Chapter 12 DNA Analysis Checkpoint Answers 1. In the nucleus of the cell 2. Wrapped around the chromosomes 3. A gene is a smaller portion of the chromosome, both of which are portions of the DNA molecule that is packed into the nucleus. 4. The Human Genome Project is a unified effort to identify and determine the sequence of all genes found on the human chromosome. 5. The nucleus 6. Adenine, guanine, cytosine, thymine 7. The phosphate groups give DNA its acidic properties. 8. Blood, semen, saliva, hair follicular tissue, bone 9. Restriction Fragment Length Polymorphism 10. To cut the DNA into smaller fragments 11. Probably not; the sequence may be different, but the number of base pairs may be the same, making the fragment length the same. 12. PCR can make many copies of DNA, which can then undergo characterization and analysis. 1 13. In RLFP, between sequences; in PCR, lengthwise between bases (“unzipped”) 14. In RFLP, with restriction enzymes; in PCR, heat. 15. To begin the replication in the process of PCR 16. To identify shorter sequences after RFLP has been performed 17. The FBI Lab’s Combined DNA Index System (CODIS) is used by law enforcement agencies. 18. The forensic index is a DNA database formed with DNA from crime scenes; the offender index is a DNA database formed with the DNA of criminal offenders. 19. Mitochondrial DNA comes from the mitochondria in the cell. It can be obtained from older or more degraded and/or smaller samples. 20. Nuclear DNA is inherited from both mother and father; mitochondrial DNA is inherited from the mother only. 2