Class Notes Human Genome Project, Gene Therapy, & Cloning Questions/Main Idea: How much DNA is in one cell? What is the Human Genome Project? What were the goals of the human genome project? How did they do it? Name: _______________________________________ Period:_______________________________________ Date: _______________________________________ Notes: Human genome = 46 chromosomes about 3 billion base pairs one base pair is 0.00000000034 meters DNA sequence in any two people is 99.9% identical – only 0.1% is unique! An international effort to decipher the DNA blueprint of a human being To sequence and determine the exact order of nucleotides (A,T,C,G) for all of the DNA in a human cell To determine which sections of DNA represent the individual genes To store this information in databases for analysis 1. DNA was collected from 1000s of volunteers 2. Samples were sent to Human Genome Project centers around the world 3. Scientists at centers perform DNA sequencing and analysis Compare the genome of humans with other organisms Discover DNA and its functions Investigate the interactions between DNA sequences, gene products, and environmental factors Analyze genetic variation between individuals and populations How can we use the information? Better understanding of human disease Personalized medicine Greater insight into the brain Insight into human origins Identify genetic susceptibility to disease What is Gene Therapy? Replacement of an abnormal protein with a normal one How does Gene Therapy Work? 1. Isolate the normal sequence for the protein and package it into a virus (vector) 2. Infect a target cell, usually the one with the illness, such as a liver or lung 3. The virus uses the normal sequence to produce the missing protein and the cells return to normal 4. Lasts the life of the cell then patient must be retreated. What are the problems with Gene Therapy? What is there left to do? Short lived, so patients have to go through multiple rounds Immune response to foreign invaders Problems with the viruses Very expensive Multigene disorders What is cloning? Cloning is the creation of an organism that is the EXACT genetic copy of the mother Identical twins are natural clones, they share 100% of their DNA 1. Remove the nucleus from an egg cell and a regular cell from a donor. 2. Fuse the enucleated cell with a regular cell by electric shock 3. The new fused cell begins dividing, just as a fertilized egg would 4. The embryo is placed in the uterus of a foster mother and allowed to develop normally 5. The foster mother gives birth to a genetic clone of the original donor! How do you clone an organism? Why might we want to clone? For medical purposes Revive endangered species or extinct species Reproduce deceased pets Any reason to clone a human? What are the risks of cloning? High failure rate Problems later in development Premature aging Abnormal gene expression Summary: