Supplementary Data
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2
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Supporting Tables
TABLE S1 Growth media composition and source of carbon into and out of cellulosic
saccharification process plus carbon contents of each source.
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Supporting Figures
FIG. S1 Scanning electron micrograph of Shigella flexneri strain G3 cells grown on Avicel
mineral salt medium with 0.15% yeast extract at pH 7.0 and 37 ºC for 3 days.
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Fig. S2 Cellulase activity in the pellet (a) and in the culture supernatant (b) when Shigella
flexneri G3 grew at various initial concentrations (g L-1) of on Avicel.
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Fig. S3 HPLC chromatogram of the Avicel hydrolysate.
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Fig.S4 Kinetics of defined carbon sources (1 g L-1 of glucose and xylose) utilization over
time by Shigella flexneri strain G3.
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1
2
3
FIG. S1 Scanning electron micrograph of Shigella flexneri strain G3 cells grown on Avicel
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mineral salt medium with 0.15% yeast extract at pH 7.0 and 37 ºC for 3 days. The bars represent
2.00 μm.
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6
7
2
(a
4
5.0
3.5
4.0
3
3.0
2
2.5
2.0
1
1.0
0
2.5
1.0
2.0
2.0
(b
-1
-1
Cellulase activity in supernatant, 10 U mL
-1
1
Cellulase activity in pellet, 10- U mL
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1
2
3
4
2.5
1.5
3.0
1.0
3.5
0.5
0.0
4.0
5.0
0
20
40
60
80
100
120
140
Time, h
Fig. S2 Cellulase activity in the pellet (a) and in the culture supernatant (b) when Shigella
flexneri G3 grew at various initial concentrations (g L-1) of on Avicel. Data are presented as mean
± standard deviation (error bars ) with triplicates.
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3
1
2
43
--
1
2
3
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Fig. S3 HPLC chromatogram of the Avicel hydrolysate. 1, cellobiose; 2, glucose; 3, cellotriose; 4,
cellotetarose. Column: HPX 87H with ion-exclusion micro-guard (cation H); mobile phase: 4
mM H2SO4; flow rate: 0.6mL/min; column temperature: 70oC; detection: RI.
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4
-1
300
200
100
0
600
500
400
300
200
100
0
0
1
2
3
4
5
10
20
30
40
50
Glucose concentration, g L
Volatile fatty acids, mg L
-1
400
-1
500
1.0
0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0.0
1.0
0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0.0
60
Xylose concentration, g L
600
Time, h
Fig.S4 Kinetics of defined carbon sources (1 g L-1 of glucose and xylose) utilization over time by
Shigella flexneri strain G3. ☆, glucose; ◇, xylose ●, acetate; △, butyrate; □, lactate. Data are
presented as mean ± standard deviation (error bars) with triplicates.
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1
2
TABLE.S1. Growth media composition and source of carbon into and out of cellulosic
saccharification process plus carbon contents of each source.
Component
In
Out
Carbon contents
(%, wt/wt)
Basal Salt
KH2PO4
-
Na2HPO4·12H2O
-
NH4Cl
-
MgCl2·6H2O
-
Nutrients Source
yeast extracta
X
28.3
X
43.2
Carbon Source
Avicel PH-101
Cell massb
X
53.1
Soluble protein
X
50.0
Glucosec
X
40.0
Cellobiosec
Fermentative products
X
43.0
CO2
c
X
27.27
VFAc
X
40.0 of acetate, 48.6 of
propionate,
54.5
butyrate
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a
A nutrient source for a wide variety of industrial and food fermentations and a rich source of amino nitrogen and
other biologically active substances. Crude protein =38.0% dry basis, nitrogen=8.7% dry basis. bCell composition
C5H7NO2. c Carbon content calculated from elemental formulas.
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of