Salman Bin Abdul Aziz UNIVERSITY
COLLEGE OF APPLIED MEDICAL SCIENCES
DEPARTMENT OF Medical Laboratory Science
Female Sector
COURSE SYLLABUS
Course Title:
Gene technology and molecular techniques
Course Number:
MLAB 475
Credit Hours:
3
Course Instructors: Dr /Noura Al-dayan
Course Description: This course is concerned with theoretical and practical knowledge of Gene
technology and molecular techniques
Course objectives:
Upon successful completion of this course, the student should be able to:
1- Understand the essential knowledge and information about various molecular biology techniques
such as purification of nucleic acids, restriction enzymes, cloning of DNA fragments, DNA
&RNA labelling, probes, analysis of DNA & RNA by electrophoresis, blotting and hybridization
& DNA sequencing.
2- Identify different techniques used in molecular biology such as polymerase chain reaction (PCR)
and the related technologies in diagnosis, ways and means of delivering genes.
3- Learn various molecular biology and gene technology basic concepts as bacterial transformation,
vectors and plasmid, host cell types, Alternative methods for DNA delivery, gene expression,
isolation of DNA and RNA, blotting and hybridization, reverse transcription, and PCR and
cloning DNA fragments.
4- Instructed in the theory, practice and applications of various molecular biology techniques such as
purification of nucleic acids, cloning of DNA fragments, DNA & RNA electrophoresis, blotting
and hybridization, PCR & DNA Sequencing.
5- Understand the applications and values of these techniques in the investigation of genetic
diseases-as well as histocompatibility testing in transplantation and bone marrow engraft-will be
emphasized.
1
Course Outline:
Week 1: Introduction: DNA structure, DNA functions, DNA replication, RNA structure, RNA functions,
Genetic code & Proteins synthesis.
Week 2:
Restriction Enzymes.
Week 3:
DNA Manipulative Enzymes.
Week 4:
Control of Gene Expression
Week 5:
Gene (DNA) Cloning.
Week 6:
Host cell types & Vectors transfer DNA for
Recombinant DNA Technology.
Week 7:
DNA & RNA Electrophoresis.
Week 8:
DNA & RNA labelling (Nucleic acid labelling).
Week 9:
DNA Sequencing.
Week 10:
Hybridization techniques
Week 11:
hybridization
Hybridization techniques: Southern and Northern Blotting & Fluorescent in situ
Week 12:
Histocompatibility testing in transplantation & Bone marrow engraft (Genetics role in
transplantation)
Week 13:
Investigation of genetic diseases (Genetic Screening).
Week 14:
Revision.
Teaching Methods:
1- Lectures using visual aids
2- Class discussion
Grading:
- Midterm Examination: First and second (12%+12%), Course work (3%- 3%)
- Final practical (30%)
- Final examination (Theoretical 40%)
2
References:
REQUIRED TEXTBOOK:
1. Introduction to molecular genetics and genomics by Hearts.
2. Molecular biology of the gene fifth edition by James D. Watson (2003).
3. Molecular Biology, Robert Weaver, WBC McGraw Hill, NY. 2001, ISBN 00072345179.
4. Genetics principles and analysis by Paniel L. Hartl. Publisher: Gareth Stevens Publishing; 4th edition
(January 15, 1998) | ISBN: 076370489X.
Laboratory Manuals:
1. Molecular Cloning: A Laboratory Manual, J. Sambrook, P. MacCallum, D. Russell, Cold Spring
Harbor Laboratory Press, Cold Spring Harbor, NY. (3rd Edition), 2001, ISBN 0-87969-577-3.
2. PCR strategies 1995 Michael A. Innis, David H. Gelfand, and John J. Sninsky (eds.).Academic Press,
San Diego.
3. Gel electrophoresis of nucleic acids: a practical approach. 1990. D. Rickwood and B. D. Hames. Oxford
University Press, New York.
4. DNA cloning: a practical approach. 1995. D. M. Glover and B. D. Hames (eds.) Oxford University
Press, New York.
3