OIE Reference Laboratory Reports
Activities in 2010
Name of disease (or topic) for
which you are a designated OIE
Reference Laboratory:
Address of laboratory:
Newcastle disease
Istituto Zooprofilattico Sperimentale delle Venezie (IZSVe),
Viale dell’Università, 10 – 35020 Legnaro (Padua), Italy
Tel.:
+39 049 8084379
Fax:
+39 049 8084360
e-mail address:
icapua@izsvenezie.it
website:
Name of Head of Laboratory
(Responsible Official):
Name of OIE Reference Expert:
Ilaria Capua
Name of writer of this report
(if different from above):
Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010
1
Newcastle disease
Part I: Summary of general activities related to the disease
1.
2.
Test(s) in use/or available for the specified disease/topic at your laboratory
Test
For
Specificity
Total
Haemagglutination inhibition test (HI)
Antibody
Serotype
13480
Haemagglutination inhibition test (HI)
Viral typing
Subtype
324
Inoculation of SPF embryonated chicken eggs
Virus isolation
Group
200
ICPI (Intra-cerebral pathogenicity index)
Viral pathotyping
Pathotype
12
Sequencing
Viral pathotyping
Pathotype
134
rRT-PCR
(reverse transcriptase polymerase chain reaction)
Virus
Group
1050
Production and distribution of diagnostic reagents
In 2010, 26,595 ml of reference antigens and 3,375 ml of reference sera were produced. The list of reagents
produced is shown in detail below:
Avian influenza antigens (H1-H16)
Infectious bronchitis antigens
Newcastle disease antigen
Infectious bursal disease antigen
Avian Paramyxovirus antigens (APMV-1, APMV-9)
Infectious laryngotracheitis antigen
Reovirus antigen
Negative serum
Positive sera for avian influenza virus (H1-H16)
Positive serum for Newcastle disease virus
Positive Avian Paramyxovirus serum (APMV-1, APMV-9)
Infectious bursal disease serum
Infectious laryngotracheitis serum
Reovirus serum
Hepatitis E serum


A total of 2,311 ml of antigens were distributed, of these 1,139 ml nationally and 1,175 ml internationally.
A total of 1,492 ml of sera were supplied, of these 660 ml nationally and 832 ml internationally.
Part II: Activities specifically related to the mandate
of OIE Reference Laboratories
3.
International harmonisation and standardisation of methods for diagnostic testing or the
production and testing of vaccines
Ring trials in which the OIE Italian laboratory participated during 2010
EU serological and virological identification ring trial.
2
Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010
Newcastle disease
Ring trials organized by the OIE Italian laboratory during 2010
National Ring-test
In 2010 a serological and virological ring test was organised by the Italian National Reference Centre to assess the
capability of Italian public laboratories to identify selected antigens and to detect antibodies in avian sera against
avian influenza viruses and Newcastle disease and to standardize the diagnostic procedures used by the different
laboratories.
All the laboratories were able to identify Newcastle disease both by virological and serological methods.
International Ring-test
In addition to the National Ring-Test, the Reference laboratory organized a Ring-test for avian influenza and
Newcastle disease for African reference laboratories, and for laboratories in the Balkan region and Middle East.
This initiative was funded by FAO and by FAO ECTAD (Mali).
All the laboratories correctly identified NDV both by virological and molecular methods. This proficiency test was
essential in providing the necessary feedback to FAO, donors and reference laboratories to better design future
training courses and provide technical support to the network.
4.
Preparation and supply of international reference standards for diagnostic tests or vaccines
See point 2 above.
5.
Research and development of new procedures for diagnosis and control
None.
6.
Collection, analysis and dissemination of epizootiological data relevant to international disease
control
All diagnoses of ND in Member countries performed at IZSVe were notified to the OIE in a timely manner.
7.
Provision of consultant expertise to OIE or to OIE Members
None.
8.
Provision of scientific and technical training to personnel from other OIE Members
During 2010 IZSVe continued and increase training activities on avian influenza and Newcastle disease. In
particular, it managed training courses within the framework of European projects (EPIZONE, FLUTRAIN), and
organized training courses on major avian diseases for veterinarians and technicians. The list of visitors is reported
in Table 2.
Table 2: List of people attending courses at IZSVe during 2010
Name
Laboratory
Country
Training subject
Rosa-Stella Mbulu
Central Veterinary Laboratory,
Ministry of Agriculture, Water
and Forestry
Namibia
Diagnosis of AI and NDV
15/02/2010
26/02/2010
Canaan Tinashe
Department of Veterinary
Technical Services, Ministry of
Agriculture
Zimbabwe
Diagnosis of AI and NDV
15/02/2010
26/02/2010
Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010
Period
3
Newcastle disease
Name
Laboratory
Country
Training subject
Wegdan Hassan Ali Abdel
El Mageed
Central Veterinary Research
Laboratories, Department of
Virology
Sudan
Diagnosis of AI and NDV
15/02/2010
26/02/2010
Maria Lucia Mandola
Istituto Zooprofilattico
Sperimentale del Piemonte,
Liguria e Valle d’Aosta
Italy
Diagnosis of major avian
diseases-Laboratory quality
assurance
07/04/2010
08/04/2010
Tony Joannis
National Veterinary Research
Institute
Nigeria
Research studies
01/09/2009
30/05/2010
Jeffrey Christ
Virginia-Maryland Regional
College of Veterinary Medicine
United
States
Diagnosis of AI and NDV
01/06/2010
08/08/2010
Yulian Dimitrov Tumbarsky
National Diagnostic and
Research Veterinary Medical
Institute (NDRVMI)
Bulgaria
FLUTRAIN projectDiagnosis of ND-ND
epidemiology and control
strategies
04/10/2010
14/10/2010
Hesamodin Emadi Chashmi
Department of Clinical Sciences,
Faculty of Veterinary Medicine
Iran
Diagnosis of AI and major
avian diseases
18/10/2010
18/04/2011
Laìs Pancotto
Universidade Luterana Do
Brasil
Brazil
Diagnosis of AI and major
avian diseases
25/10/2010
24/12/2010
9.
Period
Provision of diagnostic testing facilities to other OIE Members
Table 3 shows the analyses performed at IZSVe for NDV in 2010 for OIE Members.
Table 3: Samples from OIE Members submitted for Newcastle disease diagnosis at IZSVE
Country
Serological tests
Virological tests
Molecular tests
Total
BANGLADESH
0
0
4
4
BULGARIA
0
1
16
17
EGYPT
1
88
142
231
JORDAN
0
0
6
6
IRAN
0
0
28
28
LITHUANIA
0
0
308
308
PAKISTAN
0
4
297
301
TURKEY
0
2
2
4
UNITED KINGDOM
0
7
1
8
ZIMBAWE
0
0
417
417
Total
48
114
1581
1743
10. Organisation of international scientific meetings on behalf of OIE or other international bodies
None.
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Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010
Newcastle disease
11. Participation in international scientific collaborative studies
None.
12. Publication and dissemination of information relevant to the work of OIE (including list of
scientific publications, internet publishing activities, presentations at international conferences)
1.
Cattoli G., Fusaro A., Monne I., Molia S., Le Menach A., Maregeya B., Nchare A., Bangana I., Garba Maina
A, N'Goran Koffi J-N, Thiam H., Bezeid O E M. A., Salviato A., Nisi R., Terregino C., Capua I. (2010).
“Emergence of a New Genetic Lineage of Newcastle Disease Virus In West And Central Africa –
Implications For Diagnosis And Control”. Vet Microbiol., 19;142(3-4):168-76.
13. Inscription of diagnostic kits on the OIE Register
i)
Did you participate in expert panels for the validation of candidate kits for inscription on the
OIE Register? If yes, for which kits?
No
ii)
Did you submit to the OIE candidate kits for inscription on the OIE Register? If yes, for
which kits?
No
_______________
Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010
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