Diversity of eukaryotic microbes detected by culture-independent molecular
analysis in sediments around fumaroles in Kagoshima Bay, Japan
Kiyotaka Takishita1, Hiroshi Miyake1,2, Masaru Kawato1 and Tadashi Maruyama1
1. Res. Prog. Mar. Biol. Ecol., Japan Agency for Marine-Earth Science and Technology
(JAMSTEC), 2-15 Natsushima, Yokosuka, Kanagawa 237-0061, Japan
E-mail address: tadashim@jamstec.go.jp
2. Enoshima Aquarium, 2-19-1 Katase-Kaigan, Fujisawa, Kangnagawa, 251-0035, Japan
While prokaryotic diversity has been studied in various environments, relatively little is
known about the diversity of eukaryotic microbes (protist). To study their diversity in anaerobic
environments using culture-independent molecular methods, we directly extracted DNA from
anoxic sediment collected near active fumaroles on a submarine caldera floor (in Kagoshima Bay,
Japan) at a depth of 200 m, and constructed genetic libraries of PCR-amplified eukaryotic
small-subunit (SSU) rDNA…(The rest is omitted.)