It is known that stress-mediated factors provide remarkable influence on gut microflora. Large number of investigations suggests dramatic changes of space crew microflora before the flight, when the share of conventional pathogens in intestinal microflora increases about 4 times in comparison with remote (60 day before flight) data. The same tendencies were revealed in other cases when humans are in condition of artificial environment (saturation divers, caisson workers, humans in confined habitat of in other conditions, dealt with stress (sportsmen etc.). As a result of microbial disbalance in the gut, we are facing with, besides of pathogens overgrowth in intestine, translocation of intestinal bacteria to the high respiratory tract, dental system and mouth, where they can be causative agents of inflammation.
In case when the environment itself suggests selection of pathogenic forms of microflora application of biocides is harmful, since it can cause also selection of resistant pathogens, thus application of probiotics is one and only acceptable mean for microbial control.
It is known, that several microbial groups forms colonial resistance of intestinal biotope, e.g. lactobacilli, bifidobacteria, Enterococcus faecium . For many years in IBMP has been studied the influence of autoprobiotics based on Enterococcus faecium, Lactobacillus sp.
and
Bifidobacterium sp.
on intestinal microflora of humans. The results revealed much higher high activity of autoprobiotics in comparison of probiotics, prepared on the background of collection strains. Also, for several recent years in IBMP there was studied influence of probiotics based on
Corynebacterium hoffmani on the status of nasal and throat microflora of humans in confined habitat. It was revealed by many previous studies that long-term presence of humans in confined habitat suggest dramatic increasing of pathogenic pattern of microflora and suppression of protection groups. The application of probiotics based on Corynebacterium hoffmani suggested active quantitative elimination of S.aureus to complete eradication from nose and throat.
The main objectives: To evaluate complex prophylactic activity of autoprobiotic, based on
Enterococccus faecium in combination with probiotics based on Veilonella sp., Corynebacterium pseudodyphtericum (hofmani) for sanation of human microflora of different age groups. To characterize normal microbiota of intestinal tract in terms of aging and pathology.
It is also known that periodontal pathogens could also be withdrawn with the aid of probiotics, based on Veillonella sp.
, meanwhile periodental pathogens are known to colonise actively cardial valves, suggesting therefore the risk of cardial disorders.
Planning to carry out R&D to reveal characteristic changes in intestinal microbiota, related to aging and to control them with use of probiotics and functional food.

Duration 3 years.
Main objectives
- Investigations of state of art, selection of Veilonella strains. Preparation of probiotics.
Deliverable – survey on state-of-art, containing existing knowledge on nasal, throat and periodontial probiotics and experience of IBMP in the field of prophylaxis of infection with the aid of probiotics.
- Study of activity of autoprobiotic, based on Enterococccus faecium in combination with probiotics based on Veilonella sp., Corynebacterium pseudodyphtericum (Hofmani) for sanation of human microflora for prevention of periodontial, nose and throat infections of volunteers – participants of experiments, performed in IBMP. Deliverable – annual report.
- Study of activity of autoprobiotic, based on Enterococccus faecium in combination with probiotics based on Veilonella sp., Corynebacterium pseudodyphtericum (Hofmani) for sanation of human microflora for prevention of periodontial, nose and throat infections of volunteers – participants of experiments, performed in IBMP. Deliverable the final report, patents and publications.
- Development of new probiotics for normalization of intestinal microbiota.
Studies on the viability and reproduction of of different microorganisms with probiotic activity in different parts of intestinal tract.
- Recommendations for the application of functional food composition and probiotics for in habitants of hermetic zones (submarines, space satellites, etc.).
IBMP possesses contemporary laboratory basis – boxes, autoclaves, contemporary identification systems (Vitec), equipment for preparation and storage of strains collection (-80 o C freezers), centrifuge, bioreactors and all other necessary facilities.
The system of artifical intestine imitation of intestine with facilities of dynamic analysis of intestinal microbiota is available.
Postgraduate: Morozova Julia Alekseevna, date of birth 03.03.80. Graduated in 2010 from
Moscow Medical University. Work in IBMP since 2000. Actively participate in all the experimental programs, carried out in the department, headed by Prof. Ilyin.
Research groups from IBMP will participate the Project, headed by Prof. V. Ilyin and
Dr. N.Novikova – totally 6 persons.
Literature references
Viacheslav Ilyin, Natalia Kiriukhina, Julia Morozova Experience of microbial implantation for prophylaxis of Staphylococcus aureus colonization of nasal mucous in experiment on human isolation. Abstracts of 19th IAA Humans in Space Symposium, Cologne, Germany, 2013, P. 274
Viacheslav Ilyin, Nonna Usanova, Liubov Starkova, Julia Morozova, Alexander Suvorov,
Autoprobiotics in prevention of intestinal disbacteriosis of operators in long-term experiment in

confined habitat Abstracts of 19th IAA Humans in Space Symposium, Cologne, Germany,
2013, P. 275
N. V. Kiryukhina, V. G. Melnikov, A. V. Suvorov, Yu A. Morozova, V. K. Ilyin Use of
Corynebacterium pseudodiphtheriticum for elimination of Staphylococcus aureus from the nasal cavity in volunteers exposed to abnormal microclimate and altered gaseous environment
Probiotics and Antimicrobial Proteins, 2013, V. 5, №4 P. 233-238
/ Head of Department, IBMP prof. V. K. Ilyin
Head of Laboratory, IBMP
06.12.2013
Ilyin Viachheslav
Doctor of medical sciences, professor, Head of
Department of Sanitary chemical and antimicrobial safety,
Russian Federation State Scientific Center
Institute for Biomedical Problems (IBMP).
123007 Khoroshevskoye shosse 76a,
Moscow, Russia
E-mail: ilyin@imbp.ru
Dr. N. D. Novikova