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BIOCARTA Europe GmbH
Borsteler Chaussee 53, D-22453 Hamburg Germany
Tel +49-40-5257030 Fax +49-40-52570377
info.de@biocarta.com
CD31 (PECAM-1)
Concentrated Monoclonal Antibody
Cat: # CM131A: 0.1 ml, Cat: #CM131D: 0.25 ml, Cat: #CM131B: 0.5 ml
Specificity and Comments:
Second Post-Pretreatment (Optional)
CD31 recognizes a 100kDA glycoprotein in endothelial cells and
130kD in platelets. It reacts weakly with mantle zone B-cells,
peripheral T-cells, and neutrophils. CD31 can detect vascular
endothelium associated antigen and has been used as a marker for
benign and malignant human vascular disorders, myeloid leukemia
infiltrates and megakaryocytes in normal bone marrow. When
compared to Factor VIII and CD34 antibodies, studies have shown
CD31 to be a superior marker for angiogenesis. CD31 has been used
to measure angiogenesis, which reportedly predicts tumor recurrence.
CD31 used in a panel with CD34 and Factor VIII has also been used
to mark Kaposi’s sarcoma and angiosarcomas. Other studies have
also indicated that CD31 and CD34 can be used as markers for
myeloid progenitor cells that recognize different subsets of myeloid
leukemia infiltrates (granular sarcomas).
If tissues are overfixed, apply cold pepsin for 1 minute and wash in
DI water.
Species Reactivity: Human; other not tested.
Clone: JC/70A
Isotype: IgG1/
Known Applications:
Immunohistochemistry (frozen /formalin-fixed paraffin-embedded
tissues)
Positive Control: Tonsil or angiosarcoma
Cellular Localization:
Cytoplasmic and membrane
Supplied As:
PBS, pH 7.4 with carrier protein and preservative
Protein Block
Incubate for 5 minutes at room temperature (RT) with BIOCARE’S
BACKGROUNDERASER.
Primary Antibody
Dilute 1:25-1:50. Incubate for 30-120 minutes at RT.
Biotinylated Secondary Antibody
Incubate for 10-20 minutes at RT.
Enzyme Labeled Streptavidin
Incubate for 10-20 minutes at RT with BIOCARE’S 4plus
Detection System.
Chromogen
Incubate
for
5-10
minutes.
Use
BIOCARE’S
CHROMOGENCARE.
For additional enhancement, apply
BIOCARE’S DABSPARKLE for 1 minute.
Technical Notes
NUCLEARDECLOAKER is a potent agent for epitope retrieval
and tissues may be 4 to 10 times more sensitive to
immunohistochemical staining.
Certain tissues may express
nonspecific background staining due to endogenous biotin. Blocking
of endogenous biotin can be quenched by using BIOCARE’S AvidinBiotin Blocking Kit (Cat. #AB972G).
Endogenous Peroxidase Block
Positive-charged slides for tissue adhesion should be used for heatinduced epitope retrieval methods. Do not use adhesives in the water
bath. Dry slides at 25C overnight and then dry for 10-30 minutes at
60 C. Be sure that all water is removed from the tissue. Use
BIOCARE’S KLING-ON Slides and Desert Chamber Drying Oven.
If using an HRP system, block for 5 minutes with BIOCARE’S
PEROXIDAZED 1.
Protocol Notes
Protocol Recommendations:
Pretreatment (Required)
Gently boil tissue sections (100C) for 20 minutes in
NUCLEARDECLOAKER. Allow the solution to cool for 20 minutes.
Alternatively, steam tissue sections for 45-60 minutes, or use a
pressure cooker for 2-5 minutes
(BIOCARE’S Decloaking
Chamber).
For Research use only. Not for therapeutic or in vitro diagnostic use.
Revised 2/12/16
The optimum antibody dilution and protocols for a specific
application can vary due to many factors. These include, but are not
limited to: fixation, incubation times, tissue section thickness and
detection kit used. The data sheet’s recommendations and protocols
are based on exclusive use of BIOCARE products. Due to the
superior sensitivity of these unique reagents, the recommended
incubation times and titers listed are not applicable to other detection
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systems, as results may vary. Ultimately, it is the responsibility of
the investigator to determine optimal conditions.
Limitations and Warranty
This antibody is available for research use only. It is not approved
for use in humans or in clinical diagnosis. There are no warranties,
expressed or implied, which extend beyond this description.
BIOCARE is not liable for property damage, personal injury, or
economic loss caused by this product.
References:
1.
2.
3.
4.
5.
6.
7.
Govender D, Harilal P, Dada M, Chetty R. CD31 (JC70)
expression in plasma cells: an immunohistochemical analysis of
reactive and neoplastic plasma cells. J Clin Pathol 1997
Jun;50(6):490-3
Rongioletti F et al. Tumor vascularity as a prognostic indicator
in intermediate-thickness (0.76-4 mm) cutaneous melanoma. A
quantitative assay. Am J Dermatopathol 1996 Oct;18(5):474-7.
Engel CJ, Bennett ST et al.. Tumor angiogenesis predicts
recurrence in invasive colorectal cancer when controlled for
Dukes staging. Am J Surg Pathol 1996 Oct;20(10):1260-5.
Russell Jones R et al. Staining for CD31 and CD34 in Kaposi
sarcoma. Virchows Arch 1996 Jul;428(4-5):217-21.
Poblet E et al. Different immunoreactivity of endothelial
markers in well and poorly differentiated areas of
angiosarcomas. J Clin Pathol 1995 Nov;48(11):1011-6.
Hudock J, Chatten J, Miettinen M. Immunohistochemical
evaluation of myeloid leukemia infiltrates (granulocytic
sarcomas) in formaldehyde-fixed, paraffin-embedded tissue. Am
J Clin Pathol. 1994 Jul;102(1):55-60.
Parums DV, Mason DY et al. JC70: a new monoclonal antibody
that detects vascular endothelium associated antigen on
routinely processed tissue sections. J Clin Pathol 1990
Sep;43(9):752-7.
BIOCARE Products
Catalog # Size
Product
Description
CB911M
500ml
NULCEARDECLOAKER

Assure Technology
A 10X Tris buffer formulation, pH 9.5. Unmasks or
decloaks epitopes crosslinked by formalin fixation
(Color-coded with pH indicator)
DC2000
1 each
Decloaking Chamber
Electric pressure cooker for HIER methods (Superior to
microwave methods)
PD900L
100ml
IgGCARE
Color-coded antibody diluent for primary antibodies
PEP956H
25ml
CAREZYME II (Pepsin)
Liquid, ready-to-use. Reagent for digestion techniques.
DRY2000
1 each
Desert Chamber
Drying oven with turbo fan and temperature alarm
system
DS830H
25ml
DABSPARKLE
DAB enhancing solution
SFH1104A
1 gross
Kling-ON HIER Slides
Positive-charged slides for HIER methods (Extra strong)
For Research use only. Not for therapeutic or in vitro diagnostic use.
Revised 2/12/16
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