Raymond Victor Barbehenn
University of Michigan
Ann Arbor, MI 48109-1048
(734) 764-2770
Born:
25 May 1959, Washington, D.C.
Education:
Oberlin College (B.A.; Biology, Environmental Studies; 1981).
SUNY at Stony Brook (Department of Ecology and Evolution; 1982-1983;
transferred).
UC Berkeley (Ph.D.; Department of Entomological Sciences; 1989).
Research:
Associate Research Scientist. University of Michigan, 2006-present. Plant
oxidative defenses. Tannin oxidative activity in caterpillars and grasshoppers.
Assistant Research Scientist. University of Michigan, 2000-2006. Plant oxidative
defenses: effects of ingested oxidases and phenolic compounds on caterpillar gut
biochemistry and insect performance. Phenolic compounds as prooxidants. Insect
nutritional ecology.
Research Investigator. University of Michigan, 1994-1999. Oxidative stress in
insect herbivores: roles of antioxidant enzymes and antioxidants in the gut lumen.
The peritrophic envelope as a barrier to ingested plant allelochemicals.
Postdoctoral Fellow. Dr. Michael M. Martin, University of Michigan, 1990-1993.
Biochemical and physiological effects of ingested tannins on larval Lepidoptera.
Postdoctoral Fellow. Dr. Alexander Purcell, UC Berkeley, 1990. Factors affecting
insect transmission of a xylem-inhabiting bacterium, Clavibacter xyli cynodontis, to
grasses by insects.
Doctoral Dissertation Research. Dr. Elizabeth Bernays, UC Berkeley, 1984-1989.
Nutritional ecology of C3 and C4 grass-feeding caterpillars and ultrastructural
changes in C4 grass leaf tissues during digestion.
Teaching:
Undergraduate Research Advisor. MCDB 300, 400 & 700 (15 students 20002008, including three honors students).
Undergraduate Research Mentor. NSF REU (six fellowships 2000-2003).
Undergraduate Research Mentor. UM UROP and MCDB research support (eight
fellowships 2001-2008).
Instructor. Summers-Knoll Elementary School summer field course on insects
(2001-2006) and guest lecturer in entomology.
Naturalist. Ann Arbor Public Schools, Environmental Ed. Program, 1996-present.
Volunteer. UM Museum of Natural History, Science outreach program (K-12),
1995-1996.
Teaching Assistant. Applied Entomology (UC Berkeley, 1989), General
Entomology (UC Berkeley, 1986), Introductory Biology (UC Berkeley, 1984, 1985;
SUNY at Stony Brook, 1982, 1983), Plant Systematics (Oberlin College, 1982), The
Fungi, Oberlin College, 1981, 1982).
Awards,
Grants,and
Fellowships:
Publications:
USDA Research Grant (PI); 2006-2009 ($391,000).
USDA Research Grant (PI); 2004-2007 ($376,000).
USDA Research Grant (PI); 1999-2002 ($200,000).
NSF Research Grant (PI); 1999-2002 ($322,000).
NSF Research Grant (PI); 1997-1999. ($72,000).
NSF Research Grant (Co-PI); 1994-1997 ($230,000).
University of California Newhouse Fund Grant; 1989.
Harvey Magy Memorial Scholarship in Entomology; 1988.
NSF Doctoral Dissertation Improvement Grant; 1987-1989.
University of California Regents Fellowship; 1987.
Sigma Xi Research Grant; 1985.
Inter-campus Doctoral Fellowship (SUNY College of Env. Sci. & Forestry); 1983.
NSF Undergraduate Research Program; 1981.
Mountain Lake Biological Station Naturalist Award; 1981.
Barbehenn R.V., R.E. Maben and J.J. Knoester. 2008. Linking phenolic oxidation in the
midgut lumen with oxidative stress in the midgut tissues of a tree-feeding caterpillar
Malacosoma disstria (Lepidoptera: Lasiocampidae) Environmental Entomology (in
press).
Barbehenn R.V., A. Jaros, L. Yipp, L. Tran, A.K. Kanellis, and C.P. Constabel. 2008.
Evaluating ascorbate oxidase as a plant defense against leaf-chewing insects using
transgenic poplars. J. Chem. Ecol. (in press).
Constabel C.P. and R. Barbehenn. 2008. Defensive roles of polyphenol oxidase in plants.
In A. Schaller (ed.), Induced Plant Resistance to Herbivory. Springer, New York.
Barbehenn R.V., Weir Q., and J.-P. Salminen. 2008. Oxidation of ingested phenolics in
the tree-feeding caterpillar Orgyia leucostigma depends on foliar chemical
composition. J. Chem. Ecol. 34: 748-756.
Barbehenn R.V., C.P. Jones, L. Yip, L. Tran, and C.P. Constabel. 2007. Limited impact
of elevated levels of polylphenol oxidase on tree-feeding caterpillars: assessing
individual plant defenses with transgenic poplar. Oecologia 154: 129-140.
Barbehenn R.V., C.P. Jones, A.E. Hagerman, M. Karonen and J.-P. Salminen. 2006.
Ellagitannins have greater oxidative activities than condensed tannins and galloyl
glucoses at high pH: potential impact on caterpillars. J. Chem. Ecol. 32: 2253-2267.
Barbehenn R.V., C.P. Jones, M. Karonen and J.-P. Salminen. 2006. Tannin composition
affects the oxidative activities of tree leaves. J. Chem. Ecol. 32: 2235-2251.
Barbehenn R.V. 2005. Grasshoppers efficiently process the bundle sheath cells in a C4
grass: implications for patterns of host plant utilization. Entomol. Exp. Appl. 116:
209-217.
Barbehenn R., T. Dodick, U. Poopat and B. Spencer. 2005. Fenton-type reactions and
iron concentrations in the midgut fluids of tree-feeding caterpillars. Arch. Insect
Biochem. Physiol. 60: 32-43.
Barbehenn R.V., S. Cheek, A. Gasperut, E. Lister and R. Maben. 2005. Phenolic
compounds in red oak and sugar maple leaves have prooxidant activities in the
midguts of Malacosoma disstria and Orgyia leucostigma caterpillars. J. Chem. Ecol.
31: 969-988.
Barbehenn R.V. and J. Stannard. 2004. Antioxidant defense of the midgut epithelium by
the peritrophic envelope in caterpillars. J. Insect Physiol. 50: 783-790.
Barbehenn R.V., Z. Chen, D.N. Karowe and A. Spickard. 2004. C3 grasses have higher
nutritional quality than C4 grasses under ambient and elevated atmospheric CO2.
Global Change Biol. 10: 1565-1575.
Barbehenn R.V., D.N. Karowe and Z. Chen. 2004. Performance of a generalist
grasshopper on a C3 and a C4 grass: compensation for the effects of elevated CO2 on
plant nutritional quality. Oecologia 104: 96-103.
Barbehenn R.V., D.N. Karowe and A. Spickard. 2004. Effects of elevated atmospheric
CO2 on the nutritional ecology of C3 and C4 grass-feeding caterpillars. Oecologia
104: 86-95.
Barbehenn R.V., A.C. Walker and F. Uddin. 2003. Antioxidants in the midgut fluids of a
tannin-tolerant and a tannin-sensitive caterpillar: effects of seasonal changes in tree
leaves. J. Chem. Ecol.29: 1099-1116.
Barbehenn, R.V. and N.P. Kristensen. 2003. Digestive and excretory system. In N. P.
Kristensen (ed.), Lepidoptera: Moths and Butterflies 2. Handbook of Zoology. 4 (36).
De Gruyter, New York.
Barbehenn, R.V. 2003. Antioxidants in grasshoppers: higher levels defend the midgut
tissues of a polyphagous species than a graminivorous species. J. Chem. Ecol. 29:
683-702.
Barbehenn, R.V., U. Poopat and B. Spencer. 2003. Semiquinone and ascorbyl radicals in
the gut fluids of caterpillars measured with EPR spectrometry. Insect Biochem. Mol.
Biol. 33: 125-130.
Barbehenn, R.V. 2002. Gut-based antioxidant enzymes in a polyphagous and a
graminivorous grasshopper. J. Chem. Ecol. 28: 1325-1343.
Barbehenn, R.V. 2001. Roles of peritrophic membranes in protecting herbivorous insects
from ingested plant allelochemicals. Arch. Insect Biochem. Physiol. 47: 86-99.
Gongora, C.E., S. Wang, R.V. Barbehenn and R.M. Broadway. 2001. Chitinolytic
enzymes from Streptomyces albidoflavus expressed in tomato plants: effects on
Trichoplusia ni. Entomol. Exp. Appl. 99: 193-204.
Barbehenn, R.V., S.L. Bumgarner, E. Roosen, and M.M. Martin. 2001. Antioxidant
defenses in caterpillars: role of the ascorbate recycling system in the midgut lumen. J.
Insect Physiol. 47: 349-357.
Johnson, K.S. and R.V. Barbehenn. 2000. Oxygen levels in the gut lumens of herbivorous
insects. J. Insect Physiol. 46:897-903.
Barbehenn, R.V. 1999. Non-absorption of ingested lipophilic and amphiphilic
allelochemicals by generalist grasshoppers: the role of extractive ultrafiltration by the
peritrophic envelope. Arch. Insect Biochem. Physiol. 42: 130-137.
Barbehenn, R.V., J. Reese and K.S. Hagen. 1999. The food of insects. In C. B. Huffaker
and A.P. Gutierrez, eds., Ecological Entomology. John Wiley and Sons, New York,
pp. 83-122.
Barbehenn, R.V. and M.M. Martin. 1998. The formation of insoluble and colloidally
dispersed tannic acid complexes in the midgut fluid of Manduca sexta (Lepidoptera:
Sphingidae): an explanation for the failure of tannic acid to cross the peritrophic
envelopes of lepidopteran larvae. Arch. Insect Biochem. Physiol. 39: 109-117.
Barbehenn, R.V. and M.M. Martin. 1997. Permeability of the peritrophic envelopes of
herbivorous insects to dextran sulfate: a test of the polyanion exclusion hypothesis. J.
Insect Physiol. 43: 243-249.
Barbehenn, R.V., M.M. Martin and A.E. Hagerman. 1996. Reassessment of the roles of
the peritrophic envelope and hydrolysis in protecting polyphagous grasshoppers from
ingested hydrolyzable tannins. J. Chem. Ecol. 22: 1911-1929.
Barbehenn, R.V. 1995. Measurement of protein in whole plant samples with ninhydrin. J.
Sci. Food Agr. 69: 353-359.
Barbehenn, R.V. and M.M. Martin. 1995. Peritrophic envelope permeability in
herbivorous insects. J. Insect Physiol. 41: 303-311.
Barbehenn, R.V. 1994. Host plants of Poanes melane (Hesperiidae). J. Lepidopt. Soc. 48:
386-388.
Barbehenn, R.V. and M.M. Martin. 1994. Tannin sensitivity in Malacosoma disstria:
roles of the peritrophic envelope and midgut oxidation. J. Chem. Ecol. 20: 19852001.
Barbehenn, R.V. and A.H. Purcell. 1993. Factors affecting the transmission of a xyleminhabiting bacterium, Clavibacter xyli cynodontis, to grasses by insects.
Phytopathology 83: 859-863.
Barbehenn, R.V. 1993. Silicon: an indigestible marker for measuring food consumption
and utilization by insects. Entomol. Exp. Appl. 67: 247-251.
Barbehenn, R.V. and M.M. Martin. 1992. The protective role of the peritrophic
membrane in the tannin-tolerant larvae of Orgyia leucostigma (Lepidoptera). J.
Insect Physiol. 38: 973-980.
Barbehenn, R.V. and E.A. Bernays. 1992. Relative nutritional quality of C3 and C4
grasses for a graminivorous lepidopteran, Paratrytone melane (Hesperiidae).
Oecologia 92: 97-103.
Barbehenn, R.V. 1992. Digestion of uncrushed leaf tissues by leaf-snipping larval
Lepidoptera. Oecologia 89: 229-235.
Barbehenn, R.V. and A. Keddie. 1992. Gut contents in molting larval Lepidoptera: a
source of error in nutritional studies. Entomol. Exp. Appl. 62: 87-91.
Montllor, C.B., E.A. Bernays and R.V. Barbehenn. 1990. Importance of quinolizidine
alkaloids in the relationship between larvae of Uresiphita reversalis (Lepidoptera:
Pyralidae), and a host plant Cytisus monspessulanus. J. Chem. Ecol. 16: 1853-1865.
Usher, B.F., E.A. Bernays, R.V. Barbehenn and R.P. Wrubel. 1989. Oral dosing of insects
with feeding deterrent compounds. Entomol. Exp. Appl. 52: 119-133.
Usher, B.F., E.A. Bernays and R.V. Barbehenn. 1988. Antifeedant tests with larvae of
Pseudaletia unipuncta: variability of behavioral response. Entomol. Exp. Appl. 48:
203-212.
Bernays, E.A. and R.V. Barbehenn. 1987. Nutritional ecology of grass foliage-chewing
insects. In Slansky, F. and J.G. Rodriguez, eds., Nutritional Ecology of Insects,
Mites, and Spiders. John Wiley and Sons., New York.