Seminar L11- Laboratorija za molekularno biologijo in nanobiotehnologijo
in
Infrastrukturnega centra za raziskave molekulskih interakcij, Oddelek za
biologijo, Biotehniška fakulteta, Univerza v Ljubljani
Beate Kern
NanoTemper Technologies GmbH, Munich, Germany
Biomolecular Interaction Analytics with
NanoTemper Technologies
Seminar bo v torek 14. 4. 2015 ob 9:30. uri v Preglovi predavalnici
(PRC) Kemijskega inštituta
Povzetek seminarja
This presentation gives a brief overview on Microscale Thermophoresis (MST), a new
technology to study biomolecule interactions. The term Microscale Thermophoresis refers to
the directed movement of molecules in optically generated microscopic temperature gradients.
This thermophoretic movement is determined by the entropy of the hydration shell around the
molecules. Microscale Thermophoresis allows quantification of binding affinities of protein-,
nucleic acid- and small molecule-interactions. The steep microscopic temperature gradient is
generated by an IR-Laser, which is strongly absorbed by water. The readout method of the
interaction analysis is based on fluorescence: fluorescently labeled proteins/peptides/nucleic
acids can be used as well as GFP/YFP/RFP fusion-proteins. In this presentation we will
briefly describe the technical details and the benefits of the Microscale Thermophoresis
technology platform – the aspects will be given as a published examples of groups that used
the technique with the emphasis of membrane protein interactions. In addition the new
technologies, NanoDSF and SAW, will be presented as a novel approaches of protein
characterization.
VLJUDNO VABLJENI!