Endpoint
Endpoint
Overcoming interference
Background suppression dye in the substrate mixture
Time-resolved readout allows read after interfering signals have decayed
Antibody-based assay
No
Yes
Constitutive assay inhibitor incubation time
16–18 hr
15–120 min
Ligand-inducible assay stimulation time
Varies (mostly 5 hr, some assays require 16 hr or longer)
Varies from 5 min to 120 min
Total assay time
7–24 hr
1–2 hr
Substrate/antibody concentration
1 μM Solution A
2 nM to 10 nM terbium-labeled antibody
Detection step required
Yes
Yes
Monochromator-based instrument compatibility
Yes
No, except for Tecan Infinite® M1000*
Instruments validated by Invitrogen†
Beckman Coulter PARADIGM™; BioTek Instruments Synergy™ 4, Synergy™
2, Synergy™ HT and FLx800™; BMG LABTECH Omega and OPTIMA;
Molecular Devices Analyst®, FlexStation® and SpectraMax® M5/M5e;
PerkinElmer EnVision®; Tecan Infinite® F200, Infinite® F500, Infinite®
M1000 and Safire2™; Thermo Scientific Varioskan® Flash
BioTek Instruments Synergy™ 4 and Synergy™ 2; BMG LABTECH
PHERAstar/PHERAstar Plus and Omega; PerkinElmer EnVision®; Tecan
Infinite® F500, Infinite® M1000
* We do not recommend the use of monochromator-based instruments, because most lack the sensitivity necessary to detect endogenously expressed GFP fusion proteins, and do not allow individual
selection of bandwidths used for the emission signals, further compromising signal detection.
† As they become available, setup instructions for each instrument can be found at www.invitrogen.com/instrumentsetup.
46
Analyze disease-relevant signal transduction
pathways with CellSensor® cell lines
→
Screen for agonists and antagonists of a signaling pathway
→
Identify inhibitors of a specific protein target in a signaling
pathway
→
Counterscreen and profile for compound cellular selectivity
→
Examine key pathways in their endogenous settings
Table 3. Response elements available in CellSensor® cell lines.
AP-1
Gli
NFAT
ARE
HRE
NFκB
C/EBP
IL-1
NICD-CSL
c-fos
irf1
p53RE
CRE
ISRE
SBE
dhfr(E2F)
LEF/TCF
SIE
FOXO3-DBE
MMTV
STAT6
GAS
myc
TNF-α
CellSensor® cell lines use GeneBLAzer® beta-lactamase reporter technology to provide a rapid and sensitive method of analyzing signal
transduction pathways exposed to drug candidates or other stimuli.
Step 1: Agonist or
inhibitor treatment
Step 2: Substrate loading and detection
Unstimulated
O
S
N
O
CO2AM
Receptor
Plasma membrane
Cytoplasmic
esterases
enable analysis in a high-throughput manner.
Cytoplasm
520 nm
409 nm
Many intracellular signaling pathways converge at the transcrip-
T
FRE
Fluorescein
Coumarin
O
S
HN
tional level, where transcription factors bind to DNA enhancer ele-
S
HN
Substrate
These cell lines measure compound potency and selectivity, and
OAc
Fluorescein
Coumarin
BtO
beta-lactamase
Nucleus
S
N
O
CO2
–
Low blue (460nm) / green (530nm) ratio
Stimulated
ments that regulate downstream transcription. We have generated
Coumarin
O
S
HN
BtO
OAc
Fluorescein
Substrate
S
N
O
Agonist
CO2AM
and validated nearly 60 stable cell lines containing various response
Plasma membrane
Cytoplasmic
esterases
Cytoplasm
409 nm
elements upstream of the beta-lactamase reporter gene (Table 3).
447 nm
BLA
Fluorescein
Coumarin
When pathways leading to these specific enhancer elements are
TF
O
S
HN
activated in these cell lines, beta-lactamase is produced, which can
Nucleus
beta-lactamase
Coumarin
S
HN
–
O2C
N
CO2
O
CO2
–
–
High blue (460nm) / green (530nm) ratio
Inhibited
Fluorescein
Coumarin
OAc
O
S
HN
BtO
then be detected in a homogeneous assay with our cell-permeable,
Fluorescein
O
BLA
S
N
Substrate
S
N
O
Agonist
CO2AM
Plasma membrane
ratiometric fluorescent LiveBLAzer™-FRET B/G substrate (Figure 12).
We validate these pathway readouts using target-specific, cell-per-
Cytoplasmic
esterases
Inhibitor
cell line yields a Z´-factor of >0.6 and appropriate EC50 responses to
known agonists or antagonists. In addition, CellSensor® cell lines are
tested to ensure they are mycoplasma-negative, assayed for DMSO
tolerance up to 1% DMSO, and optimized for stimulation time, cell
density, and substrate loading time. To see the validation data for
each cell line, visit www.invitrogen.com/cellsensor.
FRE
T
Fluorescein
O
S
HN
Nucleus
result of response element optimization and functional testing. Each
Cytoplasm
520 nm
Coumarin
meable small-molecule inhibitors and/or RNAi duplexes.
All CellSensor® cell lines provide superior response profiles, a
409 nm
beta-lactamase
S
N
O
CO2
–
Low blue (460nm) / green (530nm) ratio
Figure 12. Schematic of CellSensor® reporter gene assay. CellSensor® cell lines are stably engineered to express beta-lactamase under the control of pathway-specific
response elements. The principle and methods for this assay format are simple. (1)
Minimal amount of beta-lactamase is expressed in untreated cells or cells treated
with a pathway-specific inhibitor. Stimulation of the pathway with a ligand or a constitutively active mutation of a component in the pathway leads to the activation
of downstream transcription factor(s) resulting in beta-lactamase reporter gene
expression. (2) Cells are loaded with a cell-permeable beta-lactamase FRET-based
substrate, and detection of the beta-lactamase reporter activity is measured on a
bottom-read fluorescence plate reader. Low 460 nm (blue)/530 nm (green) ratio represents low beta-lactamase activity in untreated or inhibitor-treated cells and high
460 nm (blue)/530 nm (green) ratio represents high beta-lactamase activity in the
ligand-stimulated cells or cells expressing a constitutively active pathway.
47
www.invitrogen.com
Drug Discovery
Shed light on complex biology with LanthaScreen®
cellular assays for targeted drug discovery
The cellular response to environmental cues is mediated through
sient format and in physiologically relevant cell types, target substrate
a complex network of signal transduction pathways. Alteration
genes fused to GFP can be cloned into a baculovirus chromosome
or disruption of components within these pathways often con-
and packaged in viral particles for highly effective cellular delivery via
tributes to various diseases, including cancer and metabolic and
proven BacMam technology. To develop a custom BacMam assay,
inflammatory disorders. The diversity of validated drug targets in
email us at discoveryservices@invitrogen.com.
these signal transduction cascades provides both challenges and
opportunities in drug discovery. Intermediate signaling events,
such as phosphorylation and ubiquitination, are bona fide targets for assay development. Until now, there have been a limited
Step 1: Agonist or
inhibitor treatment
Unstimulated
Step 2: Lyse cells
and detect
Low TR-FRET
Receptor
number of high-throughput screening (HTS)-compatible methods
available to interrogate, in a cell-based format, the regulated activity of enzymes responsible for posttranslational modifications.
With LanthaScreen® cellular assays you can:
→
Screen quickly with a simple and homogeneous HTS-ame-
GFP
GFP
Substrate
Substrate
Stimulated
High TR-FRET
Agonist
nable assay format
→
Interrogate the effects of your compounds on specific signaling events within a pathway
→
Analyze multiple posttranslational modiciations using physi-
Phosphate
GFP
GFP
Substrate
Substrate
ological pathway kinetics
→
Develop assays in multiple cell types using BacMam-
Inhibited
Low TR-FRET
mediated gene delivery
Inhibitor
Combining the simplicity of biochemical assays with the intricacy of
cellular systems, Invitrogen has developed TR-FRET–based assays to
GFP
Substrate
GFP
Substrate
monitor protein modification within the native cell signaling environment. By expressing the substrate of interest as a fusion with Green
Fluorescent Protein (GFP), modification-specific antibodies labeled
with the TR-FRET donor partner terbium (Tb) can be used to quantitatively monitor these modifications following cell lysis (Figure 13).
To address the need for developing LanthaScreen® assays in a tran-
48
Figure 13. Schematic of LanthaScreen® cellular assay. The principle and methods
for this assay format are simple. In the first step, phosphorylation of the kinase
substrate expressed as a GFP-fusion protein is either stimulated with an agonist
to activate the pathway, or treated with an inhibitor to interrupt the signaling
event. No protein modification occurs in unstimulated cells. In the second step,
cells are lysed in a buffer that contains a Tb-labeled phosphorylation site–specific antibody. The detection of the protein modification event is measured on a
TR-FRET–compatible plate reader. Little or no TR-FRET is observed with unstimulated or inhibited cells, whereas stimulated cell samples display high TR-FRET.
Phosphospecific ELISAs
Variable
30–60 min
Stimulate
cells
Lyse
60 min
60 min
Capture
Transfer
Wash
60 min
First antibody
PSSA
Wash
Second
antibody
Variable
Development
Wash
Read
plate
LanthaScreen® cellular assay
Variable
Stimulate
cells
60 min
Lysis and PSSA
Read plate
Figure 14. Comparison of LanthaScreen® cellular assay and phosphospecific ELISA workflow. The LanthaScreen® cellular assay platform offers a high-throughput alternative
for compound screening and profiling. In contrast to the traditional ELISA-based approach for phosphoprotein analysis, this assay format requires minimal hands-on
time and can be run in as few as 2−3 hr as opposed to the typical 6–8 hr time commitment with classical phosphospecific ELISAs. (PSSA = phosphorylation site–
specific antibodies).
workflow for a LanthaScreen® cellular assay is quick, designed
Linking technologies to understand pathway
signaling with the SelectScreen® Cell-Based
Pathway Profiling Service
for HTS format using a 384-well plate, and is highly amenable
→
Interrogate kinases in their natural cellular environment
to automation (Figure 14). This assay format has no wash steps,
→
Correlate biochemical selectivity with cellular activity
and the assays can be run following an “addition only” protocol,
→
Evaluate compound activity across multiple signaling path-
Compared to classical and established methods for phosphoprotein analysis, such as western blotting and ELISA, the
requiring little hands-on time and eliminating the need for extra
ways to identify liabilities and opportunities
manual handling and liquid transfer steps.
LanthaScreen® cellular assays are developed to allow for
Invitrogen’s SelectScreen® Cell-Based Pathway Profiling Ser-
proper pharmacology toward the target of interest. Each assay
vice uses our panel of CellSensor® cell lines to provide a novel
provides excellent response profiles, yields a Z’-factor above 0.6,
way to interrogate signal transduction pathways. Rapidly profile
and demonstrates the appropriate EC 50/IC50 responses to known
your compounds in the endogenous pathway of your choosing,
agonists or antagonists. In addition, the cell lines used in LanthaS-
selecting from multiple parameters like activator and inhibitor
creen® cellular assays are mycoplasma negative and tested for
screening modes to design your optimal project.
optimal performance under a variety of experimental conditions.
Control or reference compounds are present for each cell line
For more information on LanthaScreen® cellular assays and
we assay on every plate of client compounds, and data points for all
for a complete product listing, please visit www.invitrogen.com/
services are generated in duplicate. Experience unparalleled customer
lanthascreencellular.
support and service including high-quality results, short cycle times,
and dedicated project management. Learn more at www.invitrogen
.com/pathwayprofiling.
49
www.invitrogen.com
Drug Discovery
Customize your approach to kinase signaling with
Invitrogen custom assay development services
Invitrogen custom assays and services is ready to partner with you:
Invitrogen custom assay services offer a toolbox full of assay formats
→
1,500 validated assays developed
to interrogate kinase signaling pathways. If we don’t have a prevali-
→
Dedicated project management from inquiry to completion
dated assay to interrogate your kinase signaling pathway of interest,
→
3,500 licensed and in-licensed technologies to choose from
we will build one for you. Expand your understanding of proximal
→
High-quality standards
With a proven track record of high-quality assay development,
and distal signaling events by combining multiple assay formats.
Both biochemical and cell-based assays are available, with multiple
Contact discoveryservices@invitrogen.com to design your cus-
readouts including FRET, TR-FRET, and reporter gene assays.
tom assay.
Visit us at www.invitrogen.com/kinasebiology for your complete guide to kinase products and services
from Invitrogen.
50
Notes
51
www.invitrogen.com
MA
PR KD
The Adapta® assay utilizes Transcreener® HTS Assay Platform technology (7332278) under license from BellBrook Labs, LLC.
www.invitrogen.com
For research use only. Not intended for any animal or human therapeutic or diagnostic use, unless otherwise stated.
© 2010 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. Transcreener is a registered trademark of BellBrook Labs, LLC. Synergy™ 4,
Synergy™ 2, Synergy™ HT, and FLx800™ are trademarks of Biotek Instruments. Analyst®, FlexStation®, and SpectraMax® are registered trademarks of MDS Analytical Technologies. EnVision® is a registered trademark and VICTOR™ and ViewLux™ are trademarks of PerkinElmer. Infinite® is a registered trademark and Safire2™ is a trademark of Tecan Group Ltd. Varioskan® is a registered trademark of Thermo Scientific. PARADIGM™ is a trademark of Beckman Coulter. These products may be covered by one or
more Limited Use Label Licenses (see Invitrogen catalog or www.invitrogen.com). By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. CO11926 0410