PF-IL ACCESS REQUEST TO THE ISOTOPIC LABELLING PLATFORM Request n° : Part to completed by the applicant: Date of the request: Name of the applicant: Membership of the applicant: IBS PSB Other => Academic industrial Protein name (ORF) : Protein sequence: Protein origin (human, bacteria, etc): Biological context (please explain in a few words the characteristics of the protein) : NMR Application: Interaction study Dynamic study Assignment Other (Specify): Has an access request to the High filed NMR PF been submitted? : Yes No SAMPLE : #1 _ What is the expression vector ? #2 _What is the antibiotic resistance? : #3 _ Preliminary experiments: Please describe which preliminary protein expression tests have been conducted in minimal medium (protonated or deuterated) and the results obtained (e.g. expression conditions, yield in mg of protein/L of culture): #4_GMO: Only security level 1 samples are accepted, please precise your GMO agreement number: Desired deadline to access of the Platform : Within one month Within three months Other, please specify : 1/2 PF-IL ACCESS REQUEST TO THE ISOTOPIC LABELLING PLATFORM Request n° : Part reserved for the PF : Validation of the project : Yes No Remark: The applicant acknowledges that the nature of "service" that can be provided depends on how their protein expresses in minimal protonated or deuterated media. In the case of poorly expressing proteins, only the "solubility tests in M9 protonated media" and "solubility tests in M9 deutérated media" can be performed. Downstream protocols will only be applied if the protein can be expressed under these conditions. Date and PF signature : Date and applicant signature : 2/2