Writing a Scientific Paper Adapted from Cox (1990) http://biology.luther.edu/paper.htm Although reviewing the scientific literature and writing a research paper is hard work, if well done the experience may be one of the most satisfying accomplishments that you have as a biology student. A well written scientific paper must fulfill two objectives. First, it must clearly and completely describe the procedures that were followed and the results that were obtained. Second, it must place these results in perspective by relating them to the existing state of knowledge and by interpreting their significance for future study. To place your research in perspective requires: 1. 2. 3. 4. summarizing the state of knowledge on the general topic relating your work to general body of knowledge on the topic stating the critical hypotheses toward which the study is addressed interpreting the results of the study in relation to these hypotheses and to the general state of knowledge 5. identifying the scientific questions and procedural weaknesses that need to be addressed in the future. While you need to do all of this, you also need to write clearly and as concisely as possible. Superfluous verbiage is a hindrance to the reader. Some examples of unclear and excessive writing include "The rats, numbering six, were...", "It can be noted that fewer...", or "The tapeworm, when in adulthood, can,...". These should simply be "Six rats were...", "Fewer...", or "The adult tapeworm can....". When editing, read your paper out loud, making sure your grammar is complete and your statements make sense! PAPER FORMAT Scientific papers usually include the following major sections, each clearly labeled: 1) Introduction 2) Procedure (or Materials and Methods) 3) Results 4) Discussion 5) Acknowledgments 6) Literature Cited This is not an absolute structure as some papers will be variations of this model. A brief outline of each section is given below. Title: Use the fewest possible words to adequately describe the contents of the paper. Introduction: explain exactly what the objectives of the study are, and why it's a worthwhile effort. In the end you should be able to defend each sentence if I ask "why did you include this information?" The introduction should address the following questions: 1 1) why did you undertake this study? 2) what is the state of existing knowledge? 3) what specifically are you going to do (clearly state your hypotheses and/or objectives) Procedure or Materials and Methods: answers all basic questions about how the study was done so that another scientist could repeat your experiment. Basically answer the questions where, when, and how. Be as specific and as concise as possible. I don't need to know you used a #2 pencil, but I do need to know if (for example) you made observations of some animal behavior, or where you obtained your test organisms. Be careful with terms. If you say you randomly sampled a site, make sure it was, in fact, a random sample and not a subjectively selected sample. Any statistical methods used to analyze your data should also be described here, citing a source for the use of the statistical tests, perhaps a statistics book. Results: should clearly describe what was found, and not require the reader to interpret data from figures and tables. Generally, results include only summarized data and observations obtained in the study. Do not present raw data. If you want to include actual data sheets these are an appendix to the paper. In addition, all figures and tables included in your paper must be cited in the results description. Most importantly, the results section should be free of interpretation of the data. If a figure can be used to show the data, use the figure (e.g. a graph) instead of a table. Most people understand graphs more quickly than tables. Don't present the same data in several ways, choose the one best way. While tables are good for presenting some kinds of data, consider the options. Do not present tables in text form! Both tables and figures should be clearly labeled Figure 1 or Table 1 in order of being referred to in the text, and should include a descriptive caption so they "stand alone" without needing reference to the text of the results section. Table captions are always above a table, while figure captions are placed below a graph, picture, or sketch. Each table or figure should be on a separate page, inserted in order immediately following the text page it is first cited on. Combine statements about the significance of differences examined by statistical tests with a precise indication of the test used and the probability level chosen. For example "the difference between the means was highly significant (t-test; t = 4.5; d.f. = 12; P < 0.01). Means (averages) should always be followed by an indication of the variation around that mean (25.4 ± 2.7, n=5), and the number of data points used to determine the mean should also be included. Discussion: is the interpretation of the data in relation to the original objectives or hypotheses. Relate your findings to the present state of knowledge and future needs for research. Make sure this is genuinely interpretive, not just a restatement of the introduction or results sections. In your discussion, address the following: 1) reach conclusions about the initial hypotheses and/or objectives 2) compare how your conclusions agree or contrast with previously published works 3) identify sources of error and inadequacies of your research 4) speculate upon broader meaning of the conclusions 2 5) identify needed next steps in research on the problem Acknowledgments: (optional, depending on the study) give credit to those who helped in your research through advice, work, permission, technical advice, monetary support, etc. Literature Cited: contains, in alphabetical order, only those items specifically referred to within the text. Items you read but did not specifically cite in the text of your paper should not be included. The following format should be used: To cite a journal article with one author: Howe, H.F. 1995. Succession and fire season in experimental prairie plantings. Ecology 76: 19171925. (this is the author, date, title of an article in the journal Ecology. The volume is 76 and the page numbers 1917-1925.) with two authors: Larsen, K.J. & R.E. Lee. 1994. Cold tolerance including rapid cold-hardening and inoculative freezing of all migrant Monarch butterflies in Ohio. Journal of Insect Physiology 40: 859-864. with three (or more) authors: Larsen, K.J., S.E. Heady & L.R. Nault. 1992. Influence of ants (Hymenoptera: Formicidae) on honeydew excretion and escape behaviors in a myrmecophile, Dalbulus quinquenotatus (Homoptera: Cicadellidae), and its congeners. Journal of Insect Behavior 5: 109-122. To cite a book: Southwood, T.R.E. 1984. Ecological Methods with Particular Reference to the Study of Insect Populations, second edition. Chapman & Hall, New York. To cite an article in an edited volume (book): Loreau, M. 1986. Niche differentiation and community organization in forest carabid beetles, pp. 465-487. In: Carabid Beetles, their Adaptations and Dynamics,, P.J. den Boer, M. Luff, D. Mossakowski & F. Weber (eds). Gustav Fisher, Stuttgart, Germany. Text citations: In the text of your paper, do not use footnotes or quotes. Instead, cite relevant information from the literature in your own words and acknowledge the source using author and date format as shown in the following examples: As discussed by Howe (1995), few projects studying the effect of fire..... Wet monarchs are significantly more susceptible to rapid freezing than dry monarchs (Larsen & Lee 1994). 3 If there are more than two authors of a source, cite the reference by the first author name and et al. along with the date. For example: (Larsen et al. 1992) or Larsen et al. (1992) for information from the paper on leafhopper behavior listed above written by Larsen, Heady & Nault in 1992. FINAL THOUGHTS If you're unsure how your paper should be assembled, ask your instructor! Be sure to double space, number all pages, and use a single staple in the upper left-hand corner. Scientific writing involves lots of peer review and constructive criticism. The attached scientific paper evaluation form will be used to help determine your grade for this project. Take red marks as opportunities, not personal attacks. Therefore, an opportunity may be given on this assignment to rewrite your paper if you so desire and the final grade for the assignment will be an average of the two grades. Bibliography Cox, G.W. 1990. Laboratory Manual of General Ecology, sixth edition. Wm. C. Brown Publishers, Dubuque, Iowa Name _______________________________ Scientific Paper Evaluation Form Score (2 = always, 1 = sometimes, 0 = rarely or never) Score Title: Comments descriptive and appropriate Introduction: sufficient background information so purpose of study is clear? hypothesis and/or objective(s) of study clearly stated Procedure or Materials and Methods: are methods clearly described? research design is appropriate Results: clear text description of results 4 figures and/or tables referred to in text figures and/or tables are essential, proper format, and "stand alone" with clear captions all relevant data is presented? are results statistical methods? adequately analyzed by appropriate Discussion: all relevant data discussed? data justifies conclusions/interpretations? facts, calculations, and interpretations correct? interpretations/conclusions compared with published works? Literature Cited: sufficient and appropriate (related) for scope of study? correct citation methodology in body of text (author date format)? correct bibliographic format? Overall: report is clearly written and logically organized? understanding of content clearly demonstrated by author? spelling and grammar OK, easy to read? Total Grading Score: ___/40 = ___% 5 6