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Supplemental Figure Legend
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Figure S1 (related to Figure 3).
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Gallery display of rich islet sympathetic innervation in young obese db/db mice. Three
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channels of fluorescence signals are presented in parallel to illustrate the islet microstructure,
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vasculature, and innervation in an integrated fashion. White: TH+ sympathetic nerves and
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endocrine cells. Red: blood vessels. Green/blue: nuclear signals and autofluorescence.
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Supplemental Movie Legends
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Movie S1 (related to Figures 1A-F).
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Whole-islet histology. A hyperplastic db/db islet is presented in the first half of the movie. The
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second half of the movie shows the 360° panoramic projection of the db/db islet sympathetic
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innervation. White: TH+ sympathetic nerves and endocrine cells. Red: blood vessels. Green:
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nuclear signals and autofluorescence of acini. Depth: 325 m. Specimen is immersed in the RI:
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1.52 solution.
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Movie S2 (related to Figures 1G-I).
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Matching the transmitted light and fluorescence signals in islet characterization. This movie
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presents the middle section of a hyperplastic ob/ob islet. Pancreatic specimen is immersed in the
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RI: 1.46 solution to improve the contrast in transmitted light microscopy. The islet and the
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surrounding pancreatic structures are recorded with matched transmitted light and fluorescence
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signals to verify the immunohistochemistry. White: TH+ sympathetic nerves and endocrine cells.
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Red: blood vessels. Green: nuclei. Depth: 100 m.
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Movie S3 (related to Figures 2D-I).
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Two examples of the peri- and intra-islet ducts in db/db pancreas. Both the large,
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hyperplastic islet (>300 m; the first half of the movie) and the medium islet (~100 m; second
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half) are identified with the ingrowth of ductal epithelium. Green: ductal marker CK19. Red:
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blood vessels. Blue: nuclear signals and autofluorescence of acini.
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Movie S4 (related to Figures 3A-C and G-J).
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Peri-ductal sympathetic innervation of the islet-duct complex in obesity. Two db/db islets
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related to Figures 3A-C and 3G-H were recorded in the first two-thirds of the movie. The last
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third of the movie shows the sympathetic innervation of the control islet in the db/+ lean
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littermate (Figures 3I-J). In the control (db/+ lean littermate), the nuclear signals on the left side
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of islet indicate a nearby duct around the arteriole(s). Prominent peri-ductal sympathetic
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innervation can be seen but the nerves are not directly associated with the islet. White: TH+
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sympathetic nerves and endocrine cells. Red: blood vessels. Cyan: nuclear signals and
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autofluorescence of acini.
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Movie S5 (related to Figures 3D-F).
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The neuro-insular-ductal complex. This movie shows the in-depth recording and 360°
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panoramic projection of the neuro-insular-ductal complex in the obese db/db pancreas. Abundant
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TH+ sympathetic axons and varicosities are seen concentrating at the islet-duct boundary,
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forming the unique complex. Blue: insulin. White: TH. Red: blood vessels.
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Movie S6 (related to Figures 6M-O).
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Pericyte remodeling in the ob/ob islet. First half: tile scanning and in-depth projection of the
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large, hyperplastic islet presented in Figure 6M. Second half: 360° projection of the NG2 signals
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to reveal the drastic difference between the pericytes in the islet and that of the acini. The former
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becomes hypertrophic with circular expansion of the processes to enclose the microvessels; the
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latter stays intact with their processes extending toward the longitudinal direction of the
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capillary. Green: pericyte surface marker NG2. Red: blood vessels. Blue: nuclear signals and
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autofluorescence of acini.
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