Biotechnology Applications
Reading Assignments
Benchmarks:
 Information is stored, processed and can be altered.
 Techniques are designed for identification, enhancement and/or treatment.
Reading Assignments are in: Biotechnology (BT) only
Topic / Key Vocabulary
How can we manipulate organisms?
enhancement, treatment
prokaryotic, eukaryotic, nucleus, nucleolus, golgi, endoplasmic reticulum,
cytoplasm, cell membrane, cell wall, ribosomes, mitochondria, vacuole,
lysosome
carbohydrate, Lipid, Nucleic Acid, Protein, Covalent bond, Hydrogen bond,
monosaccaride, fatty acid, glycerol, amino acid, nucleic acid
How can we utilize DNA for identification, enhancement and treatment?
DNA, nucleotide, purine, pyrimidine, adenine, cytosine, thymine, guanine,
backbone, deoxyribose, phosphate, hydrogen bond, covalent bond, nitrogenous
base
blunt, sticky, palindrome, restriction enzyme, gel electrophoresis, buffer,
centrifuge
replication, template, in-vivo, in-vitro, DNA polymerase, primer, leading strand,
lagging strand, okazaki fragment, nucleotide, replication fork, replication bubble,
3’-end, 5’-end, PCR, sequencing, amplification, terminator, dNTP, ddNTP, cycle,
denature, extension, anneal, genomic DNA, plasmid spectrophotometer, OD,
SNP, mutation, silent, expressed
transformation, plasmid, origin of replication, selection gene, multiple cloning
sites, promoter, restriction enzyme, blunt, sticky, competent cell, selection,
positive control, negative control, vector, retrovirus, efficiency, GMO
Reading
Assignment
Read & Take
Notes On
pages
Do Section
Review
Assignment 1 37-47
2.1, 2.2
Assignment 2 48-57
2.3
Assignment 3 99-103
Assignment 4 116-121
4.1
4.4
Assignment 5 343-348
Assignment 6 209-214
Assignment 7 226-233
Assignment 8 349-353
Assignment 9 354-360
Assignment 10 369-374
13.1
8.1
8.4 # 2 only,
and 8.5
13.2
13.3, 13.4
14.1
Assignment 11 215-222
8.2
STANDARD 5 Students will describe the structure and function of cells and their components.
OBJECTIVE 1: Identify key cellular components and correlate with function. (i.e., nucleus, chromosomes, ribosomes)
a.
Describe the structure of nucleus, nucleolus, endoplasmic reticulum, golgi apparatus, ribosomes, mitochondria, etc.
b.
Explain the major function of each.
OBJECTIVE 2: Compare and contrast prokaryotic and eukaryotic cells.
a. Describe a prokaryotic cell: example – cell size, cell wall, cell membrane, genetic material,etc.
b. Describe a eukaryotic cell: example – cell size, cell membrane, genetic material, membrane bound organelles, etc.
STANDARD 7 Students will compare and contrast different types of nucleic acids and proteins and illustrate the flow of genetic information within the cell.
OBJECTIVE 1: Describe the structure of nucleic acids.
a.
Identify the components of nucleotides.
b.
Compare and contrast the structure and function of DNA and RNA.
c.
Explain how the chemical structure of DNA applies to gel electrophoresis.
d.
Perform a restriction digest and analyze the results with gel electrophoresis.
OBJECTIVE 2: Describe how DNA functions as a template for DNA replication.
a.
Identify the major components and outline the process of DNA replication.
b.
Explain how DNA replication applies to the amplification of nucleic acids in PCR and DNA sequencing.
c.
Amplify and analyze DNA using PCR and gel electrophoresis.
d.
Demonstrate the ability to use PCR technology.
STANDARD 8 Students will explain recombinant DNA techniques in bacteria.
OBJECTIVE 1: Describe the use of plasmids in bacterial transformation.
a.
Describe the elements of a functional plasmid (origin of replication, selection gene, multiple cloning sites, and promoter).
b.
Explain the role of restriction enzymes in generating recombinant plasmids.
c.
Describe competent cells, transformation and selection methods.
d.
Perform a bacterial transformation and analyze results.
OBJECTIVE 2: Describe the process of plasmid DNA isolation.
a.
Analyze the protocol for isolating plasmid DNA.
b.
Understand how to quantify the amount of DNA purified.