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BIOCARTA Europe GmbH
Borsteler Chaussee 53, D-22453 Hamburg Germany
Tel +49-40-5257030 Fax +49-40-52570377
info.de@biocarta.com
CD10 (CALLA)
Concentrated Monoclonal Antibody
Cat. #CM129A: 0.1 ml, Cat. #CM129B: 0.5 ml, Cat. #CM129C: 1.0 ml
Specificity and Comments
Human CD10, also known as common acute lymphoblastic leukemia
(CALLA), has been shown to react with TdT+ lymphoblastic
leukemia, follicular germinal cell lymphoma, Burkitt’s lymphoma,
and chronic myelocytic leukemia. CD10 also marks normal early
lymphoid progenitor cells, immature B-cells in adult bone marrow,
and germinal cells in normal tonsil and/or normal lymphoid tissue. It
is also expressed in some non-lymphoid tissues such as fibroblasts,
breast myoepithelium, and brush border of kidney. Recently, CD10
has been used in a panel for mantle cell lymphoma with cyclin D1(+),
CD43 (+), CD5 (+), IgM (+), CD23 () and CD10 ().
ReVeal maybe substituted, but will require longer incubation times
with the CD10 primary antibody. Allow the solution to cool for 20
minutes.
Alternatively, steam tissue sections for 45-60 minutes, or use a
pressure cooker for 2-3 minutes
(BIOCARE’S Decloaking
Chamber). Cool for 10 minutes.
Protein Serum Block (Optional)
Incubate for 5 minutes at room temperature (RT).
Primary Antibody
Species Reactivity: Human. Other not tested.
Dilute 1:25-50. Incubate for 30-60 minutes
Clone: 56C6
Biotinylated Secondary Antibody
Isotype: IgG1
Incubate for 10 minutes at RT with BIOCARE’S 4plus Detection
System.
Immunogen:
Enzyme Labeled Streptavidin
Prokaryotic recombinant fusion protein corresponding to the external
of the CD10 glycoprotein.
Incubate for 10 minutes at RT with BIOCARE’S 4plus Detection
System.
Cellular Location: Membrane
Chromogen
Control Tissue:
Incubate for 5 minutes at RT with BIOCARE’S
CHROMOGENCARE.
Tonsil, kidney, lymphoblastic leukemia
Technical Note
Known Applications:
CD10 is a cell surface marker that may show low expression in
certain tumors and/or tissues. Overstaining with hematoxylin can
mask staining, thus reducing overall contrast and making
interpretation difficult. It is recommended that a light hematoxylin
stain be applied (15 to 30 seconds with Tacha’s Hematoxylin). If
using high pH retrieval solutions, some tissues may require an avidinbiotin block.
Frozen and formalin-fixed paraffin-embedded tissues
Supplied As:
Culture supernatant with preservative
Storage and Stability:
Store vial at 4°C. Stable for 18 months.
Protocol Notes
Protocol Recommendations:
The optimum antibody dilution and protocols for a specific
application can vary due to many factors. These include, but are not
limited to: fixation, incubation times, tissue section thickness and
detection kit used. The data sheet’s recommendations and protocols
are based on exclusive use of BIOCARE products. Due to the
superior sensitivity of these unique reagents, the recommended
incubation times and titers listed are not applicable to other detection
systems, as results may vary. Ultimately, it is the responsibility of
the investigator to determine optimal conditions.
Endogenous Peroxidase Block
If using an HRP system, block for 5 minutes with BIOCARE’S
PEROXIDAZED 1.
Pretreatment (Required)
Gently boil tissue sections
NUCLEARDECLOAKER.
(100C)
for
20
minutes
For Research use only. Not for therapeutic or in vitro diagnostic use.
Revised 2/13/16
in
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Limitations and Warranty
This antibody is available for research use only. Not approved for
use in humans or in clinical diagnosis. There are no warranties,
expressed or implied, which extend beyond this description.
BIOCARE is not liable for property damage, personal injury, or
economic loss caused by this product.
References:
1.
2.
3.
4.
Kaufmann O, Flath B, Spath-Schwalbe E, Possinger K, Dietel
M. Immunohistochemical detection of CD10 with monoclonal
antibody 56C6 on paraffin sections. Am J Clin Pathol 1999
Jan;111(1):117-22 .
Kurtin PJ, Hobday KS, Ziesmer S, Caron BL. Demonstration of
distinct antigenic profiles of small B-cell lymphomas by paraffin
section immunohistochemistry.
Am J Clin Pathol 1999
Sep;112(3):319-29.
de Leon ED, Alkan S, Huang JC, Hsi ED. Usefulness of an
immunohistochemical panel in paraffin-embedded tissues for the
differentiation of B-cell non-Hodgkin's lymphomas of small
lymphocytes. Mod Pathol 1998 Nov;11(11):1046-51.
de Boer CJ, van Krieken JH, Schuuring E, KluinPM. Bcl1/cyclin D1 in malignant lymphoma. Ann Oncol 1997;8
Suppl 2:109-17.
BIOCARE Products
Catalog # Size
.
Product
Description
A one-step dewaxing, heat retrieval, biotin blocking
solution (color-coded with ph indicator)
10X tris buffer solution (pH 9.5) for heat retrieval methods
(color-coded with pH indicator)
RV1000M
500ml
10 X ReVeal
CB911M
500ml
NUCLEARDECLOAKER
HP504UR
50ml
4plus HRP 500 Universal
Universal Horseradish Peroxidase Detection System
AC800P
DB801R
25ml
50ml
AEC250 Chromogen Pack
DAB500 Chromogen Pack
AEC – Liquid, two-component
DAB – Liquid, two-component
DC2000
1 each
Decloaking Chamber
Electric Pressure Cooker for heat retrieval methods
(superior to microwave pressure cooking methods)
For Research use only. Not for therapeutic or in vitro diagnostic use.
Revised 2/13/16
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