Last data updates: 06 January 2011 Tian, J.H. and Han, J.S. (2000), Functional studies using antibodies against orphanin FQ/nociceptin. Peptides, 21 (7), 1047-1050. Document type: Article Language: English Cited references: 20 Time cited: 15 Times self cited: 1 Abstract: Orphanin FQ/nociceptin (OFQ) is a recently discovered endogenous ligand for the novel opioid receptor-like receptor (ORL-1). There are numerous reports in the literature demonstrating paradoxical effects of exogenous OFQ on pain modulation. For example, OFQ produces a pronociceptive effect in the brain and an analgesic effect in the spinal cord. In order to better understand the physiological actions of OFQ, the present study focused on the pain-modulatory effect of endogenously released OFQ measured using antibody microinjection techniques. We found that electroacupuncture analgesia (EA) was increased by intracerebroventricular (i.c.v.) injection of an OFQ;antibody and decreased following intrathecal injection. Furthermore, i.c.v. OFQ-antibody partially reversed tolerance to both chronic morphine and chronic EA. These data suggest that endogenously released OFQ plays an important role in pain modulation, where pain sensitivity in the brain and spinal cord is increased and decreased, respectively. (C) 2000 Elsevier Science Inc. All rights reserved. Author Keywords: orphanin FQ; nociceptin; antibody; pain; tolerance keywords plus: anti-opioid peptide; rat spinal-cord; nociceptin receptor; induced analgesia; electroacupuncture analgesia; periaqueductal gray; in-vivo; fq; agonist; <phe(1)psi(ch2-nh)gly(2)>nociceptin-(1-13)-nh2 Reprint Address: Han, JS (reprint author), Beijing Med Univ, Neurosci Res Inst, Beijing 100083, Peoples R China Addresses: 1. Beijing Med Univ, Neurosci Res Inst, Beijing 100083, Peoples R China 1. 2. Parenti, C. and Scoto, G.M. 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