Frequencies & Percentages
of Mouse Immune Cell Types
1,2
CELL TYPE
TISSUE SOURCE
STEMCELL TECHNOLOGIES
CELL ISOLATION KITS 4 (CATALOG #)
FREQUENCIES OR PERCENTAGES
(cells/µL of blood or % of total cells)3
SELECTION METHOD
NEGATIVE 5
POSITIVE 6
Erythrocytes
Peripheral Blood
10.6 ± 0.4 x 103
­—
­­—
Platelets
Peripheral Blood
1157 ± 123 x 103
­­—
­—
Leukocytes
Peripheral Blood
2.6 ± 0.9 x 103
­—
­—
Lymphocytes
Peripheral Blood
2.0 ± 0.8 x 103
­­—
­—
T Cells
Spleen
21.3 ± 5.3%
19851
18951 (CD90.2+)
Naïve T Cells
Spleen
21.3 ± 4.9%
19848
­—
CD4+ T Cells
Spleen
13.6 ± 1.5%
19852
18952 (CD4+)
Naïve CD4+ T Cells
Spleen
12.6 ± 1.9%
19765
18765 (CD4+CD62L+)
Memory CD4+ T Cells
Spleen
6.2 ± 1.7%
19767
­—
Regulatory T Cells (CD4+CD25+)
Spleen
2.0 ± 0.3%
­—
19782 (CD4+CD25+)
CD8+ T Cells
Spleen
8.6 ± 0.7%
19853
18953 (CD8a+)
Naïve CD8+ T Cells
Spleen
12.0 ± 2.4%
19858
­—
Memory CD8+ T Cells
Spleen
3.4 ± 1.0%
­—
­—
NK T Cells
Spleen
1.9 ± 0.6%
­—
­—
19854
18954 (CD19+)
198447
­—
19855
18755 (CD49b+)
B Cells
Spleen
57.8 ± 7.2%
NK Cells
Spleen
1.1 ± 0.2%
Spleen
4.2 ± 0.7%
Bone Marrow
13.2 ± 2.8%
Peripheral Blood
0.04 ± 0.03 x 10
Pan-Dendritic Cells
Spleen
4.7 ± 2.0%
19763
­­18780 (CD11c+)
Conventional Dendritic Cells
Spleen
4.1 ± 1.7%
­—
­—
Plasmacytoid Dendritic Cells
Spleen
0.4 ± 0.2%
19764
—
Granulocytes
Spleen
1.3 ± 1.4%
—
­—
Bone Marrow
34.0 ± 9.0%
Peripheral Blood
0.5 ± 0.5 x 10
19762
­—
Eosinophils
Peripheral Blood
0.06 ± 0.05 x 103
­—
­—
Basophils
Peripheral Blood
0.005 ± 0.006 x 103
­—
­—
Monocytes
Neutrophils
18770 (CD11b+)
19861
­—
3
3
18757 (CD117+)
Hematopoietic Progenitor Cells
(Lin−c-Kit+)
19856
Bone Marrow
18762 (CD93+)
0.17 ± 0.03%
­—
18756 (Sca1+)
18856 (Sca1+)
1. Cell type frequencies and percentages are for 6 to 12 weeks old C57BL/6 or C57BL/6J mice. The values will vary depending on mouse strain, age, gender, and environment. 2. Data sourced from: A. The Jackson Laboratory. Flow-cytometric analysis of 11 inbred strains of mice.
MPD:Jaxpheno6. Mouse Phenome Database website, The Jackson Laboratory, Bar Harbor, Maine, USA. http://phenome.jax.org, Aug, 2013; B. The Jackson Laboratory. Hematological survey of 11 inbred strains of mice. MPD:Jaxpheno4. Mouse Phenome Database website, The Jackson Laboratory,
Bar Harbor, Maine, USA. http://phenome.jax.org, Aug, 2013; C. STEMCELL Technologies’ RND Scientists. 3. Peripheral blood values expressed as cells/µL of blood. Spleen and bone marrow values expressed as % of total splenocytes or bone marrow cells. 4. EasySep™/RoboSep™ Immunomagnetic
Isolation. 5. Negative selection labels unwanted cells to isolate desired untouched cells. 6. Positive selection labels and isolates desired cell types. 7. Without CD43 depletion; for pan-B cells.
Isolate Cells In As Little As 15 Minutes
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