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Associate
Member Number: 7520050
Mountain View, CA 94043
Portfolio: http://www.beyond.com/people/7520050
Professional Information
Job Function: Science & Biotech, Research, Healthcare & Medical
Education: Post Graduate Degree
Experience: 1 – 3 Years
Employment: Any Position Type
Salary: $60-60k
Security Clearance: Active Secret
Citizenship: Green Card
Resume
Objective
To obtain a position in Research or Development in a Biotechnology or Pharmaceutical company. (Authorized to work in
the USA)
Summary of qualifications
* Master of Biotechnology (August ***) San Jose State University, San Jose, CA.
* ***. Biochemistry University of Madras, Guindy Campus. Chennai, India.
* ***. Zoology Stella Maris College, Chennai, India.
Laboratory Skills
* PCR Mutagenesis, Restriction digestions, Oligo Designs, Ligation-Transformations, PCR Purification, Gel ExtractionPurification, Sequencing results analysis (Chromatogram), Mini-preps, Maxi-preps, ELISAs, Protein Purification, Tissue
Culture work involving Transient Transfections, Stable Transfections using ***H cells and SP2/O cells, Worked with HPBALL cells, Biotin Labeling, FACS, Monoclonal Antibody Production and Hybridoma technology, HPLC,SDS-PAGE
analysis, ***D gel Electrophoresis.
Relevant Experience
* Life Science Research Assistant at Stanford University Medical Center from May *** to July *** (temporary)
* Internship at Protein Design Labs, Inc., Fremont, CA from June *** – August *** in the Protein Engineering Department
Details – Worked on Hinge Engineering Project during using various Molecular techniques, Tissue Culture work, ELISAs
and FACS analysis. Presented Talk and poster to the whole company.
Associate
* Certificate course in Immunology, Molecular Biology, Biochemistry, and Cell Biology at Tuberculosis Research Center,
Chennai, India
* Certificate course in Immunological assays from Foothill college, California
Details - Performed numerous ELISAs and Western Blot analyses.
* Courses in Bioinformatics (Basics and Advanced) during M.S. at SJSU – BLAST, Vector NTI.
* Course in Advanced Immunological techniques during M.S. at SJSU
Project – Monoclonal Antibody Production and Hybridoma Technology
***Details - Injected Listeria monocytogenes antigen into Balb/C mice for antibody production, collected immune
splenocytes, fused with tumor cells. Fused cells were selected, screened, cloned. Learnt cell culture and aseptic
techniques in detail. Performed a number of ELISA assays during the screening process. Primed the Balb/C mice with
pristane for Ascites production. Injected hybridoma cells. Tapped cells from mouse, purified the immunoglobulins by
column chromatography, characterized the antibodies. Purity was determined by SDS-PAGE. Densitometry analysis was
carried out using Kodak Molecular analyst software. Specificity of monoclonal antibody was determined using Immunoblot
analysis. Performed monoclonal antibody isotype determination using Flow cytometry. Recorded data systematically in
lab notebook.
* Course in Advanced Molecular techniques during M.S. at SJSU
Project – Current Molecular Techniques in Detail
***Part I - Model system used – maize plant light regulated genes. Learnt to design PCR primers, Isolated DNA from light
grown and dark grown maize shoots, Quantification of Maize DNA by gel analysis, Optimized the maize gene PCR,
Purified PCR products, Biotinylated the PCR products, Isolated total RNA from maize seedlings, gel analysis of RNA,
performed Northern blot analysis of maize gene expression, performed Hybridization and Chemiluminescent detection of
Northern blot, PCR Trouble shooting PCR and Northern blotting.
***Part II - Extraction and determination of Maize seedling protein, Isolelectric focusing, SDS-PAGE ***D electrophoresis.
***Part III – Designed oligo primers, performed Site-directed mutagenesis of pGEM multiple cloning site, Carried out heatshock transformation and electroporation, Isolated and restricted Plasmid DNA from transformed ***, performed Gel
analysis to confirm restriction, Observed DNA sequencing.
* Final Semester project (during the course of ***. degree) titled “Anti-tubercular Activity of Azadirachta indica (Neem
Plant)”. Performed independent research including defining milestones and achieving them. Also developed exceptional
organization and laboratory practice habits which helped resolve experimental problems and failures.
* Initiated PhD. prior to arrival in United States. For 2 years, performed extensive literature survey and conducted many
types of experiments while investigating the “Anti-tubercular Activity of Traditional Medicinal Plants”. Conducted extensive
literature survey and developed certain microbiology assays. Learnt Centrifugation techniques, Vacuum filtration.
* Courses in Business including Marketing management and Economics & Society as a part of Masters program at SJSU.
* Course in Regulatory affairs during the course of Masters program at SJSU.
* Presented project reports and talks on various Biotechnology related topics during Masters program at SJSU. Also,
exposure to business plan formulation.
* Assisted Dr. David Bieber briefly in some laboratory work during summer of *** Worked on *** plating, counting and
staining, mammalian cell culture techniques.
Other Skills
* Computer Training: Received Professional Diploma in Software Technology and Systems Management (Unix, DOS,
Windows operating systems, HTML, Java, C***, MS Office, Database Concepts) and Diploma in Advanced Java and Web
Technologies.
* Report Writing and Presentation Experience: Submitted a thesis as requirement for ***. degree. Several class
presentations during the course of ***., ***., MBT degrees. Presented Talk and poster to the whole company during
internship at Protein Design Labs., Inc.
References
Associate
Available on request.