The following is a list of references we find to be particularly interesting. These
references are provided as a guide to the various uses our customers are making of our
products. The methods referenced here provide a starting point may not be optimized for
all situations. Some empirical experimentation may be required. At the end of the list is
a searchable database for additional citations.
---------------------------------------------------Aicher A, Heeschen C, Mildner-Rihm C, Urbich C, Ihling C, Technau-Ihling K, Zeiher
AM, Dimmeler S. (2003). Essential Role of Endothelial Nitric Oxide Synthase for
Mobilization of Stem and Progenitor Cells. Nature Medicine 9:1370-1376. Abstract
Invitrogen products used: CellTracker Orange
Amit M, Shariki C, Margulets V, Itskovitz-Eldor J. (2003). Feeder Layer- and SerumFree Culture of Human Embryonic Stem Cells. Biology of Reproduction 70:837-845.
Abstract
Invitrogen products used: KnockOut D-MEM, KnockOut Serum Replacement, Lglutamine, 2-mercaptoethanol, MEM Non-Essential Amino Acid Solution, basic
fibroblast growth factor, collagenase IV
Blyszczuk P, Czyz J, Kania G, Wagner M, Roll U, St-Onge L, Wobus AM. (2003).
Expression of Pax4 in Embryonic Stem Cells Promotes Differentiation of Nestin-positive
Progenitor and Insulin-producing Cells. Proc. Natl. Acad. Sci. U.S.A.100:998-1003.
Abstract
Invitrogen products used: IMDM (Iscoves Modified Dulbecco’s Medium), trypsin,
transferrin, fibronectin, B-27
Dong X, Pulido JS, Qu T, Sugaya K. (2003) Differentiation of Human Neural Stem Cells
into Retinal Cells. Neuroreport 14:143-146. Abstract
Invitrogen products used: D-MEM/F-12, B-27
Goolsby J, Marty MC, Heletz D, Chiappelli J, Tashko G, Yarnell D, Fishman PS, DhibJalbut S, Bever CT Jr, Pessac B, Trisler D. (2003) Hematopoietic Progenitors Express
Neural Genes. Proc. Natl. Acad. Sci. U.S.A.100:14926-14931. Abstract
Invitrogen products used: D-MEM, CD Hybridoma, HuC/HuD antibody, CellTracker
Orange.
Horii T,Nagao Y, Tokunaga T, Imai H. (2003). Serum-free Culture of Murine Primordial
Germ Cells and Embryonic Germ Cells. Theriogenology 59:1257-1264. Abstract
Invitrogen products used: KnockOut D-MEM, KnockOut Serum Replacement, ESQualified FBS.
Hung S-C, Chen NJ, Hsieh SL, Li H, Ma HL, Lo WH. (2002). Isolation and
Characterization of Size-Sieved Stem Cells from Human Bone Marrow. Stem Cells
20:249-258. Abstract
Invitrogen products used: D-MEM (low glucose), FBS, PBS
Hwang WS, Ryu YJ, Park JH, Park ES, Lee EG, Koo JM, Jeon HY, Lee BC, Kang SK,
Kim SJ, Ahn C, Hwang JH, Park KY, Cibelli JB, Moon SY. (2004). Evidence of a
Pluripotent Human Embryonic Stem Cell Line Derived from a Cloned Blastocyte.
Science E-publication ahead of print. Abstract
Invitrogen products used: DMEM, DMEM/F12, KnockOut Serum Replacement, bFGF
Jungling K, Nagler K, Pfrieger FW, Gottmann K. (2003). Purification of embryonic stem
cell-derived neurons by immunoisolation. FASEB Journal. 17:2100-2102. Abstract
Invitrogen products used: Neurobasal, B-27, GlutaMAX
Kawasaki H, Suemori H, Mizuseki K, Watanabe K, Urano F, Ichinose H, Haruta M,
Takahashi M, Yoshikawa K, Nishikawa S, Nakatsuji N, Sasai Y. (2002). Generation of
Dopaminergic Neurons and Pigmented Epithelia for Primate ES Cells by Stromal CellDerived Inducing Activity. Proc. Natl. Acad. Sci. U.S.A. 99:1580-1585. Abtract
Invitrogen products used: KnockOut Serum Replacement, FITC-phalloidin, YOYO-1
Li H, Roblin G, Liu H, Heller S. (2003). Generation of Hair Cells by Stepwise
Differentiation of Embryonic Stem Cells. Proc. Natl. Acad. Sci. U.S.A. 100:13495-13500.
Abstract
Invitrogen products used: KnockOut D-MEM, FCS, non-essential amino acids, 2mercaptoethanol, L-glutamine, SuperScript II Reverse Transcriptase
Lind EF, Prockop SE, Porritt HE, Petrie HT. (2001). Mapping Precusor Movement
Through the Postnatal Thymus Reveals Specific Microenvironments Supporting Defined
Stages of Early Lymphoid Development. J. Exp. Med. 194:127-134. Abstract.
Invitrogen products used: Vybrant CFDA-SE Cell Tracer Kit, Prolong anti-fade media
Liu S, Qu Y, Stewart TJ, Howard MJ, Chakrabortty S, Holekamp TF, McDonald JW.
(2000). Embryonic Stem Cells Differentiate into Oligodendrocytes and Myelinate in
Culture and After Spinal Cord Transplantation. Proc. Natl. Acad. Sci. U.S.A. 97:61266131. Abstract
Invitrogen products used:Cell Tracker Orange
Lowe B, Avila HA, Bloom FR, Gleeson M, Kusser W. (2003). Quantitation of Gene
Expression in Neural Precursors by Reverse Transcription Polymerase Chain Reaction
using Self-Quenched, Fluorogenic Primers. Analytical Biochemistry 315:95-105. Abstract
Invitrogen products used: LUX primers, -MEM, FBS, Neurobasal Medium, B-27, Lglutamine, Trizol, T7 RNA Polymerase, Topo Tools, Taq DNA Polymerase, Micro-toMidi Total RNA Purification System, SuperScript II Reverse Transcriptase, Platimun
Quantitative PCR SuperMix-UDG
Luo Y, Cai J, Ginis I, Sun Y, Lee S, Yu SX, Hoke A, Rao M. (2003). Designing, Testing,
and Validating a Focused Stem Cell Microarray for Characterization of Neural Stem
Cells and Progenitor Cells. Stem Cells 21:575-587. Abstract
Invitrogen products used: KnockOut D-MEM, ES-qualified FBS, non-essential amino
acids, 2-mercaptoethanol, L-glutamine, Trizol
Mangi AA, Noiseux N, Kong D, He H, Rezvani M, Ingwall JS, Dzau VJ. (2003).
Mesenchymal Stem Cells Modified with Akt Prevent Remodeling and Restore
Performance of Infarcted Hearts. Nature Medicine 9:1195-1201. Abstract
Invitrogen products used: MEM, GlutaMAX, FBS, antibiotics, TRIzol reagent, OneStep RT-PCR with Platinum Taq kit
Maurer MH., Feldmann RE Jr, Futterer CD, Kuschinsky W. (2003). The Proteome of
Neural Stem Cells from Adult Rat Hippocampus. Proteome Science 1:4. Abstract
Invitrogen products used: Neurobasal medium, B-27
Oswald J, Boxberger S, Jorgensen B, Feldmann S, Ehninger G, Bornhauser M, Werner C.
(2004). Mesenchymal Stem Cells Can Be Differentiated Into Endothelial Cells In Vitro.
Stem Cells 22:377-384. Abstract
Invitrogen products used: D-MEM-Low Glucose, GlutaMAX-I
Prudhomme W, Daley GQ, Zandstra P, Lauffenburger DA. (2004). Multivariate
Proteomic Analysis of Murine Embryonic Stem Cell Self-renewal Versus Differentiation
Signaling. Proc. Natl. Acad. Sci. U.S.A.101:2900-2905. Abstract
Invitrogen products used: KnockOut Serum Replacement, Alexa Fluor 488
Richards M, Fong CY, Chan WK, Wong PC, Bongso A. (2002). Human Feeders Support
Prolonged Undifferentiated Growth of Human Inner Cell Masses and Embryonic Stem
Cells. Nature Biotechnology 20:933-936. Abstract
Invitrogen products used: KnockOut D-MEM, D-MEM, glutamine, penicillinstreptomycin, nonessential amino acids solution, insulin-transferrin-selenium supplement
Richards M, Tan SP, Tan JH, Chan WK, Bongso A. (2004). The Transriptome Profile of
Human Embryonic Stem Cells as Defined by SAGE. Stem Cells 22:51-64. Abstract
Invitrogen products used: TRIzol, I-SAGE kit, One Shot Top 10 E. coli, SAGE2000
software
Romanov YA, Svintsitskaya VA, Smirnov VN. (2003). Searching for Alternative Sources
of Postnatal Human Mesenchymal Stem Cells: Candidate MSC-Like Cells from
Umbilical Cord. Stem Cell 21:105-110. Abstract
Invitrogen products used: D-MEM (low glucose), Medium 199, D-PBS, EBSS,
penicillin-strepomycin, L-glutamine, sodium pyruvate, trypsin-EDTA
Romero-Ramos M, Vourc'h P, Young HE, Lucas PA, Wu Y, Chivatakarn O, Zaman R,
Dunkelman N, el-Kalay MA, Chesselet MF. (2002). Neuronal differentiation of stem
cells isolated from adult muscle. J.Neurosci.Res. 69:894-907. Abstract
Invitrogen products used: Neurobasal A, B-27, N2
Sato N, Meijer L, Skaltsounis L, Greengard P, Brivanlou AH. (2004). Maintenance of
Pluripotency in Human and Mouse Embryonic Stem Cells through Activation of Wnt
Signaling by a Pharmacological GSK-3-specific Inhibitor. Nature Medicine 10:55-63.
Abstract
Invitrogen products used: D-MEM/F12, KnockOut Serum Replacement, L-glutamine,
MEM Nonessential amino acids, 2-mercaptoethanol, basic fibroblast growth factor
Schuldiner, M, Yanuka O, Itskovitz-Eldor J, Melton DA, Benvenisty N. (2000). Effects
of Eight Growth Factors on the Differentiation of Cells Derived from Human Embryonic
Stem Cells. Proc. Natl. Acad. Sci. U.S.A. 97:11307-11312. Abstract
Invitrogen products used: KnockOut D-MEM, KnockOut Serum Replacement, MEM
Non-essential Amino Acids, L-glutamine, basic FGF, trypsin
Schuldiner M, Itskovitz-Eldor J. Benvenisty N. (2003). Selective Ablation of Human
Embryonic Stem Cells Expressing a “Suicide” Gene. Stem Cells 21: 257-265. Abstract
Invitrogen products used: KnockOut SR, KnockOut D-MEM, L-glutamine, MEM
Non-essential amino acids, bFGF, MMLV Reverse Transcriptase
Schulz TC, Palmarini GM, Noggle SA, Weiler DA, Mitalipova MM, Condie BG. (2003).
Directed Neuronal Differentiation of Human Embryonic Stem Cells. BMC Neuroscience
4:27. Abstract
Invitrogen products used: DMEM/F12, KnockOut Serum Replacement, MEM nonessential amino acids, penicillin-streptomycin, L-glutamine, Alexa-350, Alexa-488 and
Alexa –568 conjugated antibodies
Segev H, Fishman B, Ziskind A, Shulman M, Itskovitz-Eldor J. (2004). Differentiation of
Human Embryonic Stem Cells into Insulin-Producing Clusters. Stem Cells 22:265-274.
Abstract
Invitrogen products used: KnockOut D-MEM, KnockOut Serum Replacement, MEM
Non-essential Amino Acids, L-glutamine, basic FGF, collagenase IV, D-MEM/F12, N2,
B-27, insulin-transferrin-selenium, TO-PRO-3 iodide
Sowa H, Kaji H, Canaff L, Hendy GN, Tsukamoto T, Yamaguchi T, Miyazono K,
Sugimoto T, Chihara K. (2003) Inactivation of Menin, the Product of the Multiple
Endocrine Neoplasia Type 1 Gene, Inhibits the Commitment of Multipotential
Mesenchymal Stem Cells into the Osteoblast Lineage, JBC 278:21058-21069. Abstract
Invitrogen products used: Eagle’s basal medium, FBS, penicillin-streptomycin, MEM.
D-MEM, G418, lipofectamine, SuperScript first strand synthesis system for RT-PCR
Spees JL, Olson SD, Ylostalo J, Lynch PJ, Smith J, Perry A, Peister A, Wang MY,
Prockop DJ. (2003). Differentiation, Cell Fusion, and Nuclear Fusion During ex vivo
Repair of Epithelium by Human Adult Stem Cells for Bone Marrow Stroma. Proc. Natl.
Acad. Sci. U.S.A. 100:2397-2402. Abstract
Invitrogen products used: CellTracker Green, MEM, L-glutamine, penicillinstreptomycin
Sperger JM, Chen X, Draper JS, Antosiewicz JE, Chon CH, Jones SB, Brooks JD,
Andrews PW, Brown PO, Thomson JA. (2003). Gene Expression Patterns in Human
Embryonic Stem Cells and Human Pluripotent Germ Cell Tumors. Proc. Natl. Acad. Sci.
U.S.A.100:13350-13355. Abstract
Invitrogen products used: D-MEM, FCS, L-glutamine, trypsin-EDTA, MEM
Nonessential amino acids, dispase, collagenase IV
Vallier L, Rugg-Gunn PJ, Bouhon IA, Andersson FK, Sadler AJ, Pedersen RA. (2004).
Enhancing and Diminishing Gene Function in Human Embryonic Stem Cells. Stem Cells
22:2-11. Abstract
Invitrogen products used: KnockOut D-MEM, KnockOut Serum Replacement,
Collagenase IV, Lipofectamine 2000, Oligofectamine
Wachs F-P, Couillard-Despres S, Engelhardt M, Wilhelm D, Ploetz S, Vroemen M,
Kaesbauer J, Uyanik G, Klucken J, Karl C, Tebbing J, Svendsen C, Weidner N, Kuhn
HG, Winkler J, Aigner L. (2003). High Efficacy of Clonal Growth and Expansion of
Adult Neural Stem Cells. Laboratory Investigation 83:949-962. Abstract
Invitrogen products used: Neurobasal medium, B-27
Ward CM, Stern P, Willington MA, Flenniken AM. (2002) Efficient Germline
Transmission of Mouse Embryonic Stem Cells Grown in Synthetic Serum in the Absence
of a Fibroblast Feeder Layer. Laboratory Investigation 82:1765-1767 Abstract
Invitrogen products used: KnockOut SR, KnockOut D-MEM, L-glutamine, 2mercaptoethanol, MEM Non-essential amino acids.
Ward CM, Stern PL (2002) The Human Cytomegalovirus Immediate-Early Promoter is
Transcriptionally Active in Undifferentiated Mouse Embryonic Stem Cells. Stem Cells
20:472-475 Abstract
Invitrogen products used: KnockOut SR, KnockOut D-MEM, 2-mercaptoethanol,
TOP10F competent cells, Lipofectamine Reagent
Wislet-Gendebien S, Leprince P, Moonen G, Rogister B. (2003). Regulation of Neural
Markers Nestin and GFAP Expression by Cultivated Bone Marrow Stromal Cells.
Journal of Cell Science 116:3295-3302. Abstract
Invitrogen products used: DiD Vybrant cell-labeling solution, D-MEM, D-MEM/F12,
FBS, N2, B-27, Neurobasal Medium
Wu P, Tarasenko YI, Gu Y, Huang LY, Coggeshall RE, Yu Y. (2002). Region-specific
Generation of Cholinergic Neurons from Fetal Human Neural Stem Cells Grafted in
Adult Rat. NatureNeuroscience 5:1271-1278. Abstract
Invitrogen products used: D-MEM/F-12, N2
Xu C, Police S, Rao N, Carpenter MK. (2002). Characterization and Enrichment of
Cardiomyocytes Derived from Human Embryonic Stem Cells. Circulation Research
91:501-508. Abstract
Invitrogen products used: KnockOut D-MEM, collagenase IV, non-essential amino
acids, -mercaptoethanol, L-glutamine
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